In collaboration with the New York Stem Cell Foundation (NYSCF, see letter of collaboration), we will attempt de-differentiation of cryopreserved splenocytes to iPSC: this has not been attempted before to our knowledge. If successful, these iPSC may be differentiated into sc-islets and serve as an isogenic sources of sc-islets for interactions with the islet-derived T cell lines the Kent lab has generated and banked. If successful, we will submit a full addendum to this project for the study of the interaction of isogenic iPSC-derived sc-islets and autologous islet-derived T cells. This technology may be used by other investigators in their examination of the isogenic interaction of sc-islets and immune cells.
