The individual heterogeneities of Type 1 diabetic pancreas could hinder a successful investigation and classification of the underlying mechanisms in diabetic disorders. This indicates the necessity of in situ analysis of tissue sections by novel imaging mass spectrometry technology, which enables the spatial investigation of protein profiles from an examined tissue section, thus allowing to relate and to visualize changes in tissue histology to changes in the proteomic signature of that tissue. Through this systemic technique we will be able to understand the pathological cause of the changes during early stages of Type 1 diabetes, which could give us more insight into pinpointing preventive strategies for this disease.