Year: 2019

2020 nPOD Annual Meeting

The Renaissance Tampa International Plaza Hotel 4200 Jim Walter Blvd, Tampa, FL 33607 DOWNLOAD MEETING AGENDA The Network for Pancreatic Organ donors with Diabetes (nPOD) is proud to host its 12th Annual Meeting at the Renaissance Tampa International Plaza Hotel in Tampa, Florida, from February 23-26, 2020. Over the past twelve years, nPOD has made remarkable strides in terms […]

Recruiting Principal Investigators at the IBRI Diabetes Center/Lilly Diabetes Center of Excellence

The Lilly Diabetes Center of Excellence (LDCE) at the Indiana Biosciences Research Institute (IBRI) is recruiting promising young investigators to start their own independent laboratories under excellent research conditions The LDCE is recruiting high-potential Principal Investigators (PIs) at the IBRI in Indianapolis, Ind., who are interested in pancreatic beta cell research, particularly related to iPSC-derived […]

T1D BBQ!

T1D BBQ! Sally Kent and Alberto Pugliese initiated and hosted the T1D BBQ (Big Burning Questions) webinar for the nPOD Autoimmunity T Cell Working Group with Francesco Dotta, Dale Greiner, Bart Roep, and Matthias von Herrath as Discussants. The purpose of this webinar was to discuss ‘T cells as pathogenic effectors in human type 1 […]

Distribution of neuropeptide FF in the human infant pancreas

Improving adipose tissue (AT) insulin resistance and induce adipocyte browning are key therapeutic aims in the management of obesity associated insulin resistance (IR) and in type 2 diabetes (T2DM). Our laboratory has recently identified neuropeptide FF (NPFF) as a pancreatic hormone, which selectively improves IR in the AT and controls AT browning, especially in infancy […]

Persistence of Pancreatic Insulin mRNA Expression and Proinsulin Protein and the Autoantigens GAD, IA-2 and ZnT8 in Type 1 Diabetes Pancreata

The canonical notion that type 1 diabetes (T1D) results following a complete destruction of β cells has recently been questioned as small amounts of C-peptide are detectable in patients with long-standing disease. Previously, we analyzed protein and gene expression levels for proinsulin, insulin, C-peptide, and islet amyloid polypeptide within pancreatic tissues from T1D, autoantibody positive […]

Role of bacterial biofilms in the onset of pancreas inflammation in T1D

Even though the immunopathogenic triggering event(s) in type 1 diabetes (T1D) remain enigmatic, there is accumulating evidence, mainly in rodent models of the disorder, suggesting that pancreas-resident antigen-presenting cells (APC) are activated very early post-natally. This results in the recruitment of innate immune cells like neutrophils into the pancreas even before adaptive immune cells that […]

Using Emerging Techniques to Identify Novel Islet Signatures in Type 1 and Type 2 Diabetes

Though the pancreatic islets are known to play critical roles in both Type 1 diabetes (T1D) and Type 2 diabetes (T2D), the distinct mechanisms are poorly understood. Recent findings demonstrate that chemical heterogeneity exists within the classic islet endocrine cell types, even in the normal/healthy pancreas setting. For example, there are subtypes of beta cells […]

Human T cell repertoire profiling in Type 1 Diabetes

Current strategies of studying islet autoantigen-specific T cells rely heavily on fluorescence labeled tetramers and are limited in the throughput of antigens that can be analyzed simultaneously. The profile of islet autoantigen-specific T cells that can be obtained is limited. We recently developed a new technology, Tetramer associated TCR sequencing (TetTCR-Seq), that is capable of […]

Highly Multiplexed Imaging Mass Cytometry of the Human Pancreas Affected by Type 1 Diabetes

Despite extensive efforts, understanding of the mechanisms of type 1 diabetes (T1D) development in human patients is collectively lacking, particularly with regard to contributions of islet endocrine cells, the immune system, and their interplay in the disorder’s development. Until recently, these questions have been particularly challenging to address due to technical limitations in the number […]

Analysis of cellular proteins in β cells from patients with type 1 diabetes and healthy

Our objective is to identify characteristics of β cells that survive immune attack. Our hypothesis is that these residual cells express immune inhibitory molecules and/or decrease expression of diabetes associated antigens. In addition, these cells may show changes consistent with dedifferentiated β cells. Our studies of protein modifications have also shown that there are post-translational […]

The role of protein kinase C delta in beta-cell death and dysfunction in type 1 diabetes

The goal of this study is identify novel mechanisms of immune-mediated β-cell death and dysfunction such that these mechanisms can be exploited to protect against the onset and progression of T1D. To achieve this goal, we propose the following aims: 1) Determine if reduced electrical coupling, altered calcium signaling, and activation of protein kinase C […]

nPOD Pancreas Slice Group Discussion

DATE: Wednesday, May 22nd, 2019 WEBINAR INFO:  Click here to join WebEx Meeting Meeting Number (access code): 737 898 840 Meeting Password: xhMMM3CM Call-in toll number (US/Canada): 1-650-479-3208 Global Call-in Numbers Can’t join the meeting? TIME: 11:00am – 02:00pm EST Discussion led by: Alberto Pugliese, MD

nPOD-Autoimmunity Group Slice RFA Webinar

DATE: Wednesday, April 24th, 2019 Download Meeting Recording: Dropbox link to view meeting recording  Discussion led by: Alberto Pugliese, MD and Sally Kent, PhD  

nPOD-Virus Group Slice RFA Webinar

DATE: Tuesday, April 23rd, 2019 Download Meeting Recording: Dropbox link to view meeting recording  Discussion led by: Alberto Pugliese, MD and Sarah Richardson, PhD  

Autoimmunity Group Webinar

DATE: Thursday, April 25th, 2019 Download Meeting Recording: Dropbox link to view meeting recording   Discussion led by: Alberto Pugliese, MD and Sally Kent, PhD

10th Annual OPO Workshop

nPOD 10th Annual OPO Workshop This year’s workshop will be held in beautiful St. Pete Beach, Florida on October 22-24, at the TradeWinds Islands Grand Resort. Registration will be free! Continuing education credits, hotel room, meals and entertainment are complimentary. Attendees are only responsible for their travel to and from the workshop. nPOD relies on your research organ referrals […]