Generated by All in One SEO v5.0.0.1, this is an llms.txt file, used by LLMs to index the site.

# nPOD

Network for Pancreatic Organ Donors with Diabetes

## Sitemaps

- [XML Sitemap](https://npod.org/sitemap.xml): Contains all public & indexable URLs for this website.

## Posts

- [Congruence of lab versus histopathologic findings of acute pancreatitis in the nPOD consortium](https://npod.org/current-npod-projects/congruence-of-lab-versus-histopathologic-findings-of-acute-pancreatitis-in-the-npod-consortium/) - Acute pancreatitis is the cause of more than 200,000 hospital admissions in the United States each year (1). Acute pancreatitis presents very broadly, hence diagnosis can be difficult and requires confirmatory tests. Serum amylase and lipase levels have been established as markers for disease. However, they lack adequate specificity and sensitivity which might make their
- [Stellate cells in type 1 diabetes research (STELLAR)](https://npod.org/current-npod-projects/stellate-cells-in-type-1-diabetes-research-stellar/) - The loss of insulin-producing β-cells is central to the pathogenesis of type 1 diabetes (T1D). More recently, the contribution of the exocrine pancreatic cellular components is becoming evident in determining the β-cells well-being. Other pancreatic cell types could be acting by reducing β-cell resistance to the autoimmune attack or inducing a stressful condition that will
- [Redoxins in the pathogenesis of type 1 diabetes](https://npod.org/current-npod-projects/redoxins-in-the-pathogenesis-of-type-1-diabetes/) - We plan to quantify redoxin expression levels and the frequency and type of redoxin expressing pancreatic cells by imaging procedures established in our group. For this purpose we propose a pilot study with samples from #3 cases ideally with rapid (pre-type 1 or type 1 diabetes) or more chronic (type 2 diabetes) ongoing beta cell
- [Project 1: Human Pancreatic Slice-Based Studies of Regeneration and Exocrine–Endocrine Cross-Talk (In Vitro)](https://npod.org/current-npod-projects/project-1-human-pancreatic-slice-based-studies-of-regeneration-and-exocrine-endocrine-cross-talk-in-vitro/) - The different cellular compartments of the pancreas have a high degree of plasticity, which, if harnessed, could have important therapeutic implications for beta-cell regeneration in type 1 and type 2 diabetes. This proposal focuses on high-resolution characterization of ductal progenitor-like cells (P2RY1+/ALK3+) and exocrine-endocrine communication in live human pancreatic slices. We will use dynamic single-cell
- [Modeling Autoimmune β-Cell Destruction in Human Pancreatic Slices, SliceChip, and Slice-in-ACE Platforms](https://npod.org/current-npod-projects/modeling-autoimmune-β-cell-destruction-in-human-pancreatic-slices-slicechip-and-slice-in-ace-platforms/) - Autoimmune β-cell loss in T1D occurs in a three-dimensional tissue context that current cell culture systems fail to replicate. HPSs preserve native islet-exocrine relationships and tissue architecture, providing a unique human platform to observe immune damage in real time. This revised application focuses on building controlled models of autoimmune β-cell injury, not on requesting rare
- [Human Pancreatic Slice-in-ACE Models for Regenerative Studies](https://npod.org/current-npod-projects/human-pancreatic-slice-in-ace-models-for-regenerative-studies/) - The developmental plasticity of the pancreas has been extensively studied. Leaving aside the ongoing debate about the relative contribution of neogenesis and endocrine cell self-duplication to islet regeneration in mice, the notion that progenitor cells can regenerate islets has been recently substantiated in human models. That this potential is intrinsic is evidenced by the observation
- [Integrating functional and transcriptomic measurements in pancreatic islets with spatial resolution](https://npod.org/current-npod-projects/integrating-functional-and-transcriptomic-measurements-in-pancreatic-islets-with-spatial-resolution/) - Type 1 diabetes mellitus (T1D) is a complex autoimmune condition which is globally raising, it is influenced by genetic predisposition and environmental factors such as dietary changes and potential exposure to viruses or toxins. Single-cell methods have recently shown that endocrine islet cells are molecularly and functionally heterogeneous, and that cell-to-cell interactions within islets are
- [Discovery of novel autoantibodies for accurate diagnosis of T1D](https://npod.org/current-npod-projects/discovery-of-novel-autoantibodies-for-accurate-diagnosis-of-t1d/) - Type 1 diabetes (T1D) is typically diagnosed when patients lose their ability to produce sufficient amounts of insulin. However, this is merely the final stage in a long process which involves the gradual loss of beta cell mass due to an autoimmune attack which specifically targets insulin producing beta cells in the pancreatic islets. Recently,
- [Exploring miRNAs heterogeneity within pancreatic islet cells’ subpopulations in the context of T1D](https://npod.org/current-npod-projects/exploring-mirnas-heterogeneity-within-pancreatic-islet-cells-subpopulations-in-the-context-of-t1d/) - Type 1 diabetes (T1D) happens when the immune system attacks and destroys the insulin-producing beta-cells in the pancreas, which are contained in the islets of Largenhans. Research has shown that beta-cells within islets are not all identical: some differ from others in the genes they use and in how well they work. We think that
- [Dysregulation of molecular and cellular pathways in the pancreas during type 1 diabetes](https://npod.org/current-npod-projects/dysregulation-of-molecular-and-cellular-pathways-in-the-pancreas-during-type-1-diabetes/) - Type 1 diabetes (T1D) is an autoimmune disease in which the insulin-producing β cells in the pancreas are selectively destroyed. Both genetic and environmental factors, such as viral infections, are implicated in the disease pathogenesis but how these factors contribute to the selective destruction of β cells at the cellular level remains unclear. Accumulating data
- [New Faces at nPOD](https://npod.org/news-events/new-faces-at-npod/) - uzanne Ball, RN, MHS, nPOD Director Suzy brings twenty years of experience in the field of donation and transplantation. She has worked as the Director for both organ and tissue organizations. She has also served as two-term Chair of the National Donor Family Executive Council for the National Kidney Foundation. Suzy is a Registered Nurse,
- [De-differentiation of cryopreserved splenocytes to iPSC as an isogenic resource for differentiation to sc-islets. Addendum: De-differentiation of cryopreserved islet-derived T cell lines to iPSC as a resource for differentiation to sc-islets](https://npod.org/current-npod-projects/de-differentiation-of-cryopreserved-splenocytes-to-ipsc-as-an-isogenic-resource-for-differentiation-to-sc-islets/) - In collaboration with the New York Stem Cell Foundation (NYSCF, see letter of collaboration), we will attempt de-differentiation of cryopreserved splenocytes to iPSC: this has not been attempted before to our knowledge. If successful, these iPSC may be differentiated into sc-islets and serve as an isogenic sources of sc-islets for interactions with the islet-derived T
- [Age-related heterogeneity of type 1 diabetes: a multi-omics, spatially resolved, exploration of the human pancreas at disease onset](https://npod.org/current-npod-projects/age-related-heterogeneity-of-type-1-diabetes-a-multi-omics-spatially-resolved-exploration-of-the-human-pancreas-at-disease-onset/) - Type 1 diabetes (T1D) is a chronic autoimmune disease long thought to primarily affect children and adolescents. However, recent data suggest that nearly 70 percent of new T1D cases occur in adults. Striking differences in genetic, metabolic, and immune features as well as in diabetes management have been described between childhood- and adult-onset T1D, but
- [Small endocrine objects – the key to endocrine mass expansion?](https://npod.org/current-npod-projects/small-endocrine-objects-the-key-to-endocrine-mass-expansion/) - Advances in 2D and 3D imaging techniques and technologies have enabled deeper interrogation of rare pancreas bioresources, highlighting significant gaps in our knowledge, with implications for the understanding of normal development and pancreatic disease. 3D volumetric imaging of the adult pancreas has revealed that 40-50% of the total endocrine objects within the organ consist of
- [The role of beta cell lysosomal dysfunction in diabetes autoimmunity](https://npod.org/current-npod-projects/the-role-of-beta-cell-lysosomal-dysfunction-in-diabetes-autoimmunity/) - As alterations in autophagic flux may contribute to T1D, we are seeking to determine if particular lysosomal components of beta cell autophagy participate in or dysregulate this essential process. The focus of this addendum is on the enzyme cathepsin H as specific genetic variants in this gene have been identified as T1D-associated SNPs. Through these
- [Stromal sentinel cells are activated in T1D and organize tertiary lymphoid structures](https://npod.org/current-npod-projects/stromal-sentinel-cells-are-activated-in-t1d-and-organize-tertiary-lymphoid-structures/) - Preliminary data indicates that stromal cells within the vicinity of islets acquire properties that can organize ectopic lymph node like structures (known as tertiary lymphoid structures or TLS) in NOD mice. These properties include differentiating of stromal cells into specific cell types that can secrete chemokines to organize infiltration of T and B cells and
- [The role of the pancreas microenvironment in type 1 diabetes progression](https://npod.org/current-npod-projects/the-role-of-the-pancreas-microenvironment-in-type-1-diabetes-progression/) - The pancreatic islet is surrounded by an extracellular matrix (ECM) composed of laminin, collagen IV, and proteoglycans, that support islet function and viability. Type 1 diabetes (T1D) is characterized by the immunemediated loss of insulin-producing β-cells in the islet. Islet infiltration by activated immune cells results in the degradation of the peri-islet ECM eliminating critical
- [Role of pericytes in innate immune responses in pancreatic islets](https://npod.org/current-npod-projects/role-of-pericytes-in-innate-immune-responses-in-pancreatic-islets/) - Vascular defects are present in islets during stages 1 and 2 of type 1 diabetes, when symptomatic disease has not yet developed. However, the cellular and molecular mechanisms underlying vascular dysfunction and they link with autoimmunity have not been identified. This knowledge is crucial to understand T1D pathogenesis. In this proposal we plan to start
- [Role Integrated Stress Response Pathways in Health and Disease](https://npod.org/current-npod-projects/role-integrated-stress-response-pathways-in-health-and-disease/) - Type 1 diabetes (T1D) development is characterized by insulitis, resulting in β-cell stress and progressive β-cell dysfunction and death. However, recent evidence suggest the role of pancreatic β cells in amplifying this immune response through the presentation of immunogenic peptides and secretion of cytokines and chemokines, indicating β cells may be complicit during the development
- [Enterovirus infection and pancreatic macrophage phenotypes in type 1 diabetes](https://npod.org/current-npod-projects/enterovirus-infection-and-pancreatic-macrophage-phenotypes-in-type-1-diabetes/) - Type 1 diabetes (T1D) is a chronic autoimmune disease targeting pancreatic beta cells. The incidence of T1D has been increasing, a trend inexplicable by genetic predisposition alone, indicating that environmental factors such as viruses play important roles in triggering the disease. Decades of research have provided epidemiological evidence for an association between T1D and enteroviruses,
- [Generation of a Comprehensive Atlas of the Human Pancreas in Type 2 Diabetes](https://npod.org/current-npod-projects/generation-of-a-comprehensive-atlas-of-the-human-pancreas-in-type-2-diabetes/) - Type 2 diabetes (T2D) is one of the most common chronic conditions worldwide, affecting hundreds of millions of people. It develops when the pancreas cannot produce enough insulin and/or when the body no longer responds to insulin effectively. While it is clear that insulin-producing β-cells fail in T2D, the reasons for this failure remain poorly
- [Characterizing the association of PD-L1 splice variants in type 1 diabetes](https://npod.org/current-npod-projects/characterizing-the-association-of-pd-l1-splice-variants-in-type-1-diabetes/) - Type 1 diabetes (T1D) exhibits significant heterogeneity in disease progression, driven in part by variable immune-mediated destruction of insulin-producing β cells. This project focuses on Programmed Death-Ligand 1 (PD-L1), a critical immune checkpoint protein that protects β cells by suppressing autoimmune responses via interaction with PD-1 on immune cells [1]. β cells also release PD-L1
- [Oxidized Insulin as a Biomarker for Type 1 Diabetes Pathogenesis](https://npod.org/current-npod-projects/oxidized-insulin-as-a-biomarker-for-type-1-diabetes-pathogenesis/) - Reactive oxidants are generated during the metabolism of glucose and serve as important signaling messengers to trigger insulin secretion and beta cell expansion in response to elevated levels of glucose. However, chronic exposure to oxidants under hyperglycemic conditions causes chronic oxidative stress which results in cellular damage, impaired glucose-stimulated insulin secretion, and, eventually, cell death.
- [Role of pancreas-associated myeloid cells in homeostasis and T1D](https://npod.org/current-npod-projects/role-of-pancreas-associated-myeloid-cells-in-homeostasis-and-t1d/) - This project aims to uncover how macrophage dysregulation promotes autoimmune β-cell destruction. Our lab has identified distinct pancreatic macrophage subtypes that mediate localized immune control by regulating T cell activity. Aim 1 will use single cell sequencing to comprehensively profile pancreatic macrophage subsets and their communication with T cells during immune homeostasis and in Type
- [Harnessing the local cholinergic anti-inflammatory reflex in the pancreatic islet to delay or prevent type 1 diabetes](https://npod.org/current-npod-projects/harnessing-the-local-cholinergic-anti-inflammatory-reflex-in-the-pancreatic-islet-to-delay-or-prevent-type-1-diabetes/) - Type 1 Diabetes (T1D) is characterized by autoimmune destruction of insulin-producing beta cells within the pancreatic islets. Resident macrophages in the islets play a key role in maintaining tissue homeostasis but can also promote inflammation. The cholinergic anti-inflammatory reflex, known to suppress inflammation, may influence islet macrophages, but direct cholinergic innervation has not been established.
- [Investigating the role of iron metabolism in beta cell function and the immunopathology of type 1 diabetes](https://npod.org/current-npod-projects/investigating-the-role-of-iron-metabolism-in-beta-cell-function-and-the-immunopathology-of-type-1-diabetes/) - Insulin release from beta cells depends on ATP energy generated by mitochondrial. The electron transport chain is the primary group of proteins that make ATP energy and they require iron to do so. Consequently, iron is essential for insulin release, and iron deficiency inhibits beta cell function. Conversely, excess cellular iron leads to an increase
- [Live human pancreas tissue slices as a platform for investigating the immunopathological processes of type 1 diabetes](https://npod.org/current-npod-projects/live-human-pancreas-tissue-slices-as-a-platform-for-investigating-the-immunopathological-processes-of-type-1-diabetes/) - Type 1 diabetes (T1D) is characterized by the loss of insulin producing β cells precipitated by infiltration of the pancreas by autoimmune T lymphocytes. We have shown that there is a parallel loss of function in the mass of β cells still remaining when T1D is diagnosed. This project aims to better understand how and
- [Investigation of Myeloid CADM1 during T1D](https://npod.org/current-npod-projects/investigation-of-myeloid-cadm1-during-t1d/) - Type 1 diabetes (T1D) is characterized by the hyperglycemia resulting from the autoimmune destruction of the insulin-expressing β-cells of the pancreas. As the prevalence of the disease continues to increase worldwide, it remains imperative to identify therapeutic strategies that can (1) preserve pancreatic beta-cell mass and function and (2) prevent the immune response that initiates
- [Pancreatic Beta-Cells, Oxidative Stress, and Gestational Diabetes](https://npod.org/current-npod-projects/pancreatic-beta-cells-oxidative-stress-and-gestational-diabetes/) - The late stages of mammalian pregnancy are accompanied by a mild increase in insulin resistance, likely due to enhanced glucose demand for the growing fetus. Therefore, as an adaptive process to maintain normal blood glucose levels during pregnancy, maternal insulin-producing cell (β-cell) mass expands, leading to increased insulin release. Defects in functional β-cell adaptive expansion
- [Proinsulin biosynthesis and (mis)folding in type 1 diabetes](https://npod.org/current-npod-projects/proinsulin-biosynthesis-and-misfolding-in-type-1-diabetes/) - A well-recognized feature of pancreatic ß-cells is their limited expression of endogenous antioxidants. From recent support by nPOD and HIRN/CBDS, my lab made the entirely novel observation, using metabolic labeling in pancreatic slices derived from 5 T1D donors, that disulfide-bond formation (i.e., folding) of newly-synthesized (radiolabeled) proinsulin is dramatically impaired with a surprisingly large fraction
- [Understanding common tissue tolerance mechanisms in autoimmunity](https://npod.org/current-npod-projects/understanding-common-tissue-tolerance-mechanisms-in-autoimmunity/) - A curious feature of type I diabetes (T1D) is that, while the proteins that the immune system targets are broadly expressed in beta cells, inflammation of insulin-producing cells (insulitis) is patchy and damage develops gradually. In stage 1 or 2 T1D, prior to the onset of clinically evidence disease, the majority of insulin-producing cells are
- [Dynamic Survey of T Cell Repertoire Targeting Pancreatic Beta Cells](https://npod.org/current-npod-projects/jdrf-44-2008-913-dynamic-survey-of-t-cell-repertoire-targeting-pancreatic-beta-cells/) - Our project aims to define islet-specific T cell receptor (TCR) sequence repertoires directly from pancreas to pursue two goals; 1) to identify antigens targeted by T cells contributing to the development of type 1 diabetes (T1D); and 2) to pursue the potential of TCR sequences to be used as biomarker. We hypothesize that common antigens
- [Immune receptor repertoires associated with type 1 diabetes progression](https://npod.org/current-npod-projects/immune-receptor-repertoires-associated-with-type-1-diabetes-progression/) - Immune receptors, including T cell receptors (TCRs) on T cells and B cell receptors (BCRs) on B cells, are central to immune responses, mediating antigen recognition and tissue specificity. Characterizing the immune receptor repertoires during type 1 diabetes (T1D) progression provides key insights into the T and B cell populations contributing to disease pathogenesis. The
- [Antigen discovery for regulatory T cells in T1D](https://npod.org/current-npod-projects/antigen-discovery-for-regulatory-t-cells-in-t1d/) - The Brusko Lab is generally interested in the cellular immune response involved in the disease process leading to type 1 diabetes (T1D). A strong genetic association between T1D and the MHC class II gene region of the adaptive immune system has implicated coordinated T and B cell responses as a central mediator of the disease
- [T cell autoreactivity, phenotype and function directly from islets of donors with recent onset type 1 diabetes (T1D) and from donors with islet-associated autoantibodies, but without clinical disease](https://npod.org/current-npod-projects/t-cell-autoreactivity-phenotype-and-function-directly-from-islets-of-donors-with-recent-onset-type-1-diabetes-t1d-and-from-donors-with-islet-associated-autoantibodies-but-without-clinical-disease/) - The goal of this project is to further our understanding of the CD4 T cells and CD8 T cells that infiltrate the islets in autoantibody+ donors at risk for development of type 1 diabetes (T1D) and from donors with recent onset T1D. We will hand pick islets from digests of live pancreas slices from the
- [Evaluation of the islet-immune interface in Cystic Fibrosis-related Diabetes](https://npod.org/current-npod-projects/evaluation-of-the-islet-immune-interface-in-cystic-fibrosis-related-diabetes/) - Cystic fibrosis (CF)-related diabetes (CFRD) is present in upwards of 20% of adolescents and 40-50% of adults with CF and is associated with significant glycemic variability, poor glycemic control, and unpredictable hypoglycemia. CFRD is increasing in prevalence as new disease-modifying therapies have led to a significant increase in life expectancy in those with CF. While
- [Defining the link between Islet prohormone accumulation and secretion in type 1 diabetes](https://npod.org/current-npod-projects/defining-the-link-between-islet-prohormone-accumulation-and-secretion-in-type-1-diabetes/) - An improved understanding of the pathophysiologic mechanisms contributing to insulin deficiency in type 1 diabetes (T1D) is crucial to optimize approaches to disease prevention and treatment. Multiple groups have described increases in beta cell prohormones in individuals at different stages of T1D development, both at the level of the islet and in circulation. These findings
- [Single Islet Functional Heterogeneity from control donors, auto antibody+ donors, and donors with recent onset type 1 diabetes (T1D)](https://npod.org/current-npod-projects/single-islet-functional-heterogeneity-from-control-donors-auto-antibody-donors-and-donors-with-recent-onset-type-1-diabetes-t1d/) - In T1D, pancreatic islets are infiltrated by lymphocytes (so called “insulitis”) and those T cells appear to drive β-cell dysfunction, and death. However, it has been long observed that not all islets within a T1D donor’s pancreas are infiltrated; even those in close proximity such that some islets have many infiltrating T cells while others
- [Functional alpha cell heterogeneity](https://npod.org/current-npod-projects/functional-alpha-cell-heterogeneity/) - In type 1 diabetes (T1D), the destruction of insulin-producing beta cells is well known. But another emerging issue— not well understood—is the dysfunction of alpha cells. These cells produce glucagon, a hormone that helps prevent blood sugar from dropping too low. In people with T1D, alpha cells often fail to respond properly when blood sugar
- [Assessing T1D islet dysfunction: β-β cell connectivity defects, T-cell induced islet dysfunction, and cystic fibrosis-related diabetes (CFRD)](https://npod.org/current-npod-projects/assessing-t1d-islet-dysfunction-β-β-cell-connectivity-defects-t-cell-induced-islet-dysfunction-and-cystic-fibrosis-related-diabetes-cfrd/) - The availability of pancreas slices from nPOD from T1D and normal control donors has provided a huge thrust in the study of T1D islet biology as it requires the inflamed islet to be intact within a pancreas slice for accurate assessment. We have developed capabilities to culture these pancreas slices for ~10 days allowing virus
- [Connection between alpha cell function and T-cell antigen specificity in type 1 diabetes](https://npod.org/current-npod-projects/connection-between-alpha-cell-function-and-t-cell-antigen-specificity-in-type-1-diabetes/) - Given that in Aab+ donors alpha cells become dysfunctional before donor’s blood glucose levels are impaired, it is important to differentiate whether a) alpha cells become dysfunctional first, which attracts and activates immune cells, or b) vice versa. In pre-T1D the first phase insulin secretion is dampened. We and others have shown that this phase
- [Uridine in the pathogenesis of T1D](https://npod.org/current-npod-projects/uridine-in-the-pathogenesis-of-t1d/) - Blood uridine levels are elevated in T1D. However, its significance to the disease pathogenesis remains largely unexplored. Our pilot studies indicate uridine regulates glucose-stimulated insulin secretion (GSIS) in mouse and human islets. We will use human pancreas tissue sections and live pancreas slices to study how chronic elevation in blood uridine might affect beta cell
- [Expression of pancreatic Notch1 in type 1 diabetes](https://npod.org/current-npod-projects/expression-of-pancreatic-notch1-in-type-1-diabetes/) - Pancreatic tissue is composed of two dominant cell types: small clusters of insulin producing cells called the islets of Langerhans and exocrine cells in the surrounding stroma. The endocrine and exocrine pancreatic tissues are fundamentally different in terms of anatomical organization, basic function and pathological conditions, and they are consequently believed to be largely independent
- [Investigation of the sexual dimorphic phenotype of complosome expressing medullary thymic epithelial cells, and thymic B cells in type 1 diabetes](https://npod.org/current-npod-projects/investigation-of-the-sexual-dimorphic-phenotype-of-complosome-expressing-medullary-thymic-epithelial-cells-and-thymic-b-cells-in-type-1-diabetes/) - Quality control-the removal of immune cells that have the ability to attack our own tissues-is a central tenet of the immune system. In type 1 diabetes (T1D), impaired quality control in the thymus, leads to release of beta cell-reactive immune cells (called T cells) into the blood; such cells facilitate destruction of insulin-producing beta cells
- [Determination of RFX3 and RFX6 expression in human fetal, neonatal, childhood and young adult pancreas](https://npod.org/current-npod-projects/determination-of-rfx3-and-rfx6-expression-in-human-fetal-neonatal-childhood-and-young-adult-pancreas/) - The human pancreas is a vital organ responsible for both endocrine and exocrine functions, playing a crucial role in glucose metabolism and digestion. It originates from the foregut endoderm, with the dorsal and ventral buds forming and eventually fusing to develop into a mature pancreas. Key stages of pancreas development include specification, branching morphogenesis, and
- [Investigating the role of Hippo pathway in Type 1 Diabetes](https://npod.org/current-npod-projects/investigating-the-role-of-hippo-pathway-in-type-1-diabetes/) - The prevalence of type 1 diabetes (T1D) is on the rise, posing a significant burden on healthcare systems. T1D results from the immune system mistakenly attacking insulin-producing pancreatic β-cells, necessitating lifelong insulin treatment and continuous monitoring of blood sugar levels. The complex inflammatory nature of T1D, coupled with substantial gaps in our understanding of the
- [The immunogenicity of abnormal catabolites of insulin metabolism](https://npod.org/current-npod-projects/the-immunogenicity-of-abnormal-catabolites-of-insulin-metabolism/) - We are searching for abnormal products of insulin metabolism that appear in beta cell as a result of the increased demands for insulin. These abnormal catabolites in NOD mice select for very unique CD4 T cells that initiate this chronic autoimmune reaction. The plans are to search for these abnormal products in islets from early
- [nPOD Data Portal](https://npod.org/npod-webinars/npod-data-portal/) - DATE: Friday, September 6th, 2024 TIME: 11:00AM EST Meeting Recording: Dropbox link to view recording Discussion led by: Helmut Hiller, MS & Maria Beery, MS In this webinar, we guided you through the nPOD Data Portal website, and showed you how to navigate, access, and download our new genetic data, including SNPs-genes and immunophenotyping data.
- [Do enteroviruses trigger diabetes progression?](https://npod.org/current-npod-projects/do-enteroviruses-trigger-diabetes-progression/) - Identification of the driver of autoimmunity and early intervention before established disease is urgently needed. As in many autoimmune diseases, viral infections have been associated with T1D, recently confirmed by large and highly sensitive screenings using well-characterized material from autopsy and biopsy. We looked closer into the pancreases from organ donors with early T1D-associated autoimmunity
- [The Heterogeneity of Type 1 Diabetes: An Islets Story](https://npod.org/current-npod-projects/the-heterogeneity-of-type-1-diabetes-an-islets-story/) - When a person is diagnosed with Type 1 diabetes (T1D), the amount of beta-cell mass each individual loses is highly variable despite their clinical need for insulin therapy. Attempts to detect robust markers in the blood that precisely describe disease activity in the islets are ongoing, but without a full understanding of the disease course
- [The role of human ductal cells in propagating pathogenesis of type 1 diabetes](https://npod.org/current-npod-projects/the-role-of-human-ductal-cells-in-propagating-pathogenesis-of-type-1-diabetes/) - Type 1 diabetes (T1D) is a pancreatic disease marked by the autoimmune destruction of insulin-secreting beta cells within the endocrine compartment, leading to chronic hyperglycemia and long-term complications. Despite extensive studies into the autoimmunity that targets beta cells, a complete understanding of the pathogenesis of T1D remains unclear. A recent study by Fasolino et al.
- [T cell autoreactivity in pancreatic draining lymph nodes (pLN) of donors with cystic fibrosis-related diabetes (CFRD) and type 1 diabetes (T1D)](https://npod.org/current-npod-projects/t-cell-autoreactivity-in-pancreatic-draining-lymph-nodes-pln-of-donors-with-cystic-fibrosis-related-diabetes-cfrd-and-type-1-diabetes-t1d/) - Many individuals with cystic fibrosis (CF) develop diabetes or CF-related diabetes (CFRD).Some published data indicates that CFRD shares autoimmune phenotypes in common with type 1 diabetes (T1D). These include a higher prevalence of islet autoantibodies for CFRD patients than the general population and signs of broad immune dysregulation (including a Th17 signature). Notably, in autoantibody-positive
- [Leveraging heterogeneity to identify markers of resilient β-cells in T1D](https://npod.org/current-npod-projects/leveraging-heterogeneity-to-identify-markers-of-resilient-β-cells-in-t1d/) - We aim to identify factors contributing to the destruction ofβ-cells in diabetes. In this project, we took advantage of publicly available bulk and single cell gene expression datasets from human islets of healthy and diabetic donors(T1D and T2D). We used deep transfer learning platform DEGAS1 combinedwith this data to identify subsets of β-cells within human
- [Immunoregulation of type 1 diabetes](https://npod.org/current-npod-projects/immunoregulation-of-type-1-diabetes/) - Type 1 Diabetes (T1D) is a disease that results from the autoimmune destruction of insulin-producing beta cells in the pancreas. The current treatment for T1D does not cure the disease or prevent the possibility of other serious complications. The mechanism that leads to the loss of immune tolerance still remains unclear; scientists believe that both
- [Human pancreatic tissue proteome of type 1 diabetes](https://npod.org/current-npod-projects/human-pancreatic-tissue-proteome-of-type-1-diabetes/) - The goal of this project is to comprehensively profile the proteins expressed in human pancreas within both the islets and their surrounding environments, and to obtain relative expression levels of pancreatic proteins from different organ donor groups using mass spectrometry based bottom-up and top-down proteomics technologies. Snap-frozen pancreatic tissue sections from organ donor groups with
- [Humoral immunity in type 1 diabetes](https://npod.org/current-npod-projects/humoral-immunity-in-type-1-diabetes/) - Our project entitled humoral immunity investigates several serological aspects of Type 1 Diabetes (T1D). Our group also serves as the ELISA autoantibody core wherein we oversee the screening laboratories and together with the University of Colorado maintain quality assurance and control for this program. However we also conduct research into additional aspects of autoantibodies from
- [Role of mucosal epithelium in autoreactivity](https://npod.org/current-npod-projects/role-of-mucosal-epithelium-in-autoreactivity/) - One of the major questions we still don’t have an answer to is why some people who are genetically predisposed for development of T1D don’t progress and why others do. In addition, why there are different timings for the progression of this disease (i.e. why are some faster than others). Our thoughts are that these
- [Characterization of Mesencymal Stem Cells in T1D](https://npod.org/current-npod-projects/characterization-of-mesencymal-stem-cells-in-t1d/) - The development of MCS-based therapies for autoimmune diseases, including T1D, is a new field with a lot of promise, but also a lot of uncertainty. While successful treatment of disease allogeneic MSCs has been achieved, some MSC treatment failure has also been reported. The significance of this proposal is that it will fill a significant
- [The role of IL-15 and IL-15Ra in the pathogenesis of type 1 diabetes](https://npod.org/current-npod-projects/the-role-of-il-15-and-il-15ra-in-the-pathogenesis-of-type-1-diabetes/) - Interleukin-15 (IL-15) is a pro-inflammatory cytokine that promotes the activation and maintenance of natural killer (NK) and CD8 (+) T-effector memory (T-EM) cells. In patients with type 1 diabetes, elevated serum levels of IL-15 have been reported. Using an assay developed in the lab, we demonstrated that the serum sIL-15Rα levels were significantly higher in
- [Mass Spectrometry Based Digital Histology nPOD (Berlin BCRT)](https://npod.org/current-npod-projects/mass-spectrometry-based-digital-histology-npod-berlin-bcrt/) - The individual heterogeneities of Type 1 diabetic pancreas could hinder a successful investigation and classification of the underlying mechanisms in diabetic disorders. This indicates the necessity of in situ analysis of tissue sections by novel imaging mass spectrometry technology, which enables the spatial investigation of protein profiles from an examined tissue section, thus allowing to
- [In situ detection of pro-inflammatory cytokines within pancreatic islets from diabetic subjects](https://npod.org/current-npod-projects/in-situ-detection-of-pro-inflammatory-cytokines-within-pancreatic-islets-from-diabetic-subjects/) - In Type 1 Diabetes (T1D), the complex interplay of immune cells shifts the balance in favor of the development of lymphocytes, that attack structures of our own body: autoimmunity. These attacks resulting in an inflammation in the pancreas and, eventually, to the destruction of insulin producing beta cells. Cytokines are small proteins that are released
- [Pathological changes associated with chronic RelB activation in T1D](https://npod.org/current-npod-projects/pathological-changes-associated-with-chronic-relb-activation-in-t1d/) - Dendritic cells (DCs) play a crucial role in establishing and maintaining the balance between immunity to pathogens and tolerance to self. Whether DCs evoke T cell activation or tolerance in response to antigen presentation is determined by the physiological context in which they differentiate and mature. DC differentiation and inflammatory signalling is abnormal in type
- [Detection of enteroviruses in lymphoid tissue of donors with T1D of short duration and attempts to identify the infected cell type(s)](https://npod.org/current-npod-projects/detection-of-enteroviruses-in-lymphoid-tissue-of-donors-with-t1d-of-short-duration-and-attempts-to-identify-the-infected-cell-types/) - If type 1 diabetes (T1D) is caused/triggered by a viral infection in select genetic backgrounds - and Enteroviruses (EVs) are felt as major culprits - investigators are expecting to find EVs into pancreatic Langerhans islets and also in association with spleen, lymph nodes, peripheral blood leukocytes. Infection of lymphoid cells, in fact, is common in
- [Enteroviral infection and T1D](https://npod.org/current-npod-projects/enteroviral-infection-and-t1d/) - Despite convincing evidence linking human enteroviruses (HEV) with type 1 diabetes (T1D) onset, it is not known whether certain HEV are the ones which are most commonly associated with T1D onset or if T1D onset is associated with any HEV serotype.
- [The patterns of NADPH oxidase-1 expression in the course of human diabetes](https://npod.org/current-npod-projects/the-patterns-of-nadph-oxidase-1-expression-in-the-course-of-human-diabetes/) - Increased levels of reactive oxygen species (ROS) lead to dysfunction and subsequent loss of insulin-producing beta cells in pancreatic islets. Several lines of evidence support the importance of increased NADPH oxidase (NOX) activity as one of the contributing factors to elevated levels of ROS in beta cells. NOX-1 activity has been established as one of
- [Characterization of new cell markers associated with beta cell failure in diabetes](https://npod.org/current-npod-projects/characterization-of-new-cell-markers-associated-with-beta-cell-failure-in-diabetes/) - The percentage of reduction of beta cell mass in patients with Type 1 diabetes (T1D) of recent onset cannot fully explain the reduction of endogenous insulin production that leads to hyperglycemia. In addition, functional defects of the beta cells may prevail from reduction of beta cell mass during Type 2 diabetes. In boty situation chronic
- [Using Emerging Techniques to Identify Novel Islet Signatures in Type 1 and Type 2 Diabetes](https://npod.org/current-npod-projects/using-emerging-techniques-to-identify-novel-islet-signatures-in-type-1-and-type-2-diabetes/) - Though the pancreatic islets are known to play critical roles in both Type 1 diabetes (T1D) and Type 2 diabetes (T2D), the distinct mechanisms are poorly understood. Recent findings demonstrate that chemical heterogeneity exists within the classic islet endocrine cell types, even in the normal/healthy pancreas setting. For example, there are subtypes of beta cells
- [Peri-capsular basement membrane degradation during leukocyte penetration Into the pancreatic islet during development of human type 1 diabetes and tertiary lymphoid organs in human T1D](https://npod.org/current-npod-projects/peri-capsular-basement-membrane-degradation-during-leukocyte-penetration-into-the-pancreatic-islet-during-development-of-human-type-1-diabetes/) - Several steps are crucial for induction of type 1 diabetes (T1D); the first is extravasation of CD4+ T lymphocytes from blood vessels into the pancreatic tissue, the second is penetration of the peri-islet basement membrane (BM) surrounding the β-islets, and third the β-cell destruction which leads to appearance of disease symptoms. BMs act to separate
- [Development of a platform for ex-vivo functional and 3D morphological assessment of islet physiology in nPOD human pancreata, also in response to viral infections associated with type 1 diabetes pathogenesis](https://npod.org/current-npod-projects/4289/) - Impairment and destruction of beta cells by an autoimmune attack leads to the onset of hyperglycemia in type 1 diabetes (T1D). However, knowledge on changes in islet cell physiology leading to dysfunction, failure and death of the cells is incomplete. This is partially due to technical limitations which do not allow the study of islet
- [Targeting AID expressing B-lymphocytes as possible clinically applicable T1D intervention](https://npod.org/current-npod-projects/targeting-aid-expressing-b-lymphocytes-as-possible-clinically-applicable-t1d-intervention/) - Diabetogenic B-lymphocyte activity may be enhanced by SHM induced increases in Ig antigen affinity. -SHM requires AID induced double strand DNA breaks repaired by RAD51 complex proteins. -AID is expressed at higher levels in B-lymphocytes from NOD mice than control strains -A small RAD51 blocking molecule specifically induces apoptosis of AID expressing B-lymphocytes from both
- [Analysis and comparison of IL-18, IL-18R, CCL19 and CCL21 expression in T1D, T2D, autoantibody positive and control samples](https://npod.org/current-npod-projects/to-identify-the-role-of-il-18r-cd8-t-cell-subsets-in-islet-destruction/) - Type 1 diabetes (T1D) has devastating effects on the health and quality of life of those affected. Several studies have associated the proinflammatory cytokine IL‐18 with an increased risk of T1D. Significantly higher IL-18 cytokine levels are seen in individuals at high risk for developing disease, increased serum levels of IL‐18 have been observed in
- [Pancreatic antibacterial responses in T1D](https://npod.org/current-npod-projects/pancreatic-antibacterial-responses-in-t1d/) - The etiology of autoimmune diabetes is not known. However, the disease is accompanied by varying amounts of immune activation and inflammation. Although genetics clearly plays a role in determining who gets the disease, we do not know the instigating events. There has been a lot of investigation into the potential role of virus infections in
- [In situ gene profiling and identification of T1D disease mechanisms](https://npod.org/current-npod-projects/in-situ-gene-profiling-and-identification-of-t1d-disease-mechanisms/) - This CBDS program will establish an in situ RASL-Seq Islet Study Facility that will validate a novel in situ RASL-Seq platform for diabetes research using FFPE tissue from normal and Type 1 diabetes (T1D) diseased pancreas, and then apply this platform to address the gap in available methodology to take full advantage of T1D organ
- [In Site TO-Seq Profiling of Gene Expression and Proteins in T2D](https://npod.org/current-npod-projects/in-site-to-seq-profiling-of-gene-expression-and-proteins-in-t2d/) - We will select T2D patients, among them subjects with DNA variants, and profile gene expression, expressed mutants, and proteins from sub-areas down to the level of single islets and single cells within archived pancreas FFPE tissues using a novel in situ TO-Seq assay coupled with immunohistochemistry staining of protein biomarkers, retaining the morphologic context of
- [IL-1beta expression in cystic fibrosis diabetes](https://npod.org/current-npod-projects/il-1beta-expression-in-cystic-fibrosis-diabetes/) - Cystic fibrosis related diabetes is a major morbidity for patients with cystic fibrosis and negatively impacts mortality. The mechanistic basis for this form of diabetes is not well understood. In this project we are analyzing the hypothesis that islet inflammation leads to islet amyloid deposition and beta cell failure in patients with CFRD. Samples from
- [Pancreatic expression of chemokines in human type 1 diabetes](https://npod.org/current-npod-projects/archived-projects/pancreatic-expression-of-chemokines-in-human-type-1-diabetes/) - Human chemokine superfamily of molecules mediate the recruitment of leukocytes to sites of inflammation. Many chemokines and chemokine receptors have emerged as key contributors and regulators of autoimmune disorders, including Type 1 Diabetes and may serve as potential surrogate biomarkers of inflammation or possible drug targets. More than one-half of all human and rodent chemokines
- [Hormonal regulation of adipose tissue macrophage functions in diabetes](https://npod.org/current-npod-projects/hormonal-regulation-of-adipose-tissue-macrophage-functions-in-diabetes/) - Our research is focused on the understanding of hormonal control of adipose tissue macrophage (ATM) function. ATMs play key roles in diabetes development: when they adopt an inflammatory activation state, they inhibit insulin signaling, cause systemic insulin resistance, exacerbate autoimmunity and pancreatic beta cell destruction. On the contrary, when ATMs undergo a so-called alternative activation,
- [“Empty Beta Cells” in type 1 diabetes](https://npod.org/current-npod-projects/empty-beta-cells-in-type-1-diabetes/) - During the pathogenesis of Diabetes Mellitus, whether it be type 1 or 2 diabetes, islet cell heterogeneity becomes apparent. This is most commonly seen as a marked variability between the insulin content of some ß-cells versus others. Indeed, some ß-cells have such low insulin content that they appear to be empty. As such, in using
- [Detection of islet autoreactive CD8 T-cells in insulitis versus periphery](https://npod.org/current-npod-projects/detection-of-islet-autoreactive-cd8-t-cells-in-insulitis-versus-periphery/) - To determine the specificities and frequencies of islet autoreactive CD8 T-cells in pancreas draining lymph nodes, control lymph nodes, spleen and/or peripheral blood from diabetic and non-diabetic organ donors, we used the Diab-Q-kit, a nanotechnology that allows the direct detection of circulating autoreactive CD8 T-cells against an array of islet-epitopes simultaneously, reducing the needs of
- [Immunochip Assays](https://npod.org/current-npod-projects/immunochip-assays/) - nPOD participated in the ImmunoChip consortium, that was established to design a cost effective genotyping array in order to fine map well established GWAS reported risk loci in immunologically related human diseases, including type 1 diabetes.
- [Assessment of memory T cells in the insulitis lesion](https://npod.org/current-npod-projects/assessment-of-memory-t-cells-in-the-insulitis-lesion/) - There is growing evidence that memory T cells are associated with a variety of autoimmune conditions, including type 1 diabetes (T1D). While autoreactive T cells are detected in both patients and healthy control subjects, CD45RO+ memory autoreactive T cells are usually found in patients but not in controls. Our own studies in several pancreas transplant
- [Epigenetic regulation of beta-cell function and identity](https://npod.org/current-npod-projects/epigenetic-regulation-of-beta-cell-function-and-identity/) - Diabetes is a disorder of complex interaction between genetic susceptibility and environmental perturbation. Various epigenetic models, such as intra-uterine growth retardation, have been shown to contribute to pancreatic beta-cell dysfunction. More recently, beta-cell dedifferentiation has been discovered as a mechanism for beta-cell dysfunction in diabetic mouse model. The project aim to understand the role of
- [Disruption of tolerance by ER-stress in type 1 diabetes](https://npod.org/current-npod-projects/disruption-of-tolerance-by-er-stress-in-type-1-diabetes/) - Type 1 diabetes (T1D) is an autoimmune disease in which insulin-producing islet cells are targeted and destroyed by the immune system, especially by T cells. The events that lead to immune recognition of islet proteins in people genetically predisposed to autoimmunity are poorly understood. Many of the environmental triggers associated with T1D including viral infection,
- [Linking IHC immune profiles of duodenum and pancreas in T1D](https://npod.org/current-npod-projects/linking-ihc-immune-profiles-of-duodenum-and-pancreas-in-t1d/) - Our project “Linking IHC profiles of duodenum and pancreas in T1D” aims to intensify our understanding of how the “gut immune system” contributes to the development of type 1 diabetes. Previous studies have shown that factors like the gut microbiome, gut permeability and the mucosal structure play a role in T1D pathogenesis. Studies are now
- [Role of influenza viruses in the etiopathogenesis of diabetes](https://npod.org/current-npod-projects/role-of-influenza-viruses-in-the-etiopathogenesis-of-diabetes/) - The rapid worldwide incidence increase suggests a major role for environmental factors in the aetiology of type 1 diabetes (T1D). According to cross-sectional and prospective studies on T1D patients and/or prediabetic individuals, virus infections may be one of these. Influenza viruses are known to cause severe pancreatic damage in birds and in some mammals, and
- [Identification of T1D-specific Fab fragments of IA-2 dominant conformational epitope](https://npod.org/current-npod-projects/identification-of-t1d-specific-fab-fragments-of-ia-2-dominant-conformational-epitope/) - One of the primary characteristics of Type 1 Diabetes (T1D) is the presence of autoantibodies directed to self-antigens expressed in the pancreatic islets, specifically those associated with the insulin secretory machinery. Although T1D is well characterized as a T-cell mediated disease, a mechanistic role of islet autoantibodies generated during the progression of T1D have not
- [12/15 Lipoxygenase expression in type 1 diabetes](https://npod.org/current-npod-projects/1215-lipoxygenase-expression-in-type-1-diabetes/) - 12/15-lipoxygenase (12/15-LO) is an enzyme responsible for the metabolism of arachidonic acid to pro-inflammatory responses by immune cells in a variety of tissues, including pancreatic islets and fat tissues. Our extensive research has shown that type 1-like diabetes development is significantly diminished in the absence of 12/15-lipoxygenase. By using 12/15-LO deficient C57BL/6 mice, we were
- [Protein based biomarkers for type 1 diabetes](https://npod.org/current-npod-projects/protein-based-biomarkers-for-type-1-diabetes/) - We have previously used Imaging Mass spectrometry (IMS) to identify Insulin within β-cells in the islets of Langerhans. The analysis utilized specially prepared pancreatic sections from the Network of Pancreatic Organ Donors with Diabetes (nPOD). In these studies, a comparative analysis of T1D MS spectra to those of Non-T1D revealed several uniquely expressed proteins between
- [Differentially expressed genes in inflamed human islets](https://npod.org/current-npod-projects/differentially-expressed-genes-in-inflamed-human-islets/) - The downregulation of cyclin D3 due to infiltration is causally related to beta cell dysfunction and beta cell-apoptosis in beta cells from NOD mice. We would like to confirm whether this is true in human beta cells. In order to validate cyclin D3 as a target for T1D therapy in humans, we need to evaluate the
- [Genetics of nPOD](https://npod.org/current-npod-projects/genetics-of-npod/) - We have found that people with type 1 diabetes fall into one of six subtypes that can be defined on the basis of the genetic variants they have. These subtypes differ with respect to various clinical features, including immunological traits and risk of diabetic complications. In this proposal, we plan to use genetic information from
- [In vivo imaging of islets](https://npod.org/current-npod-projects/in-vivo-imaging-of-islets/) - The aim of our study is to develop an imaging agent for in vivo determination of beta-cell mass in humans. Such an imaging agent will not only provide information on progression and management of diabetes but it will also help in monitoring an individual’s response to exercise, diet and anti-diabetic therapy. Unfortunately, there aren’t any
- [Tissue-selective chemokines and adhesion molecules in human T1D](https://npod.org/current-npod-projects/tissue-selective-chemokines-and-adhesion-molecules-in-human-t1d/) - Migration of lymphocytes from blood vessels into pancreatic lymph nodes (PanLNs) and pancreas is critical for the development of islet inflammation and beta cell destruction in type 1 diabetes (T1D). Adhesion of lymphocytes to the luminal surface of blood vessel endothelia in PanLNs and pancreas is an important step in this migration. However, the adhesion
- [Generation of tools to distinguish human pancreatic cell populations](https://npod.org/current-npod-projects/generation-of-tools-to-distinguish-human-pancreatic-cell-populations/) - Our work aims to understand the defects were deficiencies in the pancreatic beta cells of diabetics. We use a number of molecular methods, including but not limited to single cell RNA sequencing, to characterize the molecular phenotypes of islet cells.
- [Microangiopathy in diabetic bone marrow](https://npod.org/current-npod-projects/microangiopathy-in-diabetic-bone-marrow/) - We hypothesize that the alterations seen in diabetic BM are a major cause of impaired homeostasis in other vascular beds. Main objectives (1) to obtain a deeper insight into the cellular and vascular composition of diabetic BM using IHC, flow cytometry, and expressional studies (2) to determine whether BM microangiopathy is associated to poor metabolic control and increased susceptibility
- [Beta-cell stress signatures and infection in T1D islets](https://npod.org/current-npod-projects/beta-cell-stress-signatures-and-infection-in-t1d-islets/) - This project will be the first to examine stress granule responses and the expression of long non coding RNAs in donor islet cells, a model beta cell line and the role of enterovirus infection in modulating these stress responses. This information will be used as a benchmark to examine nPOD tissues for these same stress
- [Virome and microbiome in T1D onset](https://npod.org/current-npod-projects/virome-and-microbiome-in-t1d-onset/) - T1D is recognized to result from both genetic and environmental factors. Chief among environmental factors that are strongly linked to T1D are Type B enteroviruses (HEV-B), yet the association of these viral triggers is not proven and many questions exist. Which of the 60 HEV-B serotypes trigger T1D, what type of infections do they cause
- [ORIGINAL: Beta-cell regeneration by transdifferentiation ADDENDUM: Assessment of Islet Cell Transdifferentiation in Type 1 Diabetes](https://npod.org/current-npod-projects/beta-cell-regeneration-by-transdifferentiation/) - ORIGINAL: We have been interested in the mechanisms by which beta-cells regenerate in diabetes. Our laboratory is interested in how beta -cells regenerate under normal and pathophysiological conditions, with the goal of developing new therapies for diabetes that result in an increased number of those cells. There are two possible mechanisms for endogenous regeneration: beta-cell
- [Risk of autoimmune disease and human self-antigen expression](https://npod.org/current-npod-projects/risk-of-autoimmune-disease-and-human-self-antigen-expression/) - We have identified type 1 diabetes (T1D)-associated differential DNA methylated variable positions (T1D-MVPs) in CD14+ from peripheral blood mononuclear cells (PBMCs) from monozygotic (MZ) twins discordant for T1D (Rakyan V et al.; Plos Genetics 2011). We aim to determine the role of T1D-associated MVPs in the mechanisms that underpin immune dysfunction in T1D. We have therefore
- [Identification of a gene regulating pancreatic beta cell replication](https://npod.org/current-npod-projects/identification-of-a-gene-regulating-pancreatic-beta-cell-replication/) - We cloned a genetic modifier of T2D in obese mice. The gene, Ildr2, encodes a molecule that plays a role in both lipid metabolism and cellular responses to stress. Animals with lower levels of expression of this gene has reduced number of insulin producing cells in the pancreas, and, ultimately went on to develop mild diabetes. How
- [E-cadherin mediates developmental effects on the proliferation and the function of β-cells in the islet of Langerhans](https://npod.org/current-npod-projects/e-cadherin-mediates-developmental-effects-on-the-proliferation-and-the-function-of-beta-cells-in-the-islet-of-langerhans/) - In rodents and humans, the rate of beta cell proliferation declines rapidly after birth; formation of the islets of Langerhans begins perinatally and continues after birth. We tested whether increased levels of E-cadherin during islet formation mediate the decline in beta cell proliferation rate by contributing to a reduction of molecules that mediate cell replication. In vitro, a
- [Beta-cell mass in Antibody-positive non-diabetic subjects](https://npod.org/current-npod-projects/beta-cell-mass/) - The question of the B-cell mass of non-diabetic autoantibody-positive (Ab+) subjects is crucial to understand both common forms of type 1 diabetes and latent autoimmune diabetes (LADA). It is well established that the presence of GAD Abs is not sufficient to predict diabetes or insulin dependency. Knowing the B cell mass of Ab+ donors could
- [Do de-granulated ß-cells persist in type 1 diabetes?](https://npod.org/current-npod-projects/do-de-granulated-s-cells-persist-in-type-1-diabetes/) - The goal of this proposal is to define the lineage mechanism of ß-cell persistence in type 1 diabetes (T1DM). A few ß-cells often persist in T1DM pancreata. But, the lineage mechanism of ß-cell persistence in T1DM remains poorly understood. Our overarching hypothesis is that ß-cell persistence in T1DM is due to ongoing ß-cell regeneration. Alternatively,
- [ARC, a novel beta cell survival factor in type 2 diabetes](https://npod.org/current-npod-projects/arc-a-novel-beta-cell-survival-factor-in-type-2-diabetes/) - The progression of type 2 diabetes is mediated to a large extent by the deaths of β-cells, the cells in the pancreas that synthesize and secrete insulin. We discovered that ARC (Apoptosis Repressor with CARD), an endogenous inhibitor of cell death, is expressed at high levels in β-cells of the mouse pancreas. Using cell culture
- [Joslin Medalist Study](https://npod.org/current-npod-projects/joslin-medalist-study/) - History of the Joslin Medalist Program Joslin first began awarding medals to people with diabetes in 1948 with a 25-year Victory Medal. Believing that proper self-management was the key to minimizing long-term complications, the program was the vision of Elliott P. Joslin, M.D. and served as an incentive for those committed to good, though challenging,
- [Investigating islet alpha-to-beta cell conversion in Diabetes](https://npod.org/current-npod-projects/investigating-islet-alpha-to-beta-cell-conversion-in-diabetes/) - Diabetes occurs when insulin producing pancreatic β-cells are impacted: type 1 diabetes due to destruction of β-cells, and type 2 diabetes due to a decrease in their number and/or potency. Consequently, therapy that aims to replace β-cells is a promising long term alternative to the currently prevalent insulin injections. However, the difficulty in finding viable
- [Tracing effector and regulatory T cell populations in type 1 diabetes](https://npod.org/current-npod-projects/tracing-effector-and-regulatory-t-cell-populations-in-type-1-diabetes/) - Our group is studying T cell phenotype and antigen presentation and recognition by T cells in T1D, with three main objectives. (i) The characterization of role of T cells involved in immunoregulation, i.e. regulatory T cells (nTregs or iTregs) and NKT cells. We are studying the mechanisms of regulation by analyzing the role of soluble
- [Investigating the de-differentiated state of the β-cell in human diabetic patient tissues](https://npod.org/current-npod-projects/investigating-the-de-differentiated-state-of-the-beta-cell-in-human-diabetic-patient-tissues/) - It is well accepted that compromised ß-cell function is an integral part of diabetes development. Several recent studies, mostly using complex genetic mouse models, have supported the notion that a change in the ß-cell state or identity due to varying insults causes diabetes in the absence of ß-cell death. Our work has focused on the
- [Determination of specific and non-specific binding of 18F-FP-DTBZ in whole pancreas homogenates obtained from controls and patients with longstanding type 1 diabetes](https://npod.org/current-npod-projects/determination-of-specific-and-non-specific-binding-of-18f-fp-dtbz-in-whole-pancreas-homogenates-obtained-from-controls-and-patients-with-longstanding-type-1-diabetes/) - In the past we identified Vesicular Monoamine Transporter Type 2 (VMAT2), as a biomarker of beta cell mass that is quantifiable in vivo by Positron Emission Tomography (PET). PET is a tomographic imaging technique providing accurate non-invasive dynamic measurements of regional PET tracer uptake and clearance. These measurements are used to calculate VMAT2 density in
- [Beta-cell destruction and preservation in early and late onset type 1 diabetes](https://npod.org/current-npod-projects/beta-cell-destruction-and-preservation-in-early-and-late-onset-type-1-diabetes/) - Type 1 diabetes (T1D) is one of the most common chronic diseases of childhood with onset peaking between 5-7 years of age and around puberty. Early childhood-onset T1D often exhibits severe beta-cell depletion compared to late adult onset T1D. We propose to test the hypothesis that immature beta-cells in early childhood are more susceptible to
- [Fas Ligand: Unorthodox target for prevention of type 1 diabetes](https://npod.org/current-npod-projects/fas-ligand-unorthodox-target-for-prevention-of-type-1-diabetes/) - Despite improvement in insulin delivery, maintaining tight control of glucose homeostasis continues to be a challenge that results in bouts of severe hypoglycemia and hyperglycemia and serious long term complications in many patients. Therefore, developing an immunotherapy for type 1 diabetes (T1D) remains a major goal. Reaching this goal requires detailed knowledge of the diabetogenic
- [Diabetogenic function of autoimmune donor splenocytes in humanized mice](https://npod.org/current-npod-projects/diabetogenic-function-of-autoimmune-donor-splenocytes-in-humanized-mice/) - Our understanding of type 1 diabetes (T1D) has been advanced greatly by studies carried out using mice and rats. However, rodents are not humans and progress in the understanding of the pathogenesis of T1D in humans has been impeded by the a lack of assays that permit the direct in vivo analysis of diabetogenic human
- [Investigate the molecular link between increased risk of diabetes among patients with psychiatric disorders](https://npod.org/current-npod-projects/investigate-the-molecular-link-between-increased-risk-of-diabetes-among-patients-with-psychiatric-disorders/) - Compared to the general population, studies show 2-3 times increased risk of developing metabolic syndrome in drug naïve patients with severe mental illnesses like schizophrenia or bipolar disorder. Impaired glucose metabolism in non-obese never-treated patients and in first degree relatives of schizophrenic patients, suggests a possible genetic association between diabetes and schizophrenia. We are proposing
- [Bone marrow progenitor cell (BMPCs) dysfunction in diabetes is mediated by reduced bioavailability of NO](https://npod.org/current-npod-projects/bone-marrow-progenitor-cell-bmpcs-dysfunction-in-diabetes-is-mediated-by-reduced-bioavailability-of-no/) - Despite advances in understanding the pathogenesis of diabetic retinopathy (DR), the nature and time course of the molecular changes associated with DR remain incompletely understood. We propose to investigate the mechanism of bone marrow (BM) failure in diabetes and test diverse therapeutic approaches to correct BM and bone marrow progenitor cell (BMPC) dysfunction in order
- [Maternal microchimerism in T1D pancreas](https://npod.org/current-npod-projects/maternal-microchimerism-in-t1d-pancreas/) - Maternal microchimerism (MMc) is acquired by an infant during pregnancy and these cells are maintained in some individuals for decades. Encountering maternal antigens during pregnancy represents the first immunological challenge for the fetus although maternal cells are protected from detection by induction of suppressive fetal regulatory T cells. In type 1 diabetes increased levels of
- [Molecular insights into the type 1 diabetes pancreas](https://npod.org/current-npod-projects/molecular-insights-into-the-type-1-diabetes-pancreas-2/) - Pancreatic lymph nodes (PLN) are the central location for the autoimmune response that leads to type 1 diabetes (T1D), yet no histological studies of this crucial step in pathogenesis of human diabetes exist. In this study, we request sections from T1D and matched control PLN to allow us to follow up our preliminary observations showing
- [Characterization of type 1 diabetes subsets](https://npod.org/current-npod-projects/characterization-of-type-1-diabetes-subsets-3/) - Type 1 diabetes is a clinically and pathologically heterogeneous disease. A large subset of individuals with Type 1 diabetes has an autoimmune form of the disease characterized by subtotal loss of beta cells and positivity for islet autoantibodies as well an increase prevalence of DR3 and DR4. We have recently characterized a pathological pattern of beta cell loss
- [Analysis of normal pancreas from PNET patients with and without diabetes](https://npod.org/current-npod-projects/analysis-of-normal-pancreas-from-pnet-patients-with-and-without-diabetes/) - Spontaneous recovery from established type 1 diabetes (T1D) is so rare that only one well-documented case has ever been described in an adult, and in that case recovery resulted from an insulin-secreting tumor, not regeneration of the patient's normal beta-cells. Therefore, when we saw a 50 year old man at UCSF with T1D who got
- [Different expression of extracellular CAR in islets in pancreatic sections and isolated islets](https://npod.org/current-npod-projects/different-expression-of-extracellular-car-in-islets-in-pancreatic-sections-and-isolated-islets/) - The etiology of the type 1 diabetes (T1D) is unclear but it has been shown that genetic factors influence the pathogenesis. In addition, several studies have shown that environmental factors likely contribute to the disease development. One such factor is virus infections, particularly the Coxsackie B viruses (CBVs), are among the main candidates and there
- [Role of TMEM219 Expression in Type 1 Diabetes](https://npod.org/current-npod-projects/role-of-tmem219-expression-in-type-1-diabetes/) - The recent failure of immunotherapeutic strategies in the cure of T1D raised questions in considering autoimmunity the sole mechanism responsible for the pathogenesis of T1D. It has been recognized that activation of immune response against self-peptides is essential in the onset of T1D, but its role in the destruction of beta cells, which leads to
- [Microarray analysis of PLN from autoantibody positive donors](https://npod.org/current-npod-projects/microarray-analysis-of-pln-from-autoantibody-positive-donors/) - As with many autoimmune diseases, T1D is initiated by some unknown inciting event (or events) that results in the appearance of disease-relevant biomarkers. Serum autoantibodies to islet antigens or changes in whole blood gene expression that appear before the onset of glucose intolerance can serve as biomarkers to identify pre-diabetic individuals. As we enter the
- [Generation of pluripotent stem cells from diabetics ADDENDUM: Generation of iPS cell lines from a majority of T1D cases](https://npod.org/current-npod-projects/generation-of-pluripotent-stem-cells-from-diabetics/) - ORIGINAL PROJECT: Established different skin fibroblast cell lines, both from control and T1D nPOD donors. Graciously offered this as a resource available to current nPOD investigators. ADDENDUM: The pancreata of nPOD cases are some of the best characterized type 1 diabetes cases. The availability of beta cells from stem cells would greatly enhance this resource
- [Beta cell defects in cystic fibrosis related diabetes](https://npod.org/current-npod-projects/beta-cell-defects-in-cystic-fibrosis-related-diabetes/) - The aim of this study is to determine how CFTR mutations affect beta cell performance, and whether their effect is direct (cell-autonomous), or indirect, by affecting other cell types/physiological processes with secondary consequences for beta cell function and viability. It is likely that any mechanistic insights obtained from the study of this specific etiology of
- [Identification of islet associated immune cells in type 2 diabetic patients and further exploration of their role in disease progression and severity](https://npod.org/current-npod-projects/identification-of-islet-associated-immune-cells-in-type-2-diabetic-patients-and-further-exploration-of-their-role-in-disease-progression-and-severity/) - Growing evidence suggests inflammation as a major underlying mechanism in the pathogenesis of type 2 diabetes mellitus (T2DM). Supporting the involvement of the immune system in T2DM, cross-sectional and prospective studies have associated elevated circulating levels of acute-phase proteins. Furthermore, clinical studies have demonstrated that anti-inflammatory drugs may improve glycaemia. Morphological and therapeutic intervention studies
- [Analysis of the antigen-specific T cell repertoire in T1D patients](https://npod.org/current-npod-projects/analysis-of-the-antigen-specific-t-cell-repertoire-in-t1d-patients/) - The majority of human T cell antigens relevant to T1D have been defined using algorithms which predict high affinity peptide binding to MHC. However, direct evidence showing that these antigens are presented to T cells by human islets and APCs in T1D patients has not been established for the vast majority of these peptides. A
- [Characterization of autoantigen-specific T cell receptors from PLNs](https://npod.org/current-npod-projects/characterization-of-autoantigen-specific-t-cell-receptors-from-plns/) - Type 1A Diabetes mellitus (T1D) is a predominantly T cell mediated autoimmune endocrine disease. Both CD4+ and CD8+ T cells reactive to diabetic autoantigens can be identified at low frequency in the peripheral blood of diabetic subjects and their at-risk relatives, but the extent to which these peripheral responses reflect those in the pancreatic draining
- [Molecular signatures of islet inflammation in type 1 diabetes](https://npod.org/current-npod-projects/molecular-signatures-of-islet-inflammation-in-type-1-diabetes/) - The process of β-cell decline in the lead-up to the overt diabetes could provide critical clues for timely therapeutic intervention. However the complexity, cell-type specificity, rapidly changing dynamics and the cumulative effects of inflammation all inform the outcome of the autoimmune attack on beta cell. This proposal is designed to use multiple in vitro models
- [IL-21/IL-21R and GLP-1R staining on pancreas tissues from (pre-)T1D, T2D and control donors](https://npod.org/current-npod-projects/il-21il-21r-and-glp-1r-staining-on-pancreas-tissues-from-pre-t1d-t2d-and-control-donors/) - Our aim is to assess the protein expression level in conjunction with histological and (sub)cellular localization of the cytokine IL-21, its receptor IL-21R and GLP-1R in normal controls, (pre-)type 1 and type 2 diabetics (with history of incretin treatment for GLP-1R). (adopted by nPOD staff)
- [TCR signal transduction in diabetogenic T cells](https://npod.org/current-npod-projects/tcr-signal-transduction-in-diabetogenic-t-cells/) - Tyrosine kinase inhibitors (TKis) effectively limit autoimmunity in animal models and are under clinical study in RA, MS and psoriasis. Recent studies in NOD mice have demonstrated proof-of-principal for promiscuous PTK blockade, using inhibitors initially developed to inhibit oncogenic pathways in cancer. Our recent work has shown that the selective Syk inhibitor R788 limits diabetes
- [Persistent organic pollutants in the pancreatic tissue of people with and without diabetes](https://npod.org/current-npod-projects/persistent-organic-pollutants-in-the-pancreatic-tissue-of-people-with-and-without-diabetes/) - There is now strong evidence that persistent organic pollutants (POPs) such as polychlorinated biphenyls (PCBs) are major risk factors for type 2 diabetes. While the mechanisms involved are not known with any certainty, the associations between development of type 2 diabetes and blood concentrations of POPs are stronger than those for almost any other factor,
- [Imaging pancreatic beta-cells with PET neuroimaging agents](https://npod.org/current-npod-projects/imaging-pancreatic-beta-cells-with-pet-neuroimaging-agents/) - A clinically viable means to measure pancreatic beta-cell mass (BCM) is essential for evaluating the physiological basis of therapeutic approaches to restore deficient insulin secretory capacity. Major advances in imaging BCM have been made by taking advantage of receptor-specific imaging probes that have been successfully used for neuroimaging. Hence, Positron Emission Tomography (PET) imaging ligands
- [Diabetes and Pancreatic Cancers: Pilot study with molecular profiling](https://npod.org/current-npod-projects/archived-projects/diabetes-and-pancreatic-cancers-pilot-study-with-molecular-profiling/) - Our study aims are the following: 1. Histopathology review of pancreata from patients without diabetes (no diabetes and autoantibody+) and those within the diabetic and other groups (type 1, type 2, gestational, other (those with unclassified diabetes, excludes cystic fibrosis)). 2. Molecular profiling of PanNET for neuroendocrine hormones and other genes (Isl1, p53, Ki67), FISH
- [Mapping the histopathological landscape of human T1D: a pilot study (CLARITY)](https://npod.org/current-npod-projects/mapping-the-histopathological-landscape-of-human-t1d-a-pilot-study/) - The overall goal of this project is to extend studies examining human beta cells and immune cells in type 1 diabetes (T1D) to a new 3D fluorescent microscopy format for 500um or greater sized samples. These studies will adapt new tissue imaging methods (CLARITY) developed by Dr. K. Deisseroth (Nat Methods 2013). The specific aims
- [Tracking the loss of anergic B cells in pre-diabetic and new onset T1DHLA Testing](https://npod.org/current-npod-projects/tracking-the-loss-of-anergic-b-cells-in-pre-diabetic-and-new-onset-t1dhla-testing/) - Insulin-specific B lymphocytes play an important role in development of autoimmunity in Type 1 Diabetes (T1D). Since potentially offensive autoreactive cells are silenced by mechanisms of immune tolerance, participation of insulin-binding B cells (IBCs) in T1D must reflect escape from this silencing. Examining this question, we found that those IBCs bearing antigen receptors with high
- [Pancreatic duct glands in type 1 diabetes](https://npod.org/current-npod-projects/pancreatic-duct-glands-in-type-1-diabetes/) - The initial overall question to be tested by the collaborative of the Peter Butler laboratory at UCLA and the Mark Atkinson laboratory in Florida was to explore the possibility that the recently described pancreatic duct gland (PDG) compartment of the pancreas might serve as the elusive pancreatic stem cell niche, potentially offering an avenue to
- [Extracellular matrix involvement in type 1 diabetes pancreatic islet destruction](https://npod.org/current-npod-projects/extracellular-matrix-involvement-in-type-1-diabetes-pancreatic-islet-destruction/) - The overall purpose of our research is to understand how changes in extracellular matrix affect insulitis initiation and progression and beta cell destruction in diabetes. Our research focuses on a specific extracellular matrix molecule, hyaluronan, a long chain glycosaminoglycan distributed widely throughout different tissues. Hyaluronan is highly abundant in inflamed tissues and its synthesis is
- [Vascular endothelial growth factor receptor signaling regulates the pathogenesis of type 1 diabetes](https://npod.org/current-npod-projects/vascular-endothelial-growth-factor-receptor-signaling-regulates-the-pathogenesis-of-type-1-diabetes/) - 1. To test the hypothesis that vascular endothelial cells in the pancreas of recent-onset diabetic patients have increased expression of VEGF receptors (VEGF-R). 2. To examine the expression of VEGF or VEGF-R on islet-invading CD4+ T lymphocytes. 3. To examine the utility of TCR repertoire between different T cell subsets as a marker of T1D
- [Beta-cell Death and Survival](https://npod.org/current-npod-projects/beta-cell-death-and-survival/) - Type 1 diabetes (T1D) is a progressive immune-mediated disease that is preceded by an asymptomatic preclinical period of highly variable duration. A better understanding of beta cell and islet function or dysfunction during this asymptomatic period and the nature of the “dialog” between islet endocrine cells and the immune system could lead to new therapeutic
- [Innate immunity and diabetes](https://npod.org/current-npod-projects/innate-immunity-and-diabetes/) - Although T1D is considered to be mainly a T-cell mediated and a beta-cell specific disease we have generated over the years the hypothesis (and some supporting data) that innate immunity is also key in disease initiation and that T1D might be a disease of the whole pancreas that manifests in autoimmunity to beta cells. Our
- [Dissecting the effector/regulatory compartments in the target tissues of T1D](https://npod.org/current-npod-projects/dissecting-the-effectorregulatory-compartments-in-the-target-tissues-of-t1d/) - An altered balance between pathogenic and regulatory pathways in autoimmunity has been hypothesized and at times demonstrated in peripheral blood of patients with type 1 diabetes. However, our knowledge in humans at the sites of the autoimmune attack (i.e., the pancreas) is scanty. Studies generated in our lab. demonstrate that FOXP3+ T regulatory (Treg) cells,
- [Fibroblast Activation Protein in Type 1 Diabetes](https://npod.org/current-npod-projects/fibroblast-activation-protein-in-type-1-diabetes/) - Fibroblast activation protein is an extracellular membrane bound and soluble protease that is commonly used as a marker of activated fibroblasts. Expression of FAP in healthy adult tissues is limited, but up-regulation is observed in a number of inflammatory and tissue remodeling processes, including wound healing, liver fibrosis and epithelial cancer, where it is thought
- [Beta cell replication](https://npod.org/current-npod-projects/beta-cell-replication/) - Recent decades have seen an incredible growth in the prevalence of diabetes. While it is important to pursue multiple therapeutic strategies, harnessing the regenerative capacity of islet β-cells to increase an individual's insulin secretion capacity is among the promising approaches. Recently, discovery-oriented unbiased chemical screening led to the identification of the metabolic enzyme adenosine kinase
- [Identification of novel Tissue-Specific Antigens expressed by human Extrathymic Aire-Expressing Cells, and determination of their potential contribution to the prevention of type 1 diabetes](https://npod.org/current-npod-projects/identification-of-novel-tissue-specific-antigens-expressed-by-human-extrathymic-aire-expressing-cells-and-determination-of-their-potential-contribution-to-the-prevention-of-type-1-diabetes/) - Aire+ cells were readily identified by immuno-staining in both PN and nPLN sections. Like in mouse, human eTACs were relatively rare but localized outside the B-cell follicles and stained with the hallmark “nuclear speckling” pattern. Furthermore, the eTACs were ubiquitously positive for MHC class II but lacked high expression of CD11c, calling into question their
- [Beta cell dedifferentiation in type 2 diabetes](https://npod.org/current-npod-projects/beta-cell-dedifferentiation-in-type-2-diabetes/) - Diabetes is associated with β-cell dysfunction. But it remains unclear whether the latter results from reduced β-cell number or function. Purpose of this study is to investigate the hypothesis that β-cell failure is the result of β-cell dedifferentiation, rather than apoptosis or degranutaion. Transcription factor FoxO1 integrates β-cell proliferation with adaptive β-cell function. The rationale
- [Comparison of total pancreatic insulin content and peripheral markers of beta cell function and desctruction](https://npod.org/current-npod-projects/comparison-of-total-pancreatic-insulin-content-and-peripheral-markers-of-beta-cell-function-and-desctruction/) - In both Type 1 and Type 2 diabetes, there is a gradual loss of pacreatic beta cells with time. The degree of beta cell destruction may be indirectly measured by the appearance of islet-specific components in circulation. For instance, insulin mRNA is increased in circulation in T1D patients before clinical loss of function of transplanted
- [Intrinsic immunoregulatory defect of bone marrow mesenchymal stem cells (BM-MSC) from T1D patients](https://npod.org/current-npod-projects/intrinsic-immunoregulatory-defect-of-bone-marrow-mesenchymal-stem-cells-bm-msc-from-t1d-patients/) - Type 1 diabetes (T1D) represents a major public health burden demanding innovative treatment strategies. Mesenchymal stem cells (MSC) have profound immunomodulatory effects. Numerous clinical trials have focused on the immunomodulatory capacity of MSC to treat various immune-mediated diseases. We were amongst the first to show the protective effects of allogeneic MSC in autoimmune diabetes in
- [Identification of protective niches in T1D pancreata](https://npod.org/current-npod-projects/identification-of-protective-niches-in-t1d-pancreata/) - Type 1 diabetes (T1D) is caused by an autoimmune attack mediated by cytotoxic T cells. However as the attack unfolds, many other cell types participate–and some play an anti-inflammatory, regulatory, role. Interestingly, in the early stages of the disease, attacked islets often reside next to non-attacked islets. We hypothesized that the “tranquil” islets harbor elements
- [The nPOD-Virus Group](https://npod.org/current-npod-projects/the-npod-virus-group/) - nPOD has now assembled a “cloud” of investigators with diverse expertise, who are interested in collaborative studies, and is ready to take the nPOD research model to a higher level: the nPOD working groups. These groups are intended to collectively tackle key questions in diabetes research. The nPOD virus working group (nPOD-V) represents a self-assembled
- [Identification of HERV-W copies and insertion sites associated with T1D in human genomes](https://npod.org/current-npod-projects/identification-of-herv-w-copies-and-insertion-sites-associated-with-t1d-in-human-genomes/) - T1D etiology is not clearly understood, but it lies at crossroads between genetic predisposition and environmental factors. Research on the human genome has so far identified numerous candidate sequences as genetic risk factors, most notably within the HLA (Human Leucocyte Antigen) region. However, an important part of the human genome has been neglected in the
- [Enteroviral infection as a causative factor in type 1 diabetes](https://npod.org/current-npod-projects/enteroviral-infection-as-a-causative-factor-in-type-1-diabetes/) - Considerable evidence has accumulated implying that enteroviral infection is associated with the development of type 1 diabetes but the precise nature of this relationship remains unclear. Many studies have revealed that enteroviral RNA is detectable in patient serum at, or before, the onset of disease but it is unclear whether viral infection also occurs commonly
- [Virus detection in pancreas and other tissues](https://npod.org/current-npod-projects/virus-detection-in-pancreas-and-other-tissues/) - Enterovirus infections cause pancreatitis and damage endocrine cells in the pancreatic islets. Epidemiological studies have shown an association between enterovirus infections and type 1 diabetes, and viral structures have been detected in the pancreas of diabetic patients. Our recent epidemiological studies have suggested that among all different enterovirus types, the group of coxsackie B viruses
- [Mononuclear phagocyte populations in T1D islets](https://npod.org/current-npod-projects/mononuclear-phagocyte-populations-in-t1d-islets/) - Little is known about how T cell pathogenesis is modulated in the islets during T1D, yet this is a critical obstacle for preventing destruction of remaining islets or beta cell grafts. Therefore, we must elucidate mechanisms that promote and prevent the pathogenic autoimmune response in the islets. Our data in the NOD mouse model show
- [Diabetes Virus Detection Study (DiViD)](https://npod.org/current-npod-projects/diabetes-virus-detection-study-divid/) - The Diabetes Virus Detection Study (DiViD) is the first to examine fresh pancreatic tissue at the diagnosis of type 1 diabetes (T1D) for the presence of viruses. Surgical minimal pancreatic tail resection was performed in general anesthesia 3-9 weeks after onset of type 1 diabetes in six adult patients (age 24-35 years). Pancreatic tissues were
- [IAPP and innate immunity in T1D](https://npod.org/current-npod-projects/iapp-and-innate-immunity-in-t1d-2/) - Islet amyloid polypeptide (IAPP) is a beta-cell peptide hormone that forms toxic and pro-inflammatory aggregates in pathological states, including T2D and islet transplants. Recent evidence suggests the presence of fibrillar aggregates of IAPP (islet amyloid) in some T1D pancreatic islets. In T2D and mouse models of islet amyloid formation, aggregates of human IAPP recruit and
- [Understanding diabetes progression via tissue chemical imaging](https://npod.org/current-npod-projects/understanding-diabetes-progression-via-tissue-chemical-imaging/) - Type 1 diabetes (T1D) is a devastating multifactorial disorder manifested by the autoimmune destruction of insulin-producing beta cells leading to insulin deficiency. Our overarching aim is to investigate the spaciochemical changes in the human exocrine and endocrine pancreata during various stages of T1D with unmatched chemical specificity and multiplexity. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry
- [Insulin Receptor on T cells in T1D](https://npod.org/current-npod-projects/insulin-receptor-on-t-cells-in-t1d/) - T cell movement into the islets in the pancreas, is a hallmark feature of Type 1 diabetes (T1D) that has been demonstrated on autopsy in human pancreas specimens and in mouse models of T1D such as the non-obese diabetic (NOD) mouse3-8. By determiningthe role that insulin receptor (IR)plays in this movement of the T cells
- [The sensory innervation of the pancreatic islet in health and diabetes](https://npod.org/current-npod-projects/the-sensory-innervation-of-the-pancreatic-islet-in-health-and-diabetes/) - The role the nervous system plays in modulating islet inflammation and diabetes pathogenesis has barely been examined. Recent papers suggest that changes in sensory innervation initiate autoimmune diabetes in mice, which is in line with the notion that sensory neurons contribute to inflammation in several chronic disorders. Thus, the local interactions between the immune system
- [Single cell analysis of the human pancreas in type 1 diabetes](https://npod.org/current-npod-projects/single-cell-analysis-of-the-human-pancreas-in-type-1-diabetes/) - Type 1 diabetes (T1D) is an autoimmune disorder that affects 1.25 million individuals in the US and for which there is no known cause or cure. T1D is characterized by autoimmune destruction of insulin- producing beta cells in the pancreatic islets. The pancreas consists of multiple different cell-types relevant to diabetes pathogenesis including endocrine (beta,
- [Transcriptional heterogeneity in the intact human pancreas](https://npod.org/current-npod-projects/transcriptional-heterogeneity-in-the-intact-human-pancreas-2/) - Pancreatic beta cells have been shown to be heterogeneous at multiple levels. However, spatially interrogating transcriptional heterogeneity in the intact tissue has been challenging. We developed an optimized protocol for single-molecule transcript imaging in the intact pancreas and used it to identify a sub-population of “extreme” beta cells with elevated mRNA levels of insulin and
- [High-resolution mapping of the human pancreatic magnetic resonance landscape](https://npod.org/current-npod-projects/high-resolution-mapping-of-the-human-pancreatic-magnetic-resonance-landscape/) - Magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) both detect signals from atomic nuclei and can be performed together in conventional clinical MRI scanners. MRI provides the anatomical information, after which MRS scanning can provide biochemical information on the constituents within a region of interest. MRS has been used in clinical settings to study
- [Urocortin 3 as a sensitive marker for beta cell function](https://npod.org/current-npod-projects/urocortin-3-as-a-sensitive-marker-for-beta-cell-function/) - Urocortin 3 (Ucn3) is a neuropeptide that was discovered by our group (Lewis et al., 2001). Ucn3 is specifically expressed in mouse beta cells, but is in human islets also expressed by alpha cells (Li et al., 2003; van der Meulen et al., 2012). Ucn3 is a member of the same neuropeptide family as corticotropin-releasing
- [Meta - proteomic biomarkers in stool for prediction of islet autoimmunity progression](https://npod.org/current-npod-projects/meta-proteomic-biomarkers-in-stool-for-prediction-of-islet-autoimmunity-progression/) - Type 1 diabetes (T1D) is increasing around the world due to unknown changes in our lifestyle. One factor that may drive this change is the bacteria in our intestines. These bacteria interact potently with our immune system and metabolism. We have shown that changes in intestinal and pancreatic proteins found in stool are associated with
- [Targeting Islet-Antigen Specific B Cells for Deletion as a Means to Prevent Type 1 Diabetes](https://npod.org/current-npod-projects/targeting-islet-antigen-specific-b-cells-for-deletion-as-a-means-to-prevent-type-1-diabetes/) - Type 1 Diabetes (T1D) is an autoimmune disease in which the body attacks and destroys its own insulin-producing β-cells. It affects over 3 million people in the United States. For most T1D patients, it is extremely difficult to achieve normal glycemic control even with frequent blood glucose measurements, diet control, careful determination of insulin dosage,
- [Characterizing T-Cell Populations in Diabetes](https://npod.org/current-npod-projects/characterizing-t-cell-populations-in-diabetes/) - This study seeks to understand the immune cells important for beta cell loss by studying the function and type of cells present in lymphoid tissue.
- [Redox regulation of anti-viral responses in type 1 diabetes](https://npod.org/current-npod-projects/redox-regulation-of-anti-viral-responses-in-type-1-diabetes/) - Type 1 diabetes (T1D) is a T cell-mediated autoimmune disease resulting in the destruction of pancreatic beta-cells. Infiltrating leukocytes generate an inflammatory environment consisting of reactive oxygen species (ROS) and proinflammatory cytokines that collectively participate in beta-cell destruction and enhance the adaptive immune effector response of islet-specific T cells. Environmental factors that include viral infections
- [Analysis of innate immune response of myeloid cells in human Type 1 diabetes](https://npod.org/current-npod-projects/analysis-of-innate-immune-response-of-myeloid-cells-in-human-type-1-diabetes-2/) - Type 1 diabetes (T1D) is a multifactorial autoimmune disease that requires genetic susceptibility as well as environmental triggers to induce disease onset. Genetic association studies have implicated the IFIH1 gene, encoding for melanoma differentiation-associated protein 5 (MDA5), a cytosolic sensor of dsRNA, to be involved in T1D susceptibility and resistance. Stimulation of the MDA5 signaling
- [Analysis of Immunocytes in Human Islets of Type 1 Diabetes Patients](https://npod.org/current-npod-projects/analysis-of-immunocytes-in-human-islets-of-type-1-diabetes-patients/) - CD4+, CD8+ T cells and B cells will be isolated from islets of patients suffering from type 1 diabetes. Laser dissection will be used to localize and isolate single cells after tissue staining and confocal imaging at high resolution. T cell receptors and B cell receptors will be isolated from single cells and expressed to
- [Decoding and Reprogramming Stem-Like Progenitor CD8 T cells Driving Type 1 Diabetes](https://npod.org/current-npod-projects/decoding-and-reprogramming-stem-like-progenitor-cd8-t-cells-driving-type-1-diabetes/) - The pathogenesis of T1D is complex and involves many distinct immune populations. Autoreactive CD8 T cells play a major role as the ultimate mediators of beta cell destruction in the pancreas, leading to the loss of glucose homeostasis. However, many aspects of the programming and regulation of CD8 T cells mediating autoimmunity remain enigmatic. Utilizing
- [Correlation of Islet Autoantigen-Specific T-Cell Repertoire in the Pancreatic Lymph Nodes and Peripheral Blood in T1D Autoimmunity](https://npod.org/current-npod-projects/correlation-of-islet-autoantigen-specific-t-cell-repertoire-in-the-pancreatic-lymph-nodes-and-peripheral-blood-in-t1d-autoimmunity/) - Islet autoantigen specific T cells can be found in both T1D patients and healthy individuals. However, these T cells with the same antigen specificity are expected to differ in their phenotypic characteristics. We will investigate CD4 and CD8 T cells in nPOD samples from T1D patients, prediabetic and healthy subjects allowing us to compare T
- [The role of shared germline-like TCR alpha chains of self-reactive TCRs in type 1 diabetes](https://npod.org/current-npod-projects/the-role-of-shared-germline-like-tcr-alpha-chains-of-self-reactive-tcrs-in-type-1-diabetes/) - Human islet antigen reactive CD4+ memory T cells (IAR T cells) play a key role in the pathogenesis of autoimmune type 1 diabetes (T1D). Using single cell RNA-sequencing (scRNA-seq) to identify T cell receptors (TCRs) in IAR T cells, we identified a multi-specific class of T1D-associated T cell receptors (TCRs) that share TCR alpha chains
- [Credentialing immunotherapy targets in islet tissues with insulitis](https://npod.org/current-npod-projects/credentialing-immunotherapy-targets-in-islet-tissues-with-insulitis/) - Sanofi is developing strategies to control beta cell destruction in type 1 diabetes (T1D) through immunotherapy. Strategies could include targeting specific molecules on cells that may be of importance in disease pathogenesis. Such approaches are in progress and candidate molecular targets of potential interest have been identified. As part of the next steps, our goal
- [Investigation of B cells in human islets and PLN in T1](https://npod.org/current-npod-projects/investigation-of-beta-cells-in-human-islets-and-pln-in-t1/) - Our goal is to understand the role of autoreactive B cells in tissues draining the pancreas and their role in the type 1 diabetes (T1D) autoimmune response. While B cells are responsible for secreting autoantibodies, B cells have other functions including secretion of effector cytokines, antigen presentation and costimulation of T cell responses. Using a
- [Characterization of T Cells that Recognize Post-Translationally Modified Beta Cell Epitopes from within the PLN](https://npod.org/current-npod-projects/characterization-of-t-cells-that-recognize-post-translationally-modified-beta-cell-epitopes-from-within-the-pln/) - We have recently demonstrated that enzymatically modified peptides derived from beta cell antigens are preferentially recognized by autoreactive CD4+ T cell clones isolated from the peripheral blood of patients with T1D. Furthermore, T cells that recognize these modified peptides are present in the peripheral blood of patients with T1D and have a high affinity, enabling
- [Hybrid peptides as target antigens for pathogenic T cells in human T1D patients](https://npod.org/current-npod-projects/hybrid-peptides-as-target-antigens-for-pathogenic-t-cells-in-human-t1d-patients/) - We have recently discovered a new form of beta-cell autoantigen, which is recognized by CD4 T cells, and is formed through a novel post-translational modification of beta-cell peptides that involves the fusion between insulin peptide fragments and peptides of other secretory granule proteins. The hybrid insulin peptides (HIPs) are highly antigenic for autoreactive and pathogenic
- [T follicular helper (TFH) and regulatory (TFR) cells in T1D](https://npod.org/current-npod-projects/t-follicular-helper-tfh-and-regulatory-tfr-cells-in-t1d/) - Type 1 Diabetes (T1D) is characterized by the gradual loss of insulin-producing beta cells, which are eliminated by autoreactive cells infiltrating the pancreas. In humans and mouse models of the disease, T1D susceptibility is determined by genetic factors and is greatly influenced by environmental triggers (1). Progression to T1D is usually preceded by a period
- [Molecular Signature of Autoimmune T cells in Type 1 Diabetes and Treg TCR deep sequencing. Detect Cd226+ Treg and Teff ininsulitis patient samples](https://npod.org/current-npod-projects/molecular-signature-of-autoimmune-t-cells-in-type-1-diabetes-and-treg-tcr-deep-sequencing-detect-cd226-treg-and-teff-ininsulitis-patient-samples/) - The Brusko Lab is generally interested in the cellular immune response involved in the disease process leading to type 1 diabetes (T1D). A strong genetic association between T1D and the MHC class II gene region of the adaptive immune system has implicated coordinated T and B cell responses as a central mediator of the disease
- [Use of T1D pancreastic and lymphoid tissue to assess the expression of adhesion molecules](https://npod.org/current-npod-projects/use-of-t1d-pancreastic-and-lymphoid-tissue-to-assess-the-expression-of-adhesion-molecules/) - While immunoregulatory therapies hold great promise for the treatment of T1D, their inadequacy and serious toxicity have limited research efforts to find a lifelong immunosuppression approach. This has motivated investigators to search for alternative approaches such as the use of nanotechnology to deliver a wide variety of drugs and biomolecules. In recent years, there have been
- [Northwest Lipid Research](https://npod.org/current-npod-projects/northwest-lipid-research/) - The Marcovina lab performs C-peptide testing for all nPOD samples.
- [UF AAb Core](https://npod.org/current-npod-projects/uf-aab-core/) - The Autoantibody Core at the University of Florida serves as the coordinating center for nPOD screening lab partners (LABS,Inc and Viracor-IBT Laboratories). Combi-kit manufacturing and quality control/assurance measures are managed here. Additionally, the UF Autoantibody Core tests all nPOD samples for autoantibodies to GAD, IA-2, and ZnT8 by ELISA.
- [Nanotomy of human islets of Langerhans in type 1 diabetes](https://npod.org/current-npod-projects/nanotomy-of-human-islets-of-langerhans-in-type-1-diabetes/) - Microscopy is of paramount importance in diabetes research: Paul Langerhans was the first to describe (1869) Islets based on a light microscopic (LM) evaluation of the pancreas. (Immuno-)EM-analysis of the Islets of Langerhans is an outstanding way to discriminate beta-cells from other endocrine cells: The crystalline appearance of insulin granules makes beta-cells readily distinguishable from
- [MODY mutation testing](https://npod.org/current-npod-projects/mody-mutation-testing/) - Perform Maturity Onset Diabetes of the Young (MODY) testing for select nPOD samples.
- [Pancreatic immunologic and metabolic parameters](https://npod.org/current-npod-projects/pancreatic-immunologic-and-metabolic-parameters/) - The Organ Processing and Pathology Core (OPPC), located at the University of Florida, receives nPOD donor tissue directly from OPOs, processes the tissue, ships samples directly to investigators and stores case samples and data.
- [Distribution of neuropeptide FF in the human infant pancreas](https://npod.org/current-npod-projects/distribution-of-neuropeptide-ff-in-the-human-infant-pancreas/) - Improving adipose tissue (AT) insulin resistance and induce adipocyte browning are key therapeutic aims in the management of obesity associated insulin resistance (IR) and in type 2 diabetes (T2DM). Our laboratory has recently identified neuropeptide FF (NPFF) as a pancreatic hormone, which selectively improves IR in the AT and controls AT browning, especially in infancy
- [Multidisciplinary approaches to driving human beta cell replication](https://npod.org/current-npod-projects/multidisciplinary-approaches-to-driving-human-beta-cell-replication/) - Understanding and controlling human beta cell replication and regeneration are major goals of the Breakthrough T1D specifically, and of the diabetes community in general. Whereas the field of beta cell replication is a relatively new one, and much new data has recently accumulated regarding the molecular control of rodent beta cells, very little is known
- [Expression of Beta-Cell Dedifferentiation-Associated  microRNAs in Type 2 Diabetes](https://npod.org/current-npod-projects/expression-of-beta-cell-dedifferentiation-associated-micrornas-in-type-2-diabetes/) - MicroRNAs are small endogenous RNAs which regulate gene expression through mRNA decay or translational inhibition. It has been previously demonstrated that microRNAs are pivotal regulators of cell phenotype and fate and are involved in many biological processes such as proliferation, differentiation or apoptosis in a wide type of cells and organisms. Given their prominent role
- [Probing the normal human pancreatic and islet matrisome throughout development](https://npod.org/current-npod-projects/probing-the-normal-human-pancreatic-and-islet-matrisome-throughout-development/) - Diabetes is a major world health problem, affecting millions of Americans, costing billions of dollars in treatment, and causing major late-stage health complications and death. Despite the promise of stem cell therapies for diabetes, the field of beta cell differentiation has not yet reached clinical application, mainly due to the lack of mature function and
- [Cell conversion in the human pancreas](https://npod.org/current-npod-projects/cell-conversion-in-the-human-pancreas/) - The overall goal of my proposal is to identify means for improving β-cell regeneration in the adult pancreas. We previously developed a transgenic model of inducible total or partial β-cell ablation (termed RIP-DTR). We have reported that in these mice there is spontaneous reconstitution of new β-cells from heterologous (i.e. non-β) cells after near-total β-cell
- [Progenitors of the beta cell lineage in the pancreas and biliary tree of diabetic patients](https://npod.org/current-npod-projects/progenitors-of-the-beta-cell-lineage-in-the-pancreas-and-biliary-tree-of-diabetic-patients/) - Stemming from a collaboration with Lola Reid (University of North Carolina), we have described stem and progenitor cells in niches of the human biliary tree and pancreatic ducts. These cells can mature into pancreatic endocrine cells, including functional insulin-producing cells. Our data suggest that bile ducts, peribiliary glands, pancreatic ducts and pancreatic duct glands may
- [ORIGINAL: Beta cell regeneration for Type 1 Diabetes ADDENDUM: Neuro-insular pathways regulating beta cell regeneration](https://npod.org/current-npod-projects/beta-cell-regeneration-for-type-1-diabetes/) - ORIGINAL: Understanding basic human beta cell physiology is crucial to targeted therapies and prevention strategies for type 1 diabetes. Clinical signs in type 1 diabetes start when beta cell function and/or numbers are unable to maintain adequate blood glucose levels. After onset of type 1 diabetes, life-long insulin replacement therapy is needed. Factors regulating human
- [Epigenetic analysis of nPOD islet cells](https://npod.org/current-npod-projects/epigenetic-analysis-of-npod-islet-cells/) - Type 1 Diabetes (T1D) is a complex autoimmune disease affecting more than 30 million people worldwide. T1D is caused by a combination of genetic and epigenetic factors leading to immune destruction of insulin-secreting islet cells. Although significant progress has recently been made in elucidating the genetics of T1D, the non-genetics component has remained poorly defined.
- [Quantitative and qualitative evaluation of the endocrine & exocrine pancreas in type 1 diabetes](https://npod.org/current-npod-projects/the-ductal-pancreatic-progenitor-microenvironment-in-type-1-diabetes/) - In the most widely recognized natural history model for type 1 diabetes, there is a presumption that all individuals begin life with the same number of beta cells. In combination with this, a consensus exists that the so-called “normal” adult pancreas possesses approximately one million islet cells. These concepts have drawn little attention or question
- [Preventive Insulin Therapy for Type 2 Diabetes (Addendum: Pancreatic beta cell heterogeneity; Addendum: Heterogeneity of human beta cells)](https://npod.org/current-npod-projects/preventive-insulin-therapy-for-type-2-diabetes/) - Two major pathophysiologic abnormalities underlie most cases of type 2 diabetes (T2D): insulin resistance and defects in pancreatic beta-cell function. These defects are initially compensated by an increase in insulin secretion and in the number of insulin secreting cells. However, with time, beta cell dysfunction and T2D develops, requiring intervention therapies. Eventually, there is significant
- [Pathogenic Action of Autoantibodies on Islet Function and Viability in Type 1 Diabetes](https://npod.org/current-npod-projects/pathogenic-action-of-autoantibodies-on-islet-function-and-viability-in-type-1-diabetes/) - Type 1 Diabetes (T1D) is known to develop over time when autoimmune processes attack and destroy beta cells thereby reducing the capacity to secrete adequate amounts of insulin. The relatively gradual progression of the disease offers an opportunity for therapeutic intervention to slow or prevent destruction of beta cells before the disease has reached symptomatic
- [Lipid droplet distribution study in human pancreas](https://npod.org/current-npod-projects/lipid-droplet-distribution-study-in-human-pancreas/) - In collaboration with Dr. Alvin Powers’ at Vanderbilt University, we showed by electron microscopy (EM) that transplanted human islets have a much higher level of lipid droplets (LDs) than transplanted mouse islets (1). Moreover, we found that the number of LDs in transplanted human islets was increased in high fat diet (HFD) fed mouse recipients.
- [Heparan sulfate levels mark the health status of human islet β-cells](https://npod.org/current-npod-projects/heparan-sulfate-levels-mark-the-health-status-of-human-islet-beta-cells/) - Heparan sulfate (HS) is a sulfated glycosaminoglycan that consists of repeating disaccharides (composed of uronic acid and glucosamine), forming a linear polysaccharide. The HS chains are assembled onto the core proteins of heparan sulfate proteoglycans (HSPGs). We recently reported that HS is localized in the peri-islet basement membrane (BM) in mice and is intensely expressed
- [VDAC1 a Common Denominator of Pancreatic β-cell and Vascular Endothelial Cell Apoptosis in T1D and its Organ Complications](https://npod.org/current-npod-projects/vdac1-a-common-denominator-of-pancreatic-β-cell-and-vascular-endothelial-cell-apoptosis-in-t1d-and-its-organ-complications/) - Impaired insulin secretion from the β-cells characterizes both type 2 and the initial phase of type 1 diabetes (T1D). Although the two sub-forms have different causes, we postulate that the rapidly developing β-cell defect and death in T1D as well as the slowly progressing β-cell dysfunction associated with T2D share a common cellular mechanism. Long-term
- [Pancreatic islet biology and vascularization](https://npod.org/current-npod-projects/pancreatic-islet-biology-and-vascularization/) - Our group is interested in pancreatic islet development and function and how beta cells are affected in diabetes. Critical discoveries from nPOD samples and resources are changing our understanding of type 1 diabetes. We are using or hope to use nPOD samples for the following studies: 1) Define and describe the normal human pancreas and
- [Validating KIRAs as β-cell-sparing drugs in islets from human patients with T1D](https://npod.org/current-npod-projects/validating-kiras-as-β-cell-sparing-drugs-in-islets-from-human-patients-with-t1d/) - Our lab has been focusing for many years on the intrinsic stress experienced by pancreatic islet β-cells, which (even in healthy states) live under a strict metabolic mandate to continuously produce and secrete insulin at intensely high rates that predispose these professional secretory cells to secretory exhaustion. β-cells evolved a high-capacity endoplasmic reticulum (ER) organelle
- [Deoxyhypusine synthase: a novel target for beta cell protection](https://npod.org/current-npod-projects/deoxyhypusine-synthase-a-novel-target-for-beta-cell-protection/) - The cellular processes giving rise to type 1 diabetes (T1D) and type 2 diabetes (T2D) involve the activation of inflammation, which leads to the eventual death of islet β cells. An urgent priority in diabetes research is the discovery of biomarkers (simple blood tests) that can assist in the identification of persons at-risk for disease
- [Islet resistance to T1D](https://npod.org/current-npod-projects/islet-resistance-to-t1d/) - Our proposed studies will address a major gap in the understanding of T1D, namely, to identify the mechanisms of beta cell failure from a metabolic as well as an immunological perspective. This specific project has high potential to reveal significant, novel, and translatable information regarding beta cell dysfunction that will be of therapeutic benefit for
- [Identification of novel monoclonal antibodies directed against pancreatic endocrine cell types for in vitroand in vivo analysis and targeting](https://npod.org/current-npod-projects/identification-of-novel-monoclonal-antibodies-directed-against-pancreatic-endocrine-cell-types-for-in-vitroand-in-vivo-analysis-and-targeting/) - Diabetes mellitus is a multifactorial disease characterized by progressive loss or dysfunction of the insulin-producing beta cells in the pancreas that leads to chronic hyperglycemia, systemic metabolic complications and multi-organ damages. Nowadays, no current treatments can stop or reverse the disease progression, apart bariatric surgery and human islet transplantation. Therefore, intensive efforts in the field
- [IGF receptor-like 1 and 2, new therapeutic targets for increasing insulin sensitivity and beta cell health](https://npod.org/current-npod-projects/igf-receptor-like-1-and-2-new-therapeutic-targets-for-increasing-insulin-sensitivity-and-beta-cell-health/) - Diabetes mellitus is a complex and multifactorial disease characterized by progressive loss or dysfunction of the insulin-producing beta cells in the pancreas. This results in chronic hyperglycemia and systemic metabolic complications and, in the long-term, in multi-organ damages; together, these complications create enormous medical and social burdens as well as causing premature deaths. Today, over
- [The role inflammatory signaling crosstalk on human exocrine and beta cells](https://npod.org/current-npod-projects/the-role-inflammatory-signaling-crosstalk-on-human-exocrine-and-beta-cells/) - There is increasing evidence for cross-talk between the islet and exocrine compartments of the pancreas. Diabetes is a risk factor for exocrine atrophy, pancreatitis, and pancreatic cancer. Conversely, pancreatitis is a risk factor for diabetes. Until now our ability to study human endocrine and exocrine cells has been limited to isolated islets and isolated exocrine
- [Insulin interacting proteins and stress markers in T1D](https://npod.org/current-npod-projects/insulin-interacting-proteins-and-stress-markers-in-t1d-2/) - We have developed novel methods for proteomic study of beta-cells within native islets. Using antibodies to proinsulin or insulin we reported the first Biosynthetic Interaction Network for insulin (Pottekat, Cell Reports, 2013). Based upon our finding that many proteins which interact directly with insulin are T1D GWAS candidates or proteins required for ER homeostasis, we
- [Defining islet heterogeneity using single islet transcriptomics](https://npod.org/current-npod-projects/sequencing-the-islet-transcriptome/) - The goal of this project is to obtain transcriptome data from human islets using either hybridization arrays or RNA sequencing technology. We plan to obtain complete information about relative expression of all genes in islets from a number of different organ donor groups with an emphasis on: 1) donors with no autoantibodies or diabetes, 2)
- [Regulation of Beta Cell Secretory Pathway Calcium Homeostasis in Type 1 Diabetes](https://npod.org/current-npod-projects/regulation-of-beta-cell-secretory-pathway-calcium-homeostasis-in-type-1-diabetes/) - Calcium plays a vital role in many processes that govern beta cell function, including the production, maturation, and regulated secretion of insulin. The fidelity of these processes depends on the maintenance of calcium subcompartments and their respective transmembrane gradients, which are organized at both the cellular and organelle level. Then endoplasmic reticulum, Golgi apparatus, and
- [Characterization of beta cell ER stress associated to enteroviral infection in type 1 diabetes](https://npod.org/current-npod-projects/characterization-of-beta-cell-er-stress-markers-in-type-1-diabetes/) - Enteroviral infections are associated with an increased risk to develop type 1 diabetes. Several stains of enteroviruses are able to infect beta cells leading to both inflammation and cell death, two major factors for the onset of type 1 diabetes (T1D). The cellular factors that modulate cellular permissiveness to enteroviral infection and beta cell death
- [Markers of stress in diabetic islets](https://npod.org/current-npod-projects/markers-of-stress-in-diabetic-islets/) - Our project seeks to understand the molecular basis for beta cell failure in human diabetes. We have discovered that excessive glucose metabolism in beta cells may lead to double strand breaks in the DNA and activation of the tumor suppressor gene p53. With the help of nPOD, we are studying the significance if this response
- [Beta cell DNA double strand breaks in Type 1 diabetes](https://npod.org/current-npod-projects/beta-cell-dna-double-strand-breaks-in-type-1-diabetes/) - Emerging evidence suggests beta cell dysfunction and failure precede autoimmune destruction during the development of type 1 diabetes, but the underlying mechanisms are not known. Defining and targeting the changes leading to impaired beta cell function may lead to clinical improvement and prolonged honeymoon period. We have recently discovered that in type 2 diabetes, beta
- [Role of myosin-I in insulin secretion](https://npod.org/current-npod-projects/role-of-myosin-i-in-insulin-secretion/) - In pancreatic beta cells, insulin is produced as proinsulin and packaged into secretory granules at a region of the cell called the trans-Golgi network (TGN). After maturation which includes the conversion of proinsulin to insulin, insulin granules are trafficked to the cell membrane where their contents are released through a process known as exocytosis. Actin
- [Origin of composite islet structure in humans](https://npod.org/current-npod-projects/composite-islet-structure-in-humans/) - In the adult human pancreas the majority of islets have a clear non-random organization of mantle of non beta cells around beta cell cores, with larger islets usually having multiple such subunits (composite) whereas smaller islets resembled the classic rodent pattern. There is far more variability in islet composition both within each human pancreas and
- [Mechanisms Involved in Expansion of Beta Cells in Pregnancy](https://npod.org/current-npod-projects/mechanisms-involved-in-expansion-of-beta-cells-in-pregnancy/) - Pregnancy is associated with a marked increase in the number of pancreatic beta cells in response to the increased insulin demand. In rats and mice the growth of the beta cell mass is primarily due to proliferation of the existing beta cells resulting in enlargement of the islets of Langerhans. However, in a study of
- [Aging Beta Cell](https://npod.org/current-npod-projects/aging-beta-cell/) - Type 2 diabetes (T2D) increases with age with the majority of patients being above the fifth decade of life. These data underline the importance of studying how aging contributes to the reduced beta cell mass and dysfunctional insulin secretion found in T2D. However, the specific contribution of beta cell aging and senescence to diabetes has
- [Uncovering pathological factors and mechanisms leading to β-cell death in type 1 diabetes](https://npod.org/current-npod-projects/uncovering-pathological-factors-and-mechanisms-leading-to-β-cell-death-in-type-1-diabetes/) - We hypothesize that β-cell toxicity in T1D can be induced via different pathways by distinct pathological ligands (AGEs, S100-proteins, amyloidogenic amylin) of the receptor for advanced glycation endproducts (RAGE) that become upregulated in the diabetic pancreas. We also hypothesize that C-peptide (+) T1D subjects may be more susceptible to amylin-induced β-cell toxicity. Amylin is co-produced /co-secreted with
- [Role of Alpha Cells in Pathogenesis of Type 1 Diabetes](https://npod.org/current-npod-projects/role-of-alpha-cells-in-pathogenesis-of-type-1-diabetes-2/) - Multiple islet autoantibodies (AAb+) predict type 1 diabetes (T1D) and hyperglycemia within 10 years. By contrast, T1D develops in just ~15% of single AAb+ (generally against glutamic acid decarboxylase, GADA+) individuals; hence the single AAb+ state may represent an early stage of T1D. We previously discovered the functional defects in suppression of glucagon secretion in
- [Islet Pathology in Cystic Fibrosis](https://npod.org/current-npod-projects/islet-pathology-in-cystic-fibrosis/) - Insulin deficiency is a common feature of cystic fibrosis (CF), resulting in CF-related diabetes (CFRD) in ~20% of adolescents and ~50% of adults with the disease. The clinical consequences of insulin deficiency in CF are significant – CFRD is associated with worsened CF lung disease and increased morbidity and mortality. The loss of exocrine pancreatic
- [Preventing beta cell dedifferentiation in type 1 diabetes](https://npod.org/current-npod-projects/preventing-beta-cell-dedifferentiation-in-type-1-diabetes/) - Recent evidence suggests that beta cell dedifferentiation is a key early mechanism that underlies the pathogenesis of type 2 diabetes (T2D) [1]. This includes the loss of transcriptional regulators of beta cell function as well as other critical components of beta cell function. The study further suggests that beta cell dedifferentiation and acquisition of progenitor markers precedes
- [Role of bacterial biofilms in the onset of pancreas inflammation in T1D](https://npod.org/current-npod-projects/role-of-bacterial-biofilms-in-the-onset-of-pancreas-inflammation-in-t1d/) - Even though the immunopathogenic triggering event(s) in type 1 diabetes (T1D) remain enigmatic, there is accumulating evidence, mainly in rodent models of the disorder, suggesting that pancreas-resident antigen-presenting cells (APC) are activated very early post-natally. This results in the recruitment of innate immune cells like neutrophils into the pancreas even before adaptive immune cells that
- [De-differentiation during progression of beta cell loss in type 1 diabetes](https://npod.org/current-npod-projects/de-differentiation-during-progression-of-beta-cell-loss-in-type-1-diabetes/) - The overall goal of this proposal is to test the hypothesis that β-cell de-differentiation is an early stress-related response that may account for the early loss of β-cells in T1D. Furthermore, we believe that a small but significant population of long-term T1D patients might still have de-differentiated β-cells in their pancreatic islets, which creates hope
- [Target confirmation in human pancreas](https://npod.org/current-npod-projects/title-pending/) - Type 1 Diabetes (T1D) is caused by autoimmune-induced beta-cell destruction and successful beta-cell regeneration offers a potential cure to the disease. We have identified a beta-cell regenerative target that leads to the restoration of beta-cell mass in mouse models of beta-cell deficiency. We seek to confirm the observed cellular phenotype in human pancreatic samples.
- [Role of p21-activated secretory phenotype (PASP) in initiating type 1 diabetes pathogenesis](https://npod.org/current-npod-projects/role-of-p21-activated-secretory-phenotype-pasp-in-initiating-type-1-diabetes-pathogenesis/) - Type 1 diabetes (T1D) results from autoimmune insulitis destruction of insulin-secreting β cells. β cell senescence with upregulated expression of P21 has been observed in the adult NOD mice and new-onset patients. However, it remains unclear whether the beta cell senescence is an outcome of persistent insulitis, and the factors trigger insulitis remains unclear. The
- [Spatio-temporal visualization of immune and non-immune islet injury in Type 1 Diabetes](https://npod.org/current-npod-projects/spatio-temporal-visualization-of-immune-and-non-immune-islet-injury-in-type-1-diabetes/) - Type 1 Diabetes (T1D) generally results from a poorly understood autoimmune process that leads to selective destruction of pancreatic beta cells. Interestingly, beta cell function is often not correlated with mass and declines very early in the disease before symptom onset, but the mechanisms driving this are not known. Islet beta cells existing in a
- [Immediate-early gene transcription factor (IEG-TF) expression within human pancreatic islets during pregnancy and gestational diabetes](https://npod.org/current-npod-projects/immediate-early-gene-transcription-factor-ieg-tf-expression-within-human-pancreatic-islets-during-pregnancy-and-gestational-diabetes/) - Pregnancy is a unique physiologic state in adult females that occurs and resolves over a defined time period. The acute metabolic stress it causes on the mother triggers adaptations with maternal pancreatic islets. Failure of normal islet adaptation is a major contributor to gestational diabetes (GDM).In rodents, insulin-producing beta-cells proliferate causing expansion of beta-cell mass.
- [The insulin secretory granule components and its immune recognition in type 1 diabetes](https://npod.org/current-npod-projects/the-insulin-secretory-granule-components-and-its-immune-recognition-in-type-1-diabetes/) - Our parent project studies the intracellular accumulation and molecular interaction of proinsulin, insulin and the proinsulin processing enzymes PC1/3, PC2 and CPE, which may be dysregulated during the development of the disease and lead to processing errors. Our interest was focused on proinsulin and insulin, as these are the most abundant molecules of the insulin
- [Exploring an islet-protective role for native pancreatic mesenchymal stromal cells in health and in type 1 diabetes](https://npod.org/current-npod-projects/exploring-an-islet-protective-role-for-native-pancreatic-mesenchymal-stromal-cells-in-health-and-in-type-1-diabetes/) - Our research aims to investigate how a group of adult stem cells, known as mesenchymal stem /stromal cells(MSCs), located in the pancreas may be involved in helping to protect people from developing type 1 diabetes(T1D). MSCs are found in most parts (tissues) of the body, including the pancreas, bone marrow, fat and kidney. When samples
- [Mapping islet B cells in the pancreas on a single-cell level](https://npod.org/current-npod-projects/mapping-islet-b-cells-in-the-pancreas-on-a-single-cell-level/) - Recent evidence suggests a role for B cells in the pathogenesis of type 1 diabetes (T1D), particularly in individuals who develop T1D at a younger age and demonstrate rapid progression. However, little is known regarding the specificity, phenotype, and function of B cells in young-onset T1D. Recently we performed a cross-sectional analysis comparing insulin-reactive to
- [Unraveling the Role of CD45RA+CCR7+CD4 T Cells in the Pathogenesis of Type 1 Diabetes](https://npod.org/current-npod-projects/unraveling-the-role-of-cd45raccr7cd4-t-cells-in-the-pathogenesis-of-type-1-diabetes/) - This study aims to investigate the role of CD45RA+CCR7+CD4 T cells in the pathogenesis of type 1 diabetes (T1D). Historically, naïve T cells were deemed irrelevant in T1D development due to their presumed lack of antigen experience. However, distinct subsets of naïve T cells with varying phenotypes and functions have been identified, with recent literature
- [Unbiased single cell resolution spatial proteomics: defining the antigenic landscape in the context of HLA-I expression during type 1 diabetes progression](https://npod.org/current-npod-projects/unbiased-single-cell-resolution-spatial-proteomics-defining-the-antigenic-landscape-in-the-context-of-hla-i-expression-during-type-1-diabetes-progression-2/) - Diabetes is a complex metabolic disease in which patients experience high blood glucose levels leading to a plethora of symptoms and long-term complications. In type 1 diabetes (T1D), this happens likely due to an autoimmune reaction targeting the insulin producing cell population, so-called beta cells, within the pancreatic islets. CD8+ T-cells are considered the main
- [Targeting the innate immune system to discover new therapeutic pathways for the prevention of type 1 diabetes](https://npod.org/current-npod-projects/targeting-the-innate-immune-system-to-discover-new-therapeutic-pathways-for-the-prevention-of-type-1-diabetes-2/) - Type 1 diabetes (T1D) is an autoimmune disease, where immune cells (T cells) of the body attack and destroy the insulin-producing beta cells. T1D is, if left untreated, a devastating and life-threatening disease and despite intensive research in the field, there are still missing pieces in the pathogenetic puzzle of the disease. With this project,
- [CD6 and its ligands in Type 1 Diabetes](https://npod.org/current-npod-projects/cd6-and-its-ligands-in-type-1-diabetes-2/) - Type 1 diabetes mellitus (T1D) results from the autoimmune attack of pancreatic β-cells, resulting in a loss of functional β-cell mass. The CD6 molecule is a membrane glycoprotein predominantly expressed on T cells and is implicated in lymphocyte adhesion, activation, and proinflammatory commitment. The CD6 ligand CD166/ALCAM is upregulated in mature dendritic cells and the
- [Quantitative proteomic profiling of human islets in healthy and diabetes associated pregnancy](https://npod.org/current-npod-projects/quantitative-proteomic-profiling-of-human-islets-in-healthy-and-diabetes-associated-pregnancy/) - Normal pregnancy is associated with a rise in insulin secretion, which begins at placental implantation and increases progressively in parallel with placental growth. Following placenta delivery there is a sharp postpartum decline in insulin secretion. Gestational diabetes (GDM),the development of diabetes for the first time during pregnancy, is associated with pancreatic beta-cell dysfunction. Rodent models
- [Availability of nPOD Cases for Assay Validation and Generation of Preliminary Data](https://npod.org/news-events/availability-of-npod-cases-for-assay-validation-and-generation-of-preliminary-data/) - nPOD has now several available cases that have been retired for distribution to research projects, but can be used for assay validation or generation of preliminary data. Reasonable amounts of materials can be requested. Please contact Helmut Hiller if you would like to order samples from these cases. These specimens are available to approved nPOD investigators and to
- [Data Portal](https://npod.org/npod-webinars/past-webinars/data-portal/) - DATE: Thursday, September 22nd, 2022 TIME: 11:00am – 012:00pm EST Download Meeting Recording: Dropbox link to view meeting recording Discussion led by: Maria Beery, MS & Irina Kusmarteva, PhD
- [Autoimmunity Group Webinar](https://npod.org/npod-webinars/autoimmunity-group-webinar-2/) - DATE: Thursday, April 25th, 2019 Download Meeting Recording: Dropbox link to view meeting recording Discussion led by: Alberto Pugliese, MD and Sally Kent, PhD
- [Save the Date for the 15th Annual nPOD Scientific Meeting](https://npod.org/news-events/save-the-date-for-the-15th-annual-npod-scientific-meeting/) - Lorem ipsum dolor sit amet, consectetur adipiscing elit. Cras vitae rutrum nibh. Pellentesque interdum ultrices lorem nec tristique. Phasellus sit amet scelerisque magna. Cras pulvinar commodo sapien et luctus. Nulla facilisi. Phasellus tempus lorem neque, pretium congue tortor lacinia in. Cras feugiat vel est vel interdum. Integer semper gravida turpis vel volutpat. Donec volutpat vitae arcu quis venenatis. Aenean nec ex id neque faucibus eleifend.
- [NKG2D ligand expression in type 1 diabetes development](https://npod.org/current-npod-projects/nkg2d-ligand-expression-in-type-1-diabetes-development/) - Recent evidence derived from animal models of type 1 diabetes indicates that interaction of immune cells expressing the protein known as NKG2D with cells expressing other proteins, termed NKG2D ligands, plays an essential role in initiating diabetes development. In an effort to define more precisely the role these proteins play in type 1 diabetes progression,
- [Validation of blood - derived single cell gene expression signature in peri - pancreatic lymph nodes of Type 1 Diabetes subjects](https://npod.org/current-npod-projects/validation-of-blood-derived-single-cell-gene-expression-signature-in-peri-pancreatic-lymph-nodes-of-type-1-diabetes-subjects/) - Type 1 diabetes (T1D) is an organ specific autoimmune disease resulting in an immune-mediated destruction of insulin-secreting beta cells. The etiology of T1Dis puzzled by a multifaceted interaction of environmental, genetic and epigenetic factors leading to variousendotypesof the disease. Fine mapping of T1D-associated genetic variants with cell-specific epigenetic markers or expression quantitative traits (eQTLs) indicates
- [Viral sequence discovery by high throughput DNA sequencing of the nPOD diabetic pancreatic samples](https://npod.org/current-npod-projects/viral-sequence-discovery-by-high-throughput-dna-sequencing-of-the-npod-diabetic-pancreatic-samples/) - Several lines of evidence, in particular genetic association data of antiviral response genes with T1D risk, point to a role of viral infections in T1D etiology. However, multiple questions remain about the causal mechanisms and the type of viruses implicated. To contribute to these research questions, we are using high throughput sequencing technologies to find
- [Evaluation of HERV-W Envelope antigen expression in Pancreas and serum from patients with Type 1 Diabetes.](https://npod.org/current-npod-projects/archived-projects/evaluation-of-serum-from-patients-with-type-1-diabetes/) - Endogenous retroviruses are known to represent 8% of the Human genome. HERV-W family retains elements expressing an envelope protein (Env), which activates a pro-inflammatory and autoimmune cascade through interaction with Toll-Like receptor 4 (TLR4). This Env protein was evidenced in brain lesions, sera and circulating mononuclear cells of patients with Multiple Sclerosis (MS)1. Thus, all
- [To identify mRNA and miRNA biomarkers associated with exosomes that predict the onset of T1D](https://npod.org/current-npod-projects/to-identify-mrna-and-mirna-biomarkers-associated-with-exosomes-that-predict-the-onset-of-t1d/) - There are currently no clinically precise biomarkers that predict the development of type 1 diabetes (T1D). This limits the ability to identify patients who will become diabetic at a time when they have a greater beta cell mass and thus may respond more favorably to interventional therapeutics aimed at stopping T1D progression. To this end,
- [Assessing heterogeneity of DR3 haplotype risk in type 1 diabetes](https://npod.org/current-npod-projects/assessing-heterogeneity-of-dr3-haplotype-risk-in-type-1-diabetes/) - We have discovered a new DNA variation that we think may affect T1D risk in individuals carrying a particular type of HLA-DRB1 gene. We aim to look at particular nPOD samples with that HLA genotype to see if they contain our newly discovered variant. If so, we will be able to design a future project
- [Identifying and Validating Noncoding RNAs as Human Beta Cell-Specific Biomarkers](https://npod.org/current-npod-projects/identifying-and-validating-noncoding-rnas-as-human-beta-cell-specific-biomarkers/) - Direct identification and validation of human β cell-specific biomakers is critical for monitoring the change of human β cell mass at prediabetes and the new-onset diabetes stage and during the therapeutic intervention. Due to technical and ethical constraints, our ability to prospective studies of human β cell biomarkers in response to β cell stress/apoptosis and
- [Role of CCL21 in Directing Immune Invasion of Pancreatic Islets](https://npod.org/current-npod-projects/role-of-ccl21-in-directing-immune-invasion-of-pancreatic-islets/) - Type 1 diabetes results, at least in part, from invasion of pancreatic islets by T lymphocytes and antigen presenting cells (APCs) including macrophages and dendritic cells (DCs). What drives the migration of these immune cells into the islets remains unknown. A key step in the arrival of immune cells to a specific location is adhesion
- [Manipulating DNA damage-response signaling for the treatment of type 1 diabetes](https://npod.org/current-npod-projects/manipulating-dna-damage-response-signaling-for-the-treatment-of-type-1-diabetes/) - As T cell toggle through their various life cycle, certain distinct biological changes occur that can be specifically targeted with small molecule drugs to target these cells for cell death. This is particularly true of activated autoreactive T cells. We have developed a combination of small molecular drugs that can target activated T cells and
- [Tracking islet autoantigen specific CD4+ T Cells](https://npod.org/current-npod-projects/tracking-islet-autoantigen-specific-cd4-t-cells/) - The goal of this research project is to identify self-reactive T cell clones and monitor the frequency and activation state during the progression of autoimmune diabetes. Identification of T-cell reactivity to beta-cell antigen epitopes is important to study pathogenesis and monitor antigen specific interventions in T1D. We have successfully generated and validated human tetramer reagents
- [Analysis of type 1 interferon responses to rotavirus in patients with type 1 diabetes](https://npod.org/current-npod-projects/analysis-of-type-1-interferon-responses-to-rotavirus-in-patients-with-type-1-diabetes/) - Rotavirus infection of children at-risk of type 1 diabetes is associated with disease progression. Similarly, infection of adult non-obese diabetic (NOD) mice with Rhesus monkey rotavirus RRV accelerates diabetes onset. This acceleration correlates with RRV spread to the mesenteric and pancreatic lymph nodes, where virus associates with and activates dendritic cells and induces B and
- [Microtubule-associated protein TAU and the Islets of Langerhans](https://npod.org/current-npod-projects/microtubule-associated-protein-tau-and-the-islets-of-langerhans/) - Tau is one of the most important known molecules for microtubular dynamic and trafficking within the cells. In neurogenerative disorders such as Alzheimer disease, the tau becomes hyperphosporylated and form insoluble intracellular protein aggregates leading to upregulation of unfolded protein response. At the same time, patients with Alzheimer’s disease show impaired glucose metabolism. Moreover, both
- [Small RNA molecules (miRNAs, circRNAs, tRNAs, and more): relevance to Type 1 diabetes](https://npod.org/npod-webinars/past-webinars/small-rna-molecules-mirnas-circrnas-trnas-and-more-relevance-to-type-1-diabetes/) - DATE: Thursday, August 7, 2022 TIME: 11:00am - 1:00pm, EST Meeting Recording: Dropbox link to view meeting recording Discussion led by: Romano Regazzi, PhD and Alberto Pugliese, MD
- [nPOD - Transplantation](https://npod.org/news-events/npod-transplantation/) - Thanks to generous Breakthrough T1D support, nPOD has recently established the nPOD-Transplantation (nPOD-T) program. Building on the nPOD infrastructure, nPOD-T will collect tissues (transplanted and native pancreas, peripancreatic lymph nodes, spleen and peripheral blood) from patients with T1D who received a pancreas or pancreas/kidney transplants. Specifically, nPOD-T plans to: obtain donated organs/tissues from pancreas transplant
- [Changes in pericyte phenotype and function during type 1 diabetes](https://npod.org/current-npod-projects/changes-in-pericyte-phenotype-and-function-during-type-1-diabetes/) - Endocrine cells in pancreatic islets are embedded in a network of blood vessels that support adequate nutrient sensing, efficient release of hormones, and timely responses to changes in glycemia. This network of islet blood vessels consists of capillary tubes made of endothelial cells that are covered by pericytes. Compelling evidence indicates that significant structural alterations
- [Changes in human islet microvasculature during the progression of type 1 diabetes](https://npod.org/current-npod-projects/changes-in-human-islet-microvasculature-during-the-progression-of-type-1-diabetes/) - Endocrine cells in pancreatic islets are embedded in a network of microvessels that regulate islet blood flow and hormone secretion. It is still unknown what happens to the islet microvasculature in type 1 diabetes (T1D), despite being the site of immune cell recruitment and infiltration. The microvasculature consists of capillary tubes made of a thin
- [Role of environmental chemicals in the development of type 1 diabetes](https://npod.org/current-npod-projects/role-of-environmental-chemicals-in-the-development-of-type-1-diabetes/) - There is a well-documented rise in the incidence of type 1 diabetes (T1D) in industrialized countries suggesting a role for environmental chemical exposures. Alarmingly, the increased incidence is seen mostly in children under age 5. However, research on environmental chemical exposures and T1D is very limited and there is an urgent need for research to
- [Longitudinal Determination of Islet Specific protein expression in T1D](https://npod.org/current-npod-projects/longitudinal-determination-of-islet-specific-protein-expression-in-t1d/) - Pandion Therapeutics is a biotechnology company developing bifunctional protein therapeutics to achieve localized immunomodulation at the site of disease for durable, tissue-specific treatment of patients with autoimmune and inflammatory disease and organ transplant. Our localized approach represents a significant change from the systemic immunosuppression of conventional medicines. Through tissue-specific therapeutic targeting, Pandion’s approach has the
- [Maximizing Your nPOD Sample Request: Using Online Pathology and DataShare to Select the Right Cases for Your Study](https://npod.org/npod-webinars/maximizing-your-npod-sample-request-using-online-pathology-and-datashare-to-select-the-right-cases-for-your-study/) - DATE: Wednesday, November 30th, 2016 SPEAKERS: Maria Beery, MS nPOD Senior Biological Scientist Irina Kusmartseva nPOD Organ Processing and Pathology Core (OPPC) Director Download PowerPoint presentation. As any veteran nPOD investigator knows, every donor case is different. While it is tempting to order based on donor classification alone, we encourage our investigators to be highly
- [FOXO1 inhibition-mediated reprogramming of b-like cells in gastrointestinal tissues from type 1 diabetic donors](https://npod.org/current-npod-projects/foxo1-inhibition-mediated-reprogramming-of-b-like-cells-in-gastrointestinal-tissues-from-type-1-diabetic-donors/) - Genetic inactivation of the nuclear transcription factor Forkhead box protein O1 (FOXO1) in mice in specific intestinal endocrine progenitor cells reprograms them into glucose-responsive insulin- producing (beta-like) cells, raising the promise that the gut can serve as an endogenous source for beta cell replacement. This phenomenon has also been demonstrated in vitro in cells derived
- [Reconstitution of HHLS mice with bone marrow from patients with T1DM](https://npod.org/current-npod-projects/reconstitution-of-hhls-mice-with-bone-marrow-from-patients-with-t1dm/) - The focus of our Breakthrough T1D Autoimmunity Center is to create and study a humanized mouse model of Type 1 diabetes. The approach that we are developing involves use of immune deficient (γc-/-) mice with “knock-in” of human cytokine and other genes that are needed for optimal reconstitution with human cells. In addition, we have created transgenic mice that
- [ORIGINAL: Growth and Development of the Endocrine Pancreas in Humans: Extrapolating Normal Physiology to the Regenerative Capacity of the Endocrine Compartment in Type 1 and Type 2 Diabetes; ADDENDUM: The Role of Neuropeptide Y (NPY) in Human Beta Cell Maturation and Diabetes Pathogenesis](https://npod.org/current-npod-projects/growth-and-development-of-the-endocrine-pancreas-in-humans-extrapolating-normal-physiology-to-the-regenerative-capacity-of-the-endocrine-compartment-in-type-1-and-type-2-diabetes/) - ORIGINAL: Chromogranin A is a global marker of endocrine cells. Most endocrine cells in the pancreas, in addition to Chromogranin A, also express other hormones, Insulin and Glucagon being most frequent, with Pancreatic Polypeptide, Somatostatin and Ghrelin in smaller numbers of endocrine cells. We have found that in adult human pancreas a small number of
- [Pancreatic GLP-1 vs glucagon production in healthy and diabetic patients](https://npod.org/current-npod-projects/pancreatic-glp-1-vs-glucagon-production-in-healthy-and-diabetic-patients/) - Glucagon-like Peptide 1 (GLP-1) and glucagon shares the same precursor molecule proglucagon, but each arises from a distinct posttranslational process in a tissue-specific manner. Recently GLP-1 has been shown to be co-expressed with glucagon in pancreatic islet cells. Our preliminary data showed GLP-1 was progressively up-regulated in pancreatic islets during type 2 diabetes development. These
- [Analysis of Islet Enriched Transcription Factor Expression and Activity in Normal & Type 2 Diabetic Islets](https://npod.org/current-npod-projects/analysis-of-islet-enriched-transcription-factor-expression-and-activity-in-normal-type-2-diabetic-islets/) - The therapeutic treatment of type 1 and type 2 diabetes mellitus (T1DM, T2DM) has vastly improved over the past decades; however complete normalization of glucose remains elusive without proper functioning pancreatic β cells. Truly, sustained and effective treatment of T1DM and T2DM must involve retaining/replacing the function of pancreatic β cells. To this end, we
- [Molecular mechanisms of iron uptake by pancreatic beta cells and their contribution to the development of diabetes](https://npod.org/current-npod-projects/molecular-mechanisms-of-iron-uptake-by-pancreatic-beta-cells-and-their-contribution-to-the-development-of-diabetes/) - Human disease states that result in excess iron accumulation, e.g., hereditary hemochromatosis and beta thalassemia major, are associated with an increased incidence of diabetes. In these disorders surplus iron overwhelms normal transport and storage mechanisms leading to the appearance of excess free iron in circulation and the subsequent deposition of iron in tissues such as
- [Role of Calcium-Independent Phospholipase A2β (iPLA2β) on β-Cell Apoptosis](https://npod.org/current-npod-projects/role-of-calcium-independent-phospholipase-a2-beta-ipla2-beta-on-beta-cell-apoptosis/) - Beta-cell apoptosis contributes to loss of β-cells and decreases in β-cell function in both types 1 and 2diabetes mellitus. It is therefore important to understand the mechanisms underlying β -cell apoptosis if this process is to be prevented or delayed. Our hypothesis is that the group VIA Ca2+-independent phospholipase A2 (iPLA2 β) participates in β
- [Confirmation of target gene expression in T1D and T2D pancreas](https://npod.org/current-npod-projects/confirmation-of-target-gene-expression-in-t1d-and-t2d-pancreas/) - Characterization of expression of novel anti-diabetes targets in pancreas of Type 1 and Type 2 diabetics.
- [Beta Cell Senescence and T1D](https://npod.org/current-npod-projects/beta-cell-senescence-and-t1d/) - Type 1 diabetes (T1D) results from an autoimmune disorder characterized by T-cell-mediated destruction of pancreatic beta cells. While the role of auto-reactive T cells in targeting beta cells has been well defined, the pathological mechanisms operating in beta cells remains poorly understood. To investigate beta cell stress responses during disease progression but before diabetes onset,
- [Deep Learning-based Analyses of Pancreatic Islet Beta-Cell Heterogeneity and MRI Pancreas Volume as Biomarkers to Improve Understanding of Type 1 Diabetes Progression](https://npod.org/current-npod-projects/deep-learning-based-analyses-of-pancreatic-islet-beta-cell-heterogeneity-and-mri-pancreas-volume-as-biomarkers-to-improve-understanding-of-type-1-diabetes-progression/) - The overall objective of this project is to develop a deep learning based imaging analysis and informatics tool for better understanding of pathogenic mechanisms in T1D, using existing data which has been collected for 14 years by Network for Pancreatic Organ Donors with Diabetes (nPOD) study. The central hypothesis of this proposal is that modifications
- [To identify T1D epitope using highly specific autoantibodies](https://npod.org/current-npod-projects/to-identify-t1d-epitope-using-highly-specific-autoantibodies-2/) - DQ8/InsB:R3 was identified as an antigen by functional analysis of reactive T cells from peripheral blood, but direct evidence of antigenicity in islets is missing. The hypothesis is that the endogenous DQ8/InsB:R3 appear at the core organs of T1D in a disease stage-related timing. CODEX is a highly multiplexed tissue imaging platform enabling >50 markers
- [Therapeutic targeting of senescent β cells in Type 1 Diabetes](https://npod.org/current-npod-projects/therapeutic-targeting-of-s-enescen-t-β-cell-s-in-type-1-diabetes/) - A growing body of evidence has implicated various forms of beta cell dysfunction in the pathogenesis of Type 1 Diabetes (T1D). Recently we discovered that during the natural history of T1D, a subpopulation of β cells become senescent. Senescence is a cell type-specific form of permanent growth arrest, which occurs during aging, tissue damage or
- [Evaluating senescence induction in human beta cells upon transplantation](https://npod.org/current-npod-projects/evaluating-senescence-induction-in-human-beta-cells-upon-transplantation/) - We are interested in cell replacement therapy as a potential practical cure for patients suffering from diabetes. Using stem cell derived beta cell clusters and cadaveric human islets transplantation in preclinical animal models we find distinct changes to the beta cell phenotype. This project will test if our observations hold true in islet recipients. We
- [Identification and characterization of Coxsackievirus-reactive CD8+ T cells in pancreatic lymph nodes](https://npod.org/current-npod-projects/identification-and-characterization-of-coxsackievirus-reactive-cd8-t-cells-in-pancreatic-lymph-nodes/) - Despite accumulating epidemiological and histopathological evidence for an association between Coxsackievirus B (CVB) and type 1 diabetes (T1D), a causal link is missing. Very little is known about the T-cell response mounted against CVB infection and the epitopes targeted. Moreover, the ongoing CVB vaccination trials require: 1) the development of T-cell biomarkers to follow vaccination
- [2022 nPOD Annual Scientific Meeting](https://npod.org/news-events/2022-npod-annual-scientific-meeting/) - THANK YOU FOR ATTENDING The Network for Pancreatic Organ donors with Diabetes (nPOD) hosted its 14th Annual Meeting from January 30 - February 2, 2022 at the One Ocean Resort & Spa.
- [COVID-19, Diabetes, and nPOD](https://npod.org/news-events/covid-19-diabetes-and-npod/) - The COVID-19 pandemic has impacted all aspects of life, worldwide. At nPOD, we essentially never ceased operations in terms of donor recoveries. With testing of nPOD donors for Sars-Cov-2, we received 304 offers and nPOD received tissues from 21 cases over the last year (including 3 AAb+ and 6 donors with T1D, of whom two
- [Welcome to new nPOD Staff](https://npod.org/news-events/welcome-to-new-npod-staff/) - Please join us in welcoming Jayne Moraski to the Administrative Core of the nPOD project as assistant director. Jayne comes to us with a background in business and grant management. She has managed state, federal and private foundation grants in excess of @2.5 million annually. Jayne holds a master's degree from the University of Iowa
- [High resolution HLA typing](https://npod.org/current-npod-projects/archived-projects/high-resolution-hla-typing/) - The Noble lab produces HLA genotyping for all nPOD samples.
- [ImmTAAI technology and its potential use to treat autoimmunity](https://npod.org/current-npod-projects/immtaai-technology-and-its-potential-use-to-treat-autoimmunity/) - We propose an industry collaboration between investigators in the University of Florida Diabetes Institute and an industry partner, Immunocore Ltd. Immunocore utilizes its T cell receptor (TCR) technology to generate tissue-targeted immune suppressant bispecifics called ImmTAAI (Immune modulating monoclonal TCR Against Autoimmune Disease) molecules. ImmTAAIs consist of a high affinity TCR-based targeting domain specific for
- [Macrophages in Type 1 Diabetes](https://npod.org/npod-webinars/macrophages-in-type-1-diabetes/) - DATE: Thursday, December 10th, 2020 TIME: 11:00am - 01:00pm EST Download Meeting Recording: Dropbox link to view meeting recording Discussion led by: Hubert Tse, PhD, Joana Collares Pereira Almaca, PhD, Xiangwei Xiao, MD, PhD, and Bruce Verchere, PhD
- [nPOD Pancreas Slices Group: Islet Biology Part 2](https://npod.org/npod-webinars/npod-pancreas-slices-group-islet-biology-part-2/) - DATE: Wednesday, July 14th, 2021 TIME: 11:00am - 01:00pm EST A webinar session to discuss emerging results from nPOD investigators: Ricardo Pastori/Juan Dominguez-Bendala Richard Benninger Emily Sims and the Pro-Hormone Working Group Hubert Tse/Jared Taylor Matthias von Herrath/Mehdi Benkahla
- [Innate Immunity in Type 1 Diabetes in nPOD Donors](https://npod.org/npod-webinars/innate-immunity-in-type-1-diabetes-in-npod-donors/) - DATE: Thursday, July 22nd, 2021 TIME: 11:00am - 01:00pm EST Download Meeting Recording: Dropbox link to view meeting recording Discussion led by: Alberto Pugliese, MD, Sally Kent, PhD, Hubert Tse, PhD, Bruce Verchere, PhD, Xiangwei Xiao, MD, PhD and Rachel Friedman, PhD Last December, we held a stimulating webinar on the role of macrophages in type 1 diabetes.
- [nPOD Pancreas Slices Group Immunology](https://npod.org/npod-webinars/npod-pancreas-slices-group-immunology/) - DATE: Friday, July 16th, 2021 TIME: 11:00am - 01:00pm EST Discussion led by: Irina Kusmartseva, PhD, Maria Beery, MS, and Helmut Hiller, MS
- [nPOD Pancreas Slices Group: Islet Biology Part 1](https://npod.org/npod-webinars/npod-pancreas-slices-group-islet-biology-part-1/) - DATE: Thursday, July 8th, 2021 TIME: 11:00am - 01:00pm EST Discussion led by: Irina Kusmartseva, PhD, Maria Beery, MS, and Helmut Hiller, MS
- [nPOD Pancreas Slice Group Discussion](https://npod.org/npod-webinars/npod-pancreas-slice-group-discussion/) - DATE: Wednesday, May 22nd, 2019 WEBINAR INFO: Click here to join WebEx Meeting Meeting Number (access code): 737 898 840 Meeting Password: xhMMM3CM Call-in toll number (US/Canada): 1-650-479-3208 Global Call-in Numbers Can't join the meeting? TIME: 11:00am - 02:00pm EST Discussion led by: Alberto Pugliese, MD
- [Characterising the organisation of the gut associated lymphoid tissue in type 1 diabetes and tracking T and B cell receptor clonotype / isotype usage and islet antigen-specific lymphocytes across tissues in the nPOD resource.](https://npod.org/current-npod-projects/characterising-the-organisation-of-the-gut-associated-lymphoid-tissue-in-type-1-diabetes-and-tracking-t-and-b-cell-receptor-clonotype-isotype-usage-and-islet-antigen-specific-lymphocytes-across-tiss/) - The intestinal microbiome plays an essential role in host health, where microbial communities have immune-modulating activity and impact on regulatory immune cell compartmentalisation in the gut associated lymphoid tissues. Increasing evidence points to a dysfunctional relationship between the microbiome and host tissues in individuals who develop type 1 diabetes (T1D). Alterations in gut microbial composition
- [2021 nPOD Annual Meeting](https://npod.org/news-events/2021-npod-annual-meeting/) - REGISTER TODAY JOIN US FOR AN INTERACTIVE VIRTUAL SCIENTIFIC SESSION DOWNLOAD MEETING AGENDA **Please Note: Zoom Meeting links are NOT active at this time. Details about access to the virtual meeting and logistics will be communicated soon** The Network for Pancreatic Organ donors with Diabetes (nPOD) will host its 13th Annual Meeting as a virtual
- [Functional Role of CADM1 during Type 1 Diabetes Pathogenesis](https://npod.org/current-npod-projects/functional-role-of-cadm1-during-type-1-diabetes-pathogenesis/) - Type 1 diabetes (T1D) is characterized by the hyperglycemia resulting from the autoimmune destruction of the insulin-expressing β-cells of the pancreas. As the prevalence of the disease continues to increase worldwide, it remains imperative to identify therapeutic strategies that can (1) preserve pancreatic beta-cell mass and function and (2) prevent the immune response that initiates
- [ORIGINAL: Proinsulin processing and presentation in human type 1 diabetes: a beta cell perspective ADDENDUM: Proinsulin processing and presentation in human type 1 diabetes: a beta cell perspective –T cell perspective](https://npod.org/current-npod-projects/proinsulin-processing-and-presentation-in-human-type-1-diabetes-a-beta-cell-perspective/) - ORIGINAL: Type 1 diabetes (T1D) is an autoimmune disease in which clinical symptoms arise as a result of insulin deficiency. While genetic and environmental factors contribute to the disease, in recent years it has become increasingly evident that beta cells might be contributing to their own destruction and might have an active role in type
- [Investigating the role of aggregation-prone proteins in pancreatic beta cell function and health](https://npod.org/current-npod-projects/investigating-the-role-of-aggregation-prone-proteins-in-pancreatic-beta-cell-function-and-health/) - Almost 20% of the chromosomal regions associated with the risk of developing of Type 1 diabetes (T1D) identified to date,have no identified candidate causal gene. Often, this is because the genes in those regions have no known roles in the immune system, reflecting the classical view that T1D is caused by the autoimmune destruction of
- [Analysis of major digestive enzymes expression in human pancreas](https://npod.org/current-npod-projects/analysis-of-major-digestive-enzymes-expression-in-human-pancreas/) - Type I diabetes is characterized by significant loss of beta cells and decrease in pancreatic weight. It is widely accepted that decline of pancreatic weight is attributed to acinar atrophy which in turn could lead to pancreatic exocrine insufficiency frequently found in diabetic patients. However, the fact that the exocrine pancreas is affected in patients
- [ORIGINAL: Immunohistochemical identification of molecular markers of oxidative and nitrosative stress in β-cells during the early stages of human type 1 diabetes mellitus ADDENDUM: Beta cell stress as an early preceding phase of autoimmune type 1 diabetes (T1D): Immunohistochemical assessment of markers of early beta cell vulnerability in newly-diagnosed and long-term diabetic cases](https://npod.org/current-npod-projects/immunohistochemical-identification-of-molecular-markers-of-oxidative-and-nitrosative-stress-in-beta-cells-during-the-early-stages-of-human-type-1-diabetes-mellitus/) - ORIGINAL: This research seeks immunohistochemical evidence of beta cell oxidative and nitrosative stress during various stages of T1D and whether such stressors are also present preceding and during the early onset of the disease. Antibodies to nitrotyrosine, a marker of peroxynitrite-mediated cell damage and to 8-hydroxy-2-deoxyguanosine (a marker of DNA oxidation) are being employed in
- [Transcription factor:coregulator interactions are compromised in islet cells under diabetogenic conditions](https://npod.org/current-npod-projects/transcription-factorcoregulator-interactions-are-compromised-in-islet-cells-under-diabetogenic-conditions/) - Beta cell dysfunction occurs in the early stages of type 1 (T1D) and type 2 diabetes (T2D) development. Strategies to improve outcomes for the mounting number of diabetic patients requires understanding the complex programs that coordinate a proper insulin release in response to changing blood glucose levels and how these mechanisms falter in diabetogenic settings.
- [Evaluation of Fatty Acid Levels in Human Pancreatic Tissue using Imaging Mass Spectrometry](https://npod.org/current-npod-projects/evaluation-of-fatty-acid-levels-in-human-pancreatic-tissue-using-imaging-mass-spectrometry/) - Alterations in metabolism are known to play profound roles inthe health ofindividuals with type 1 diabetes (T1D). These alterations lead to disruptions in cell structure, signaling, and energy homeostasis. While most studies focus on identifying genetic or protein signatures of disease, the metabolic profile is a far more dynamic, sensitive, and rapidly-responding molecular measure of
- [Conversion of human acinar cells into insulin - producing cells](https://npod.org/current-npod-projects/conversion-of-human-acinar-cells-into-insulin-producing-cells/) - Reprogramming of acinar cells toward functional β-like cells would offer an abundant and autologous source of insulin-producing cells. The current literature suggeststhat manipulation of genetic factors,in combination with cytokines or small molecules targeting specific pathways and the epigenetic machinery in humans,may eventually yield functional acinar-derived β-like cells. Our preliminary datashowthat treatment of adult mice with
- [CD3+CD56+ T lymphocytes, as novel T1D biomarker](https://npod.org/current-npod-projects/cd3cd56-t-lymphocytes-as-novel-t1d-biomarker/) - Type 1 Diabetes (T1D) is a disease characterized by a lymphocyte-mediated destruction of insulin-producing beta-cells in genetically susceptible individuals. It has been demonstrated that lymphocytes with regulatory properties are essential for the maintenance of tolerance against self-tissues (pancreas) and alterations in the number and functions of these cells have been correlated with the break of
- [nPOD Slice Manuscript Publication](https://npod.org/news-events/npod-slice-manuscript-publication/) - Pancreas tissue slices from organ donors enable in situ analysis of type 1 diabetes pathogenesis. Panzer JK, Hiller H, Cohrs CM, Almaça J, Enos SJ, Beery M, Cechin S, Drotar DM, Weitz JR, Santini J, Huber MK, Muhammad Fahd Qadir M, Pastori RL, Domínguez-Bendala J, Phelps EA, Atkinson MA, Pugliese A, Caicedo A, Kusmartseva I,
- [Regulation of Islet Autophagy in Type 1 Diabetes](https://npod.org/current-npod-projects/regulation-of-islet-autophagy-in-type-1-diabetes/) - Oxidative stress occurs when increased production of cellular oxidants (e.g. Reactive Oxygen Species, or ROS) is not compensated for by an increase in cellular antioxidants/antioxidant enzymes. A considerable body of evidence supports the conclusion that oxidative stress is a common feature of type 1 diabetes (T1D), and that the process of autophagy plays a key
- [The cellular distribution of Golli protein in human pancreatic islets](https://npod.org/current-npod-projects/the-cellular-distribution-of-golli-protein-in-human-pancreatic-islets-2/) - The objective of this application is to determine the cellular distribution of Golli protein in human pancreatic islets. Golli (gene expressed in oligodendrocyte lineage) is an alternatively spliced product of myelin basic protein (MBP) gene featured by the presence of a unique Golli domain which contains a 133-amino-acid sequence fused in the N-terminal of classic
- [Modulation of autoimmune diabetes by natural antibodies specific for N-acetyl-D- glucosamine](https://npod.org/current-npod-projects/modulation-of-autoimmune-diabetes-by-natural-antibodies-specific-for-n-acetyl-d-glucosamine/) - The focus of our research project is to improve the understanding of how antigenic stimulation of the B lymphocyte repertoire development during early life may protect from Type 1 Diabetes (T1D). Specifically, we are investigating how exposure to the common pathogen Group A Streptococcus promotes the formation of a T1D-suppressive natural antibody repertoire. Exposure to
- [Dissecting the role intestinal NK cells play in T1D pathogenesis](https://npod.org/current-npod-projects/dissecting-the-role-intestinal-nk-cells-play-in-t1d-pathogenesis/) - There is conflicting information regarding the role of natural killer (NK) cells in the development of Type 1 Diabetes (T1D). NK cells represent an important effector arm against viral infection, and mounting evidence suggests that viral infection plays a role in the development of T1D in at least a portion of patients. NK cells of
- [Persistence of Pancreatic Insulin mRNA Expression and Proinsulin Protein and the Autoantigens GAD, IA-2 and ZnT8 in Type 1 Diabetes Pancreata](https://npod.org/current-npod-projects/persistence-of-pancreatic-insulin-mrna-expression-and-proinsulin-protein-and-the-autoantigens-gad-ia-2-and-znt8-in-type-1-diabetes-pancreata/) - The canonical notion that type 1 diabetes (T1D) results following a complete destruction of β cells has recently been questioned as small amounts of C-peptide are detectable in patients with long-standing disease. Previously, we analyzed protein and gene expression levels for proinsulin, insulin, C-peptide, and islet amyloid polypeptide within pancreatic tissues from T1D, autoantibody positive
- [Role of Rab1A in Diabetes Mellitus](https://npod.org/current-npod-projects/role-of-rab1a-in-diabetes-mellitus/) - We previously identified the Rab1A small GTPase as a conserved activator of mTORC1 in response to amino acid (AA) stimulation in yeast and mammalian cells. To better understand the physiological function of Rab1A- mTORC1 signaling in vivo, we genetically engineered conditional Rab1A knockout (Rab1A-KO) in mice. We found that tamoxifen-induced Rab1A knockout in young adult
- [Harnessing the power of beta cell regeneration to treat diabetes](https://npod.org/current-npod-projects/harnessing-the-power-of-beta-cell-regeneration-to-treat-diabetes/) - Approximately 1 in 11 people worldwide is afflicted by diabetes, a disease caused by a shortage of beta cells. The current standard of care for patients with severe diabetes is insulin therapy or pancreatic islet transplantation. However, insulin therapy requires frequent insulin injection, detailed dose calibration and close monitoring of blood glucose; for islet transplantation,
- [Highly Multiplexed Imaging Mass Cytometry of the Human Pancreas Affected by Type 1 Diabetes](https://npod.org/current-npod-projects/highly-multiplexed-imaging-mass-cytometry-of-the-human-pancreas-affected-by-type-1-diabetes/) - Despite extensive efforts, understanding of the mechanisms of type 1 diabetes (T1D) development in human patients is collectively lacking, particularly with regard to contributions of islet endocrine cells, the immune system, and their interplay in the disorder’s development. Until recently, these questions have been particularly challenging to address due to technical limitations in the number
- [2020 nPOD Annual Meeting](https://npod.org/news-events/2020-npod-annual-meeting/) - The Renaissance Tampa International Plaza Hotel 4200 Jim Walter Blvd, Tampa, FL 33607 DOWNLOAD MEETING AGENDA The Network for Pancreatic Organ donors with Diabetes (nPOD) is proud to host its 12th Annual Meeting at the Renaissance Tampa International Plaza Hotel in Tampa, Florida, from February 23-26, 2020. Over the past twelve years, nPOD has made remarkable strides in terms
- [NIH RFA for T1D Research](https://npod.org/news-events/nih-rfa-for-t1d-research/) - NIH RFA 19-026: New Investigator Gateway Awards for Collaborative T1D Research ** Letter of Intent Due Date(s): February 25, 2020 & October 3, 2020 ** NIH has issued a “New Investigator Gateway Awards for Collaborative T1D Research” Funding Initiative. This initiative is similar to our internal HIRN New Investigator Initiative we held a few years ago
- [Call for Abstract](https://npod.org/news-events/call-for-abstract/) - nPOD 12th Annual Scientific Meeting SEND IN YOUR LATE BREAKING ABSTRACT There is much anticipation for this event and we encourage each nPOD Investigator to submit abstracts of research utilizing nPOD resources. Deadline for late breaking abstract submission is Monday, December 2, 2019. Please use this template to prepare your abstract(s) and email them to Monica Novak.
- [Recruiting Principal Investigators at the IBRI Diabetes Center/Lilly Diabetes Center of Excellence](https://npod.org/news-events/recruiting-principal-investigators-at-the-ibri-diabetes-centerlilly-diabetes-center-of-excellence/) - The Lilly Diabetes Center of Excellence (LDCE) at the Indiana Biosciences Research Institute (IBRI) is recruiting promising young investigators to start their own independent laboratories under excellent research conditions The LDCE is recruiting high-potential Principal Investigators (PIs) at the IBRI in Indianapolis, Ind., who are interested in pancreatic beta cell research, particularly related to iPSC-derived
- [T1D BBQ!](https://npod.org/news-events/t1d-bbq/) - T1D BBQ! Sally Kent and Alberto Pugliese initiated and hosted the T1D BBQ (Big Burning Questions) webinar for the nPOD Autoimmunity T Cell Working Group with Francesco Dotta, Dale Greiner, Bart Roep, and Matthias von Herrath as Discussants. The purpose of this webinar was to discuss ‘T cells as pathogenic effectors in human type 1
- [Role of Monogenic Diabetes, Integrated Stress Response, mTOR and NR4A genes in Type 1 Diabetes](https://npod.org/current-npod-projects/role-of-monogenic-diabetes-integrated-stress-response-mtor-and-nr4a-genes-in-type-1-diabetes/) - To date, over 60 loci have been identified, including the HLA and insulin regions and various single nucleotide polymorphisms (SNPs), conferring genetic susceptibility for type 1 diabetes (T1D); however, a number of these loci do not correspond to specific genes and as such, do not infer disease mechanisms or etiology. We have taken an innovative
- [Human T cell repertoire profiling in Type 1 Diabetes](https://npod.org/current-npod-projects/human-t-cell-repertoire-profiling-in-type-1-diabetes/) - Current strategies of studying islet autoantigen-specific T cells rely heavily on fluorescence labeled tetramers and are limited in the throughput of antigens that can be analyzed simultaneously. The profile of islet autoantigen-specific T cells that can be obtained is limited. We recently developed a new technology, Tetramer associated TCR sequencing (TetTCR-Seq), that is capable of
- [Analysis of cellular proteins in β cells from patients with type 1 diabetes and healthy](https://npod.org/current-npod-projects/analysis-of-cellular-proteins-in-β-cells-from-patients-with-type-1-diabetes-and-healthy/) - Our objective is to identify characteristics of β cells that survive immune attack. Our hypothesis is that these residual cells express immune inhibitory molecules and/or decrease expression of diabetes associated antigens. In addition, these cells may show changes consistent with dedifferentiated β cells. Our studies of protein modifications have also shown that there are post-translational
- [The role of protein kinase C delta in beta-cell death and dysfunction in type 1 diabetes](https://npod.org/current-npod-projects/the-role-of-protein-kinase-c-delta-in-beta-cell-death-and-dysfunction-in-type-1-diabetes/) - The goal of this study is identify novel mechanisms of immune-mediated β-cell death and dysfunction such that these mechanisms can be exploited to protect against the onset and progression of T1D. To achieve this goal, we propose the following aims: 1) Determine if reduced electrical coupling, altered calcium signaling, and activation of protein kinase C
- [Mechanisms and dynamics of GAD and GABA in human beta cells](https://npod.org/current-npod-projects/how-does-induction-of-er-stress-in-pancreatic-beta-cells-affect-the-processing-and-trafficking-of-beta-cell-autoantigens/) - In humans, GAD65 is one of the first targets of autoimmunity and 70-80% of human diabetics have antibodies against this protein. In late-onset patients, circulating GAD65 autoantibodies may even be more predicative of future disease onset than antibodies to insulin. The early appearance of GAD65 antibodies may hold a clue that GAD65 is implicated during
- [nPOD-Virus Group Slice RFA Webinar](https://npod.org/npod-webinars/slice-rfa-npod-virus-group/) - DATE: Tuesday, April 23rd, 2019 Download Meeting Recording: Dropbox link to view meeting recording Discussion led by: Alberto Pugliese, MD and Sarah Richardson, PhD
- [nPOD-Autoimmunity Group Slice RFA Webinar](https://npod.org/npod-webinars/npod-autoimmunity-group-slice-rfa-webinar/) - DATE: Wednesday, April 24th, 2019 Download Meeting Recording: Dropbox link to view meeting recording Discussion led by: Alberto Pugliese, MD and Sally Kent, PhD
- [nPOD-Extracellular Matrix Group Slice RFA Webinar](https://npod.org/npod-webinars/npod-extracellular-matrix-group-slice-rfa-webinar/) - DATE: Thursday, April 25th, 2019 Download Meeting Recording: Dropbox link to view meeting recording Discussion led by: Alberto Pugliese, MD and Marika Bogdani, PhD
- [nPOD Islet Dysfunction Group and Exocrine Function Group Slice RFA Webinar](https://npod.org/npod-webinars/npod-islet-dysfunction-group-and-exocrine-function-group-slice-rfa-webinar/) - DATE: Friday, April 26th, 2019 Download Meeting Recording: Dropbox link to view meeting recording Discussion led by: Stefan Speier, PhD and Clayton Mathews, PhD
- [Examination of ER stress markers in type 1 diabetes samples in humans](https://npod.org/current-npod-projects/examination-of-er-stress-markers-in-type-1-diabetes-samples-in-humans/) - The endoplasmic reticulum (ER) is an organelle that is responsible for the proper folding of proteins and biosynthesis of lipids and steroids. Disruption of ER homeostasis leads to ER stress and activates a highly conserved adaptive network called the unfolded protein response (UPR). ER stress has been implicated in several diseases associated with protein folding
- [10th Annual OPO Workshop](https://npod.org/news-events/10th-annual-opo-workshop/) - nPOD 10th Annual OPO Workshop This year's workshop will be held in beautiful St. Pete Beach, Florida on October 22-24, at the TradeWinds Islands Grand Resort. Registration will be free! Continuing education credits, hotel room, meals and entertainment are complimentary. Attendees are only responsible for their travel to and from the workshop. nPOD relies on your research organ referrals
- [2017 nPOD 9th Annual Scientific Meeting - Speakers & Abstracts](https://npod.org/news-events/2017-jdrf-npod-8th-annual-scientific-meeting-speakers/) - Announcing Speakers & Call for Abstracts We are both pleased and honored to announce that the following speakers have already been confirmed for the 2017 nPOD Annual Scientific Meeting, which will be held February 19-22, 2017 at the Fort Lauderdale Marriott Harbor Beach Resort & Spa. We also look forward to hearing from you, our
- [Monogenic Cases Now Identified in the nPOD Repository](https://npod.org/uncategorized/monogenic-cases-now-identified-in-the-npod-repository/) - Genetic testing leads to new case classification in DataShare Advancing technologies in the area of Next-Generation DNA Sequencing have revolutionized the study of genomics and molecular biology. The great majority of patients with monogenic diabetes would never be diagnosed without such gene testing and, as a result, are usually mis-diagnosed as having Type 1 or Type
- [Generating Pancreas Slices for Organotypic Culture and Functional Assessments](https://npod.org/news-events/generating-pancreas-slices-for-organotypic-culture-and-functional-assessments/) - Dr. Stephan Speier has recently established a novel technique for generating pancreas tissue slices, which are suitable for conducting functional assessments of beta cells without the need for and the downsides of islet isolation (Marciniak et al, Nat Protoc, 2014). This technique has been developed in rodent pancreas, and Dr. Speier had conducted limited experimentation
- ["Clinical Images" in Diabetes Care](https://npod.org/news-events/clinical-images/) - Invitation to Submit Reviews of nPOD Cases in "Clinical Images" in Diabetes Care In their July 2016 issue, Diabetes Care introduced a new manuscript category called “Clinical Images in Diabetes”. As described in the Editor’s Commentary, this series is intended to “provide modern pictorial views on the pathogenesis of diabetes or its complications, with the aim
- [nPOD Call for Co-Executive Director](https://npod.org/news-events/npod-call-for-co-executive-director/) - JDRF-nPOD CALL FOR CO-EXCUTIVE DIRECTOR Preparing for the Next Era of nPOD Research To the Type 1 Diabetes Research and Care Community: We are pleased to announce the initiation of an open search for a new Co-Executive Director to join our leadership team at the Network for Pancreatic Organ donors with Diabetes (nPOD). Our hope
- [nPOD Islet Isolation Program (IIP)](https://npod.org/news-events/npod-islet-isolation-program-iip/) - Introducing the New nPOD Islet Isolation Program (IIP) & Planning Webinar Dear Colleagues and nPOD Investigators: You may be aware that nPOD has recently received renewed financial support from the Breakthrough T1D and the Helmsley Charitable Trust, and will continue to recover tissues from organ donors in support of T1D research. As part of this
- [nPOD Case Deep Dive: #6243](https://npod.org/npod-webinars/npod-case-deep-dive-6243-2/) - DATE: Tuesday, October 24th, 2017 SPEAKERS: nPOD Investigators, group discussion led by Alberto Puglese, MD Click here to view a recording of the webinar. The nPOD “Deep Dive” Case Study webinar series aims to unite the efforts, expertise, and resources of various Investigators within the nPOD cohort in order to paint a uniquely detailed
- [2018 Publications (9)](https://npod.org/publications/2018-publications-3/) - Richardson SJ, Morgan NG. (2018) Enteroviral infections in the pathogenesis of type 1 diabetes: new insights for therapeutic intervention. Curr Opin Pharmacol. 2018 Jul 28;43:11-19. doi: 10.1016/j.coph.2018.07.006. Ifie E, Russell MA, Dhayal S, Leete P, Sebastiani G, Nigi L, Dotta F, Marjomäki V, Eizirik DL, Morgan NG, Richardson SJ. (2018) Unexpected subcellular distribution of a
- [Genetic and Epigenetic Fine-Mapping of Causal Type 1 Diabetes Risk Variants](https://npod.org/npod-webinars/genetic-and-epigenetic-fine-mapping-of-causal-type-1-diabetes-risk-variants-3/) - DATE: Tuesday, April 26th, 2016 SPEAKER: Alex Marson, MD, PhD, University of California, San Francisco Click here to view a recording of the webinar. Type 1 diabetes (T1D) has been the subject of intensive genetic investigations, including genome-wide association studies (GWAS) that have collectively identified over 40 risk loci in the genome. Our knowledge of
- [Immunosequencing: Generating a Potential New Class of Diagnostics](https://npod.org/npod-webinars/immunosequencing-generating-a-potential-new-class-of-diagnostics/) - DATE: Tuesday, December 15th, 2015 SPEAKER: Erik Yusko, PhD, Adaptive Biotechnologies Todd Brusko, PhD, University of Florida Click here to view a recording of the webinar. The cellular and humoral adaptive immune system generates a remarkable breadth of diversity of antigen receptors by combinatorial shuffling of gene segments in somatic cells. The diversity of possible receptors
- [Venue for Highlighting Unique nPOD Cases: Clinical Images in Diabetes](https://npod.org/npod-webinars/venue-for-highlighting-unique-npod-cases-clinical-images-in-diabetes/) - DATE: Tuesday, July 26th, 2016 SPEAKER: Laura Jacobsen, MD University of Florida Click here to view a recording of the webinar. The nPOD database offers a wealth of knowledge; consolidating a large series of cases and slides into a collective that provides valuable data towards common features for the disorder’s pathogenesis. However, extensive information also
- [Islet-Infiltrating T Cell Repertoire: Two Studies](https://npod.org/npod-webinars/dual-webinar-t-cell-repertoire-cd4-t-cells-targeting-proinsulin/) - DATE: Wednesday, January 11th, 2017 SPEAKERS: Sally Kent, PhD University of Massachusetts Medical School Maki Nakayama, MD, PhD University of Colorado Click here to view a recording of the webinar. Broad Repertoire of Autoreactive T Cells from the Islets of Donors with Type 1 Diabetes: In this webinar, we will be discussing our recent study on the
- [Tissue Distribution and Clonal Diversity](https://npod.org/news-events/analysis-of-self-antigen-specificity-2/) - Tissue Distribution and Clonal Diversity Autoreactive CD4+ and CD8+ T cells have been identified from the peripheral blood and islets of type 1 diabetes (T1D) patients and organ donors, and autoantibodies secreted by autoreactive B cells are the most widely used biomarker of T1D. However, the T1D adaptive immune repertoire had not been examined without
- [Analysis of Self Antigen Specificity](https://npod.org/news-events/analysis-of-self-antigen-specificity/) - Self-Antigen Specificity of Islet-Infiltrating T-cells Autoreactive T cells specific for beta cell antigens have previously been identified from the peripheral blood of type 1 diabetes (T1D) patients, and immune cells within the pancreatic insulitis lesion been immunophenotyped from human organ donor tissues. However, this report by Babon et al. represents one of the first and
- [6 Kid Map](https://npod.org/home-slideshow/2/) - Our scientists are not locatedin one lab,on one campus,or in one country...
- [5 Kid Back](https://npod.org/home-slideshow/1/) - One Life One Gift One Hope
- [Welcome Dr. Carmella Evans-Molina](https://npod.org/news-events/welcome-dr-carmella-evans-molina/) - Welcome Dr. Carmella Evans-Molina New nPOD Co-Executive Director We are so honored and happy to announce that Carmella Evans-Molina, MD, PhD has joined the Network for Pancreatic Organ donors with Diabetes (nPOD) program to serve as a Co-Executive Director and be part of the leadership team. Carmella is a physician scientist with strong type 1
- [3 Girl United](https://npod.org/home-slideshow/3/) - We are united in one goal, to cure type 1 diabetes.
- [Pancreatic Islet Cell Hyperexpression](https://npod.org/news-events/pancreatic-islet-cell-hyperexpression/) - Pancreatic Islet Cell Hyperexpression Pancreatic islet cell hyperexpression of HLA class-I was first observed from a small number of autopsy specimens from type 1 diabetes (T1D) patients in the 1980s, but in recent years, this notion was challenged as a potential artifact. Richardson et al. performed immunohistochemistery, immunofluorescence, and Affymetrix array analysis of RNA isolated
- [2017 Publications (36)](https://npod.org/publications/2017-publications/) - Jackson J, Posgai A, Campbell-Thompson M, Kusmartseva I. (2017) Insulitis in Autoantibody-Positive Pancreatic Donor With History of Gestational Diabetes Mellitus. Diabetes Care. 2017 May;40(5):723-725. doi: 10.2337/dc16-2127. No Abstract Jackson J, Posgai A, Campbell-Thompson M, Kusmartseva I. (2017) Response to Comment on Jackson et al. Insulitis in Autoantibody-Positive Pancreatic Donor With History of Gestational Diabetes Mellitus.
- [2018 nPOD Annual Meeting Agenda](https://npod.org/news-events/2018-npod-annual-meeting-agenda/) - DOWNLOAD COMPLETE MEETING AGENDA
- [2016 Publications (30)](https://npod.org/publications/2016-publications/) - Poudel A, Fowler JL, Zielinski MC, Kilimnik G, Hara M. (2016) Stereological analyses of the whole human pancreas. Sci Rep. 2016 Sep 23;6:34049. doi: 10.1038/srep34049. Maccari G, Genoni AP, Sansonno S, Toniolo A (2016). Properties of two enterovirus antibodies that are utilized in diabetes research. Sci. Rep. 2016; 6, 24757; doi: 10.1038/srep24757. Hackeng WM, Brosens
- [2018 nPOD 10th Annual Scientific Meeting - Registration](https://npod.org/news-events/2018-jdrf-npod-10th-annual-scientific-meeting-registration-2/) - Registration Closed The Network for Pancreatic Organ Donors with Diabetes (nPOD) is proud to host its 10th Annual Meeting at The Diplomat Beach Resort, February 20-23, 2018. Over the past eleven years, nPOD has made remarkable strides in terms of meeting its organizational goals. Key amongst these include seeing its investigators make discoveries that help
- [2018 nPOD 10th Annual Scientific Meeting - Registration](https://npod.org/news-events/2018-jdrf-npod-10th-annual-scientific-meeting-registration/) - Registration Information The Network for Pancreatic Organ Donors with Diabetes (nPOD) is proud to host its 10th Annual Meeting at The Diplomat Beach Resort, February 20-23, 2018. Over the past eleven years, nPOD has made remarkable strides in terms of meeting its organizational goals. Key amongst these include seeing its investigators make discoveries that help
- [2013 Publications (25)](https://npod.org/publications/2013-publications/) - In Print Toniolo A , Salvatoni A, Federico G, Diaz-Horta O, Maccari G, Baj A (2013). Enteroviruses in blood. In: “Diabetes and Viruses”. K Taylor, H Hyoty, A Toniolo, A Zuckerman (Eds.), Springer, New York, 2013; pp. 143-156. Fiaschi-Taesch NM, Kleinberger JW, Salim FG, Troxell R, Wills R, Tanwir M, Casinelli G, Cox AE, Takane
- [2015 Publications (17)](https://npod.org/publications/2015-publications/) - Nagafuchi S, Toniolo A (2015). Viral diabetes: virus diabetogenicity and host susceptibility. Immunoendocrinology 2015; 2: e1026. doi: 10.14800/ie.1026. 1. Ramirez L, Hamad AR (2015). From non-obese diabetic to Network for the Pancreatic Organ Donors with Diabetes: New Heights in type 1 diabetes research. World Journal of Diabetes. 2015 Nov 25;6(16):1309-11. doi: 10.4239/wjd.v6.i16.1309. Kaddis JS, Pugliese A,
- [2010 Publications (4)](https://npod.org/publications/2010-publications/) - In Print Gianani, R., Campbell-Thompson, M., Sarkar, S. A., Wasserfall, C., Pugliese, A., Solis, J. M., Kent, S. C., Hering, B., West, E., Steck, A., Bonner-Weir, S., Atkinson, M., Coppieters, K., von Herrath, M., & Eisenbarth, G. S. (2010). Dimorphic histopathology of long standing childhood-onset diabetes. Diabetologia, 53(4), 690-8. Keenan, H.A., Sun, J.K., Levine, J.,
- [2012 Publications (18)](https://npod.org/publications/2012-publications/) - In Print Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D. (2012) Expression and regulation of chemokines in murine and human type 1 diabetes. Diabetes. 2012 Feb;61(2):436-46. doi: 10.2337/db11-0853. Epub 2011 Dec 30. Evanko SP, Potter-Perigo S, Bollyky PL, Nepom GT, Wight TN. (2012). Hyaluronan and
- [nPOD Co-Executive Director Awarded the J. Enloe and Eugenia J. Dodson Chair in Diabetes Research](https://npod.org/news-events/npod-co-executive-director-awarded-the-j-enloe-and-eugenia-j-dodson-chair-in-diabetes-research/) - Congratulations to Alberto Pugliese, MD, of the University of Miami Miller School of Medicine’s Diabetes Research Institute, and Co-Executive Director of the Network for Pancreatic Organ Donors with Diabetes (nPOD), on this notable award! The endowed chair will help support Dr. Pugliese’s research in the area of immunology/immune tolerance issues related to Type 1 Diabetes. Click
- [2017 nPOD 9th Annual Scientific Meeting - Registration](https://npod.org/news-events/2017-jdrf-npod-8th-annual-scientific-meeting-registration/) - Registration Information The Network for Pancreatic Organ Donors with Diabetes (nPOD) is proud to host its 9th Annual Meeting at the Fort Lauderdale Marriott Harbor Beach Resort & Spa in Fort Lauderdale, Florida, February 19-22, 2017. Over the past nine years, nPOD has made remarkable strides in terms of meeting its organizational goals. Key amongst
- [Imaging Mass Cytometry for the Analysis of the Human Diabetic Pancreas](https://npod.org/current-npod-projects/imaging-mass-cytometry-for-the-analysis-of-the-human-diabetic-pancreas/) - By integrating systematic views of the transcription, translation machinery at single-cell level, we intend to gain insights into the molecular changes in diabetes and in aging and provide the research community with useful database resources.
- [UMMS Researchers Isolate Immune Cells From the Islets of Donors with Type 1 Diabetes](https://npod.org/news-events/umms-researchers-isolate-immune-cells-from-the-islets-of-donors-with-type-1-diabetes/) - Congratulations to our nPOD Investigators on developing the first large bank of islet-infiltrating T cells isolated from the islets of human donors with T1D WORCESTER, MA – In a highly collaborative effort, scientists at the University of Massachusetts Medical School have isolated and characterized a large bank of live islet-infiltrating T cells directly from the
- [Transcriptome analysis of highly purified islet cell populations](https://npod.org/current-npod-projects/transcriptome-analysis-of-highly-purified-islet-cell-populations/) - Therapies focused on sustaining or promoting endogenous insulin production for individuals at risk for or with T1D will require a thorough understanding of the gene expression patterns in islet α, β, and δ cells from control subjects and from individuals with T1D. Toward that end, we have developed methods to essentially purify α, β, and δ cells from human pancreata by
- [March 2016 Investigator Spotlight](https://npod.org/news-events/march2016investigatorspotlight/) - Richard Oram, M.D., Ph.D. NIHR Clinical Lecturer Diabetes and Nephrology at the University of Exeter Medical School and a Research Fellow at the University of Alberta Medical School The JDRF-nPOD Scientists Who Seek the Cure for T1D Dr. Oram studies clinical nephrology and the biology of beta cell decline in type 1 diabetes. Read
- [nPOD Insulitis Case 6243 Featured on Cover of Diabetes](https://npod.org/news-events/insulitiscase6243/) - nPOD Insulitis Case 6243 Featured on Cover of Diabetes Research led by Dr. Martha Campbell-Thompson was featured on the cover of the March 2016 issue of Diabetes. Insulitis in a 13-year-old organ donor with type 1 diabetes for 5 years (nPOD 6243). Immune cell phenotypes in insulitic islets were determined by multi-immunofluoresence. An insulitic
- [nPOD Publications Reach 100](https://npod.org/news-events/npod-publications-reach-100/) - Dr. Martha Campbell-Thompson recently published the 100th nPOD publication: Organ donor specimens: What can they tell us about type 1 diabetes? Pediatric Diabetes 2015:16:320-330. “This mini-review will provide some key histopathological findings emanating from the nPOD collection, including the heterogeneity of beta cell loss and islet inflammation (or insulitis, shown in the image to the
- [January 2016 Investigator Spotlight](https://npod.org/news-events/january-2016-investigator-spotlight/) - Agata Jurczyk Instructor at the University of Massachusetts Medical School in the Diabetes Center of Excellence The JDRF-nPOD Scientists Who Seek the Cure for T1D Dr. Jurczyk's research focuses on the mechanisms of insulin secretion in pancreatic beta cells. Read More
- [December 2015 Investigator Spotlight](https://npod.org/news-events/december-2015-investigator-spotlight/) - Teresa Rodriguez-Calvo Instructor at the La Jolla Institute for Allergy & Immunology The JDRF-nPOD Scientists Who Seek the Cure for T1D Dr. Rodriguez-Calvo is a member of the La Jolla Institute's Developmental Immunology - von Herrath Lab and a part of the nPOD-Viral working group. Read More
- [nPOD Webinar- Immunosequencing: Generating a Potential New Class of Diagnostics](https://npod.org/news-events/npod-webinar-immunosequencing/) - nPOD Webinar- Immunosequencing: Generating a Potential New Class of Diagnostics Date: Tuesday, December 15th, 2015 Time: 11:00AM (EST) - 12:00PM (EST) Speakers: Erik Yusko, PhD, Adaptative Biotechnologies and Todd Brusko, PhD, Universtity of Florida Find more information on the webinar and a link to the meeting here.
- [November 2015 Investigator Spotlight](https://npod.org/news-events/november-2015-investigator-spotlight/) - David Leslie Professor of Diabetes and Autoimmunity and Diabetes Center Lead at the Blizard Institute The JDRF-nPOD Scientists Who Seek the Cure for T1D Dr. Leslie is an nPOD investigator at the Blizard Institute at the University of London. He is also a consultant physician at St. Bartholomew’s Hospital in London. Read More
- [October 2015 Investigator Spotlight](https://npod.org/news-events/october-2015-investigator-spotlight/) - Antonio Toniolo Researcher at the University of Insubria Medical SchoolThe JDRF-nPOD Scientists Who Seek the Cure for T1DDr. Toniolo is an nPOD investigator at the University of Insubria Medical School in Varese, Italy. He studies viruses that may trigger diabetes and is a member of the nPOD-Virus group. Read More
- [September 2015 Investigator Spotlight](https://npod.org/news-events/september-2015-investigator-spotlight/) - Abdu Hamad Associate Professor of Pathology and Medicine at Johns Hopkins University School of MedicineThe JDRF-nPOD Scientists Who Seek the Cure for T1DEach month, we highlight one of the nPOD investigators. Thanks to the fundraising of all the Breakthrough T1D chapters, these scientists are able to have access to nPOD samples for their work. We’ll
- [July 2014 nPOD Investigator Spotlight](https://npod.org/news-events/july-2014-jdrf-npod-research-update/) - Decio Eizirik, MD. D.Med., PhDUniversitet Libre de Bruxelles, Brussels, Belgium The JDRF-nPOD Scientists Who Seek the Cure for T1D Each month, we highlight one of the nPOD investigators. Thanks to the fundraising of all the Breakthrough T1D chapters, these scientists are able to have access to nPOD samples for their work. We’ll let them introduce
- [August 2014 Investigator Spotlight](https://npod.org/news-events/august-2014-investigator-spotlight/) - Margaret A. Morris, PhDAssistant Professor, Eastern Virginia Medical School The JDRF-nPOD Scientists Who Seek the Cure for T1D Each month, we highlight one of the nPOD investigators. Thanks to the fundraising of all the Breakthrough T1D chapters, these scientists are able to have access to nPOD samples for their work. We’ll let them introduce themselves,
- [September 2014 Investigator Spotlight](https://npod.org/news-events/september-2014-investigator-spotlight/) - C. Garrison Fathman, MD Professor of Medicine in the Division of Immunology and Rheumatology Stanford University School of Medicine The JDRF-nPOD Scientists Who Seek the Cure for T1D Each month, we highlight one of the nPOD investigators. Thanks to the fundraising of all the Breakthrough T1D chapters, these scientists are able to have access to
- [October 2014 Investigator Spotlight](https://npod.org/news-events/october-2014-investigator-spotlight/) - Marika Bogdani, MD, PhDResearch Scientist, Benaroya Research Institute The JDRF-nPOD Scientists Who Seek the Cure for T1D Each month, we highlight one of the nPOD investigators. Thanks to the fundraising of all the Breakthrough T1D chapters, these scientists are able to have access to nPOD samples for their work. We’ll let them introduce themselves, tell
- [November 2014 Investigator Spotlight](https://npod.org/news-events/november-2014-investigator-spotlight/) - Richard E. LloydPhD Professor of Molecular Virology and Microbiology at Baylor College of Medicine The JDRF-nPOD Scientists Who Seek the Cure for T1D Each month, we highlight one of the nPOD investigators. Thanks to the fundraising of all the Breakthrough T1D chapters, these scientists are able to have access to nPOD samples for their work.
- [December 2014 Investigator Spotlight](https://npod.org/news-events/december-2014-investigator-spotlight/) - Dr. Charmaine Simeonovic and Professor Chris Parish Dr. Simeonovic is the Team Leader of the Diabetes/Transplantation Immunobiology Laboratory at the John Curtin School of Medical Research and Professor Parish is a Leader of the Cancer and Vascular Biology Group at the JCSMR, Australian National University, Canberra The JDRF-nPOD Scientists Who Seek the Cure for T1D
- [January 2015 Investigator Spotlight](https://npod.org/news-events/january-2015-investigator-spotlight-2/) - Dr. Shannon Wallet, Ph.D. Associate Professor Department of Periodontology University of Florida The JDRF-nPOD Scientists Who Seek the Cure for T1DEach month, we highlight one of the nPOD investigators. Thanks to the fundraising of all the Breakthrough T1D chapters, these scientists are able to have access to nPOD samples for their work. We’ll let them
- [March 2015 Investigator Spotlight](https://npod.org/news-events/march-2015-investigator-spotlight/) - Sally Kent, PhDAssistant Professor, University of Massachusetts Medical SchoolThe JDRF-nPOD Scientists Who Seek the Cure for T1D Each month, we highlight one of the nPOD investigators. Thanks to the fundraising of all the Breakthrough T1D chapters, these scientists are able to have access to nPOD samples for their work. We’ll let them introduce themselves, tell
- [March 2015 Investigator Spotlight](https://npod.org/news-events/march-2015-research-update/) - Matthias von Herrath, MDProfessor, La Jolla Institute; Vice President and Head, Seattle R&D Center NovoNordisk The JDRF-nPOD Scientists Who Seek the Cure for T1D Each month, we highlight one of the nPOD investigators. Thanks to the fundraising of all the Breakthrough T1D chapters, these scientists are able to have access to nPOD samples for their
- [April 2015 Investigator Spotlight](https://npod.org/news-events/april-2015-investigator-spotlight/) - Heikki Hyöty M.D., PhDProfessor at the University of Tampere, Finland The JDRF-nPOD Scientists Who Seek the Cure for T1D Each month, we highlight one of the nPOD investigators. Thanks to the fundraising of all the Breakthrough T1D chapters, these scientists are able to have access to nPOD samples for their work. We’ll let them introduce
- [May 2015 Investigator Spotlight](https://npod.org/news-events/may-2015-investigator-spotlight/) - Lydia SorokinDirector of Physiological Chemistry and Pathobiochemistry and Spokesperson of the Cells-in-Motion Cluster of Excellence at Universität, Münster, Germany The JDRF-nPOD Scientists Who Seek the Cure for T1D Each month, we highlight one of the nPOD investigators. Thanks to the fundraising of all the Breakthrough T1D chapters, these scientists are able to have access to
- [July 2015 Investigator Spotlight](https://npod.org/news-events/july-2015-investigator-spotlight/) - Investigator Spotlight: Noel Morgan, Director of the Institute of Biomedical and Clinical Science, University of Exeter Medical School
- [2014 Publications (31)](https://npod.org/publications/2014-publications/) - Bogdani M, Korpos E, Simeonovic CJ, Parish CR, Sorokin L, Wight TN. (2014) Extracellular matrix components in the pathogenesis of type 1 diabetes. Current Diabetes Reports. 2014 Dec;14(12):552. doi: 10.1007/s11892-014-0552-7. Donath MY, Hess C, Palmer E. (2015) What is the role of autoimmunity in type 1 diabetes? A clinical perspective. Diabetologia. 2014 Apr;57(4):653-5. doi: 10.1007/s00125-013-3153-0.
- [2009 Publications (3)](https://npod.org/publications/2009-publications/) - In Print Yip, L., Su, L., Sheng, D., Chang, P., Atkinson, M., Czesak, M., Albert P.R., Collier, A., Turley, S.J., Fathman, C.G., & Creusot, R.J. (2009). Deaf1 isoforms control the expression of genes encoding peripheral tissue antigens in the pancreatic lymph nodes during type 1 diabetes. Nature Immunology, 10 (9), 1026 - 1033. Atkinson, M.A.
- [2008 Publications (1)](https://npod.org/publications/2008-publications/) - Spencer, J. & Peakman, M. (2008). Post-mortem analysis of islet pathology in type 1 diabetes illuminates the life and death of the ß cell. Clinical and Experimental Immunology, 155, 125-127.
- [Email Verification](https://npod.org/uncategorized/email-verification/) - [email_verification]
- [Detection of coxsackievirus infection in β-cells in T1D by short fluorescently labeled oligonucleotide probes](https://npod.org/current-npod-projects/coxsackievirus/) - Type 1 diabetes (T1D) results from a complex interplay between genetic polymorphisms, immune system and environment. Viruses, mainly enteroviruses such as members of the Coxsackie B virus (CVB) family, have been suspected since long time to trigger diabetes and epidemiological studies support a causative role of viral infections during diabetes onset. Nonetheless, the presence of
- [Beta cell polarity](https://npod.org/current-npod-projects/beta-cell-polarity/) - Insulin secretion is principally regulated by the beta cell response to glucose. This secretory response is multiphasic with an initial burst of insulin secretion followed by sustained or increasing secretion. This complex timing of secretion is important for body function and is lost in type 2 diabetes. Beta cells normally function, and are normally regulated,
- [nPOD Webinar: Using Pancreas Tissue Slices for in Situ Studies of Human Islet Cell Biology](https://npod.org/news-events/npod-webinar-using-pancreas-tissue-slices-for-in-situ-studies-of-human-islet-cell-biology/) - nPOD Webinar: Using Pancreas Tissue Slices for in Situ Studies of Human Islet Cell Biology nPOD has scheduled a Webinar for Dr. Stephan Speier to discuss the Slice Technique he developed for pancreatic islets and exocrine. From Dr. Speier: "Pancreas tissue slices are 150-200µm thick sections of living pancreatic tissue obtained from animals or humans.
- [Glucagon secretion and islet neuropathy](https://npod.org/current-npod-projects/glucagon-secretion-and-islet-neuropathy/) - To confirm and publish our preliminary observations (Diabetes 55 (supplement 1):64-OR, 2006) suggesting that humans with Type 1 Diabetes Mellitus have a marked and islet-selected loss of their sympathetic nerves, similar to that we have observed in animal models of autoimmune diabetes. If true such a defect may contribute to the impaired glucagon response to
- [Novel recombinant antibodies directed to diabetes-associated autoreactive T cell epitopes](https://npod.org/current-npod-projects/novel-recombinant-antibodies-directed-to-diabetes-associated-autoreactive-t-cell-epitopes/) - The trimolecular complex composed of autoreactive T-cell receptor, MHC class II, and an autoantigenic peptide plays a central role in the activation of pathogenic islet-specific CD4+ T cells in type 1 diabetes (T1D). We isolated and characterized novel antibodies against autoreactive T-cell epitopes associated with T1D. Our antibodies mimic the specificity of the T cell
- [June 2014 Investigator Spotlight](https://npod.org/news-events/7/) - Investigator Spotlight: Sarah Richardson, Ph.D. The JDRF-nPOD Scientists Who Seek the Cure for T1D Each month, we highlight one of the nPOD investigators. Thanks to the fundraising of all the Breakthrough T1D chapters, these scientists are able to have access to nPOD samples for their work. We’ll let them introduce themselves, tell you about
- [April 2014 Investigator Spotlight](https://npod.org/news-events/april-2014-investigator-spotlight/) - April 2014 Investigator Spotlight: Ivan Gerling, Ph.D. The JDRF-nPOD Scientists Who Seek the Cure for T1D Each month, we highlight one of the nPOD investigators. Thanks to the fundraising of all the Breakthrough T1D chapters, these scientists are able to have access to nPOD samples for their work. We’ll let them introduce
- [May 2014 Investigator Spotlight](https://npod.org/news-events/may-2014-investigator-spotlight/) - May 2014 Investigator Spotlight: Joseph Larkin, Ph.D. The JDRF-nPOD Scientists Who Seek the Cure for T1D Each month, we highlight one of the nPOD investigators. Thanks to the fundraising of all the Breakthrough T1D chapters, these scientists are able to have access to nPOD samples for their work. We’ll let them introduce
- [Phenotype, specificity, diversity and clonal spread of B cells in T1DM](https://npod.org/current-npod-projects/phenotype-specificity-diversity-and-clonal-spread-of-b-cells-in-t1dm/) - In this preliminary screening of tissues, we propose to characterize B cells in the pancreas of cadavers that are autoantibody positive. Pancreas of non-autoantibody positive cadavers will be studied as controls. We propose to use immunohistochemistry to analyse cell surface marker expression, together with an analysis of the microantomical distribution of B cells and their relationship to other cells.
- [Histological differences between Japanese and Western type 1 diabetes](https://npod.org/current-npod-projects/histological-differences-between-japanese-and-western-type-1-diabetes/) - (1) To clarify the infiltration of mononuclear cells in exocrine pancreas of Western type 1A diabetes and compare the histological findings to those of Japanese patients (2) To clarify the expressions of the molecules related to innate immunity or viral infection in Western type 1A diabetes and compare the hitological findings to those of Japanese
- [OPO and Recovery Workshop October 22-24, 2014](https://npod.org/news-events/opo-and-recovery-workshop-october-22-24-2014/) - September 15, 2014 is the deadline to register for this event. Recovery partners are invited to attend the 5th annual OPO workshop October 22-24, 2014 at the University of Florida.The Network for Pancreatic Organ Donors with Diabetes (nPOD) relies on research organ referrals from organ procurement organizations to advance the research of over 100 scientific
- [Analysis of Class I MHC expression in islets from patients with type 1 diabetes](https://npod.org/uncategorized/analysis-of-class-i-mhc-expression-in-islets-from-patients-with-type-1-diabetes/) - More information to come…
- [Denver AAb Core](https://npod.org/current-npod-projects/denver-aab-core/) - The Autoantibody Core at the University of Colorado works with the Autoantibody Core at UF for quality control/assurance and tests all nPOD samples for autoantibodies to GAD, IA-2, Insulin, and ZnT8, by RIA.
- [Exeter slides now available in Online Pathology](https://npod.org/news-events/1947/) - nPOD Online Pathology Database has obtained scanned images from slides used in an insulitis workshop held in Exeter, UK, November 2013. A group of premier pathologists and investigators (Mark Atkinson, Alex Butler, Martha Campbell-Thompson, Francesco Dotta, Alan Foulis, Peter In’t Veld, Günter Klöppel, Ed Leiter, Noel Morgan and Sarah Richardson) met to review
- [4th Annual OPO Workshop](https://npod.org/news-events/4th-annual-opo-workshop/) - nPOD is hosting its 4th annual OPO workshop in mid-October in Gainesville. This in-depth workshop provides an overview of the nPOD program for coordinators and research managers from our OPO partners around the country. This year 30 OPO representatives attended and will earn either CTBS or CEPTC continuing education credits as the nPOD staff and
- [Important information about the nPOD review of HIRN‐related requests](https://npod.org/uncategorized/important-information-about-the-npod-review-of-hirn‐related-requests/) - February 15, 2014 TO: All prospective applicants to the NIH (NIDDK) “HIRN” RFAs (RFA‐DK‐13‐013, RFA‐DK‐13‐014, RFA‐ DK‐13‐015, RFA‐DK‐13‐016, RFA‐DK‐13‐017, RFA‐DK‐13‐018) FROM: The nPOD SUBJECT: Important information about the nPOD review of HIRN‐related requests The nPOD will hold a special review of HIRN projects and provide a formal letter in response to your request after such
- [Ljungan virus in T1D](https://npod.org/current-npod-projects/ljungan-virus-in-t1d/) - It was earlier reported the finding that the incidence of type 1 diabetes correlates with small rodent abundance in Sweden. This and other findings initiated an effort to isolate novel infectious agents from small rodents in an attempt to find an etiological agent for type 1 diabetes in humans. As a result of these efforts,
- [nPOD hosts conference, scientific update](https://npod.org/news-events/npod-hosts-conference-scientific-update/) - Leading type 1 diabetes investigators from around the world joined nPOD project partners, along with representatives from Breakthrough T1D and the National Institutes of Health (NIH), for nPOD’s 2nd Annual Conference and Scientific Update on October 19-20, 2008 in Washington, DC. Susan Bonner-Weir,Ph.D., Joslin Diabetes Center and Harvard University, delivered the keynote address entitled, “Of mice and man: what we
- [nPOD Annual Meeting Agenda February 23 - 26, 2014 ](https://npod.org/news-events/npod-annual-meeting-agenda-february-23-26-2014/) - nPOD Annual Meeting Agenda (View PDF) February 23 - 26, 2014 One Ocean Resort Atlantic Beach, FL While previous Annual nPOD Meetings have received much in the way of positive feedback, this year’s meeting has been organized to not only continue in that tradition, but see it in a unique way designed to maximize discussion
- [nPOD 2014 Annual Meeting, February 23-26, 2014, Atlantic Beach, FL](https://npod.org/news-events/npod-2014-annual-meeting-february-23-26-2014-atlantic-beach-fl/) - The 2014 nPOD annual meeting has reached its capacity! We regret that no more registrations can be accepted. Thank you for your interest in this event and support of nPOD. We are happy to announce the Call for Abstract, 2014 JDRF-Helmsley nPOD Annual Scientific Symposium. The meeting will be held February 23-26, 2014 in Atlantic
- [Welcome new nPOD Investigator Coordinator, Dr. Mingder Yang](https://npod.org/news-events/march-2013-newsletter/) - Click here for March 2013 Newsletter! Welcome to Mingder Yang, Ph.D. as the new nPOD Investigator Relations Coordinator! Dr. Yang received a Master's Degree in Bacteriology and a PhD. in Animal Nutrition and Immunology from the University of Wisconsin-Madison. Before joining nPOD, he co-led a start-up biotechnology company, commercializing a patented antibody technology he co-invented. As
- [nPOD hosts 4th annual scientific meeting](https://npod.org/news-events/npod-hosts-4th-annual-scientific-meeting/) - nPOD hosted its 4th Annual Meeting in Miami, Florida on January 15th, 16th, and 17th of this year. There were over 100 participants in this year's meeting where type 1 diabetes science was presented in 8 sessions. We are excited to report that the underlying theme of the meeting was investigator collaboration and our hope
- [nPOD at UF Welcomes Guest Presenter](https://npod.org/news-events/npod-at-uf-welcomes-guest-presenter/) - Who: Huriya Beyan, Ph.D., Blizard Institute, Barts and the London School of Medicine and Dentistry, London, UKWhat: A DNA Methylation Signature Associated with Risk of Childhood-onset Type 1 DiabetesWhen: Wednesday, November 2nd at 9:30 A.M.Where: Biomedical Sciences Building, Room JG-32, University of Florida,Gainesville, Florida Dr. Huriya Beyan is doing her postdoctoral studies with Dr. Richard Leslie at the University of London
- [Congratulations to Dr. Jake Kushner](https://npod.org/news-events/congratulations-to-dr-jake-kushner/) - Congratulations to nPOD investigator, Jake Kushner, M.D., who recently moved to Baylor College of Medicine (BCM)where he has been named a McNair Scholar in diabetes and section head of pediatric diabetes and endocrinology at BCM. Additionally, he has been named service chief of pediatric diabetes and endocrinology at Texas Children's Hospital. Please click here to read more.
- [IIAM Webinars for CEU Credits for OPO Professionals](https://npod.org/news-events/iiam-webinars-for-ceu-credits-for-opo-professionals/) - The nPOD project is now partnering with IIAM to offer CEUs to organ procurement professionals. If you would like to participate in an upcoming Webinar, please contact Gina Dunne-Smith at IIAM for additional information. The learning objectives include: Recognize the difference between type 1 diabetes and type 2 diabetes Understand the Natural History of type 1 diabetes
- [nPOD Welcomes new Partners](https://npod.org/news-events/npod-welcomes-new-partners/) - nPOD is pleased to welcome two new partners to the nPOD Network: The International Institute for the Advancement of Medicine (IIAM) IIAM is one of the largest recovery and placement networks in the world for donated human organs and tissues. IIAM honors the wishes of donors and their families who seek to contribute to medical
- [Investigator Updates](https://npod.org/news-events/investigator-updates/) - nPOD Viral Workgroup After months of hard work and an overwhelming spirit of collaboration, the nPOD-Viral working group, which focuses on the role of viruses in type 1 diabetes, has defined key questions that the group will address together. The work has been organized in major tasks, and experimental plans have been developed and submitted
- [nPOD now has 19 cases with insulitis in our biorepository](https://npod.org/news-events/npod-now-has-19-cases-with-insulitis-in-our-biorepository/) - nPOD now has 19 cases with insulitis in our biorepository, including a case recovered at onset of diabetes in the spring 2013. We want to thank our organ procurement partners and donor families for these precious gifts to research. While much can be learned from tissue collected at all stages of the disease; many believe
- [2011 Publications (6)](https://npod.org/publications/2011-publications/) - In Print Coppieters KT, Roep BO, von Herrath MG. (2011). Beta cells under attack: toward a better understanding of type 1 diabetes immunopathology. Semin Immunopathol., 33 (1):1-7. Greiner, D. L., Brehm, M. A., Hosur, V., Harlan, D. M., Powers, A. C., & Shultz L. D. (2011). Humanized mice for the study of type 1 and
- [nPOD Presents at 2013 ADA Annual Meeting, Chicago, IL](https://npod.org/news-events/npod-presents-at-2013-ada-annual-meeting-chicago-il/) - Dr. Alberto Pugliese gave a great talk at this year ADA annual meeting, titled New Insights to Human Type 1 Diabetes from nPOD. If you missed his talk in Chicago and would like to review the presentation, please click and downland the PDF file. New Insights to Human Type 1 Diabetes from nPOD A tissue
- [nPOD Welcomes New Scientific Investigators](https://npod.org/news-events/npod-welcomes-new-scientific-investigators/) - InvestigatorAffiliationProject Area Jerry Nepom Benaroya Research Institute at Virginia Mason Immunology Al Powers Vanderbilt University Islet and Pancreas Pathology Eric Triplett and Martha Campbell-Thompson University of Florida Disease Etiology Ake Sjoholm Karolinska Institutet Disease Etiology
- [nPOD to attend ADA, AOPO and NATCO Conferences](https://npod.org/uncategorized/npod-to-attend-ada-aopo-and-natco-conferences/) - nPOD staff will be on site at a number of scientific and professional conferences this summer. Please be sure to visit our booth if you have plans to be at any of the following events: American Diabetes Association (ADA), 69th Scientific Sessions, June 2009, New Orleans Association of Organ Procurement Organizations (AOPO), Annual Meeting, June 2009, St. Louis,
- [Investigator Spotlight: Humanizing the Mouse for nPOD Tissue Engraftment](https://npod.org/news-events/investigator-spotlight-humanizing-the-mouse-for-npod-tissue-engraftment/) - by Nicole N. Parker Dr. Dale Greiner of the University of Massachusetts School of Medicine has been collaborating with Dr. Len Shultz, of the Jackson Laboratory, for over 25 years. He and Dr. Shultz have developed a mouse model for human type I diabetes (T1D). This “humanized mouse” model is a severely immunodeficient mouse strain
- [nPOD Wins Poster of Distinction at NATCO Conference](https://npod.org/news-events/npod-wins-poster-of-distinction-at-natco-conference/) - Congratulations to Patrick Rowe, PhD, whose poster presentation: "Network for Pancreatic Organ Donors with Type 1 Diabetes (nPOD): Progress and Donor Demographics" won Top Abstract/Case Study presented in a Poster Format at the NATCO 34th Annual Meeting in August, 2009. NATCO is committed to the advancement of organ and tissue donation and transplantation. Its membership is comprised of
- [nPOD Welcomes New Investigators - Fall 2009](https://npod.org/news-events/npod-welcomes-new-investigators-fall-2009/) - nPOD is pleased to welcome the following new investigators to the project: Akihisa Imagawa, MD, PhDOsaka UniversityHistological Differences between Japanese and Western Type 1 Diabetes Sara Michie, PhDStanford UniversityTissue-selective Chemokines and Adhesion Molecules in Type 1 Diabetes Jerry Nadler, MDEastern Virginia Medical School12/15 Lipoxygenase Expression in Type 1 Diabetes Suparna Sarkar, PhD and Dirk Homann, PhDUniversity
- [Research Spotlight: A New Angle on the Progression of Type 1 Diabetes](https://npod.org/news-events/research-spotlight-a-new-angle-on-the-progression-of-type-1-diabetes/) - Jo Spencer, Ph.D., King's College London School of Medicine Dr. Jo Spencer has published extensively on immune cells that reside in the gastrointestinal system, or gut. Because this organ shares many similarities with the pancreas, Dr. Spencer has brought her expertise into the field of type 1 diabetes (T1D). Dr. Spencer’s research transitioned from the
- [Breakthrough T1D Announces nPOD RFA for Investigators](https://npod.org/news-events/jdrf-announces-npod-rfa-for-investigators/) - We are pleased to announce that Breakthrough T1D has created a new funding opportunity. Below please find the nPOD RFA: Link to the nPOD RFAPlease also note the following deadlines: Letter of Intent deadline: December 28, 2009Application deadline: February 8, 2010Please visit the Requests for Application section of the Breakthrough T1D website for more information. And please
- [Several nPOD Investigators to be Published in Diabetologia](https://npod.org/news-events/several-npod-investigators-to-be-published-in-diabetologia/) - It is our privilege to announce that several of our investigators (Roberto Gianani, MD, Martha Campbell-Thompson, DVM, PhD, Suparna A. Sarkar, MBBS, PhD, Alberto Pugliese, MD, Sally Kent, PhD, Susan Bonner-Weir, Ph D, Mark A. Atkinson, PhD, and Matthias von Herrath, MD) co-authored a paper entitled: "Dimorphic Histopathology of Long Standing Childhood-Onset Diabetes," which is to be
- [Investigator Spotlight: Drug Therapy as a Treatment for Type 1 Diabetes](https://npod.org/news-events/investigator-spotlight-drug-therapy-as-a-treatment-for-type-1-diabetes/) - Matthias von Herrath, M.D., La Jolla Institute for Allergy and Immunology, University of California, San Diego Dr. Matthias von Herrath, Director of the Center for Type 1 Diabetes Research at the La Jolla Institute for Allergy and Immunology (LIAI) at UCSD, has dedicated his career to the study of type 1 diabetes (T1D). Together with his
- [Welcome to New nPOD Investigators](https://npod.org/news-events/welcome-to-new-npod-investigators-5/) - Project Name: Generation of tools to distinguish human pancreatic cell populationsPI: Douglas Melton, Ph.D., Harvard University Project Name: E-cadherin mediates developmental effects on the proliferation and the function of ß-cells in the islet of LangerhansPI: Rudy Leibel, M.D.Columbia UniversityCo-PI: Nao Wakae, M. Phil. Columbia University Project Name: Microangiopathy in diabetic bone marrowPI: Paolo Madeddu, M. D.,University of Bristol, UKCo-PI: Costanza Emanueli, Ph.D.,University of Bristol, UK Project Name: Peri-capsular basement
- [Welcome to New nPOD Investigators](https://npod.org/news-events/welcome-to-new-npod-investigators-4/) - nPOD is pleased to welcome 10 new investigators. nPOD now supports nearly 50 investigators all over the world that are invested in type 1 diabetes research. These investigators will use nPOD tissues to better understand T1D and what causes it in the hopes of finding a cure. Below is a list of recently approved projects:
- [Here We Grow Again](https://npod.org/news-events/here-we-grow-again/) - Congratulations to Alberto Pugliese, M.D., who has accepted the position of nPOD associate executive director. Welcome Dr. Pugliese! Thank you to Sally Kent, Ph.D., and the Publications and Presentations Committee for their efforts to draft a governing document for nPOD-based manuscripts and presentations. Please visit the Publications page of our website for more information. Based on the nPOD model for collaborative
- [Welcome to New nPOD Investigators](https://npod.org/news-events/welcome-to-new-npod-investigators-3/) - Congratulations to the 16 investigators who received Breakthrough T1D funding for nPOD based studies. These awards began July 1, 2010 and funding for each project ranges between $50,000 and $210,000. nPOD now supports over 60 international investigators engaged in efforts to better understand the causes of type 1 diabetes and find ways to prevent and
- [Breakthrough T1D Funded Investigators with New nPOD Projects:](https://npod.org/news-events/jdrf-funded-investigators-with-new-npod-projects/) - Project Name: Identification of extrathymic Aire-expressing cells in human lymph nodesPI: Mark Stuart Anderson, M.D., Ph.D., University of California, San Francisco Project Name: Tracing effector and regulatory T-cell populations in type 1 diabetesPI: Dolores Jaraquemada, Ph.D., Universitat Autonoma de Barcelona Project Name: Risk of autoimmune diseases and human self-antigen expressionPI: Richard David Leslie, M.D, FRCP, University of London Project Name: Sequencing the islet transcriptomePI: Ivan
- [Changes in the New Year at nPOD](https://npod.org/news-events/changes-in-the-new-year-at-npod/) - Jayne Moraski, MS, Assistant Director, OPO and Lab RelationsJayne Moraski, MS, is the Assistant Director of nPOD and will work primarily to coordinate relationships with the nPOD screening laboratories and organ procurement organizations (OPO). Please contact Jayne if you are an OPO interested in working with nPOD. Jayne also coordinates nPOD educational webinars, manages nPOD's
- [Our deepest sympathies to the loved ones of Mark D. Pescovitz, M.D.](https://npod.org/news-events/our-deepest-sympathies-to-the-loved-ones-of-mark-d-pescovitz-m-d/) - Dr. Mark Pescovitz, esteemed nPOD investigator and professor at the Indiana University School of Medicine was killed in an automobile accident on December 12, 2010. Dr. Pescovitz was an accomplished physician with a passion for type 1 diabetes research, as well as a loving husband and father of three children. We extend our condolences to
- [Welcome to New nPOD Investigators](https://npod.org/news-events/welcome-to-new-npod-investigators/) - Project Name: Pancreatic duct glands in type 1 diabetesPI: Peter Bulter, M.D., University of California, Los AngelesCo-PI: Alexandra Butler, M.D.Co-PI: Mark Atkinson, Ph.D., University of Florida Project Name: TCR signal transduction in diabetogenic T cellsPI: Raphael Clynes, M.D., Ph.D., Columbia University Project Name: Bone marrow progenitor cell (BMPCs) dysfunction in diabetes is mediated by reduced bioavailability of NOPI: Maria Grant, M.D., University of
- [2012 Annual Meeting Call for Abstracts](https://npod.org/news-events/2012-annual-meeting-call-for-abstracts/) - We are pleased to announce the Call for Abstracts for the 2012 nPOD Annual Meeting. The meeting will be held January 15-17 in sunny Miami, Florida. For more information on the Call for Abstracts and to access the abstract template, please click here
- [Meeting/Conference Updates](https://npod.org/news-events/meetingconference-updates/) - nPOD's Organ Procurement Organization (OPO) Workshop We hosted our 2nd annual OPO workshop in mid-October in Gainesville. This in-depth workshop provides an overview of the nPOD program for coordinators and research managers from our OPO partners around the country. This year 20 OPO representatives attended and earned either CTBS or CEPTC continuing education credits as the
- [Exciting NEW Opportunities](https://npod.org/news-events/exciting-new-opportunities/) - nPOD invited to present at the American College of Emergency Physicians (ACEP) meeting nPOD is always looking for innovative ways to collaborate with health care providers in order to identify and provide rare and precious type 1 diabetic specimens to our investigators. Dr. Alberto Pugliese, nPOD Co-Executive Director, was invited to speak at their annual
- [Farewell to Amy Wright](https://npod.org/news-events/farewell-to-amy-wright/) - We wish Amy the best of luck as she leaves the nPOD family to get married and begin a family of her own. Congratulations to Amy and Dylan, we will miss you! Please contact Carmen Retrum with any investigator related questions or concerns you might have, including those for the Viral Workgroup, as she will
- [Breakthrough T1D Announces New Funding Opportunity](https://npod.org/news-events/jdrf-announces-new-funding-opportunity/) - We are pleased to announce that Breakthrough T1D is soliciting Expressions of Interest (EOI) for the study of modifications of beta cell antigens in the immunopathogenesis and treatment of type 1 diabetes. Below please find more information on this funding opportunity: Link to download PDF Please note the following deadlines:Expression of Interest: September 9, 2011 Application
- [Congratulations OPPC!](https://npod.org/news-events/congratulations-oppc/) - OPPC earns high score We are excited to report that the nPOD Organ Processing and Pathology Core (OPPC) lab recently scored a 95.9% on the International Society for Biological and Environmental Repositories (ISBER) assessment! ISBER is the only international forum that addresses the technical, legal, ethical, and managerial issues relevant to repositories of biological and environmental

## Pages

- [Home](https://npod.org/) - JDRF is committed to finding a method for preventing or permanently reversing type 1 diabetes; an effort that would undoubtedly benefit from an improved understanding of how type 1 diabetes develops.
- [Cores](https://npod.org/about/cores/) - Cores The operations for recovering, sample processing and distributing and data management of nPOD donor gifts are done through four different cores: Administrative Core Autoantibody Core Data Management Core Organ Processing and Pathology Core (OPPC) Large Scale Electron Microscopy (EM) Core Administrative Core The Administrative Core, located at the University of Florida, coordinates relationships with
- [Organ Donor Registration](https://npod.org/about/organ-donor-registration/) - Organ Donor Registration At nPOD we work with the fifty-six federally designated Organ Procurement Organizations (OPOs) in the United States to receive pancreata from suitable organ donors to help our research into Type 1 diabetes. These pancreata are recovered in the same procedure and by the same team as organs that are being recovered for life-saving
- [2023 Annual Meeting Attendees](https://npod.org/attendees/) - Download the Attendees List Here
- [UF T1D Program Donor Offer](https://npod.org/uf-t1d-program-donor-offer/)
- [Recap](https://npod.org/recap/) - A SPECIAL THANK YOU GOES OUT TO OUR 2023 EVENT SPONSORS & SUPPORTERS! Funding for this grant is made possible through collaboration between Breakthrough T1D International and The Leona M. and Harry B. Helmsley Charitable Trust.
- [Registration](https://npod.org/annual-meeting-registration/)
- [nPOD Live Webinar Registration: Erik Finger, MD, PhD](https://npod.org/npod-live-webinar-registration-erik-finger-md-phd/)
- [How We Work](https://npod.org/about/how-we-work/) - How We Work The goals of the nPOD project are to recover relevant tissue from our donor groups, share these gifts with approved scientific investigators who seek to prevent, reverse and ultimately cure the disease and foster collaboration amongst these investigators. In order to achieve these goals, nPOD is comprised of various committees and boards
- [Contact Us](https://npod.org/contact-us/)
- [Publications](https://npod.org/publications/npod-publications/)
- [Investigator-Related Frequently Asked Questions](https://npod.org/investigator-related-frequently-asked-questions/)
- [Apply to Join nPOD](https://npod.org/for-investigators/apply-to-join-npod/) - Apply to Join nPOD For New nPOD Applicants First-time applicants in academia should use the nPOD New Project and Addendum Application Form and applicants in industry should use the nPOD New Project and Addendum Application Form - Industry. Both will also need to submit a completed nPOD Material Transfer Agreement (nPOD MTA) to apply to become
- [Donor Groups](https://npod.org/for-investigators/donor-groups/) - nPOD Donor Groups For any questions, please contact nPOD.
- [Donor Groups DRAFT](https://npod.org/for-investigators/donor-groups-3/) - nPOD Donor Groups For any questions, please contact nPOD.
- [Who We Are](https://npod.org/about/who-we-are/) - Mission Type 1 diabetes, also known as 'juvenile diabetes', results from a self-destructive immune response against the insulin producing pancreatic beta cells. As a result of this so-called 'autoimmune' disease, patients with type 1 diabetes develop a life long dependence on insulin replacement therapy. Unfortunately, this form of treatment is often insufficient for preventing a
- [Eisenbarth nPOD Award Recipients](https://npod.org/eisenbarth-npod-award-recipients/) - List of 56 Grants to nPOD Investigators from the Helmsley Charitable Trust George S. Eisenbarth nPOD Award for Team Science (2015-2021, as of October 13, 2021, from Grant awards 2018-PG-T1D-060 and 2015-PG-T1D-052) Awards made in support of pilot studies: Butler, Alexandra. UCLA, Los Angeles, CA, USA. Growth and Development of the Endocrine Pancreas in Humans: Extrapolating
- [Organ Recovery Partners](https://npod.org/for-partners/organ-recovery-partners/) - Organ Recovery Partners Our organ recovery partners help us by identifying donors that meet nPOD's donor criteria, recovering and delivering designated organs and tissues from those donors and obtaining consent for research from donor families. Each donation to research has the potential to save thousands of lives. Our organ procurement organization (OPO) partners provide the
- [Publication Policies](https://npod.org/publications/policies/) - Publication Policies If you are currently an nPOD investigator, we ask that you become and remain familiar with the nPOD Publication and Presentations Policies. This policy document includes acknowledging statements, governs the use of nPOD images in publications, explains how to identify nPOD samples in your work, and provides other guidelines related to presentations and publications.
- [Helmsley Charitable Trust Grant](https://npod.org/publications/helmsley-charitable-trust-grant/) - Helmsley Charitable Trust George S. Eisenbarth nPOD Award for Team Science The Helmsley Charitable Trust George S. Eisenbarth nPOD Award for Team Science was instituted in 2014. Funds from this award are dedicated to support the research activities of nPOD investigators to catalyze progress in critical areas of T1D. It is critical to provide pilot
- [nPOD KQ group: Role of the exocrine pancreas in T1D development](https://npod.org/publications/npod-working-groups/npod-kq-group-role-of-the-exocrine-pancreas-in-t1d-development/)
- [nPOD KQ group: Understanding the heterogeneity of T1D](https://npod.org/publications/npod-working-groups/npod-kq-group-understanding-the-heterogeneity-of-t1d/)
- [Current nPOD Projects](https://npod.org/publications/current-npod-projects/)
- [Request nPOD Genetic Data](https://npod.org/for-investigators/request-npod-genetic-data/) - Request Samples via DataShare How to request nPOD samples via DataShare Order nPOD samples through DataShare. Please reference the nPOD DataShare User Guide for instructions on how to order samples and view donor data. If you are not familiar with the DataShare sample ordering system, please view the following webinar videos: ♦ Streaming Video, November
- [Standard Operating Procedures](https://npod.org/for-investigators/standard-operating-procedures/) - Standard Operating Procedures The nPOD Standard Operating Procedures are available to all nPOD investigators and partners in PDF format. These procedures are approved by the nPOD administrative core. A brief description of each procedure or protocol is provided for your convenience below. If you have any questions about any of the information included in these
- [nPOD 2025 OPO Workshop Registration](https://npod.org/npod-2025-opo-workshop-registration/)
- [nPOD Recovery Checklist - Kidney](https://npod.org/npod-recovery-checklist-kidney/)
- [nPOD Recovery Checklist - Heart with Aortic Arch](https://npod.org/npod-recovery-checklist-heart-with-aortic-arch/)
- [nPOD Working Groups](https://npod.org/publications/npod-working-groups/) - nPOD Working Groups The nPOD working groups investigate specific points of interest in diabetes research. For concept proposals, please submit the sections of the nPOD Project Application Form that corresponds to working groups. nPOD Viral nPOD-V represents a self-assembled collaborative effort with the goal of investigating the role of viruses in T1D through the
- [nPOD 2025 Annual Meeting Survey](https://npod.org/2025-annual-meeting-survey/)
- [nPOD 2025 Program](https://npod.org/program/) - Click here for PDF view.
- [2025 Abstracts](https://npod.org/abstracts/) - Abstracts - Coming Soon
- [ANNOUNCEMENTS](https://npod.org/announcements/) - test
- [Speakers](https://npod.org/speakers/)
- [Sponsors](https://npod.org/sponsors/) - A SPECIAL THANK YOU GOES OUT TO OUR 2023 EVENT SPONSORS & SUPPORTERS! Funding for this grant is made possible through collaboration between Breakthrough T1D International and The Leona M. and Harry B. Helmsley Charitable Trust.
- [Type 1 Diabetes Research Community](https://npod.org/about/t1d-research-community/)
- [HOTEL INFORMATION](https://npod.org/hotel-information/)
- [nPOD 2025 Annual Scientific Meeting Welcome](https://npod.org/annual-meeting/)
- [Online Pathology Information](https://npod.org/for-investigators/online-pathology-information/) - Online Pathology Information. Welcome to nPOD online pathology All scientific investigators have access to our password protected online pathology site. On this site, you will be able to view whole slide scanned images of H&E and IHC stained tissue sections for each nPOD donor. These images are created using an Aperio ScanScope CS system. To
- [For Investigators](https://npod.org/for-investigators/) - For Investigators Apply to Join nPOD Learn how to become an approved nPOD investigator and download an application. Current nPOD investigators can find forms to order nPOD samples. Helmsley Charitable Trust Grant George S. Eisenbarth nPOD Award for Team Science Donor Groups Review nPODs donor groups. Online Pathology Information All scientific
- [2024 Speakers](https://npod.org/speakers-2/) - Meeting Speakers - Coming Soon
- [nPOD 2024 OPO Workshop Registration](https://npod.org/npod-2024-opo-workshop-registration/)
- [nPOD KQ Group: Prohormone processing defect in T1D pancreas](https://npod.org/publications/npod-working-groups/npod-kq-group-prohormone-processing-defect-in-t1d-pancreas/)
- [nPOD KQ Group: Is there a difference between type 1 diabetes in children and adults?](https://npod.org/publications/npod-working-groups/npod-kq-group-is-there-a-difference-between-type-1-diabetes-in-children-and-adults/)
- [Data Portal](https://npod.org/for-investigators/data-portal/) - Data Portal Introduction The nPOD Data Portal site functions as a hub for accessing the latest information on cases collected by the biorepository, sample inventory currently available for study, and datasets provided by nPOD investigators and collaborators. The nPOD DataShare legacy site will be available for sample request placement and limited data access. The most
- [2022 Poster Abstracts](https://npod.org/agenda-2/) - Download Poster Abstracts
- [nPOD Webinars](https://npod.org/news-events/npod-webinars/)
- [nPOD Case RRIDs](https://npod.org/publications/rrid/) - The Resource Identification Portal The Resource Identification Portal promotes research resource identification, discovery, and reuse. The Resource Identification Portal is a central location where researchers can obtain and explore Research Resource Identifiers (RRIDs). RRIDs are unique identifiers for referencing a source for research in the manuscripts, and are based on public information that is not
- [Submission Form](https://npod.org/publications/submission-form/) - Submission Form nPOD encourages scientific publications and talks that utilize nPOD samples. Every time you submit an abstract or publication, please use this form. Please see the Investigator Publications Policy for more information on image use, nPOD case nomenclature, and more. Please complete the form below.
- [Request Samples via DataShare](https://npod.org/for-investigators/request-npod-samples/) - Request Samples via DataShare How to request nPOD samples via DataShare Order nPOD samples through DataShare. Please reference the nPOD DataShare User Guide for instructions on how to order samples and view donor data. If you are not familiar with the DataShare sample ordering system, please view the following webinar videos: ♦ Streaming Video, November
- [nPOD KQ group: Beta- and/or islet specific targets for a variety of studies](https://npod.org/publications/npod-working-groups/npod-kq-group-beta-and-or-islet-specific-targets-for-a-variety-of-studies/)
- [OPO Recovery Instructions](https://npod.org/for-investigators/opo-recovery/) - OPO Recovery Instructions For all OPOs that refer directly to nPOD, please call 1-866-731-6585 For those that refer through IIAM, please call 1-800-486-4426 For those that refer through NDRI, please call 1-800-222-6374 For those that refer through Novabiosis, please email researchoffer@novabiosis.com Acceptance Criteria and Recovery Instructions Acceptance Criteria Here at nPOD we are very grateful for
- [nPOD Partners](https://npod.org/for-partners/npod-partners/) - nPOD Partner Organizations nPOD's success is dependent on its many partner organizations. These organizations assist nPOD with organ recovery and delivery and ensure that research consent is obtained from donor families. For more information on our partner organizations, please select a link below: Organ Recovery Partners Screening Lab Partners
- [Screening Lab Partners](https://npod.org/for-partners/screening-lab-partners/) - nPOD Screening Lab Partners Our screening laboratory partners are nPOD's "detectives," identifying donors that are in early stages of pre-type 1 diabetes (have type 1 diabetes autoantibodies) but have no clinical diagnosis of the disease. Our laboratory partners screen for multiple OPOs and are recognized as national leaders in serology testing. Our laboratory partners test
- [nPOD Omics Working Group](https://npod.org/publications/npod-working-groups/npod-omics/) - nPOD Omics Working Group As the nPOD sample repository grows and valuable samples are depleted in the quest to understand the pathogenesis and pathology of Type 1 diabetes, there is a growing need to establish standardized ‘omics data for each precious sample. To realize this goal, the nPOD “omics” group was created, with the following
- [nPOD KQ group: Translation studies to advance T1D therapies](https://npod.org/publications/npod-working-groups/npod-kq-group-translation-studies-to-advance-t1d-therapies/)
- [nPOD Recovery Checklist - Panc Pediatric](https://npod.org/npod-recovery-checklist-panc-pediatric/)
- [nPOD Recovery Checklist - Panc Adult](https://npod.org/npod-recovery-checklist-panc-adult/)
- [nPOD West Slices/Islets Distribution](https://npod.org/npod-west-slices-islets-distribution/)
- [nPOD 2024 Annual Meeting Survey](https://npod.org/npod-2024-annual-meeting-survey/)
- [nPOD Transplant Working Group](https://npod.org/publications/npod-working-groups/npod-transplant/) - nPOD Transplant Working Group What is nPOD-T? The nPOD-Transplant (nPOD-T) Working Group is a JDRF-funded study, based at the University of Miami in Miami, Florida, aimed at scientifically researching human pancreatic tissue from organ donors who have had a pancreas transplant. By studying both transplant and native tissue from these donors, nPOD-T will fill a
- [nPOD ECM Working Group](https://npod.org/publications/npod-working-groups/npod-ecm/) - nPOD ECM Working Group The nPOD extracellular matrix working group (nPOD-ECM) is comprised of teams of researchers working together with the goal of investigating the role of extracellular matrix (ECM) in type 1 diabetes through the study of nPOD samples. Our three research teams include: Marika Bogdani, Benaroya Research Institute Charmaine Simeonovic and Christopher Parish,
- [nPOD Autoimmunity Working Group](https://npod.org/publications/npod-working-groups/npod-autoimmunity/) - nPOD Autoimmunity Working Group The Autoimmunity Group operates under the premise that the resident immune cells at the site of autoimmune attack within the pancreas are key to understanding the fundamental pathways of disease development. The major goal of this working group is to elucidate the mechanisms by which autoreactive T cells and B cells,
- [nPOD Viral Working Group](https://npod.org/publications/npod-working-groups/npod-viral-work-group/) - nPOD Viral Working Group nPOD has assembled a “cloud” of investigators with diverse expertise, who are interested in collaborative studies, and is ready to take the nPOD research model to a higher level: the nPOD working groups. These groups are intended to collectively tackle key questions in diabetes research. The nPOD viral working group (nPOD-V)
- [Screening Lab Protocols](https://npod.org/for-investigators/screening-lab-protocols/) - Screening Lab Protocols The nPOD Standard Operating Procedures are available to all nPOD investigators and partners in PDF format. These procedures are approved by the nPOD executive committee. A brief description of each procedure or protocol is provided for your convenience below. If you have any questions about any of the information included in these
- [Search](https://npod.org/search/)
- [Privacy Policy](https://npod.org/privacy-policy/)
- [Recovery Checklist RP2 - Panc (Test)](https://npod.org/recovery-checklist-rp2-panc-test/)
- [npod.org/annual-meeting](https://npod.org/npod-org-annual-meeting/)
- [CORE DRC: nPOD Case Referral](https://npod.org/core-drc-case-referral/)
- [Recovery Checklist RP 16 - Tier 3 Pancreas](https://npod.org/rp16/)
- [Publications](https://npod.org/publications/) - Projects & Publications Current nPOD Projects View a list of nPOD investigators by project area. Publications View current and archived publications that are in print, abstracts and oral presentations. nPOD Case RRIDs View instructions on how to use the Resource Identification Portal and how to add RRIDs to manuscripts. Publication
- [nPOD 2023 Annual Meeting Survey](https://npod.org/survey/)
- [14th Annual Scientific Meeting Attendees List](https://npod.org/attendees-2014/) - In-Person Participants - Download PDF Virtual Participants - Download PDF View speakers and oral presenters here
- [Abstracts](https://npod.org/abstracts4/) - Poster abstracts will be announced soon!
- [Recovery Checklist RP 8 - Whole Intestine, Lung](https://npod.org/rp8/)
- [Recovery Checklist RP7 - Lung](https://npod.org/rp7/)
- [Recovery Checklist RP6 - Panc, Lung, Intestine](https://npod.org/rp6/)
- [Recovery Checklist RP5 - Intestine](https://npod.org/rp5/)
- [Recovery Checklist RP4 - Kidney](https://npod.org/rp4/)
- [Recovery Checklist RP 3 - Panc](https://npod.org/rp3/)
- [Recovery Checklist RP 9 - Panc, Intestine](https://npod.org/rp9/)
- [Recovery Checklist RP15 - Heart with Aortic Arch](https://npod.org/rp15/)
- [Recovery Checklist RP14 - Panc](https://npod.org/rp14/)
- [Recovery Checklist RP 12 - Panc Long Term DM Type 1](https://npod.org/rp12/)
- [Committees](https://npod.org/committees/) - Committees In order to foster collaboration amongst nPOD investigators, the following committees were formed and are investigator-run: Publications and Presentations Committee (PPC) Tissue Prioritization Committee (TPC) Publications and Presentations Committee (PPC) The Publications and Presentations Committee (PPC) specifies policies regarding acknowledgement to the use of nPOD materials and images in all forms of publications and
- [Recovery Checklist](https://npod.org/recovery-checklist/)
- [Frequently Asked Questions](https://npod.org/frequently-asked-questions/) - If you have any additional questions please contact nPOD, at npod@pathology.ufl.edu.
- [Virtual Posters](https://npod.org/virtual-posters/) - The Virtual Poster Presentation will be Monday, January 31st from 5:00 to 6:00 PM EST. Link: https://tinyurl.com/2bdvcvv9 Meeting ID: 824 5897 3710 Password: 941422 If all the videos begin playing at the same time, you may need to disable autoplay on your browser.
- [nPOD 2022 Annual Meeting: Agenda](https://npod.org/agenda2/) - Tentative Meeting Agenda This meeting agenda is still being finalized. A finalized agenda will be coming soon! *All times are in Eastern Standard Time (EST).
- [For Partners](https://npod.org/for-partners/) - For Partners OPO Recovery Instructions Information for OPOs about how to recover, refer and ship donor organs/tissues to nPOD. nPOD Partners Meet the nPOD partners that help make this project possible. Standard Operating Procedures Review nPOD's standard operating procedures. Become an nPOD Partner Learn about what it means to work
- [2022 Annual Meeting Abstract Submission](https://npod.org/2022-annual-meeting-abstract-submission/) - nPOD Investigators are encouraged to submit abstracts for research conducted using nPOD resources. The nPOD 14th Annual Scientific Meeting will be held on January 30 - February 2, 2022 at the One Ocean Resort & Spa at 1 Ocean Blvd, Atlantic Beach, FL 32233. The deadline to submit abstracts will be Sunday, October 31, 2021 at
- [News & Events](https://npod.org/news-events/) - News & Events News & Events Look at a list of all past nPOD news and events. Newsletter Sign Up Form A quarterly e-newsletter for nPOD investigators, partners, and friends of the project. nPOD Webinars View past and upcoming webinars hosted by nPOD Investigators.
- [Recovery Protocols Form](https://npod.org/recovery-protocols-form/)
- [Investigator Spotlight: Sally Kent, Ph.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-sally-kent-ph-d/) - Investigator Spotlight: March 2015 Sally Kent, PhD Dr. Kent is an assistant professor at the University of Massachusetts Medical School. Dr. Kent joined nPOD in 2007. Tell us about your education and background – where are you from, where did you go to school? My name is Sally Kent and I’m from
- [Feedback Survey](https://npod.org/feedback-survey/)
- [2021 Annual Meeting Abstract Submission](https://npod.org/2021-annual-meeting-abstract-submission/) - nPOD Investigators are encouraged to submit abstracts for research conducted using nPOD resources. The nPOD 13th Annual Scientific Meeting will be a virtual event held on February 22-24, 2021. This year, there will be two abstract submission deadlines - one deadline to be considered for an oral presentation, and another deadline for poster presentations. Oral
- [nPOD 2021 Annual Meeting RSVP](https://npod.org/npod-2021-annual-meeting-rsvp/)
- [Donor Groups Test](https://npod.org/donor-groups-test/) - nPOD Donor Groups Donor Type Count Age Range Control (No diabetes, Autoantibody Negative) 214 0.0 to 75.0 Autoantibody Positive (No Type 1 Diabetes) 38 0.17 to 69.2 Type 1 diabetes 167 4.4 to 93.0 Type 1 Diabetes 142 4.4 to 71.2 Type 1 Diabetes Medalist 12 59.0 to 93.0
- [About](https://npod.org/about/) - About Mission Learn more about the nPOD mission. How We Work Explore the administrative structure of nPOD and find contact information for nPOD directors and staff. Type 1 Diabetes Research Community Learn more about the research community nPOD supports. Organ Donor Registration Register to donate to nPOD and help researchers find a cure for diabetes.
- [ADMIN Case Criteria Tracking](https://npod.org/admin-case-criteria-tracking/) - Case Rotation (nPOD - HPAP) Overlapping Criteria Case Table Next Case Program Updated Initials Next Aab+ case nPOD 4/1/2019 KR Next T1D Case HPAP 1/2/2019 KR IIAM Current Criteria: Type 1 diabetes :Pancreas for donors 40 years or less in age with a type 1 disease duration of 10 years or less. Pancreas from any donor
- [Survey: Final Day](https://npod.org/agenda-2023/survey-final-day/)
- [Survey: Cutting Edge Seminar I](https://npod.org/agenda-2023/survey-cutting-edge-seminar-i/)
- [Calendar](https://npod.org/calendar/)
- [Test Acceptance Criteria](https://npod.org/test-acceptance-criteria/)
- [Poll](https://npod.org/poll/)
- [Investigator Spotlight: Teresa Rodriguez-Calvo, DVM, Ph.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-teresa-rodriquez-calvo-dvm-ph-d/) - Investigator Spotlight: December 2015 Teresa Rodriguez-Calvo, DVM, PhD Dr. Rodriguez-Calvo is an instructor, working with Dr. Matthias von Herrath at La Jolla Institute. Tell us about your education and background – where are you from, where did you go to school? I was born in Madrid, Spain. I knew very early in life that I
- [Investigator Spotlight: Alexandra Butler, MBBS](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-alexandra-butler-mbbs/) - Investigator Spotlight: April 2016 Alexandra Butler, MBBS Dr. Butler is an adjunct professor of medicine and endocrinology at City of Hope National Medical Center in Duarte, California. Dr. Butler joined nPOD in 2015. Tell us about your education and background – where are you from, where did you go to school? My childhood
- [Password Request Form](https://npod.org/for-investigators/password-request-form/) - For Investigators. Password Request Form Please complete the form below.
- [nPOD Donor Offer](https://npod.org/npod-donor-offer/)
- [HPPAP Donor Offer](https://npod.org/hppap-donor-offer/)
- [Investigator Spotlight: Georgia Fousteri, PhD](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-georgia-fousteri-phd/) - Investigator Spotlight: August 2016 Georgia Fousteri, PhD Dr. Fousteri is a group leader at the San Raffaele Diabetes Institute in Milan, Italy. Dr. Fousteri joined nPOD in 2014. Tell us about your education and background – where are you from, where did you go to school? I was born in Piraeus, Greece, but raised in
- [nPOD Investigator Spotlights](https://npod.org/news-events/npod-investigator-spotlights/) - nPOD Investigator Spotlights 2016 Investigator Spotlights August 2016: Georgia Fousteri, PhD May 2016: Lars Krogvold, MD April 2016: Alexandra Butler, MBBS March 2016: Richard Oram, MD, PhD January 2016: Agata Jurczyk, PhD 2015 Investigator Spotlights December 2015: Teresa Rodriguez-Calvo, DVM, PhD November 2015: David Leslie, MD October 2015: Antonio Toniolo, MD September 2015: Abdu Hamad, PhD
- [Investigator Spotlight: Agata Jurczyk, Ph.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-agata-jurczyk-ph-d/) - Investigator Spotlight: January 2016 Agata Jurczyk, Ph.D. Dr. Jurczyk is an instructor at the University of Massachusetts. She works with nPOD Investigator, Dr. Dale Greiner. Tell us about your education and background – where are you from, where did you go to school? I grew up on a small farm in Poland and immigrated to
- [Investigator Spotlight: Lars Krogvold, MD](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-lars-krogvold-md/) - Investigator Spotlight: May 2016 Lars Krogvold, MD Dr. Krogvold is a researcher at Oslo University Hospital in Norway. He works with nPOD Investigator, Dr. Knut Dahl- Jorgensen. Tell us about your education and background – where are you from, where did you go to school? I am from Gjerdrum, a small and peaceful
- [Investigator Spotlight: Ivan Gerling, Ph.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-ivan-gerling-ph-d/) - Investigator Spotlight: April 2014 Ivan Gerling, PhD Dr. Gerling is a professor at the University of Tennessee Health Science Center. Dr. Gerling joined nPOD in 2010. Tell us about your education and background – where are you from, where did you go to school? I was born in Copenhagen, Denmark, and got my initial research
- [Investigator Spotlight: Joe Larkin, Ph.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-joe-larkin-ph-d/) - Investigator Spotlight: May 2014 Joe Larkin, PhD Dr. Larkin is an assistant professor, working with Dr. Maki Nakayama at the University of Florida. Tell us about your education and background – where are you from, where did you go to school? I am originally from Savannah, Georgia. I received a National Merit Scholarship
- [Investigator Spotlight: Decio Eizirik, M.D., D.Med., Ph.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-decio-eizirik-m-d-d-med-ph-d/) - Investigator Spotlight: July 2014 Decio Eizirik, MD, DMed, PhD Dr. Eizirik is a professor at the University Libre de Bruxelles. Dr. Eizirik joined nPOD in 2010. Tell us about your education and background – where are you from, where did you go to school? I was born in Porto Alegre, Brazil; I attended
- [Investigator Spotlight: Margaret Morris, Ph.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-margaret-morris-ph-d/) - Investigator Spotlight: August 2014 Margaret Morris, PhD Dr. Morris is an assistant professor, working with Dr. Jerry Nadler at Eastern Virginia Medical School. Tell us about your education and background – where are you from, where did you go to school? I grew up in Dallas, Texas. I attended the Texas Academy of
- [Investigator Spotlight: Garry Fathman, M.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-garry-fathman-m-d/) - Investigator Spotlight: September 2014 Garry Fathman, MD Dr. Fathman is a professor at Stanford University School of Medicine. Dr. Fathman joined nPOD in 2008. Tell us about your education and background – where are you from, where did you go to school? I grew up in Missouri, initially on a farm along
- [Investigator Spotlight: Marika Bogdani, M.D., Ph.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-marika-bogdani-m-d-ph-d/) - Investigator Spotlight: October 2014 Marika Bogdani, MD, PhD Dr. Bogdani is a research scientist, working with Dr. Thomas Wight at the Benoroya Research Institute. Tell us about your education and background – where are you from, where did you go to school? I grew up in Tirana, Albania, and studied medicine at
- [Investigator Spotlight: Charmaine Simeonovic, Ph.D. & Christopher Parish, Ph.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-charmaine-simeonovic-ph-d-christopher-parish-ph-d/) - Investigator Spotlight: December 2014 Charmaine Simeonovic, PhD & Christopher Parish, PhD Dr. Simeonovic & Dr. Parish joined nPOD in 2010. Tell us about your education and background – where are you from, where did you go to school? Charmaine Simeonovic: I was born Charmaine Joy Tranter (my maiden name) in Lismore, a North Coast country town
- [Investigator Spotlight: Shannon Wallet, Ph.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-shannon-wallet-ph-d/) - Investigator Spotlight: January 2015 Shannon Wallet, PhD Dr. Wallet is an associate professor at the University of Florida. Dr. Wallet joined nPOD in 2012. Tell us about your education and background – where are you from, where did you go to school? I was born and raised in North Carolina where I went to
- [Investigator Spotlight: Matthias von Herrath, M.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-matthias-von-herrath-m-d/) - Investigator Spotlight: February 2015 Matthias von Herrath, MD Dr. Herrath is a professor at the La Jolla Institute and founded the Center for Type 1 Diabetes Research at the La Jolla Institute. Dr. Herrath joined nPOD in 2008. Tell us about your education and background – where are you from, where did you
- [Investigator Spotlight: Lydia Sorokin, Ph.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-lydia-sorkin-ph-d/) - Investigator Spotlight: May 2015 Lydia Sorokin, PhD Dr. Sorokin is a professor of Pathobiochemistry and Director of the Institute of Physiological Chemistry and Pathobiochemistry at the University of Münster. Dr. Sorokin joined nPOD in 2010. Tell us about your education and background – where are you from, where did you go to school? I am from Australia
- [Investigator Spotlight: Noel Morgan, Ph.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-noel-morgan-ph-d/) - Investigator Spotlight: July 2015 Noel Morgan, PhD Dr. Morgan is the Director of the Institute of Biomedical and Clinical Science within Exeter's Medical School. Dr. Morgan joined nPOD in 2010. Tell us about your education and background – where are you from, where did you go to school? I was born and grew up in the Midlands
- [Investigator Spotlight: Abdu Hamad, Ph.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-abdu-hamad-ph-d/) - Investigator Spotlight: September 2015 Abdu Hamad, PhD Dr. Hamad is an associate professor at Johns Hopkins University School of Medicine. Dr. Hamad joined nPOD in 2011. Tell us about your education and background - where are you from, where did you go to school? I am originally from Sudan and am a veterinarian by training. After
- [Investigator Spotlight: Antonio Toniolo, Ph.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-antonio-toniolo-ph-d/) - Investigator Spotlight: October 2015 Antonio Toniolo, PhD Dr. Toniolo is an nPOD investigator at the University of Insubria Medical School in Barese, Italy. Dr. Toniolo joined nPOD in 2011. Tell us about your education and background – where are you from, where did you go to school? I don’t remember a single classmate or friend with diabetes
- [Investigator Spotlight: Richard Oram, MD, PhD](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-richard-oram-md-phd/) - Investigator Spotlight: March 2016 Richard Oram, MD, PhD Dr. Oram is a NIHR Clinical Lecturer at the University of Exeter Medical School, working in clinical nephrology and T1D research. Tell us about your education and background – where are you from, where did you go to school? I’m originally from just south of London in
- [Investigator Spotlight: Richard Lloyd, Ph.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-richard-lloyd-ph-d/) - Investigator Spotlight: November 2014 Richard Lloyd, PhD Dr. Lloyd is a professor at the Baylor College of Medicine. Dr. Lloyd joined nPOD in 2011. Tell us about your education and background – where are you from, where did you go to school? I was raised in Maryland and educated at the University of Maryland,
- [Investigator Spotlight: Heikki Hyoty, M.D., Ph.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-heikki-hyoty-m-d-ph-d/) - Investigator Spotlight: April 2015 Heikki Hyoty, MD, PhD Dr. Hyoty is a professor of Virology at the University of Tampere, Finland. Dr. Hyoty joined nPOD in 2007. Tell us about your education and background – where are you from, where did you go to school? I’m an MD by basic training and completed my PhD in
- [Investigator Spotlight: David Leslie, Ph.D.](https://npod.org/news-events/npod-investigator-spotlights/investigator-spotlight-david-leslie-ph-d/) - Investigator Spotlight: November 2015 David Leslie, PhD Dr. Leslie is a professor and Diabetes Center Lead at the Blizard Institute at the University of London. Dr. Leslie joined nPOD in 2010. Tell us about your education and background – where are you from, where did you go to school? I was born and raised in Dublin,
- [Become an nPOD Partner](https://npod.org/for-partners/become-a-partner/) - Become an nPOD Partner For Organ Procurement Organizations and Tissue Banks The nPOD project relies on its partnerships with Organ Procurement Organizations (OPOs) and Tissue Banks nationwide. If you are an OPO or Tissue Bank Director and are interested in learning more about nPOD, please contact us for more information. We are excited that you
- [Newsletter](https://npod.org/news-events/newsletters/) - nPOD Newsletter Archive 2015 Newsletters April 2015 Investigator Newsletter May 2015 Recovery Partner Newsletter May 2015 Investigator Newsletter July 2015 Investigator Newsletter October 2015 Investigator Newsletter 2014 Newsletters February 2014 March 2014 Recovery Partner Newsletter May 2014 Scientific Newsletter June 2014 Recovery Partner Newsletter July 2014 Investigator Newsletter September 2014 Recovery Partner Newsletter December 2014
- [nPOD Heterogeneity Working Group](https://npod.org/publications/npod-working-groups/npod-h/) - Projects & Publications. nPOD Heterogeneity Working Group More information coming soon.
- [robots.txt check](https://npod.org/robots-txt-check/)
- [Password Request Form Copy](https://npod.org/for-investigators/password-request-form-copy/) - For Investigators. Password Request Form Please complete the form below. [lads_form_errors] Name: Phone number: Email address: Name of Institution: Reason for password request:
- [Organ Recovery Video ](https://npod.org/for-investigators/opo-recovery/organ-recovery-video/) - This instructional video contains graphic content. It is intended for OPO and tissue bank personnel training purposes only. It shows the nPOD recovery procedure from a cadaveric donor. 35:27 minute run time Chapter 1: Introduction. Location = 0:00 seconds into the video Chapter 2: Incision – Opening the Abdomen. Location = 0:38 seconds into the video Chapter 3:
- [1Formatting Examples](https://npod.org/1formatting-examples/) - Heading 1 Heading 1 - used at top of main pages, like About. How We Work. here:https://npod.org//about/how-we-work/ Heading 2 format with headline style Heading 2 with "headline" style gives the white text with green banner. Heading 3 Heading 3 - green text, used for call outs. Heading 4 Heading 4 - bold blue text used
- [Staining Protocols for Human Pancreatic Islets Video](https://npod.org/staining-protocols-for-human-pancreatic-islets-video/) - Staining Protocols for Human Pancreatic Islets Video
- [Pancreas Collection Video](https://npod.org/pancreas-collection-video/) - Pancreas Collection Video
- [2012 nPOD Annual Meeting](https://npod.org/for-partners/2012-npod-annual-meeting/) - For Partners. 2012 nPOD Annual Meeting Thank you to all who participated in the 2012 nPOD Annual Meeting. We are so grateful for the outstanding attendance and participation, which made this years meeting a success. Please see below for more information on the science that was presented on January 16th and 17th, 2012. Abstracst are
- [2012 nPOD Annual Meeting Presentations for Distribution](https://npod.org/for-investigators/2012-npod-annual-meeting-presentations-for-distribution/) - For Investigators. 2012 nPOD Annual Meeting Presentations for Distribution In order to view any of ther presentations below, you must be an approved nPOD investigator and you will need to input the username and password distributed to you for the purpose of viewing investigator documents. Session 1: Viral Etiology Title: Replication Defective Enterovirus Infections: Implications
- [Password Request Form: Success](https://npod.org/password-request-form-success/) - Thank You Thank you for your submission. You can expect to receive a response from nPOD within 2 business days.
- [Site Map](https://npod.org/site-map/)
- [Partner Spotlight: Gift of Life Donor Program, Philadelphia](https://npod.org/for-partners/partner-spotlight-gift-of-life-donor-program-philadelphia/) - For Partners. Gift of Life Donor Program, Philadelphia Gift of Life Donor Program, the nonprofit organization serving the eastern half of Pennsylvania, southern New Jersey and Delaware is one of the oldest and largest of 58 organ procurement organizations (OPO) in the United States. Serving as the link between patients awaiting organ and tissue transplants
- [Partner Spotlight: LifeLink Foundation](https://npod.org/partner-spotlight-lifelink-foundation/) - For Partners. LifeLink Foundation LifeLink Foundation includes three organ recovery organizations: LifeLink of Florida, serving west and southwest Florida; LifeLink of Georgia, serving the state of Georgia; and LifeLink of Puerto Rico, serving Puerto Rico and the U.S. Virgin Islands. This organization also houses the LifeLink Tissue Bank, which makes tens of thousands of tissue grafts
- [DataShare](https://npod.org/for-investigators/datashare/)

## Event Abstracts

- [Pancreatic Islet Size and Endocrine Cell Distribution In the Natural History of Type 1 Diabetes](https://npod.org/event_abstract/pancreatic-islet-size-and-endocrine-cell-distribution-in-the-natural-history-of-type-1-diabetes/)
- [Unmasking Islet Profiles: the link between HLA-I Expression, Immune Infiltration, and Insulin Dynamics in Type 1 Diabetes](https://npod.org/event_abstract/unmasking-islet-profiles-the-link-between-hla-i-expression-immune-infiltration-and-insulin-dynamics-in-type-1-diabetes/)
- [Spatial interrogation of the diabetic hallmarks and tissue composition of human pancreas in 2D and 3D using deep learning models](https://npod.org/event_abstract/spatial-interrogation-of-the-diabetic-hallmarks-and-tissue-composition-of-human-pancreas-in-2d-and-3d-using-deep-learning-models/)
- [Integrated histopathology of the human pancreas throughout stages of type 1 diabetes progression](https://npod.org/event_abstract/integrated-histopathology-of-the-human-pancreas-throughout-stages-of-type-1-diabetes-progression/)
- [The role of CD226 in CD8+ T cells in human type 1 diabetes](https://npod.org/event_abstract/the-role-of-cd226-in-cd8-t-cells-in-human-type-1-diabetes/)
- [Stem cell-derived pancreatic β-cells overexpressing CD155 demonstrate enhanced protection from killing by antigen-specific T cells](https://npod.org/event_abstract/stem-cell-derived-pancreatic-β-cells-overexpressing-cd155-demonstrate-enhanced-protection-from-killing-by-antigen-specific-t-cells/)
- [Public B cell clones are found in the peripheral blood and pancreatic lymph nodes of T1D donors](https://npod.org/event_abstract/public-b-cell-clones-are-found-in-the-peripheral-blood-and-pancreatic-lymph-nodes-of-t1d-donors/)
- [Islet specific T cells from individuals with T1D display a distinctive pro-inflammatory phenotype that may be the result of FOXP3 Treg instability](https://npod.org/event_abstract/islet-specific-t-cells-from-individuals-with-t1d-display-a-distinctive-pro-inflammatory-phenotype-that-may-be-the-result-of-foxp3-treg-instability/)
- [Efferocytosis pathways in islet myeloid cells suppress autoimmunity during type 1 diabetes](https://npod.org/event_abstract/efferocytosis-pathways-in-islet-myeloid-cells-suppress-autoimmunity-during-type-1-diabetes/)
- [Pancreatic stellate cells may contribute to reduced glucose-stimulated insulin secretion in type 1 diabetes](https://npod.org/event_abstract/pancreatic-stellate-cells-may-contribute-to-reduced-glucose-stimulated-insulin-secretion-in-type-1-diabetes/)
- [Functional heterogeneity of alpha cells](https://npod.org/event_abstract/functional-heterogeneity-of-alpha-cells/)
- [Chromogranins and secretogranins in the pathogenesis of type 1 diabetes](https://npod.org/event_abstract/chromogranins-and-secretogranins-in-the-pathogenesis-of-type-1-diabetes/)
- [β cell heterogeneity in the acute IFN-α response](https://npod.org/event_abstract/β-cell-heterogeneity-in-the-acute-ifn-α-response/)
- [Spatial transcriptomic analysis of ductal remodeling in FFPE pancreatic samples from type 1 diabetic, autoantibody positive, and non-diabetic donors](https://npod.org/event_abstract/spatial-transcriptomic-analysis-of-ductal-remodeling-in-ffpe-pancreatic-samples-from-type-1-diabetic-autoantibody-positive-and-non-diabetic-donors/)
- [GABA is crucial for maintaining proper Ca2+ oscillation behavior and insulin secretion in response to glucose stimulation.](https://npod.org/event_abstract/gaba-is-crucial-for-maintaining-proper-ca2-oscillation-behavior-and-insulin-secretion-in-response-to-glucose-stimulation/)
- [Solving the controversy of GLP-1 expression in human alpha cells upon PC1/3 mis-expression in T1D by in situ single-cell analysis](https://npod.org/event_abstract/solving-the-controversy-of-glp-1-expression-in-human-alpha-cells-upon-pc1-3-mis-expression-in-t1d-by-in-situ-single-cell-analysis/)
- [Discovering Novel Therapeutic Approaches for Human Beta Cell Replication Using Insulinomas as Model](https://npod.org/event_abstract/discovering-novel-therapeutic-approaches-for-human-beta-cell-replication-using-insulinomas-as-model/)
- [iPSC-derived islet-like organoids are a tool to study β cell antigen presentation and human autoantigens](https://npod.org/event_abstract/ipsc-derived-islet-like-organoids-are-a-tool-to-study-β-cell-antigen-presentation-and-human-autoantigens/)
- [CaMPARI2 provides a marker of beta-cell function, allowing high content analysis](https://npod.org/event_abstract/campari2-provides-a-marker-of-beta-cell-function-allowing-high-content-analysis/)
- [Ethnic variations in pancreatic weight](https://npod.org/event_abstract/ethnic-variations-in-pancreatic-weight/)
- [Heterogeneity of β Cell extracellular vesicles in T1D: PD-L1 and HLA class I mediated immunomodulation](https://npod.org/event_abstract/heterogeneity-of-β-cell-extracellular-vesicles-in-t1d-pd-l1-and-hla-class-i-mediated-immunomodulation/)
- [Are we missing something? Do the small things matter? Loss of extra-islet beta cells in Type 1 diabetes](https://npod.org/event_abstract/are-we-missing-something-do-the-small-things-matter-loss-of-extra-islet-beta-cells-in-type-1-diabetes/)
- [INS genetic variation promotes type 1 diabetes risk by increasing beta cell insulin production, hyperactivity, and fragility](https://npod.org/event_abstract/ins-genetic-variation-promotes-type-1-diabetes-risk-by-increasing-beta-cell-insulin-production-hyperactivity-and-fragility/)
- [Mapping the human pancreas by imaging mass cytometry reveals early dysregulation in the endocrine and immune compartment in type 1 diabetes progression](https://npod.org/event_abstract/mapping-the-human-pancreas-by-imaging-mass-cytometry-reveals-early-dysregulation-in-the-endocrine-and-immune-compartment-in-type-1-diabetes-progression/)
- [High-throughput TCR screening system for linking antigen specificity and phenotype of T cells involved in the pathogenesis of type 1 diabetes](https://npod.org/event_abstract/high-throughput-tcr-screening-system-for-linking-antigen-specificity-and-phenotype-of-t-cells-involved-in-the-pathogenesis-of-type-1-diabetes/)

## Publications

- [Protocol to generate and utilize pancreatic tissue slices to study endocrine and exocrine physiology in situ from mouse and human tissue](https://www.sciencedirect.com/science/article/pii/S2666166723003660?via=ihub#new_tab) - Publication Categories: ORIGINAL Panzer JK, Caicedo A. Protocol to generate and utilize pancreatic tissue slices to study endocrine and exocrine physiology in situ from mouse and human tissue. STAR Protoc. 2023 Jun 30;4(3):102399. Epub ahead of print. PMID: 37392393.
- [Excess pancreatic elastase alters acinar-β cell communication by impairing the mechano-signaling and the PAR2 pathways](https://www.sciencedirect.com/science/article/pii/S1550413123001845?via=ihub#new_tab) - Publication Categories: ORIGINAL Basile G, Vetere A, Hu J, Ijaduola O, Zhang Y, Liu KC, Eltony AM, De Jesus DF, Fukuda K, Doherty G, Leech CA, Chepurny OG, Holz GG, Yun SH, Andersson O, Choudhary A, Wagner BK, Kulkarni RN. Excess pancreatic elastase alters acinar-β cell communication by impairing the mechano-signaling and the PAR2 pathways.
- [Identifying the Right Diagnosis of Diabetes in Death: Unlocking A Key to Understanding Type 1 Diabetes Pathogenesis](https://npod.org/publication/identifying-the-right-diagnosis-of-diabetes-in-death-unlocking-a-key-to-understanding-type-1-diabetes-pathogenesis/) - Publication Categories: ORIGINAL Jacobsen LM, Campbell-Thompson M, Wasserfall C, Atkinson MA, Schatz DA. Identifying the Right Diagnosis of Diabetes in Death: Unlocking A Key to Understanding Type 1 Diabetes Pathogenesis. Diabetes Care. 2026 Jul 22:dci260020. doi: 10.2337/dci26-0020. Epub ahead of print. PMID: 42485182.
- [miR-30d-5p promotes beta cell recovery and immunomodulation in type 1 diabetes](https://npod.org/publication/mir-30d-5p-promotes-beta-cell-recovery-and-immunomodulation-in-type-1-diabetes/) - Publication Categories: ORIGINAL Gomez-Muñoz L, Perna-Barrull D, Klein D, Alvarez-Cubela S, Godoy-Tena G, Cook DA, Voto-Bernales CQ, Doke M, Murillo M, Valls A, Pastori RL, Dominguez-Bendala J, Vives-Pi M. miR-30d-5p promotes beta cell recovery and immunomodulation in type 1 diabetes. Front Endocrinol (Lausanne). 2026 Jun 2;17:1838666. doi: 10.3389/fendo.2026.1838666. PMID: 42375328; PMCID: PMC13311865.
- [A mitochondrial ROS-dependent antiviral response promotes β cell resilience and is diminished in donors with type 1 diabetes](https://npod.org/publication/a-mitochondrial-ros-dependent-antiviral-response-promotes-β-cell-resilience-and-is-diminished-in-donors-with-type-1-diabetes/) - Publication Categories: ORIGINAL Wagner LE, Melnyk O, Muncy AN, Turner A, Duffett BE, Muralidharan C, Martinez-Irizarry MM, Arvin MC, Orr KS, Wu W, Manduchi E, Quesada-Masachs E, Piganelli JD, Kaestner KH, Brozinick JT, Linnemann AK. A mitochondrial ROS-dependent antiviral response promotes β cell resilience and is diminished in donors with type 1 diabetes. Sci Transl
- [Syntaxin-4, a key exocytosis mediating protein, shows heterogeneous expression in insulin-positive cells of human donors with new-onset and longer duration of type 1 diabetes: comparison with non-diabetic autoantibody-positive and -negative donors](https://npod.org/publication/syntaxin-4-a-key-exocytosis-mediating-protein-shows-heterogeneous-expression-in-insulin-positive-cells-of-human-donors-with-new-onset-and-longer-duration-of-type-1-diabetes-comparison-with-non-diab/) - Publication Categories: ORIGINAL Potman J, Sun KX, Pala K, Krogvold L, Dahl-Jørgensen K, Reddy S. Syntaxin-4, a key exocytosis mediating protein, shows heterogeneous expression in insulin-positive cells of human donors with new-onset and longer duration of type 1 diabetes: comparison with non-diabetic autoantibody-positive and -negative donors. J Mol Histol. 2026 Jul 30;57(4):252. doi: 10.1007/s10735-026-10901-4. PMID:
- [AI-guided analysis of human pancreatic islet sociology reveals distinct cell compositional changes in type 1 diabetes](https://npod.org/publication/ai-guided-analysis-of-human-pancreatic-islet-sociology-reveals-distinct-cell-compositional-changes-in-type-1-diabetes/) - Publication Categories: ORIGINAL Ward C, Banks-Tibbs T, Thorpe H, Giles AM, Mabry SJ, Liu JJ, Chang HC, Yang J, Carney AF, Shaikh HM, Woolley LM, Joseph PN, Kozel J, Rocha EM, Rutter GA, Tseng GC, Liu S, Pless LL, Freyberg Z. AI-guided analysis of human pancreatic islet sociology reveals distinct cell compositional changes in type
- [Beta cell reactivity defines disease-relevant pancreatic CD8 T cells in type 1 diabetes](https://npod.org/publication/beta-cell-reactivity-defines-disease-relevant-pancreatic-cd8-t-cells-in-type-1-diabetes/) - Publication Categories: ORIGINAL Anderson AM, Landry LG, Barra JM, Wells KL, Shilleh AH, Castro-Gutierrez R, Ladd AM, DeNicola M, Babon JAB, Mallone R, Kent SC, Michels AW, Russ HA, Nakayama M. Beta cell reactivity defines disease-relevant pancreatic CD8 T cells in type 1 diabetes. bioRxiv [Preprint]. 2026 Jun 10:2026.06.08.729940. doi: 10.64898/2026.06.08.729940. PMID: 42327043; PMCID: PMC13277807.
- [Single-nucleus transcriptomic analysis of pediatric pancreas reveals cellular heterogeneity and early neoplasia signatures during chronic pancreatitis](https://npod.org/publication/single-nucleus-transcriptomic-analysis-of-pediatric-pancreas-reveals-cellular-heterogeneity-and-early-neoplasia-signatures-during-chronic-pancreatitis/) - Publication Categories: ORIGINAL Ahmed F, Xie X, Dixit A, Moreno-Fernandez ME, Patel EH, Gurria J, Khoury K, Christian P, Bottino R, Kumaragurubaran R, Adeleke D, Wasserfall CH, Wang Y, Abu-El-Haijia M. Single-nucleus transcriptomic analysis of pediatric pancreas reveals cellular heterogeneity and early neoplasia signatures during chronic pancreatitis. medRxiv [Preprint]. 2026 Jul 4. doi: 10.64898/2026.07.01.26357053. Epub
- [Imaging mass cytometry reveals functional and immunological changes during type 1 diabetes progression in human pancreata](https://npod.org/publication/imaging-mass-cytometry-reveals-functional-and-immunological-changes-during-type-1-diabetes-progression-in-human-pancreata/) - Publication Categories: ORIGINAL Steenbuck N, Damond N, Engler S, Kusmartseva I, Posgai AL, Drotar DM, Williams MD, de Souza N, Brusko TM, Brusko MA, Wasserfall CH, Atkinson MA, Bodenmiller B. Imaging mass cytometry reveals functional and immunological changes during type 1 diabetes progression in human pancreata. Nat Metab. 2026 Jul 2. doi: 10.1038/s42255-026-01559-z. Epub ahead
- [HLA class II mediates type 1 diabetes risk by anti-insulin repertoire selection](https://npod.org/publication/hla-class-ii-mediates-type-1-diabetes-risk-by-anti-insulin-repertoire-selection/) - Publication Categories: ORIGINAL García AR, Paterou A, Lee M, Sławiński H, Ferreira R, Landry LG, Trzupek D, Teyton L, Szypowska A Wicker LS, Nakayama M, Todd JA, Pękalski MŁ. HLA class II mediates type 1 diabetes risk by anti-insulin repertoire selection. bioRxiv [Preprint]. 2021 Sep 6. doi: 10.1101/2021.09.06.458974.
- [LSD1-mediated enhancer silencing attenuates retinoic acid signalling during pancreatic endocrine cell development](https://npod.org/publication/lsd1-mediated-enhancer-silencing-attenuates-retinoic-acid-signalling-during-pancreatic-endocrine-cell-development/) - Publication Categories: ORIGINAL Vinckier NK, Patel NA, Geusz RJ, Wang A, Wang J, Matta I, Harrington AR, Wortham M, Wetton N, Wang J, Jhala US, Rosenfeld MG, Benner CW, Shih HP, Sander M. LSD1-mediated enhancer silencing attenuates retinoic acid signalling during pancreatic endocrine cell development. Nat Commun. 2020 Apr 29;11(1):2082. doi: 10.1038/s41467-020-16017-x. PMID: 32350257; PMCID:
- [BET proteins are required for transcriptional activation of the senescent beta cell secretome in Type 1 Diabetes](https://npod.org/publication/bet-proteins-are-required-for-transcriptional-activation-of-the-senescent-beta-cell-secretome-in-type-1-diabetes/) - Publication Categories: ORIGINAL Thompson PJ, Shah A, Apostolopolou C, Bhushan A. BET proteins are required for transcriptional activation of the senescent beta cell secretome in Type 1 Diabetes. bioRxiv [Preprint]. 2019 Aug 15. doi: 10.1101/736231. Update in: Int J Mol Sci. 2019 Sep 26. doi: 10.3390/ijms20194776.
- [GLP-1R is downregulated in beta cells of NOD mice and T1D patients](https://npod.org/publication/glp-1r-is-downregulated-in-beta-cells-of-nod-mice-and-t1d-patients/) - Publication Categories: ORIGINAL Recino A, Barkan K, Schmidt-Christensen A, Nilsson J, Holmes N, Howie D, Holmberg D, Larsson P, Flodström-Tullberg M, Laraia L, Spring DR, Hecksher-Sørensen J, Cooke A, Ladds G, Wållberg M. GLP-1R is downregulated in beta cells of NOD mice and T1D patients. bioRxiv [Preprint]. 2019 Nov 17. doi: 10.1101/845776.
- [Fibroblast activation protein enzyme deficiency prevents liver steatosis, insulin resistance and glucose intolerance and increases fibroblast growth factor-21 in diet induced obese mice](https://npod.org/publication/fibroblast-activation-protein-enzyme-deficiency-prevents-liver-steatosis-insulin-resistance-and-glucose-intolerance-and-increases-fibroblast-growth-factor-21-in-diet-induced-obese-mice/) - Publication Categories: ORIGINAL Chowdhury S, Song S, Zhang HE, Wang M, Gall MG, Yu DMT, Lay AJ, Xiang MSW, Evans KA, Wetzel S, Liu Y, Yau B, Coppage AL, Lo L, Stokes RA, Hawthorne WJ, Cooney GJ, McLennan SV, Gunton JE, Bachovchin WW, Turner N, Kebede MA, McCaughan GW, Twigg SM, Gorrell MD. Fibroblast activation
- [OCA-B/Pou2af1 Expression in T Cells Promotes PD-1 Blockade-Induced Autoimmunity but is Dispensable for Anti-Tumor Immunity](https://npod.org/publication/oca-b-pou2af1-expression-in-t-cells-promotes-pd-1-blockade-induced-autoimmunity-but-is-dispensable-for-anti-tumor-immunity/) - Publication Categories: ORIGINAL Du J, Manna AK, Hughes EP, Bisoma P, Evason KJ, Young A, Wilson WD, Farahat AA, Tantin D. OCA-B/Pou2af1 Expression in Mouse T Cells Promotes PD-1 Blockade-Induced Autoimmunity but is Dispensable for Anti-Tumor Immunity. bioRxiv [Preprint]. 2025 Oct 23:2025.10.22.683978. doi: 10.1101/2025.10.22.683978. PMID: 41279867; PMCID: PMC12633460.
- [Aberrant immune regulation and enrichment of stem-like CD8+ T cells in the pancreatic lymph node during type 1 diabetes development](https://npod.org/publication/aberrant-immune-regulation-and-enrichment-of-stem-like-cd8-t-cells-in-the-pancreatic-lymph-node-during-type-1-diabetes-development/) - Publication Categories: ORIGINAL Peters LD, Seay HR, Smith J, Posgai AL, Berkowitz R, Wasserfall CH, Atkinson MA, Bacher R, Brusko MA, Brusko TM. Aberrant immune regulation and enrichment of stem-like CD8+ T cells in the pancreatic lymph node during type 1 diabetes development. bioRxiv [Preprint]. 2026 Apr 1:2025.05.23.655848. doi: 10.1101/2025.05.23.655848. PMID: 41959012; PMCID: PMC13060253.
- [Deep learning-powered quantification of endocrine cells and CD3+ T cells in the natural history of type 1 diabetes](https://npod.org/publication/deep-learning-powered-quantification-of-endocrine-cells-and-cd3-t-cells-in-the-natural-history-of-type-1-diabetes/) - Publication Categories: ORIGINAL Kang S, Maya N, Morillo M, Outar M, Posgai AL, Lamb DG, Campbell-Thompson M, Kim S. Deep learning-powered quantification of endocrine cells and CD3+ T cells in the natural history of type 1 diabetes. Diabetologia. 2026 May 7. doi: 10.1007/s00125-026-06742-1. Epub ahead of print. PMID: 42096070.
- [Beta cell microRNAs function as molecular hubs of type 1 diabetes pathogenesis and as biomarkers of diabetes risk](https://npod.org/publication/beta-cell-micrornas-function-as-molecular-hubs-of-type-1-diabetes-pathogenesis-and-as-biomarkers-of-diabetes-risk/) - Publication Categories: ORIGINAL Syed F, Krishnan P, Chang G, Rana J, Langlais SR, Hati S, Yamada K, Lam AK, Talware S, Del Carmen Aquino J, Hagedorn E, Liu X, Sardar R, Liu J, Mirmira RG, Evans-Molina C. Beta cell microRNAs function as molecular hubs of type 1 diabetes pathogenesis and as biomarkers of diabetes risk.
- [Spatial transcriptomics from pancreas and local draining lymph node tissue reveals a lymphotoxin-β signature in human type 1 diabetes](https://npod.org/publication/spatial-transcriptomics-from-pancreas-and-local-draining-lymph-node-tissue-reveals-a-lymphotoxin-β-signature-in-human-type-1-diabetes/) - Publication Categories: ORIGINAL Medina-Serpas MA, Brusko M, Golden GJ, Campbell-Thompson M, Rogers T, Reardon S, Posgai AL, Bacher R, Luning Prak ET, Liu C, Kaestner KH, Naji A, Betts MR, McIntyre LM, Atkinson MA, Brusko TM. Spatial transcriptomics from pancreas and local draining lymph node tissue reveals a lymphotoxin-β signature in human type 1 diabetes.
- [Beta cell–targeted PD-1 agonist inhibits cell-mediated autoimmunity in pancreas tissue slices](https://npod.org/publication/beta-cell-targeted-pd-1-agonist-inhibits-cell-mediated-autoimmunity-in-pancreas-tissue-slices/) - Publication Categories: ORIGINAL Becker MW, Brown ME, Wiseman K, Chiodetti AL, Huber MK, Cuaycal AE, Sintara P, Ferreira SM, Smurlick D, Barra JM, Ladd AM, Drotar DM, Atkinson MA, Weber P, Al-Mossawi H, Russ HA, Mahon TM, Brusko TM, Bossi G, Phelps EA. Beta cell-targeted PD-1 agonist inhibits cell-mediated autoimmunity in pancreas tissue slices. Sci
- [Single-cell multiome and spatial profiling reveals pancreas cell type-specific gene regulatory programs driving type 1 diabetes progression](https://npod.org/publication/single-cell-multiome-and-spatial-profiling-reveals-pancreas-cell-type-specific-gene-regulatory-programs-driving-type-1-diabetes-progression/) - Publication Categories: ORIGINAL Melton R, Jimenez S, Elison W, Tucciarone L, Howell A, Wang G, Berti D, Beebe E, Miller M, Zeng C, McGrail C, VanderStel K, Korgaonkar K, Elgamal R, Mummey H, Chiou J, Griffin E, Kusmartseva I, Atkinson M, Preissl S, Theis FJ, Sander M, Gaulton KJ. Single-cell multiome and spatial profiling reveals
- [ACE2 and SARS-CoV-2 Expression in the Normal and COVID-19 Pancreas](https://npod.org/publication/ace2-and-sars-cov-2-expression-in-the-normal-and-covid-19-pancreas/) - Publication Categories: ORIGINAL Kusmartseva I. Wu W, Syed F, Van Der Heide V, Jorgensen M, Joseph P, Tang X, Candelario-Jalil E, Yang C, Nick H, Harbert JL, Posgai A, Lloyd R, Cechin S, Pugliese A, Campbell-Thompson M, Vander Heide RS, Evans-Molina C, Homann D, Atkinson MA. ACE2 and SARS-CoV-2 Expression in the Normal and COVID-19
- [Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry](https://npod.org/publication/single-cell-analysis-of-the-human-pancreas-in-type-2-diabetes-using-multi-spectral-imaging-mass-cytometry/) - Publication Categories: ORIGINAL Wu M, Lee MYY, Bahl V, Traum D, Schug J, Kusmartseva I, Atkinson MA, Fan G; HPAP Consortium; Kaestner KH. Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry. Cell Rep. 2021 Nov 2;37(5):109919. doi: 10.1016/j.celrep.2021.109919. PMID: 34731614; PMCID: PMC8609965.
- [Insulin is expressed by enteroendocrine cells during human fetal development](https://npod.org/publication/insulin-is-expressed-by-enteroendocrine-cells-during-human-fetal-development/) - Publication Categories: ORIGINAL Egozi A, Llivichuzhca-Loja D, McCourt BT, Bahar Halpern K, Farack L, An X, Wang F, Chen K, Konnikova L, Itzkovitz S. Insulin is expressed by enteroendocrine cells during human fetal development. Nat Med. 2021 Dec;27(12):2104-2107. doi: 10.1038/s41591-021-01586-1. Epub 2021 Dec 9. PMID: 34887578.
- [Improving stem cell-derived pancreatic islets using single-cell multi-inferred regulomes](https://npod.org/publication/improving-stem-cell-derived-pancreatic-islets-using-single-cell-multi-inferred-regulomes/) - Publication Categories: COMMENT Rubin de Celis MF, Bonner-Weir S. Improving stem cell-derived pancreatic islets using single-cell multi-inferred regulomes. Dev Cell. 2023 May 8;58(9):725-726. doi: 10.1016/j.devcel.2023.04.008. PMID: 37160100.
- [Redox Regulation of m6A Methyltransferase METTL3 in Human β-cells Controls the Innate Immune Response in Type 1 Diabetes](https://npod.org/publication/redox-regulation-of-m6a-methyltransferase-mettl3-in-human-β-cells-controls-the-innate-immune-response-in-type-1-diabetes/) - Publication Categories: ORIGINAL De Jesus DF, Zhang Z, Brown NK, Li X, Xiao L, Hu J, Gaffrey MJ, Fogarty G, Kahraman S, Wei J, Basile G, Rana TM, Mathews C, Powers AC, Parent AV, Atkinson MA, Dhe-Paganon S, Eizirik DL, Qian WJ, He C, Kulkarni RN. Redox regulation of m6A methyltransferase METTL3 in β-cells controls
- [FOXP3+ regulatory T cells use heparanase to access IL-2 bound to ECM in inflamed tissues](https://npod.org/publication/foxp3-regulatory-t-cells-use-heparanase-to-access-il-2-bound-to-ecm-in-inflamed-tissues/) - Publication Categories: ORIGINAL Martinez HA, Koliesnik I, Kaber G, Reid JK, Nagy N, Barlow G, Falk BA, Medina CO, Hargil A, Vlodavsky I, Li JP, Pérez-Cruz M, Tang SW, Meyer EH, Wrenshall LE, Lord JD, Garcia KC, Palmer TD, Steinman L, Nepom GT, Wight TN, Bollyky PL, Kuipers HF. FOXP3+ regulatory T cells use heparanase to
- [Inhibition of hyaluronan synthesis prevents β-cell loss in obesity-associated type 2 diabetes](https://npod.org/publication/inhibition-of-hyaluronan-synthesis-prevents-β-cell-loss-in-obesity-associated-type-2-diabetes/) - Publication Categories: ORIGINAL Nagy N, Kaber G, Sunkari VG, Marshall PL, Hargil A, Kuipers HF, Ishak HD, Bogdani M, Hull RL, Grandoch M, Fischer JW, McLaughlin TL, Wight TN, Bollyky PL. Inhibition of hyaluronan synthesis prevents β-cell loss in obesity-associated type 2 diabetes. Matrix Biol. 2023 Nov;123:34-47. doi: 10.1016/j.matbio.2023.09.003. Epub 2023 Sep 30. PMID: 37783236;
- [Integrated histopathology of the human pancreas throughout stages of type 1 diabetes progression](https://npod.org/publication/integrated-histopathology-of-the-human-pancreas-throughout-stages-of-type-1-diabetes-progression/) - Publication Categories: ORIGINAL van der Heide V, McArdle S, Nelson MS, Cerosaletti K, Gnjatic S, Mikulski Z, Posgai AL, Kusmartseva I, Atkinson M, Homann D. Integrated histopathology of the human pancreas throughout stages of type 1 diabetes progression. bioRxiv [Preprint]. 2025 Mar 19:2025.03.18.644000. doi: 10.1101/2025.03.18.644000. PMID: 40166299; PMCID: PMC11956956.
- [Apposed networks of interacting TCRs and BCRs exhibiting mosaicked CDR3 sequences made of fixed junctional motifs](https://npod.org/publication/apposed-networks-of-interacting-tcrs-and-bcrs-exhibiting-mosaicked-cdr3-sequences-made-of-fixed-junctional-motifs/) - Publication Categories: ORIGINAL Ahmed R, Majety N, Chan KC, Giwa A, Heinemann J, Zhang H, Margolick J, Al-Hallaf R, Paul P, Bell DR, Song Y, Lee S, Zhou R, Wolfe RM, Donner T, Jie C, Hamad ARA. Apposed networks of interacting TCRs and BCRs exhibiting mosaicked CDR3 sequences made of fixed junctional motifs. bioRxiv [Preprint].
- [Precision-engineered biomimetics: the human fallopian tube](https://npod.org/publication/precision-engineered-biomimetics-the-human-fallopian-tube/) - Publication Categories: ORIGINAL Crawford AJ, Forjaz A, Bhorkar I, Roy T, Schell D, Queiroga V, Ren K, Kramer D, Bons J, Huang W, Russo GC, Lee MH, Schilling B, Wu PH, Shih IM, Wang TL, Kiemen A, Wirtz D. Precision-engineered biomimetics: the human fallopian tube. bioRxiv [Preprint]. 2023 Jun 7:2023.06.06.543923. doi: 10.1101/2023.06.06.543923. PMID: 37333379; PMCID:
- [m6A mRNA Methylation Regulates Early Pancreatic β-Cell Differentiation](https://npod.org/publication/m6a-mrna-methylation-regulates-early-pancreatic-β-cell-differentiation/) - Publication Categories: ORIGINAL Kahraman S, De Jesus DF, Wei J, Brown NK, Zou Z, Hu J, He C, Kulkarni RN. m6A mRNA Methylation Regulates Early Pancreatic β-Cell Differentiation. bioRxiv [Preprint]. 2023 Aug 3:2023.08.03.551675. doi: 10.1101/2023.08.03.551675. Update in: EMBO J. 2024 Nov;43(22):5445-5468. doi: 10.1038/s44318-024-00213-2. PMID: 37577492; PMCID: PMC10418275.
- [Impaired islet function with normal exocrine enzyme secretion is consistent across the head, body, and tail pancreas regions in type 1 diabetes](https://npod.org/publication/impaired-islet-function-with-normal-exocrine-enzyme-secretion-is-consistent-across-the-head-body-and-tail-pancreas-regions-in-type-1-diabetes/) - Publication Categories: ORIGINAL Drotar DM, Mojica-Avila AK, Bloss DT, Cohrs CM, Manson CT, Posgai AL, Williams MD, Brusko MA, Phelps EA, Wasserfall CH, Speier S, Atkinson MA. Impaired islet function with normal exocrine enzyme secretion is consistent across the head, body, and tail pancreas regions in type 1 diabetes. bioRxiv [Preprint]. 2024 Feb 12:2024.02.08.579175. doi:
- [Autoimmune interactions between the HLA-DQβ157 polymorphism, T cell receptors, and microbial mimics of insulin in type 1 diabetes](https://npod.org/publication/autoimmune-interactions-between-the-hla-dqβ157-polymorphism-t-cell-receptors-and-microbial-mimics-of-insulin-in-type-1-diabetes/) - Publication Categories: ORIGINAL García AR, Paterou A, Doherty RDP, Landry LG, Lee M, Anderson AM, Scudder CL, Slawinski H, Ferreira RC, Trzupek D, Szypowska A, Teyton L, Ternette N, Nakayama M, Wicker LS, Todd JA, Pekalski ML. Autoimmune interactions between the HLA-DQβ157 polymorphism, T cell receptors, and microbial mimics of insulin in type 1 diabetes.
- [Proteomic and mass spectrometry-based identification of viral proteins in human tissue samples from the Network for Pancreatic Organ Donors with Diabetes](https://npod.org/publication/proteomic-and-mass-spectrometry-based-identification-of-viral-proteins-in-human-tissue-samples-from-the-network-for-pancreatic-organ-donors-with-diabetes/) - Publication Categories: ORIGINAL Burch TC, Hitefield NL, Morris MA, Pugliese A, Nadler JL, Nyalwidhe JO, JDRF nPOD-Virus Group. Proteomic and mass spectrometry-based identification of viral proteins in human tissue samples from the Network for Pancreate Organ Donors with Diabetes. medrxiv [Preprint]. 2024 Oct 27. doi: 10.1101/2024.10.24.24315944.
- [ENTPD3-specific CAR regulatory T cells for local immune control in T1D](https://npod.org/publication/entpd3-specific-car-regulatory-t-cells-for-local-immune-control-in-t1d/) - Publication Categories: ORIGINAL Pieper T, Riet T, Saetzler V, Bergerhoff K, McGovern J, Delsing L, Atkinson M, Lodie T, Jermutus L, Noyan F, Hust M, Kusmartseva I, Hagedorn M, Lieber M Henschel P, Glaser V, Geffers R, Yang M, Polansky-Biskup J, Eiz-Vesper B, Bonifacius A, Llordella MM, Henry L, Penston D, Gavriil A, Grothier T,
- [3D multi-omic mapping of whole nondiseased human fallopian tubes at cellular resolution reveals a large incidence of ovarian cancer precursors](https://npod.org/publication/3d-multi-omic-mapping-of-whole-nondiseased-human-fallopian-tubes-at-cellular-resolution-reveals-a-large-incidence-of-ovarian-cancer-precursors/) - Publication Categories: ORIGINAL Forjaz A, Queiroga Y, Hernandez A, Crawford A, Qin X, Zhong M, Tsaoatsis M, Joshi S, Kramer D, Nizet O, Bea H, Li Y, Qin S, O'Flynn R, Yang M, Pratt B, Wu F, Gensbigler P, Blecher M, Wu PH, Kagohara LT, Shih lM, Zwicker D, Atkinson M, Shi L, Fan R,
- [A novel disease mechanism leading to the expression of a disallowed gene in the pancreatic beta-cell identified by non-coding, regulatory mutations controlling HK1](https://npod.org/publication/a-novel-disease-mechanism-leading-to-the-expression-of-a-disallowed-gene-in-the-pancreatic-beta-cell-identified-by-non-coding-regulatory-mutations-controlling-hk1/) - Publication Categories: ORIGINAL Wakeling MN, Owens NDL, Hopkinson JR, Johnson MB, Houghton JAL, Dastamani A, Flaxman CS, Wyatt RC, Hewat TI, Hopkins JJ, Laver TW, Heugten RV, Weedon MN, Franco ED, Patel KA, Ellard S, Morgan NG, Cheesman E, Banerjee I, Hattersley AT, Dunne MJ, International Congenital Hyperinsulinism Consortium, Richardson SJ, Flanagan SE. A novel
- [Small things matter: Lack of extra-islet beta cells in Type 1 diabetes](https://npod.org/publication/small-things-matter-lack-of-extra-islet-beta-cells-in-type-1-diabetes/) - Publication Categories: ORIGINAL Murrall K, Luckett T, Lekka C, Flaxman CS, Wyatt R, Akhbari P, Kusmartseva I, Hunter SL, Leete P, Burn I, Osokina E; EXE-T1D Consortium; Shaw JAM, Morgan NG, Richardson SJ. Small things matter: Lack of extraislet β cells in type 1 diabetes. Sci Adv. 2025 Nov 14;11(46):eadz2251. doi: 10.1126/sciadv.adz2251. Epub 2025 Nov
- [Integration of nuclear morphology and 3D imaging to profile cellular neighborhoods](https://npod.org/publication/integration-of-nuclear-morphology-and-3d-imaging-to-profile-cellular-neighborhoods/) - Publication Categories: ORIGINAL Forjaz A, Kramer D, Shen Y, Bea H, Tsapatsis M, Ping J, Queiroga V, San KH, Joshi S, Grubel C, Beery ML, Kusmartseva I, Atkinson M, Kiemen AL, Wirtz D. Integration of nuclear morphology and 3D imaging to profile cellular neighborhoods. bioRxiv [Preprint]. 2025 Apr 4:2025.03.31.646356. doi: 10.1101/2025.03.31.646356. PMID: 40236208; PMCID: PMC11996441.
- [Stressed β-cells contribute to loss of peri-islet extracellular matrix in type 1 diabetes](https://npod.org/publication/stressed-β-cells-contribute-to-loss-of-peri-islet-extracellular-matrix-in-type-1-diabetes/) - Publication Categories: ORIGINAL Johansen CG, Lam K, Farnsworth NL. Stressed β-cells contribute to loss of peri-islet extracellular matrix in type 1 diabetes. bioRxiv [Preprint]. 2025 Jun 27:2025.06.25.661601. doi: 10.1101/2025.06.25.661601. Update in: Front Endocrinol (Lausanne). 2025 Oct 10;16:1675043. doi: 10.3389/fendo.2025.1675043. PMID: 40667128; PMCID: PMC12262395.
- [Efficient transduction of pancreas tissue slices with genetically encoded calcium integrators](https://npod.org/publication/efficient-transduction-of-pancreas-tissue-slices-with-genetically-encoded-calcium-integrators/) - Publication Categories: ORIGINAL Lazimi CS, Stis AE, Panzer JK, Hiller H, Beery ML, Linnemann AK, Stabler CL, Mathews CE, Phelps EA. Efficient transduction of pancreas tissue slices with genetically encoded calcium integrators. bioRxiv [Preprint]. 2025 Sep 16:2025.03.21.644659. doi: 10.1101/2025.03.21.644659. PMID: 40196483; PMCID: PMC11974670.
- [Mapping histologic and functional maturation of human endocrine pancreas across early postnatal periods](https://npod.org/publication/mapping-histologic-and-functional-maturation-of-human-endocrine-pancreas-across-early-postnatal-periods/) - Publication Categories: ORIGINAL Saunders DC, Hart N, Pan FC, Reihsmann CV, Hopkirk AL, Izmaylov N, Mei S, Sherrod BA, Davis C, Duryea J, Haliyur R, Aramandla R, Durai H, Poffenberger G, Martin A, Posgai AL, Kusmartseva I, Beery ML, Yang M, Kang H, Greiner DL, Shultz LD, Cartailler JP, Aamodt KI, Bottino R, Atkinson MA,
- [Transcriptional control of T cell tissue adaptation and effector function in infants and adults](https://npod.org/publication/transcriptional-control-of-t-cell-tissue-adaptation-and-effector-function-in-infants-and-adults/) - Publication Categories: ORIGINAL Szabo PA, Levitin HM, Connors TJ, Chen D, Jin J, Thapa P, Guyer R, Caron DP, Gray JI, Matsumoto R, Kubota M, Brusko M, Brusko TM, Farber DL, Sims PA. Transcriptional control of T cell tissue adaptation and effector function in infants and adults. bioRxiv [Preprint]. 2025 Feb 6:2025.02.01.636039. doi: 10.1101/2025.02.01.636039. PMID:
- [Protection of β cells against pro-inflammatory cytokine stress by the GDF15-ERBB2 signaling](https://npod.org/publication/protection-of-β-cells-against-pro-inflammatory-cytokine-stress-by-the-gdf15-erbb2-signaling/) - Publication Categories: ORIGINAL Sarkar S, Syed F, Webb-Robertson BJ, Melchior JT, Chang G, Gritsenko M, Wang YT, Tsai CF, Liu J, Yi X, Cui Y, Eizirik DL, Metz TO, Rewers M, Evans-Molina C, Mirmira RG, Nakayasu ES. Protection of β cells against pro-inflammatory cytokine stress by the GDF15-ERBB2 signaling. medRxiv [Preprint]. 2023 Nov 27:2023.11.27.23298904. doi:
- [Genetic association and machine learning improves discovery and prediction of type 1 diabetes](https://npod.org/publication/genetic-association-and-machine-learning-improves-discovery-and-prediction-of-type-1-diabetes/) - Publication Categories: ORIGINAL McGrail C, Sears TJ, Kudtarkar P, Carter H, Gaulton K. Genetic association and machine learning improves discovery and prediction of type 1 diabetes. medRxiv [Preprint]. 2024 Aug 2:2024.07.31.24311310. doi: 10.1101/2024.07.31.24311310. PMID: 39132494; PMCID: PMC11312647.
- [Identification of a type 1 diabetes-associated T cell receptor repertoire signature from the human peripheral blood](https://npod.org/publication/identification-of-a-type-1-diabetes-associated-t-cell-receptor-repertoire-signature-from-the-human-peripheral-blood/) - Publication Categories: ORIGINAL Rawat P, Shapiro MR, Peters LD, Widrich M, Mayer-Blackwell K, Motwani K, Pavlović M, Al Hajj G, Posgai AL, Kanduri C, Isacchini G, Chernigovskaya M, Scheffer L, Motwani K, Balzano-Nogueira LO, Pettenger-Willey CM, Valkiers S, Jacobsen LM, Haller MJ, Schatz DA, Wasserfall CH, Emerson RO, Fiore-Gartland AJ, Atkinson MA, Klambauer G, Sandve
- [NLTD 2.0: A Nonlinear Framework for Robust and Customizable Color Deconvolution in Histopathology](https://npod.org/publication/nltd-2-0-a-nonlinear-framework-for-robust-and-customizable-color-deconvolution-in-histopathology/) - Publication Categories: ORIGINAL Selaru F, Phillip JM, Wirtz D, Wu PH. NLTD 2.0: A Nonlinear Framework for Robust and Customizable Color Deconvolution in Histopathology. bioRxiv [Preprint]. 2025 Oct 3:2025.10.02.679756. doi: 10.1101/2025.10.02.679756. PMID: 41256483; PMCID: PMC12622035.
- [Inhibition of cell-mediated immunity in type 1 diabetes by beta cell-targeted PD-1 agonists in pancreas tissue slices](https://npod.org/publication/inhibition-of-cell-mediated-immunity-in-type-1-diabetes-by-beta-cell-targeted-pd-1-agonists-in-pancreas-tissue-slices/) - Publication Categories: ORIGINAL Becker MW, Brown M, Wiseman K, Chiodetti AL, Huber MK, Cuaycal AE, Sintara P, Ferreira SM, Smurlick D, Barra JM, Ladd AM, Drotar DM, Atkinson MA, Weber P, Al-Mossawi H, Russ HA, Mahon TM, Brusko TM, Bossi G, Phelps EA. Inhibition of cell-mediated immunity in type 1 diabetes by beta cell-targeted PD-1
- [Single-Islet Proteomics Maps Pseudo-Temporal Islet Immune Responses and Dysfunction in Stage 1 Type 1 Diabetes](https://npod.org/publication/single-islet-proteomics-maps-pseudo-temporal-islet-immune-responses-and-dysfunction-in-stage-1-type-1-diabetes/) - Publication Categories: ORIGINAL Kelly S, Sarkar S, Williams SM, Fu AD, Butterworth EA, Sagendorf TJ, Nierves LA, Kwon Y, Li X, Petyuk VA, Chen J, Nakayasu ES, Atkinson MA, Kulkarni RN, Mathews CE, Zhu Y, Campbell-Thompson M, Qian WJ. Single-Islet Proteomics Maps Pseudo-Temporal Islet Immune Responses and Dysfunction in Stage 1 Type 1 Diabetes. bioRxiv
- [Single cell and spatial characterization of the human pancreas reveals drivers of beta cell dysfunction in cystic fibrosis](https://npod.org/publication/single-cell-and-spatial-characterization-of-the-human-pancreas-reveals-drivers-of-beta-cell-dysfunction-in-cystic-fibrosis/) - Publication Categories: ORIGINAL Mummey HM, Corban S, Lucero J, Melton RL, Pittigher M, Johnston VC, Deutsch GH, D'Antonio-Chronowska A, Wang A, Hull-Meichle RL, Gaulton KJ. Single cell and spatial characterization of the human pancreas reveals drivers of beta cell dysfunction in cystic fibrosis. bioRxiv [Preprint]. 2025 Dec 16:2025.12.14.694120. doi: 10.64898/2025.12.14.694120. PMID: 41446239; PMCID: PMC12724667.
- [3D imaging of human pancreas suggests islet size and endocrine composition influence their loss in type 1 diabetes](https://npod.org/publication/3d-imaging-of-human-pancreas-suggests-islet-size-and-endocrine-composition-influence-their-loss-in-type-1-diabetes/) - Publication Categories: ORIGINAL Rippa A, Posgai AL, Currlin S, Brusko M, Williams MD, Kaddis JS, Kusmartseva I, Wasserfall CH, Campbell-Thompson M, Atkinson MA. 3D imaging of human pancreas suggests islet size and endocrine composition influence their loss in type 1 diabetes. Nat Commun. 2025 Dec 11;16(1):11379. doi: 10.1038/s41467-025-66198-6. PMID: 41381473; PMCID: PMC12727770.
- [Circulating C-Peptide Levels in Living Children and Young People and Pancreatic β-Cell Loss in Pancreas Donors Across Type 1 Diabetes Disease Duration](https://npod.org/publication/circulating-c-peptide-levels-in-living-children-and-young-people-and-pancreatic-β-cell-loss-in-pancreas-donors-across-type-1-diabetes-disease-duration/) - Publication Categories: ORIGINAL Carr ALJ, Inshaw JRJ, Flaxman CS, Leete P, Wyatt RC, Russell LA, Palmer M, Prasolov D, Worthington T, Hull B, Wicker LS, Dunger DB, Oram RA, Morgan NG, Todd JA, Richardson SJ, Besser REJ. Circulating C-Peptide Levels in Living Children and Young People and Pancreatic β-Cell Loss in Pancreas Donors Across Type
- [Suppression of Pituitary Hormone Genes in Subjects Who Died From COVID-19 Independently of Virus Detection in the Gland](https://npod.org/publication/suppression-of-pituitary-hormone-genes-in-subjects-who-died-from-covid-19-independently-of-virus-detection-in-the-gland/) - Publication Categories: ORIGINAL Poma AM, Proietti A, Macerola E, Bonuccelli D, Conti M, Salvetti A, Dolo V, Chillà A, Basolo A, Santini F, Toniolo A, Basolo F. Suppression of Pituitary Hormone Genes in Subjects Who Died From COVID-19 Independently of Virus Detection in the Gland. J Clin Endocrinol Metab. 2022 Jul 14;107(8):2243-2253. doi: 10.1210/clinem/dgac312. PMID:
- [β Cell microRNAs Function as Molecular Hubs of Type 1 Diabetes Pathogenesis and as Biomarkers of Diabetes Risk](https://npod.org/publication/β-cell-micrornas-function-as-molecular-hubs-of-type-1-diabetes-pathogenesis-and-as-biomarkers-of-diabetes-risk/) - Publication Categories: ORIGINAL Syed F, Krishnan P, Chang G, Langlais SR, Hati S, Yamada K, Lam AK, Talware S, Liu X, Sardar R, Liu J, Mirmira RG, Evans-Molina C. β Cell microRNAs Function as Molecular Hubs of Type 1 Diabetes Pathogenesis and as Biomarkers of Diabetes Risk. bioRxiv [Preprint]. 2023 Jun 15:2023.06.15.545170. doi: 10.1101/2023.06.15.545170. PMID:
- [Islet cells in human type 1 diabetes: from recent advances to novel therapies - a symposium-based roadmap for future research](https://npod.org/publication/islet-cells-in-human-type-1-diabetes-from-recent-advances-to-novel-therapies-a-symposium-based-roadmap-for-future-research/) - Publication Categories: REVIEW Cantley J, Eizirik DL, Latres E, Dayan CM. Islet cells in human type 1 diabetes: from recent advances to novel therapies - a symposium-based roadmap for future research. J Endocrinol. 2023 Aug 31;259(1):e230082. doi: 10.1530/JOE-23-0082. PMID: 37493471; PMCID: PMC10502961.
- [Enteroviral infections are not associated with type 2 diabetes](https://npod.org/publication/enteroviral-infections-are-not-associated-with-type-2-diabetes/) - Publication Categories: ORIGINAL Liu H, Geravandi S, Grasso AM, Sikdar S, Pugliese A, Maedler K. Enteroviral infections are not associated with type 2 diabetes. Front Endocrinol (Lausanne). 2023 Oct 30;14:1236574. doi: 10.3389/fendo.2023.1236574. PMID: 38027145; PMCID: PMC10643152.
- [Coxsackievirus infection induces direct pancreatic β cell killing but poor antiviral CD8+ T cell responses](https://npod.org/publication/coxsackievirus-infection-induces-direct-pancreatic-β-cell-killing-but-poor-antiviral-cd8-t-cell-responses/) - Publication Categories: ORIGINAL Vecchio F, Carré A, Korenkov D, Zhou Z, Apaolaza P, Tuomela S, Burgos-Morales O, Snowhite I, Perez-Hernandez J, Brandao B, Afonso G, Halliez C, Kaddis J, Kent SC, Nakayama M, Richardson SJ, Vinh J, Verdier Y, Laiho J, Scharfmann R, Solimena M, Marinicova Z, Bismuth E, Lucidarme N, Sanchez J, Bustamante C,
- [The Ailing β-Cell in Diabetes: Insights From a Trip to the ER: The 2023 Outstanding Scientific Achievement Award Lecture](https://npod.org/publication/the-ailing-β-cell-in-diabetes-insights-from-a-trip-to-the-er-the-2023-outstanding-scientific-achievement-award-lecture/) - Publication Categories: ORIGINAL Evans-Molina C. The Ailing β-Cell in Diabetes: Insights From a Trip to the ER: The 2023 Outstanding Scientific Achievement Award Lecture. Diabetes. 2024 Apr 1;73(4):545-553. doi: 10.2337/dbi23-0030. PMID: 38507587; PMCID: PMC10958579.
- [Influence of sphingolipid enzymes on blood glucose levels, development of diabetes, and involvement of pericytes](https://npod.org/publication/influence-of-sphingolipid-enzymes-on-blood-glucose-levels-development-of-diabetes-and-involvement-of-pericytes/) - Publication Categories: ORIGINAL Buschard K, Josefsen K, Krogvold L, Gerling I, Dahl-Jørgensen K, Pociot F. Influence of sphingolipid enzymes on blood glucose levels, development of diabetes, and involvement of pericytes. Diabetes Metab Res Rev. 2024 Mar;40(3):e3792. doi: 10.1002/dmrr.3792. PMID: 38517704.
- [The Human Pancreas in Type 1 Diabetes: Lessons Learned from the Network of Pancreatic Organ Donors with Diabetes](https://npod.org/publication/the-human-pancreas-in-type-1-diabetes-lessons-learned-from-the-network-of-pancreatic-organ-donors-with-diabetes/) - Publication Categories: REVIEW Kusmartseva I, Posgai A, Yang M, Oram R, Atkinson M, Pugliese A, Evans-Molina C. The Human Pancreas in Type 1 Diabetes: Lessons Learned from the Network of Pancreatic Organ Donors with Diabetes. Cold Spring Harb Perspect Med. 2025 Dec 1;15(12):a041588. doi: 10.1101/cshperspect.a041588. PMID: 39134385; PMCID: PMC12667406.
- [Network for Pancreatic Organ donors with Diabetes-Kidney: A Heterogenous Donor Cohort for the Investigation of Diabetic Kidney Disease Pathogenesis and Progression](https://npod.org/publication/network-for-pancreatic-organ-donors-with-diabetes-kidney-a-heterogenous-donor-cohort-for-the-investigation-of-diabetic-kidney-disease-pathogenesis-and-progression/) - Publication Categories: ORIGINAL Ward HH, Anquetil F, Das V, Gibson CB, Dovmark TH, Kusmartseva I, Yang M, Beery M, Atkinson MA, Zeng X, Alpers CE, Wesley JD, Karihaloo A. Network for Pancreatic Organ donors with Diabetes-Kidney: A Heterogenous Donor Cohort for the Investigation of Diabetic Kidney Disease Pathogenesis and Progression. Kidney360. 2025 Jan 1;6(1):15-26. doi:
- [Insulin production is sustained during DNA damage-mediated senescence in adult human beta cells](https://npod.org/publication/insulin-production-is-sustained-during-dna-damage-mediated-senescence-in-adult-human-beta-cells/) - Publication Categories: ORIGINAL Préfontaine C, Pipella J, Rampazzo Morelli N, Chen YC, Verchere CB, Thompson PJ. Insulin production is sustained during DNA damage-mediated senescence in adult human beta cells. Diabetologia. 2026 Feb;69(2):421-435. doi: 10.1007/s00125-025-06603-3. Epub 2025 Nov 13. PMID: 41231256; PMCID: PMC12779684.
- [Characterisation of human pancreatic mesenchymal stromal cells in type 1 diabetes](https://npod.org/publication/characterisation-of-human-pancreatic-mesenchymal-stromal-cells-in-type-1-diabetes/) - Publication Categories: ORIGINAL Dewhurst-Trigg RE, Atkins J, Morgan NG, Eichmann M, Richardson SJ, Rackham CL. Characterisation of human pancreatic mesenchymal stromal cells in type 1 diabetes. Diabetologia. 2025 Dec 21. doi: 10.1007/s00125-025-06634-w. Epub ahead of print. PMID: 41422462.
- [Detection of Enterovirus in Islet Cells of Patients with Type 1 Diabetes: what do we learn from immunohistochemistry? Reply to Hansson SF, Korsgren S, Pontén F et al](https://npod.org/publication/6306/) - Publication Categories: REVIEW Richardson, S.J., Leete, P., Dhayal, S., Russell, MA., Oikarinen, M., Laiho, J., Svedin, S., Lind, K., Rosenling, T., Chapman, N., Bone, A.J., Foulis, A.K., Frisk, G., Flodström-Tullberg, M., Hober, D., Hyoty, H., Pugliese, A., Morgan, N.G. (2014). Detection of enterovirus in islet cells of patients with type 1 diabetes: what do we
- [The Hippo terminal effector YAP boosts enterovirus replication in type 1 diabetes](https://npod.org/publication/the-hippo-terminal-effector-yap-boosts-enterovirus-replication-in-type-1-diabetes/) - Publication Categories: ORIGINAL Geravandi, S., Liu, H., Pahwa, H. et al. The Hippo terminal effector YAP boosts enterovirus replication in type 1 diabetes. Nat Commun 16, 8882 (2025). https://doi.org/10.1038/s41467-025-64508-6
- [Whole tissue spatial cellular analysis reveals increased macrophage infiltration in pancreata of autoantibody positive donors and patients with type 1 diabetes](https://npod.org/publication/whole-tissue-spatial-cellular-analysis-reveals-increased-macrophage-infiltration-in-pancreata-of-autoantibody-positive-donors-and-patients-with-type-1-diabetes/) - Publication Categories: ORIGINAL Quesada-Masachs E, Zilberman S, Chu T, McArdle S, Kiosses WB, Rajendran S, Atkinson MA, Benkahla MA, Mikulski Z, von Herrath M. Whole tissue spatial cellular analysis reveals increased macrophage infiltration in pancreata of autoantibody positive donors and patients with type 1 diabetes. bioRxiv [Preprint]. 2025 Sep 17:2025.09.13.676053. doi: 10.1101/2025.09.13.676053. PMID: 41000615; PMCID:
- [TLR5 influences the development of type 1 diabetes](https://npod.org/publication/tlr5-influences-the-development-of-type-1-diabetes/)
- [Beta cell dysfunction occurs independently of insulitis in type 1 diabetes pathogenesis](https://npod.org/publication/beta-cell-dysfunction-occurs-independently-of-insulitis-in-type-1-diabetes-pathogenesis/) - Publication Categories: ORIGINAL Huber MK, Widener AE, Cuaycal AE, Smurlick D, Butterworth EA, Lenchik NI, Chen J, Beery M, Hiller H, Verney E, Kusmartseva I, Rupnik MS, Campbell-Thompson M, Gerling IC, Atkinson MA, Mathews CE, Phelps EA. Beta cell dysfunction occurs independently of insulitis in type 1 diabetes pathogenesis. Cell Rep. 2025 Aug 26;44(9):116174. doi:
- [Reanalysis of study of pancreatic effects of incretin therapy: methodological deficiencies](https://npod.org/publication/re-analysis-of-study-of-pancreatic-effects-of-incretin-therapy-methodological-deficiencies/) - Publication Categories: REVIEW Bonner-Weir S, In't Veld P, Weir G. (2014) Re-analysis of study of pancreatic effects of incretin therapy: Methodological deficiencies. Diabetes Obes Metab. doi: 10.1111/dom.12257.
- [Increased Proliferation of the Pancreatic Duct Gland Compartment in Type 1 Diabetes](https://npod.org/publication/increased-proliferation-of-the-pancreatic-duct-gland-compartment-in-type-1-diabetes/) - Publication Categories: ORIGINAL Moin AS, Butler PC, Butler AE. (2017) Increased Proliferation of the Pancreatic Duct Gland Compartment in Type 1 Diabetes. J Clin Endocrinol Metab. 2017 Jan 1;102(1):200-209.
- [Insulitis and exocrinitis in autoantibody-positive non-diabetic individuals: role of HLA genotypes](https://npod.org/publication/insulitis-and-exocrinitis-in-autoantibody-positive-non-diabetic-individuals-role-of-hla-genotypes/) - Publication Categories: ORIGINAL Diedisheim M, Mallone R, Pugliese A, Dubois-Laforgue D, Larger E. Insulitis and exocrinitis in autoantibody-positive non-diabetic individuals: role of HLA genotypes. J Clin Endocrinol Metab. 2025 Jan 28:dgaf039. doi: 10.1210/clinem/dgaf039. Epub ahead of print. PMID: 39873482.
- [Beyond the loss of beta cells: a quantitative analysis of islet architecture in adults with and without type 1 diabetes](https://npod.org/publication/beyond-the-loss-of-beta-cells-a-quantitative-analysis-of-islet-architecture-in-adults-with-and-without-type-1-diabetes/) - Publication Categories: ORIGINAL Verschueren van Rees N, Ashwin P, McMullan C, Krogvold L, Dahl-Jørgensen K, Morgan NG, Leete P, Wedgwood KCA. Beyond the loss of beta cells: a quantitative analysis of islet architecture in adults with and without type 1 diabetes. Diabetologia. 2025 May;68(5):1031-1043. doi: 10.1007/s00125-025-06376-9. Epub 2025 Feb 26. PMID: 40011232; PMCID: PMC12021988.
- [Increased inflammation as well as decreased endoplasmic reticulum stress and translation differentiate pancreatic islets from donors with pre-symptomatic stage 1 type 1 diabetes and non-diabetic donors](https://npod.org/publication/increased-inflammation-as-well-as-decreased-endoplasmic-reticulum-stress-and-translation-differentiate-pancreatic-islets-from-donors-with-pre-symptomatic-stage-1-type-1-diabetes-and-non-diabetic-donor/) - Publication Categories: ORIGINAL Swensen AC, Piehowski PD, Chen J, Chan XY, Kelly SS, Petyuk VA, Moore RJ, Nasif L, Butterworth EA, Atkinson MA, Kulkarni RN, Campbell-Thompson M, Mathews CE, Qian WJ. Increased inflammation as well as decreased endoplasmic reticulum stress and translation differentiate pancreatic islets from donors with pre-symptomatic stage 1 type 1 diabetes and
- [Pancreatic β-cell regeneration in situ by the ALK3 agonist THR-123](https://npod.org/publication/pancreatic-β-cell-regeneration-in-situ-by-the-alk3-agonist-thr-123/) - Publication Categories: ORIGINAL Álvarez-Cubela S, Altilio ID, Doke M, Klein D, Tamayo A, Alcázar Ó, Santana CG, Qadir MMF, Alver CG, Cruz F, Biggs O, Tovar Castro JD, Navarro-Rubio B, Ricordi C, Caicedo A, Buchwald P, Agarwal A, Oltra E, Pastori RL, Domínguez-Bendala J. Pancreatic β-cell regeneration in situ by the ALK3 agonist THR-123. Nat
- [The Extra-Islet Pancreas Supports Autoimmunity in Human Type 1 Diabetes](https://npod.org/publication/the-extra-islet-pancreas-supports-autoimmunity-in-human-type-1-diabetes/) - Publication Categories: ORIGINAL Barlow GL, Schürch CM, Bhate SS, Phillips D, Young A, Dong S, Martinez HA, Kaber G, Nagy N, Ramachandran S, Meng J, Korpos E, Bluestone JA, Nolan GP, Bollyky PL. The Extra-Islet Pancreas Supports Autoimmunity in Human Type 1 Diabetes. medRxiv [Preprint]. 2024 Dec 14:2023.03.15.23287145. doi: 10.1101/2023.03.15.23287145. Update in: Elife. 2025 Apr
- [Immune perturbations in human pancreas lymphatic tissues prior to and after type 1 diabetes onset](https://npod.org/publication/immune-perturbations-in-human-pancreas-lymphatic-tissues-prior-to-and-after-type-1-diabetes-onset/) - Publication Categories: ORIGINAL Golden GJ, Wu VH, Hamilton JT, Amses KR, Shapiro MR, Japp AS, Liu C, Pampena MB, Kuri-Cervantes L, Knox JJ, Gardner JS; HPAP Consortium; Atkinson MA, Brusko TM, Prak ETL, Kaestner KH, Naji A, Betts MR. Immune perturbations in human pancreas lymphatic tissues prior to and after type 1 diabetes onset. bioRxiv
- [Detection of enterovirus RNA in pancreas and lymphoid tissues of organ donors with type 1 diabetes](https://npod.org/publication/detection-of-enterovirus-rna-in-pancreas-and-lymphoid-tissues-of-organ-donors-with-type-1-diabetes/) - Publication Categories: ORIGINAL Laiho JE, Oikarinen S, Morfopoulou S, Oikarinen M, Renner A, Depledge D, Ross MC, Gerling IC, Breuer J, Petrosino JF, Plagnol V, Pugliese A, Toniolo A, Lloyd RE, Hyöty H; JDRF nPOD-Virus Group. Detection of enterovirus RNA in pancreas and lymphoid tissues of organ donors with type 1 diabetes. medRxiv [Preprint]. 2024
- [Dysfunctional β-cell longevity in diabetes relies on energy conservation and positive epistasis](https://npod.org/publication/dysfunctional-β-cell-longevity-in-diabetes-relies-on-energy-conservation-and-positive-epistasis/) - Publication Categories: ORIGINAL Raval K, Jamshidi N, Seyran B, Salwinski L, Pillai R, Yang L, Ma F, Pellegrini M, Shin J, Yang X, Tudzarova S. Dysfunctional β-cell longevity in diabetes relies on energy conservation and positive epistasis. Life Sci Alliance. 2024 Sep 23;7(12):e202402743. doi: 10.26508/lsa.202402743. PMID: 39313296; PMCID: PMC11420665.
- [Loss of Insulin-Positive Cell Clusters Precedes the Decrease in Islet Frequency and β-Cell Area in Type 1 Diabetes](https://npod.org/publication/loss-of-insulin-positive-cell-clusters-precedes-the-decrease-in-islet-frequency-and-β-cell-area-in-type-1-diabetes/) - Publication Categories: ORIGINAL Drotar DM, Vazquez Ramos GJA, Williams MD, David ST, Luce C, Smith JA, Posgai AL, Bacher R, Campbell-Thompson M, Kusmartseva I, Brusko MA, Atkinson MA, Wasserfall CH. Loss of Insulin-Positive Cell Clusters Precedes the Decrease in Islet Frequency and β-Cell Area in Type 1 Diabetes. Diabetes. 2025 Jul 24:db250326. doi: 10.2337/db25-0326. Epub
- [The antigen presentation landscape of cytokine-stressed human pancreatic islets](https://npod.org/publication/the-antigen-presentation-landscape-of-cytokine-stressed-human-pancreatic-islets/) - Publication Categories: ORIGINAL Nanaware PP, Calvo-Calle JM, Redick SD, Tarpley MW, Cruz J, Clement CC, Manganaro A, Velarde de la Cruz EE, Muneeruddin K, Faulkner M, Wang JP, Shaffer SA, Harlan DM, Santambrogio L, Kent SC, Stern LJ. The antigen presentation landscape of cytokine-stressed human pancreatic islets. Cell Rep. 2025 Jul 18;44(8):115927. doi: 10.1016/j.celrep.2025.115927. PMID:
- [T cell and autoantibody recognition of nucleus-associated islet autoantigens in individuals with type 1 diabetes](https://npod.org/publication/t-cell-and-autoantibody-recognition-of-nucleus-associated-islet-autoantigens-in-individuals-with-type-1-diabetes/) - Publication Categories: ORIGINAL Guyer P, Seminova K, Lugar M, Manganaro A, Velarde de la Cruz EE, Hartley R, Smithmyer ME, Speake C, Bonifacio E, Kent SC, James EA. T cell and autoantibody recognition of nucleus-associated islet autoantigens in individuals with type 1 diabetes. Diabetologia. 2025 Jun 9. doi: 10.1007/s00125-025-06458-8. Epub ahead of print. PMID: 40490584.
- [Pancreatic exocrine damage induces beta cell stress in zebrafish larvae](https://npod.org/publication/pancreatic-exocrine-damage-induces-beta-cell-stress-in-zebrafish-larvae/) - Publication Categories: ORIGINAL Faraj N, Hoogaars WMH, Duinkerken BHP, Wolters AHG, Kats K, Dekkers MC, Zaldumbide A, Giepmans BNG. Pancreatic exocrine damage induces beta cell stress in zebrafish larvae. Diabetologia. 2025 Apr 28. doi: 10.1007/s00125-025-06432-4. Epub ahead of print. PMID: 40295334.
- [The Cardiovascular Repository for Type 1 Diabetes (CaRe-T1D): An NIDDK Initiative to Advance Understanding of Mechanisms Underlying Cardiovascular Disease in Type 1 Versus Type 2 Diabetes](https://npod.org/publication/the-cardiovascular-repository-for-type-1-diabetes-care-t1d-an-niddk-initiative-to-advance-understanding-of-mechanisms-underlying-cardiovascular-disease-in-type-1-versus-type-2-diabetes/) - Publication Categories: ORIGINAL Jones TLZ, Kusmartseva I, Litovsky S, Thakar R, Posgai AL, Eckel RH, Atkinson MA. The Cardiovascular Repository for Type 1 Diabetes (CaRe-T1D): An NIDDK Initiative to Advance Understanding of Mechanisms Underlying Cardiovascular Disease in Type 1 Versus Type 2 Diabetes. Diabetes. 2025 Apr 24:db250017. doi: 10.2337/db25-0017. Epub ahead of print. PMID: 40272262.
- [Distribution of glutathione peroxidase-1 immunoreactive cells in pancreatic islets from type 1 diabetic donors and non-diabetic donors with and without islet cell autoantibodies is variable and independent of disease](https://npod.org/publication/distribution-of-glutathione-peroxidase-1-immunoreactive-cells-in-pancreatic-islets-from-type-1-diabetic-donors-and-non-diabetic-donors-with-and-without-islet-cell-autoantibodies-is-variable-and-indepe/) - Publication Categories: ORIGINAL Pala K, Sun KX, Krogvold L, Dahl-Jørgensen K, Reddy S. Distribution of glutathione peroxidase-1 immunoreactive cells in pancreatic islets from type 1 diabetic donors and non-diabetic donors with and without islet cell autoantibodies is variable and independent of disease. Cell Tissue Res. 2025 Mar 10. doi: 10.1007/s00441-025-03955-5. Epub ahead of print. PMID:
- [Enterovirus VP1 protein and HLA class I hyperexpression in pancreatic islet cells of organ donors with type 1 diabetes](https://npod.org/publication/enterovirus-vp1-protein-and-hla-class-i-hyperexpression-in-pancreatic-islet-cells-of-organ-donors-with-type-1-diabetes/) - Publication Categories: ORIGINAL Rodriguez-Calvo T, Laiho JE, Oikarinen M, Akhbari P, Flaxman C, Worthington T, Apaolaza P, Kaddis JS, Kusmartseva I, Tauriainen S, Campbell-Thompson M, Atkinson MA, von Herrath M, Hyöty H, Morgan NG, Pugliese A, Richardson SJ; nPOD-Virus group. Enterovirus VP1 protein and HLA class I hyperexpression in pancreatic islet cells of organ donors
- [Joint analysis of the nPOD-Virus Group data: the association of enterovirus with type 1 diabetes is supported by multiple markers of infection in pancreas tissue](https://npod.org/publication/joint-analysis-of-the-npod-virus-group-data-the-association-of-enterovirus-with-type-1-diabetes-is-supported-by-multiple-markers-of-infection-in-pancreas-tissue/) - Publication Categories: ORIGINAL Richardson SJ, Rodriguez-Calvo T, Laiho JE, Kaddis JS, Nyalwidhe JO, Kusmartseva I, Morfopoulou S, Petrosino JF, Plagnol V, Maedler K, Morris MA, Nadler JL, Atkinson MA, von Herrath M, Lloyd RE, Hyoty H, Morgan NG, Pugliese A; nPOD-Virus Group. Joint analysis of the nPOD-Virus Group data: the association of enterovirus with type
- [Interferon-α promotes HLA-B-restricted presentation of conventional and alternative antigens in human pancreatic β-cells](https://npod.org/publication/interferon-α-promotes-hla-b-restricted-presentation-of-conventional-and-alternative-antigens-in-human-pancreatic-β-cells/) - Publication Categories: ORIGINAL Carré A, Samassa F, Zhou Z, Perez-Hernandez J, Lekka C, Manganaro A, Oshima M, Liao H, Parker R, Nicastri A, Brandao B, Colli ML, Eizirik DL, Aluri J, Patel D, Göransson M, Burgos Morales O, Anderson A, Landry L, Kobaisi F, Scharfmann R, Marselli L, Marchetti P, You S, Nakayama M, Hadrup
- [Developmental beta-cell death orchestrates the islet's inflammatory milieu by regulating immune system crosstalk](https://npod.org/publication/developmental-beta-cell-death-orchestrates-the-islets-inflammatory-milieu-by-regulating-immune-system-crosstalk/) - Publication Categories: ORIGINAL Akhtar MN, Hnatiuk A, Delgadillo-Silva L, Geravandi S, Sameith K, Reinhardt S, Bernhardt K, Singh SP, Maedler K, Brusch L, Ninov N. Developmental beta-cell death orchestrates the islet's inflammatory milieu by regulating immune system crosstalk. EMBO J. 2025 Feb;44(4):1131-1153. doi: 10.1038/s44318-024-00332-w. Epub 2025 Jan 6. PMID: 39762647; PMCID: PMC11833124.
- [Automated analysis of ultrastructure through large-scale hyperspectral electron microscopy](https://npod.org/publication/automated-analysis-of-ultrastructure-through-large-scale-hyperspectral-electron-microscopy/) - Publication Categories: ORIGINAL Duinkerken, B.H.P., Alsahaf, A.M.J., Hoogenboom, J.P. et al. Automated analysis of ultrastructure through large-scale hyperspectral electron microscopy. npj Imaging 2, 53 (2024). https://doi.org/10.1038/s44303-024-00059-7
- [G6PC2 controls glucagon secretion by defining the set point for glucose in pancreatic α cells](https://npod.org/publication/g6pc2-controls-glucagon-secretion-by-defining-the-set-point-for-glucose-in-pancreatic-α-cells/) - Publication Categories: ORIGINAL Bahl V, Rifkind R, Waite E, Hamdan Z, May CL, Manduchi E, Voight BF, Lee MYY, Tigue M, Manuto N, Glaser B, Avrahami D, Kaestner KH. G6PC2 controls glucagon secretion by defining the set point for glucose in pancreatic α cells. Sci Transl Med. 2025 Jan;17(779):eadi6148. doi: 10.1126/scitranslmed.adi6148. Epub 2025 Jan 1.
- [Leveraging pre-trained machine learning models for islet quantification in type 1 diabetes](https://npod.org/publication/leveraging-pre-trained-machine-learning-models-for-islet-quantification-in-type-1-diabetes/) - Publication Categories: ORIGINAL Kang S, Penaloza Aponte JD, Elashkar O, Morales JF, Waddington N, Lamb DG, Ju H, Campbell-Thompson M, Kim S. Leveraging pre-trained machine learning models for islet quantification in type 1 diabetes. J Pathol Inform. 2024 Nov 8;16:100406. doi: 10.1016/j.jpi.2024.100406. PMID: 39720415; PMCID: PMC11665367.
- [Quantitative analysis of islet prohormone convertase 1/3 expression in human pancreas donors with diabetes](https://npod.org/publication/quantitative-analysis-of-islet-prohormone-convertase-1-3-expression-in-human-pancreas-donors-with-diabetes/) - Publication Categories: ORIGINAL Apaolaza PS, Chen YC, Grewal K, Lurz Y, Boulassel S, Verchere CB, Rodriguez-Calvo T. Quantitative analysis of islet prohormone convertase 1/3 expression in human pancreas donors with diabetes. Diabetologia. 2024 Oct 15. doi: 10.1007/s00125-024-06275-5. PMID: 39404844.
- [Combined assembloid modeling and 3D whole-organ mapping captures the microanatomy and function of the human fallopian tube](https://npod.org/publication/combined-assembloid-modeling-and-3d-whole-organ-mapping-captures-the-microanatomy-and-function-of-the-human-fallopian-tube/) - Publication Categories: ORIGINAL Crawford AJ, Forjaz A, Bons J, Bhorkar I, Roy T, Schell D, Queiroga V, Ren K, Kramer D, Huang W, Russo GC, Lee MH, Wu PH, Shih IM, Wang TL, Atkinson MA, Schilling B, Kiemen AL, Wirtz D. Combined assembloid modeling and 3D whole-organ mapping captures the microanatomy and function of the
- [Segmentation in large-scale cellular electron microscopy with deep learning: A literature survey](https://www.sciencedirect.com/science/article/pii/S1361841523001809?via=ihub#new_tab) - Publication Categories: REVIEW Aswath A, Alsahaf A, Giepmans BNG, Azzopardi G. Segmentation in large-scale cellular electron microscopy with deep learning: A literature survey. Med Image Anal. 2023 Aug 6;89:102920. Epub ahead of print. PMID: 37572414.
- [SliceChip: a benchtop fluidic platform for organotypic culture and serial assessment of human and rodent pancreatic slices](https://npod.org/publication/slicechip-a-benchtop-fluidic-platform-for-organotypic-culture-and-serial-assessment-of-human-and-rodent-pancreatic-slices/) - Publication Categories: ORIGINAL Alver CG, Álvarez-Cubela S, Altilio I, Hutchison E, Warrner E, Viso ME, Vitale G, Oliver D, Pastori RL, Dominguez-Bendala J, Agarwal A. SliceChip: a benchtop fluidic platform for organotypic culture and serial assessment of human and rodent pancreatic slices. Lab Chip. 2024 Jan 11. Epub ahead of print. PMID: 38205530.
- [Exocrine pancreas in type 1 and type 2 diabetes: different patterns of fibrosis, metaplasia, angiopathy, and adiposity](https://npod.org/publication/exocrine-pancreas-in-type-1-and-type-2-diabetes-different-patterns-of-fibrosis-metaplasia-angiopathy-and-adiposity/) - Publication Categories: ORIGINAL Wright JJ, Eskaros A, Windon A, Bottino R, Jenkins R, Bradley AM, Aramandla R, Philips S, Kang H, Saunders DC, Brissova M, Powers AC. Exocrine pancreas in type 1 and type 2 diabetes: different patterns of fibrosis, metaplasia, angiopathy, and adiposity. Diabetes. 2023 Oct 26:db230009. Epub ahead of print. PMID: 37881846.
- [Deletion of Carboxypeptidase E in β-Cells Disrupts Proinsulin Processing but Does Not Lead to Spontaneous Development of Diabetes in Mice](https://npod.org/publication/deletion-of-carboxypeptidase-e-in-β-cells-disrupts-proinsulin-processing-but-does-not-lead-to-spontaneous-development-of-diabetes-in-mice/) - Publication Categories: ORIGINAL Chen YC, Taylor AJ, Fulcher JM, Swensen AC, Dai XQ, Komba M, Wrightson KLC, Fok K, Patterson AE, Klein Geltink RI, MacDonald PE, Qian WJ, Verchere CB. Deletion of Carboxypeptidase E in β-Cells Disrupts Proinsulin Processing but Does Not Lead to Spontaneous Development of Diabetes in Mice. Diabetes. 2023 Sep 1;72(9):1277-1288. PMID:
- [Capillary contact points determine beta cell polarity, control secretion and are disrupted in the db/db mouse model of diabetes](https://npod.org/publication/capillary-contact-points-determine-beta-cell-polarity-control-secretion-and-are-disrupted-in-the-db-db-mouse-model-of-diabetes/) - Publication Categories: ORIGINAL Jevon D, Cottle L, Hallahan N, Harwood R, Samra JS, Gill AJ, Loudovaris T, Thomas HE, Thorn P. Capillary contact points determine beta cell polarity, control secretion and are disrupted in the db/db mouse model of diabetes. Diabetologia. 2024 May 30. doi: 10.1007/s00125-024-06180-x. Epub ahead of print. PMID: 38814445.
- [NKX2-2 based nuclei sorting on frozen human archival pancreas enables the enrichment of islet endocrine populations for single-nucleus RNA sequencing](https://npod.org/publication/nkx2-2-based-nuclei-sorting-on-frozen-human-archival-pancreas-enables-the-enrichment-of-islet-endocrine-populations-for-single-nucleus-rna-sequencing/) - Publication Categories: ORIGINAL Xie G, Toledo MP, Hu X, Yong HJ, Sanchez PS, Liu C, Naji A, Irianto J, Wang YJ. NKX2-2 based nuclei sorting on frozen human archival pancreas enables the enrichment of islet endocrine populations for single-nucleus RNA sequencing. BMC Genomics. 2024 Apr 30;25(1):427. doi: 10.1186/s12864-024-10335-w. PMID: 38689254.
- [Repositioning the Early Pathology of Type 1 Diabetes to the Extraislet Vasculature](https://npod.org/publication/repositioning-the-early-pathology-of-type-1-diabetes-to-the-extraislet-vasculature/) - Publication Categories: ORIGINAL Costanzo A, Clarke D, Holt M, Sharma S, Nagy K, Tan X, Kain L, Abe B, Luce S, Boitard C, Wyseure T, Mosnier LO, Su AI, Grimes C, Finn MG, Savage PB, Gottschalk M, Pettus J, Teyton L. Repositioning the Early Pathology of Type 1 Diabetes to the Extraislet Vasculature. J Immunol.
- [Redox regulation of m6A methyltransferase METTL3 in β-cells controls the innate immune response in type 1 diabetes](https://npod.org/publication/redox-regulation-of-m6a-methyltransferase-mettl3-in-β-cells-controls-the-innate-immune-response-in-type-1-diabetes/) - Publication Categories: ORIGINAL F De Jesus D, Zhang Z, Brown NK, Li X, Xiao L, Hu J, Gaffrey MJ, Fogarty G, Kahraman S, Wei J, Basile G, Rana TM, Mathews C, Powers AC, Parent AV, Atkinson MA, Dhe-Paganon S, Eizirik DL, Qian WJ, He C, Kulkarni RN. Redox regulation of m6A methyltransferase METTL3 in β-cells
- [CD4+ T cells from individuals with type 1 diabetes respond to a novel class of deamidated peptides formed in pancreatic islets](https://npod.org/publication/cd4-t-cells-from-individuals-with-type-1-diabetes-respond-to-a-novel-class-of-deamidated-peptides-formed-in-pancreatic-islets/) - Publication Categories: ORIGINAL Callebaut A, Guyer P, Derua R, Buitinga M, Manganaro A, Yi X, Sodré FMC, Vig S, Suleiman M, Marchetti P, Eizirik DL, Kent SC, Mathieu C, James EA, Overbergh L. CD4+ T cells from individuals with type 1 diabetes respond to a novel class of deamidated peptides formed in pancreatic islets. Diabetes.
- [DNA methylation-based assessment of cell composition in human pancreas and islets](https://npod.org/publication/dna-methylation-based-assessment-of-cell-composition-in-human-pancreas-and-islets/) - Publication Categories: ORIGINAL Drawshy Z, Neiman D, Fridlich O, Peretz A, Magenheim J, Rozo AV, Doliba NM, Stoffers DA, Kaestner KH, Schatz DA, Wasserfall C, Campbell-Thompson M, Shapiro J, Kaplan T, Shemer R, Glaser B, Klochendler A, Dor Y. DNA methylation-based assessment of cell composition in human pancreas and islets. Diabetes. 2024 Jan 24:db230704. Epub
- [TMEM219 regulates the transcription factor expression and proliferation of beta cells](https://npod.org/publication/tmem219-regulates-the-transcription-factor-expression-and-proliferation-of-beta-cells/) - Publication Categories: ORIGINAL D'Addio F, Assi E, Maestroni A, Rossi G, Usuelli V, Petrazzuolo A, Nardini M, Loretelli C, Ben Nasr M, Fiorina P. TMEM219 regulates the transcription factor expression and proliferation of beta cells. Front Endocrinol (Lausanne). 2024 Jan 22;15:1306127. doi: 10.3389/fendo.2024.1306127. PMID: 38318298; PMCID: PMC10839017.
- [Extensive elimination of acinar cells during normal postnatal pancreas growth](https://npod.org/publication/extensive-elimination-of-acinar-cells-during-normal-postnatal-pancreas-growth/) - Publication Categories: ORIGINAL Stolovich-Rain M, Fridlich O, Azulai S, Klochendler A, Anzi S, Magenheim J, Stein I, Mushasha F, Glaser B, Pikarsky E, Ben-Zvi D, Dor Y. Extensive elimination of acinar cells during normal postnatal pancreas growth. Cell Rep. 2023 Nov 22;42(12):113457. Epub ahead of print. PMID: 37995187.
- [Dynamic scRNA-seq of live human pancreatic slices reveals functional endocrine cell neogenesis through an intermediate ducto-acinar stage](https://npod.org/publication/dynamic-scrna-seq-of-live-human-pancreatic-slices-reveals-functional-endocrine-cell-neogenesis-through-an-intermediate-ducto-acinar-stage/) - Publication Categories: ORIGINAL Doke M, Álvarez-Cubela S, Klein D, Altilio I, Schulz J, Mateus Gonçalves L, Almaça J, Fraker CA, Pugliese A, Ricordi C, Qadir MMF, Pastori RL, Domínguez-Bendala J. Dynamic scRNA-seq of live human pancreatic slices reveals functional endocrine cell neogenesis through an intermediate ducto-acinar stage. Cell Metab. 2023 Oct 20:S1550-4131(23)00370-4. Epub ahead of
- [A bright future for glucagon and alpha cell biology](https://npod.org/publication/a-bright-future-for-glucagon-and-alpha-cell-biology/) - Publication Categories: REVIEW Panzer JK, Caicedo A. A bright future for glucagon and alpha cell biology. J Endocrinol. 2023 Oct 1:JOE-22-0315. Epub ahead of print. PMID: 37888975.
- [SARS-CoV-2 spread to endocrine organs is associated with obesity: an autopsy study of COVID-19 cases](https://npod.org/publication/sars-cov-2-spread-to-endocrine-organs-is-associated-with-obesity-an-autopsy-study-of-covid-19-cases/) - Publication Categories: ORIGINAL Poma AM, Basolo A, Alì G, Bonuccelli D, Di Stefano I, Conti M, Mazzetti P, Sparavelli R, Vignali P, Macerola E, Pistello M, Santini F, Basolo F, Toniolo A. SARS-CoV-2 spread to endocrine organs is associated with obesity: an autopsy study of COVID-19 cases. Endocrine. 2023 Sep 12. Epub ahead of print.
- [Pericyte dysfunction and impaired vasomotion are hallmarks of islets during the pathogenesis of type 1 diabetes](https://npod.org/publication/pericyte-dysfunction-and-impaired-vasomotion-are-hallmarks-of-islets-during-the-pathogenesis-of-type-1-diabetes/) - Publication Categories: ORIGINAL Mateus Gonçalves L, Fahd Qadir MM, Boulina M, Makhmutova M, Pereira E, Almaça J. Pericyte dysfunction and impaired vasomotion are hallmarks of islets during the pathogenesis of type 1 diabetes. Cell Rep. 2023 Aug 29;42(8):112913. j.celrep.2023.112913. Epub 2023 Aug 1. PMID: 37531253.
- [Species-specific roles for the MAFA and MAFB transcription factors in regulating islet β cell identity](https://npod.org/publication/species-specific-roles-for-the-mafa-and-mafb-transcription-factors-in-regulating-islet-β-cell-identity/) - Publication Categories: ORIGINAL Cha J, Tong X, Walker EM, Dahan T, Cochrane VA, Ashe S, Russell R, Osipovich AB, Mawla AM, Guo M, Liu JH, Loyd ZA, Huising MO, Magnuson MA, Hebrok M, Dor Y, Stein R. Species-specific roles for the MAFA and MAFB transcription factors in regulating islet β cell identity. JCI Insight. 2023
- [Islet cells in T1D: from recent advances to novel therapies - a symposium-based roadmap for future](https://joe.bioscientifica.com/view/journals/joe/aop/joe-23-0082/joe-23-0082.xml#new_tab) - Publication Categories: REVIEW Cantley J, Eizirik DL, Latres E, Dayan CM. Islet cells in T1D: from recent advances to novel therapies - a symposium-based roadmap for future. J Endocrinol. 2023 Jul 1:JOE-23-0082. Epub ahead of print. PMID: 37493471.
- [m6A mRNA methylation by METTL14 regulates early pancreatic cell differentiation](https://npod.org/publication/m6a-mrna-methylation-by-mettl14-regulates-early-pancreatic-cell-differentiation/) - Publication Categories: ORIGINAL Kahraman S, De Jesus DF, Wei J, Brown NK, Zou Z, Hu J, Pirouz M, Gregory RI, He C, Kulkarni RN. m6A mRNA methylation by METTL14 regulates early pancreatic cell differentiation. EMBO J. 2024 Sep 25. doi: 10.1038/s44318-024-00213-2. Epub ahead of print. PMID: 39322760.
- [mRNA Technology and Mucosal Immunization](https://npod.org/publication/mrna-technology-and-mucosal-immunization/) - Publication Categories: REVIEW Toniolo A, Maccari G, Camussi G. mRNA Technology and Mucosal Immunization. Vaccines (Basel). 2024 Jun 17;12(6):670. doi: 10.3390/vaccines12060670. PMID: 38932399; PMCID: PMC11209623.
- [A genomic data archive from the Network for Pancreatic Organ donors with Diabetes](https://www.nature.com/articles/s41597-023-02244-6#new_tab) - Publication Categories: ORIGINAL Perry DJ, Shapiro MR, Chamberlain SW, Kusmartseva I, Chamala S, Balzano-Nogueira L, Yang M, Brant JO, Brusko M, Williams MD, McGrail KM, McNichols J, Peters LD, Posgai AL, Kaddis JS, Mathews CE, Wasserfall CH, Webb-Robertson BM, Campbell-Thompson M, Schatz D, Evans-Molina C, Pugliese A, Concannon P, Anderson MS, German MS, Chamberlain CE,
- [The human α cell in health and disease](https://joe.bioscientifica.com/view/journals/joe/aop/joe-22-0298/joe-22-0298.xml#new_tab) - Publication Categories: REVIEW Pettway YD, Saunders DC, Brissova M. The human α cell in health and disease. J Endocrinol. 2023 Apr 1:JOE-22-0298. doi: 10.1530/JOE-22-0298. Epub ahead of print. PMID: 37114672.
- [Transcriptional Profile of Human Pancreatic Acinar Ductal Metaplasia](https://www.ghadvances.org/article/S2772-5723(23)00027-4/fulltext#new_tab) - Publication Categories: ORIGINAL Jinmai Jiang, Hesamedin Hakimjavadi, Julie K. Bray, Corey Perkins, Alyssa Gosling, Lais daSilva, Gamze Bulut, Jamel Ali, V. Wendy Setiawan, Martha Campbell-Thompson, Srikar Chamala, Thomas D. Schmittgen. Transcriptional Profile of Human Pancreatic Acinar Ductal Metaplasia. Gastro Hep Advances. 2023 Feb 7;2:532–543.
- [The extent and magnitude of islet T cell infiltration as powerful tools to define the progression to type 1 diabetes](https://link.springer.com/article/10.1007/s00125-023-05888-6#new_tab) - Publication Categories: ORIGINAL Apaolaza PS, Balcacean D, Zapardiel-Gonzalo J, Rodriguez-Calvo T. The extent and magnitude of islet T cell infiltration as powerful tools to define the progression to type 1 diabetes. Diabetologia. 2023 Mar 8. Epub ahead of print. PMID: 36884056.
- [DNA methylation Dependent Restriction of Tyrosine Hydroxylase Contributes to Pancreatic β-cell Heterogeneity](https://diabetesjournals.org/diabetes/article/doi/10.2337/db22-0506/148253/DNA-methylation-Dependent-Restriction-of-Tyrosine#new_tab) - Publication Categories: ORIGINAL Parveen N, Wang JK, Bhattacharya S, Cuala J, Singh Rajkumar M, Butler AE, Wu X, Shih HP, Georgia SK, Dhawan S. DNA methylation Dependent Restriction of Tyrosine Hydroxylase Contributes to Pancreatic β-cell Heterogeneity. Diabetes. 2023 Jan 6:db220506. Epub ahead of print. PMID: 36607262.
- [A discovery-based proteomics approach identifies protein disulphide isomerase (PDIA1) as a biomarker of β cell stress in type 1 diabetes](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9719098/#new_tab) - Publication Categories: ORIGINAL Syed F, Singhal D, Raedschelders K, Krishnan P, Bone RN, McLaughlin MR, Van Eyk JE, Mirmira RG, Yang ML, Mamula MJ, Wu H, Liu X, Evans-Molina C. A discovery-based proteomics approach identifies protein disulphide isomerase (PDIA1) as a biomarker of β cell stress in type 1 diabetes. EBioMedicine. 2022 Dec 1;87:104379. Epub
- [Restoring glutamate receptor signaling in pancreatic alpha cells rescues glucagon responses in type 1 diabetes](https://www.cell.com/cell-reports/fulltext/S2211-1247(22)01680-1?_returnURL=https://linkinghub.elsevier.com/retrieve/pii/S2211124722016801?showall=true#new_tab) - Publication Categories: ORIGINAL Panzer JK, Tamayo A, Caicedo A. Restoring glutamate receptor signaling in pancreatic alpha cells rescues glucagon responses in type 1 diabetes. Cell Rep. 2022 Dec 13;41(11):111792. PMID: 36516761.
- [Development of Type 1 Diabetes may occur through a Type 2 Diabetes mechanism](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9794996/#new_tab) - Publication Categories: ORIGINAL Josefsen K, Krogvold L, Gerling IC, Pociot F, Dahl-Jørgensen K, Buschard K. Development of Type 1 Diabetes may occur through a Type 2 Diabetes mechanism. Front Endocrinol (Lausanne). 2022 Dec 14;13:1032822. PMID: 36589856; PMCID: PMC9794996.
- [Abnormal Expression of an Insulin Synthesizing Enzyme in Islets of Adult Autoantibody Positive Donors](https://journals.sagepub.com/doi/10.1369/00221554221138368?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub  0pubmed#new_tab) - Publication Categories: ORIGINAL Teitelman G. Abnormal Expression of an Insulin Synthesizing Enzyme in Islets of Adult Autoantibody Positive Donors. J Histochem Cytochem. 2022 Oct;70(10):695-706. doi: 10.1369/00221554221138368. Epub 2022 Nov 5. PMID: 36341551.
- [PDE12 in type 1 diabetes](https://www.nature.com/articles/s41598-022-22890-x#new_tab) - Publication Categories: ORIGINAL Tekin H, Josefsen K, Krogvold L, Dahl-Jørgensen K, Gerling I, Pociot F, Buschard K. PDE12 in type 1 diabetes. Sci Rep. 2022 Oct 28;12(1):18149. PMID: 36307540; PMCID: PMC9614732.
- [Non-coding variants disrupting a tissue-specific regulatory element in HK1 cause congenital hyperinsulinism](https://www.nature.com/articles/s41588-022-01204-x#new_tab) - Publication Categories: ORIGINAL Wakeling MN, Owens NDL, Hopkinson JR, Johnson MB, Houghton JAL, Dastamani A, Flaxman CS, Wyatt RC, Hewat TI, Hopkins JJ, Laver TW, van Heugten R, Weedon MN, De Franco E, Patel KA, Ellard S, Morgan NG, Cheesman E, Banerjee I, Hattersley AT, Dunne MJ; International Congenital Hyperinsulinism Consortium, Richardson SJ, Flanagan SE.
- [Milder loss of insulin-containing islets in individuals with type 1 diabetes and type 2 diabetes-associated TCF7L2 genetic variants](https://link.springer.com/article/10.1007/s00125-022-05818-y#new_tab) - Publication Categories: ORIGINAL Redondo MJ, Richardson SJ, Perry D, Minard CG, Carr ALJ, Brusko T, Kusmartseva I, Pugliese A, Atkinson MA. Milder loss of insulin-containing islets in individuals with type 1 diabetes and type 2 diabetes-associated TCF7L2 genetic variants. Diabetologia. 2022 Oct 25. doi: 10.1007/s00125-022-05818-y. Epub ahead of print. PMID: 36282337.Redondo MJ, Richardson SJ, Perry
- [Images From the Exeter Archival Diabetes Biobank Now Accessible via Pancreatlas](https://diabetesjournals.org/care/article/doi/10.2337/dc22-1613/147618/Images-From-the-Exeter-Archival-Diabetes-Biobank#new_tab) - Publication Categories: Letter to the Editor Morgan NG, Richardson SJ, Powers AC, Saunders DC, Brissova M. Images From the Exeter Archival Diabetes Biobank Now Accessible via Pancreatlas. Diabetes Care. 2022 Oct 14:dc221613. Epub ahead of print. PMID: 36239401.
- [Understanding islet dysfunction in type 2 diabetes through multidimensional pancreatic phenotyping: The Human Pancreas Analysis Program](https://www.sciencedirect.com/science/article/pii/S155041312200403X?via=ihub) - Publication Categories: PERSPECTIVE Shapira SN, Naji A, Atkinson MA, Powers AC, Kaestner KH. Understanding islet dysfunction in type 2 diabetes through multidimensional pancreatic phenotyping: The Human Pancreas Analysis Program. Cell Metab. 2022 Sep 30:S1550-4131(22)00403-X. Epub ahead of print. PMID: 36206763.
- [Recognition of mRNA Splice Variant and Secretory Granule Epitopes by CD4+ T Cells in Type 1 Diabetes](https://europepmc.org/article/med/36201618) - Publication Categories: ORIGINAL Guyer P, Arribas-Layton D, Manganaro A, Speake C, Lord S, Eizirik DL, Kent SC, Mallone R, James EA. Recognition of mRNA Splice Variant and Secretory Granule Epitopes by CD4+ T Cells in Type 1 Diabetes. Diabetes. 2022 Oct 6:db220191. Epub ahead of print. PMID: 36201618.
- [3D chromatin maps of the human pancreas reveal lineage-specific regulatory architecture of T2D risk](https://www.sciencedirect.com/science/article/pii/S1550413122003576?via=ihubhttp://#new_tab) - Publication Categories: ORIGINAL Su C, Gao L, May CL, Pippin JA, Boehm K, Lee M, Liu C, Pahl MC, Golson ML, Naji A; HPAP Consortium, Grant SFA, Wells AD, Kaestner KH. 3D chromatin maps of the human pancreas reveal lineage-specific regulatory architecture of T2D risk. Cell Metab. 2022 Sep 6;34(9):1394-1409.e4. PMID: 36070683.
- [Live enteroviruses, but not other viruses, detected in human pancreas at the onset of type 1 diabetes in the DiViD study](https://link.springer.com/article/10.1007/s00125-022-05779-2#new_tab) - Publication Categories: ORIGINAL Krogvold L, Genoni A, Puggioni A, Campani D, Richardson SJ, Flaxman CS, Edwin B, Buanes T, Dahl-Jørgensen K, Toniolo A. Live enteroviruses, but not other viruses, detected in human pancreas at the onset of type 1 diabetes in the DiViD study. Diabetologia. 2022 Aug 12. Epub ahead of print. PMID: 35953727.
- [Adaptation to chronic ER stress enforces pancreatic β-cell plasticity](https://www.nature.com/articles/s41467-022-32425-7#new_tab) - Publication Categories: ORIGINAL Chen CW, Guan BJ, Alzahrani MR, Gao Z, Gao L, Bracey S, Wu J, Mbow CA, Jobava R, Haataja L, Zalavadia AH, Schaffer AE, Lee H, LaFramboise T, Bederman I, Arvan P, Mathews CE, Gerling IC, Kaestner KH, Tirosh B, Engin F, Hatzoglou M. Adaptation to chronic ER stress enforces pancreatic β-cell
- [High Prevalence of Common Human Viruses in Thyroid Tissue](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9333159/#new_tab) - Publication Categories: ORIGINAL Weider T, Genoni A, Broccolo F, Paulsen TH, Dahl-Jørgensen K, Toniolo A, Hammerstad SS. High Prevalence of Common Human Viruses in Thyroid Tissue. Front Endocrinol (Lausanne). 2022 Jul 14;13:938633. PMID: 35909527; PMCID: PMC9333159.
- [α Cell dysfunction in islets from nondiabetic, glutamic acid decarboxylase autoantibody-positive individuals](https://www.jci.org/articles/view/156243#new_tab) - Publication Categories: ORIGINAL Doliba NM, Rozo AV, Roman J, Qin W, Traum D, Gao L, Liu J, Manduchi E, Liu C, Golson ML, Vahedi G, Naji A, Matschinsky FM, Atkinson MA, Powers AC, Brissova M, Kaestner KH, Stoffers DA; HPAP Consortium. α Cell dysfunction in islets from nondiabetic, glutamic acid decarboxylase autoantibody-positive individuals. J Clin
- [Microscopic modulation and analysis of islets of Langerhans in living zebrafish larvae](https://febs.onlinelibrary.wiley.com/doi/epdf/10.1002/1873-3468.14411#new_tab) - Publication Categories: ORIGINAL Faraj N, Duinkerken BHP, Carroll EC, Giepmans BNG. Microscopic modulation and analysis of islets of Langerhans in living zebrafish larvae. FEBS Lett. 2022 May 29. Epub ahead of print. PMID: 35644832.
- [The Chd4 subunit of the NuRD complex regulates Pdx1-controlled genes involved in β-cell function](https://jme.bioscientifica.com/view/journals/jme/aop/jme-22-0011/jme-22-0011.xml#new_tab) - Publication Categories: ORIGINAL Davidson RK, Weaver SA, Casey N, Kanojia S, Hogarth E, Schneider Aguirre R, Sims EK, Evans-Molina C, Spaeth JM. The Chd4 subunit of the NuRD complex regulates Pdx1-controlled genes involved in β-cell function. J Mol Endocrinol. 2022 May 1:JME-22-0011. Epub ahead of print. PMID: 35521759.
- [Pancreas Whole Tissue Transcriptomics Highlights the Role of the Exocrine Pancreas in Patients With Recently Diagnosed Type 1 Diabetes](https://www.frontiersin.org/articles/10.3389/fendo.2022.861985/full#new_tab) - Publication Categories: ORIGINAL Välikangas T, Lietzén N, Jaakkola MK, Krogvold L, Eike MC, Kallionpää H, Tuomela S, Mathews C, Gerling IC, Oikarinen S, Hyöty H, Dahl-Jorgensen K, Elo LL, Lahesmaa R. Pancreas Whole Tissue Transcriptomics Highlights the Role of the Exocrine Pancreas in Patients With Recently Diagnosed Type 1 Diabetes. Front Endocrinol (Lausanne). 2022 Apr
- [Generation of human islet cell type-specific identity genesets](https://www.nature.com/articles/s41467-022-29588-8#new_tab) - Publication Categories: ORIGINAL van Gurp L, Fodoulian L, Oropeza D, Furuyama K, Bru-Tari E, Vu AN, Kaddis JS, Rodríguez I, Thorel F, Herrera PL. Generation of human islet cell type-specific identity genesets. Nat Commun. 2022 Apr 19;13(1):2020. PMID: 35440614.
- [Increased Expression of Viral Sensor MDA5 in Pancreatic Islets and in Hormone-Negative Endocrine Cells in Recent Onset Type 1 Diabetic Donors](https://www.frontiersin.org/articles/10.3389/fimmu.2022.833141/full#new_tab) - Publication Categories: ORIGINAL Nigi L, Brusco N, Grieco GE, Fignani D, Licata G, Formichi C, Aiello E, Marselli L, Marchetti P, Krogvold L, Jorgensen KD, Sebastiani G, Dotta F. Increased Expression of Viral Sensor MDA5 in Pancreatic Islets and in Hormone-Negative Endocrine Cells in Recent Onset Type 1 Diabetic Donors. Front Immunol. 2022 Mar 11;13:833141.
- [Exocrine Pancreatic Enzymes Are a Serological Biomarker for Type 1 Diabetes Staging and Pancreas Size](https://diabetesjournals.org/diabetes/article-abstract/70/4/944/39277/Exocrine-Pancreatic-Enzymes-Are-a-Serological?redirectedFrom=fulltext#new_tab) - Publication Categories: ORIGINAL Ross JJ, Wasserfall CH, Bacher R, Perry DJ, McGrail K, Posgai AL, Dong X, Muir A, Li X, Campbell-Thompson M, Brusko TM, Schatz DA, Haller MJ, Atkinson MA. Exocrine Pancreatic Enzymes Are a Serological Biomarker for Type 1 Diabetes Staging and Pancreas Size. Diabetes. 2021 Apr;70(4):944-954. Epub 2021 Jan 13. PMID: 33441381;
- [Adipose tissue in COVID-19: detection of SARS-CoV-2 in adipocytes and activation of the interferon-alpha response](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8852916/#new_tab) - Publication Categories: ORIGINAL Basolo A, Poma AM, Bonuccelli D, et al. Adipose tissue in COVID-19: detection of SARS-CoV-2 in adipocytes and activation of the interferon-alpha response [published online ahead of print, 2022 Feb 15]. J Endocrinol Invest. 2022;1-9.
- [Upregulation of HLA class II in pancreatic beta cells from organ donors with type 1 diabetes](https://link.springer.com/article/10.1007/s00125-021-05619-9#new_tab) - Publication Categories: ORIGINAL Quesada-Masachs E, Zilberman S, Rajendran S, Chu T, McArdle S, Kiosses WB, Lee JM, Yesildag B, Benkahla MA, Pawlowska A, Graef M, Pfeiffer S, Mikulski Z, von Herrath M. Upregulation of HLA class II in pancreatic beta cells from organ donors with type 1 diabetes. Diabetologia. 2022 Feb;65(2):387-401. Epub 2021 Dec 21.
- [Substance Use Affects Type 1 Diabetes Pancreas Pathology: Implications for Future Studies](https://www.frontiersin.org/articles/10.3389/fendo.2021.778912/full#new_tab) - Publication Categories: ORIGINAL Bruggeman BS, Campbell-Thompson M, Filipp SL, Gurka MJ, Atkinson MA, Schatz DA, Jacobsen LM. Substance Use Affects Type 1 Diabetes Pancreas Pathology: Implications for Future Studies. Front Endocrinol (Lausanne). 2021 Nov 29;12:778912. PMID: 34912300; PMCID: PMC8667172.
- [The IGFBP3/TMEM219 pathway regulates beta cell homeostasis](https://www.nature.com/articles/s41467-022-28360-2#new_tab) - Publication Categories: ORIGINAL D'Addio F, Maestroni A, Assi E, Ben Nasr M, Amabile G, Usuelli V, Loretelli C, Bertuzzi F, Antonioli B, Cardarelli F, El Essawy B, Solini A, Gerling IC, Bianchi C, Becchi G, Mazzucchelli S, Corradi D, Fadini GP, Foschi D, Markmann JF, Orsi E, Škrha J Jr, Camboni MG, Abdi R, James
- [The Impact of Pro-Inflammatory Cytokines on Alternative Splicing Patterns in Human Islets](https://npod.org/publication/the-impact-of-pro-inflammatory-cytokines-on-alternative-splicing-patterns-in-human-islets/) - Publication Categories: ORIGINAL Wu W, Syed F, Simpson E, Lee CC, Liu J, Chang G, Dong C, Seitz C, Eizirik DL, Mirmira RG, Liu Y, Evans-Molina C. The Impact of Pro-Inflammatory Cytokines on Alternative Splicing Patterns in Human Islets. Diabetes. 2022;71(1):116–127. PMID: 34697029.
- [Heterogeneity of human anti-viral immunity shaped by virus, tissue, age, and sex](https://www.sciencedirect.com/science/article/pii/S2211124721015576#new_tab) - Publication Categories: ORIGINAL Poon MML, Byington E, Meng W, Kubota M, Matsumoto R, Grifoni A, Weiskopf D, Dogra P, Lam N, Szabo PA, Ural BB, Wells SB, Rosenfeld AM, Brusko MA, Brusko TM, Connors TJ, Sette A, Sims PA, Luning Prak ET, Shen Y, Farber DL. Heterogeneity of human anti-viral immunity shaped by virus, tissue,
- [Enteroviruses and Type 1 Diabetes: Multiple Mechanisms and Factors?](https://www.annualreviews.org/doi/pdf/10.1146/annurev-med-042320-015952#new_tab) - Publication Categories: REVIEW Lloyd RE, Tamhankar M, Lernmark Å. Enteroviruses and Type 1 Diabetes: Multiple Mechanisms and Factors? Annual review of medicine. 2021 Oct 4;73:30. PMID:34794324.
- [Pancreas Pathology in Type 1 Diabetes](https://joe.bioscientifica.com/view/journals/joe/aop/joe-21-0358/joe-21-0358.xml#new_tab) - Publication Categories: REVIEW Richardson SJ, Pugliese A. Pancreas Pathology in Type 1 Diabetes: An evolving Story. J Endocrinol. 2021 Nov 1:JOE-21-0358. Epub ahead of print. PMID: 34755679.
- [Diabetes: Concepts of β-Cell Organ Dysfunction and Failure Would Lead to Earlier Diagnoses and Prevention](https://diabetes.diabetesjournals.org/content/70/11/2444#new_tab) - Publication Categories: PERSPECTIVE Charles MA, Leslie RD. Diabetes: Concepts of β-Cell Organ Dysfunction and Failure Would Lead to Earlier Diagnoses and Prevention. Diabetes. 2021 Nov;70(11):2444-2456. PMID: 34711669.
- [SARS-CoV-2 infection generates tissue-localized immunological memory in humans](https://www.science.org/doi/10.1126/sciimmunol.abl9105#new_tab) - Publication Categories: ORIGINAL Poon MML, Rybkina K, Kato Y, Kubota M, Matsumoto R, Bloom NI, Zhang Z, Hastie KM, Grifoni A, Weiskopf D, Wells SB, Ural BB, Lam N, Szabo PA, Dogra P, Lee YS, Gray JI, Bradley MC, Brusko MA, Brusko TM, Saphire EO, Connors TJ, Sette A, Crotty S, Farber DL. SARS-CoV-2 infection
- [Tet2 Controls the Responses of β cells to Inflammation in Autoimmune Diabetes](https://npod.org/publication/tet2-controls-the-responses-of-β-cells-to-inflammation-in-autoimmune-diabetes/) - Publication Categories: ORIGINAL Rui J, Deng S, Perdigoto AL, Ponath G, Kursawe R, Lawlor N, Sumida T, Levine-Ritterman M, Stitzel ML, Pitt D, Lu J, Herold KC. Tet2 Controls the Responses of β cells to Inflammation in Autoimmune Diabetes. Nat Commun. 2021 Aug 20;12(1):5074. PMID: 34417463.
- [Human islet T cells are highly reactive to preproinsulin in type 1 diabetes](https://www.pnas.org/content/118/41/e2107208118#new_tab) - Publication Categories: ORIGINAL Anderson AM, Landry LG, Alkanani AA, Pyle L, Powers AC, Atkinson MA, Mathews CE, Roep BO, Michels AW, Nakayama M. Human islet T cells are highly reactive to preproinsulin in type 1 diabetes. Proc Natl Acad Sci U S A. 2021 Oct 12;118(41):e2107208118. PMID: 34611019.
- [The Immunoregulatory Role of the Signal Regulatory Protein Family and CD47 Signaling Pathway in Type 1 Diabetes](https://www.frontiersin.org/articles/10.3389/fimmu.2021.739048/full#new_tab) - Publication Categories: REVIEW Sharp RC, Brown ME, Shapiro MR, Posgai AL, Brusko TM. The Immunoregulatory Role of the Signal Regulatory Protein Family and CD47 Signaling Pathway in Type 1 Diabetes. Front Immunol. 2021 Sep 16;12:739048. PMID: 34603322; PMCID: PMC8481641.
- [Localization of Enteroviral RNA within the Pancreas in Donors with T1D and T1D-associated Autoantibodies](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385321/#new_tab) - Publication Categories: ORIGINAL Geravandi S, Richardson S, Pugliese A, Maedler K. Localization of enteroviral RNA within the pancreas in donors with T1D and T1D-associated autoantibodies. Cell Rep Med. 2021 Aug 17;2(8):100371. PMID: 34467248; PMCID: PMC8385321.
- [MERTK on Mononuclear Phagocytes Regulates T Cell Antigen Recognition at Autoimmune and Tumor Sites](https://npod.org/publication/mertk-on-mononuclear-phagocytes-regulates-t-cell-antigen-recognition-at-autoimmune-and-tumor-sites/) - Publication Categories: ORIGINAL Lindsay RS, Whitesell JC, Dew KE, Rodriguez E, Sandor AM, Tracy D, Yannacone SF, Basta BN, Jacobelli J, Friedman RS. MERTK on mononuclear phagocytes regulates T cell antigen recognition at autoimmune and tumor sites. J Exp Med. 2021 Oct 4;218(10):e20200464. Epub 2021 Aug 20. PMID: 34415994.
- [Defining a Cure for Type 1 Diabetes: A Call to Action](https://npod.org/publication/defining-a-cure-for-type-1-diabetes-a-call-to-action/) - Publication Categories: COMMENT Roep BO, Montero E, van Tienhoven R, Atkinson MA, Schatz DA, Mathieu C. Defining a cure for type 1 diabetes: a call to action. Lancet Diabetes Endocrinol. 2021 Jul 30:S2213-8587(21)00181-9. Epub ahead of print. PMID: 34339632.
- [ACE2 Chromogenic Immunostaining Protocol Optimized for Formalin-fixed Paraffin-embedded Human Tissue Sections](https://npod.org/publication/ace2-chromogenic-immunostaining-protocol-optimized-for-formalin-fixed-paraffin-embedded-human-tissue-sections/) - Publication Categories: ORIGINAL Jorgensen M, Joseph P, Posgai AL, Vander Heide RS, Kusmartseva I, Atkinson MA. ACE2 chromogenic immunostaining protocol optimized for formalin-fixed paraffin-embedded human tissue sections. STAR Protoc. 2021 Sep 17;2(3):100696. Epub 2021 Jul 21. PMID: 34308375; PMCID: PMC8292114.
- [Enhancing African American Participation in Biospecimens: A Case in Point for Pancreatic Cancer. Cancer Health Disparities](https://npod.org/publication/enhancing-african-american-participation-in-biospecimens-a-case-in-point-for-pancreatic-cancer-cancer-health-disparities/) - Publication Categories: ORIGINAL Behar-Horenstein L, Warren RC, Setiawan VW, Perkins C, Schmittgen TD. Enhancing African American Participation in Biospecimens: A Case in Point for Pancreatic Cancer. Cancer Health Disparities. 2020 Dec;29(12):10.1158/1538-7755.DISP20-PO-236. PMID: 34296063; PMCID: PMC8294622.
- [Pancreatic Ppy-expressing γ-cells Display Mixed Phenotypic Traits and the Adaptive Plasticity to Engage Insulin Production](https://npod.org/publication/pancreatic-ppy-expressing-γ-cells-display-mixed-phenotypic-traits-and-the-adaptive-plasticity-to-engage-insulin-production/) - Publication Categories: ORIGINAL Perez-Frances M, van Gurp L, Abate MV, Cigliola V, Furuyama K, Bru-Tari E, Oropeza D, Carreaux T, Fujitani Y, Thorel F, Herrera PL. Pancreatic Ppy-expressing γ-cells display mixed phenotypic traits and the adaptive plasticity to engage insulin production. Nat Commun. 2021 Jul 22;12(1):4458. PMID: 34294685.
- [Breaking and Restoring Immune Tolerance to Pancreatic Beta-cells in Type 1 Diabetes](https://journals.lww.com/co-endocrinology/Fulltext/2021/08000/Breaking_and_restoring_immune_tolerance_to.11.aspx#new_tab) - Publication Categories: ORIGINAL Erdem N, Montero E, Roep BO. Breaking and restoring immune tolerance to pancreatic beta-cells in type 1 diabetes. Curr Opin Endocrinol Diabetes Obes. 2021 Aug 1;28(4):397-403. PMID: 34183540.
- [Altered Cellular Localisation and Expression, Together with Unconventional Protein Trafficking, of Prion Protein, PrPC, in Type 1 Diabetes](https://link.springer.com/article/10.1007/s00125-021-05501-8#new_tab) - Publication Categories: ORIGINAL Hiller H, Yang C, Beachy DE, Kusmartseva I, Candelario-Jalil E, Posgai AL, Nick HS, Schatz D, Atkinson MA, Wasserfall CH. Altered cellular localisation and expression, together with unconventional protein trafficking, of prion protein, PrPC, in type 1 diabetes. Diabetologia. 2021 Jul 17. Epub ahead of print. PMID: 34274990.
- [Pancreas Optical Clearing and 3-D Microscopy in Health and Diabetes](https://www.frontiersin.org/articles/10.3389/fendo.2021.644826/full#new_tab) - Publication Categories: REVIEW Campbell-Thompson M, Tang SC. Pancreas Optical Clearing and 3-D Microscopy in Health and Diabetes. Front Endocrinol (Lausanne). 2021 Apr 26;12:644826. PMID: 33981285; PMCID: PMC8108133.
- [Pancreatic Histopathology of Human Monogenic Diabetes Due to Causal Variants in KCNJ11, HNF1A, GATA6, and LMNA](https://academic.oup.com/jcem/article/103/1/35/4082869#new_tab) - Publication Categories: ORIGINAL Sanyoura M, Jacobsen L, Carmody D, Del Gaudio D, Alkorta-Aranburu G, Arndt K, Hu Y, Kobiernicki F, Kusmartseva I, Atkinson MA, Philipson LH, Schatz D, Campbell-Thompson M, Greeley SAW. Pancreatic Histopathology of Human Monogenic Diabetes Due to Causal Variants in KCNJ11, HNF1A, GATA6, and LMNA. J Clin Endocrinol Metab. 2018 Jan 1;103(1):35-45.
- [Means, Motive, and Opportunity: Do Non-Islet-Reactive Infiltrating T Cells Contribute to Autoimmunity in Type 1 Diabetes?](https://www.frontiersin.org/articles/10.3389/fimmu.2021.683091/full#new_tab) - Publication Categories: REVIEW Rodriguez-Calvo T, Christoffersson G, Bender C, von Herrath MG, Mallone R, Kent SC, James EA. Means, Motive, and Opportunity: Do Non-Islet-Reactive Infiltrating T Cells Contribute to Autoimmunity in Type 1 Diabetes? Front Immunol. 2021 Jun 16;12:683091. PMID: 34220832; PMCID: PMC8242234.
- [Revisiting the role of inflammation in the loss of pancreatic β-cells in T1DM](https://www.nature.com/articles/s41574-020-00409-6#new_tab) - Publication Categories: COMMENT Eizirik DL, Colli ML. Revisiting the role of inflammation in the loss of pancreatic β-cells in T1DM [published online ahead of print, 2020 Aug 28]. Nat Rev Endocrinol. 2020;10.1038/s41574-020-00409-6.
- [There is More than One Way to Reach Type 2 Diabetes](https://www.nature.com/articles/s42255-021-00415-6#new_tab) - Publication Categories: REVIEW Gloyn AL, Powers AC. There is more than one way to reach type 2 diabetes. Nat Metab. 2021 Jun 28. doi: 10.1038/s42255-021-00415-6. Epub ahead of print. PMID: 34183851.
- [Delayed Diagnosis of Diabetic Ketoacidosis and Associated Mortality During the COVID-19 Pandemic](https://onlinelibrary.wiley.com/doi/10.1111/1753-0407.13208#new_tab) - Publication Categories: ORIGINAL Foster TP, Haller MJ, Atkinson MA, Schatz DA. Delayed Diagnosis of Diabetic Ketoacidosis and Associated Mortality During the COVID-19 Pandemic. J Diabetes. 2021 Jun 30. Epub ahead of print. PMID: 34196115.
- [Monogenic Diabetes and Integrated Stress Response Genes Display Altered Gene Expression in Type 1 Diabetes](https://diabetes.diabetesjournals.org/content/early/2021/05/20/db21-0070#new_tab) - Publication Categories: ORIGINAL Hiller H, Beachy DE, Lebowitz JJ, Engler S, Mason JR, Miller DR, Kusmarteva I, Jacobsen LM, Posgai AL, Khoshbouei H, Oram RA, Schatz DA, Hattersley AT, Bodenmiller B, Atkinson MA, Nick HS, Wasserfall CH. Monogenic Diabetes and Integrated Stress Response Genes Display Altered Gene Expression in Type 1 Diabetes. Diabetes. 2021 May
- [Whole-Slide Image Analysis of Human Pancreas Samples to Elucidate the Immunopathogenesis of Type 1 Diabetes Using the QuPath Software](https://www.frontiersin.org/articles/10.3389/fmolb.2021.689799/full#new_tab) - Publication Categories: ORIGINAL Apaolaza PS, Petropoulou PI, Rodriguez-Calvo T. Whole-Slide Image Analysis of Human Pancreas Samples to Elucidate the Immunopathogenesis of Type 1 Diabetes Using the QuPath Software. Front Mol Biosci. 2021 Jun 11;8:689799. PMID: 34179094; PMCID: PMC8226255.
- [Detection of Enterovirus in the Islet Cells of Patients with Type 1 Diabetes: What Do We Learn from Immunohistochemistry?](https://link.springer.com/article/10.1007/s00125-013-3138-z#new_tab) - Publication Categories: Letter to the Editor Hansson SF, Korsgren S, Pontén F, Korsgren O. Detection of enterovirus in the islet cells of patients with type 1 diabetes: what do we learn from immunohistochemistry? Diabetologia. 2014 Mar;57(3):645-6. Epub 2013 Dec 19. PMID: 24352376.
- [Genetic Deficiency or Pharmacological Inhibition of Soluble Epoxide Hydrolase Ameliorates High Fat Diet-Induced Pancreatic β-Cell Dysfunction and Loss](https://npod.org/publication/genetic-deficiency-or-pharmacological-inhibition-of-soluble-epoxide-hydrolase-ameliorates-high-fat-diet-induced-pancreatic-β-cell-dysfunction-and-loss/) - Publication Categories: ORIGINAL Koike S, Hsu MF, Bettaieb A, Chu B, Matsumoto N, Morisseau C, Havel PJ, Huising MO, Hammock BD, Haj FG. Genetic deficiency or pharmacological inhibition of soluble epoxide hydrolase ameliorates high fat diet-induced pancreatic β-cell dysfunction and loss. Free Radic Biol Med. 2021 May 23:S0891-5849(21)00321-X. Epub ahead of print. PMID: 34038767.
- [The Long and Winding Road to Understanding and Conquering Type 1 Diabetes](https://www.sciencedirect.com/science/article/pii/S1074761310001287?via=ihub#new_tab) - Publication Categories: PERSPECTIVE Santamaria P. The long and winding road to understanding and conquering type 1 diabetes. Immunity. 2010 Apr 23;32(4):437-45. PMID: 20412754.
- [Global Gene Expression Changes in Type 1 Diabetes: Insights into Autoimmune Response in the Target Organ and in the Periphery](https://www.sciencedirect.com/science/article/pii/S0165247810001951?via=ihub#new_tab) - Publication Categories: ORIGINAL Planas R, Pujol-Borrell R, Vives-Pi M. Global gene expression changes in type 1 diabetes: insights into autoimmune response in the target organ and in the periphery. Immunol Lett. 2010 Oct 30;133(2):55-61. Epub 2010 Aug 11. PMID: 20708640.
- [Metagenomics and Personalized Medicine](https://www.sciencedirect.com/science/article/pii/S0092867411010634?via=ihub#new_tab) - Publication Categories: PERSPECTIVE Virgin HW, Todd JA. Metagenomics and personalized medicine. Cell. 2011 Sep 30;147(1):44-56. PMID: 21962506; PMCID: PMC4618802.
- [Juvenile Diabetes](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3461727/#new_tab) - Publication Categories: ORIGINAL Babu SR, Eisenbarth GS. Juvenile diabetes. Indian J Med Res. 2012 Aug;136(2):179-81. PMID: 22960882; PMCID: PMC3461727.
- [Islet-Derived CD4 T Cells Targeting Proinsulin in Human Autoimmune Diabetes](https://diabetes.diabetesjournals.org/content/66/3/722#new_tab) - Publication Categories: ORIGINAL Michels AW, Landry LG, McDaniel KA, Yu L, Campbell-Thompson M, Kwok WW, Jones KL, Gottlieb PA, Kappler JW, Tang Q, Roep BO, Atkinson MA, Mathews CE, Nakayama M. Islet-Derived CD4 T Cells Targeting Proinsulin in Human Autoimmune Diabetes. Diabetes. 2017 Mar;66(3):722-734. Epub 2016 Dec 5. PMID: 27920090; PMCID: PMC5319719.
- [The Polycomb-Dependent Epigenome Controls β Cell Dysfunction, Dedifferentiation, and Diabetes](https://www.sciencedirect.com/science/article/pii/S1550413118302560?via=ihub#new_tab) - Publication Categories: ORIGINAL Lu TT, Heyne S, Dror E, Casas E, Leonhardt L, Boenke T, Yang CH, Sagar, Arrigoni L, Dalgaard K, Teperino R, Enders L, Selvaraj M, Ruf M, Raja SJ, Xie H, Boenisch U, Orkin SH, Lynn FC, Hoffman BG, Grün D, Vavouri T, Lempradl AM, Pospisilik JA. The Polycomb-Dependent Epigenome Controls β
- [Ectonucleoside Triphosphate Diphosphohydrolase-3 Antibody Targets Adult Human Pancreatic β Cells for In Vitro and In Vivo Analysis](https://www.sciencedirect.com/science/article/pii/S1550413118306399?via=ihubhttp://#new_tab) - Publication Categories: ORIGINAL Saunders DC, Brissova M, Phillips N, Shrestha S, Walker JT, Aramandla R, Poffenberger G, Flaherty DK, Weller KP, Pelletier J, Cooper T, Goff MT, Virostko J, Shostak A, Dean ED, Greiner DL, Shultz LD, Prasad N, Levy SE, Carnahan RH, Dai C, Sévigny J, Powers AC. Ectonucleoside Triphosphate Diphosphohydrolase-3 Antibody Targets Adult
- [Cellular Stressors May Alter Islet Hormone Cell Proportions by Moderation of Alternative Splicing Patterns](https://academic.oup.com/hmg/article/28/16/2763/5487670#new_tab) - Publication Categories: ORIGINAL Jeffery N, Richardson S, Chambers D, Morgan NG, Harries LW. Cellular stressors may alter islet hormone cell proportions by moderation of alternative splicing patterns. Hum Mol Genet. 2019 Aug 15;28(16):2763-2774. PMID: 31098640; PMCID: PMC6687954.
- [Does Rotavirus Turn on Type 1 Diabetes?](https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1007965#new_tab) - Publication Categories: ORIGINAL Harrison LC, Perrett KP, Jachno K, Nolan TM, Honeyman MC. Does rotavirus turn on type 1 diabetes? PLoS Pathog. 2019 Oct 10;15(10):e1007965.1007965. PMID: 31600345; PMCID: PMC6786515.
- [Type 1 Diabetes Mellitus as a Disease of the β-Cell (Do Not Blame the Immune System?)](https://www.nature.com/articles/s41574-020-00443-4#new_tab) - Publication Categories: REVIEW Roep BO, Thomaidou S, van Tienhoven R, Zaldumbide A. Type 1 diabetes mellitus as a disease of the β-cell (do not blame the immune system?). Nat Rev Endocrinol. 2021 Mar;17(3):150-161. Epub 2020 Dec 8. PMID: 33293704; PMCID: PMC7722981.
- [Type 1 Diabetes Mellitus: Much Progress, Many Opportunities](https://www.jci.org/articles/view/142242#new_tab) - Publication Categories: REVIEW Powers AC. Type 1 diabetes mellitus: much progress, many opportunities. J Clin Invest. 2021 Apr 15;131(8):142242. PMID: 33759815.
- [The Human Islet: Mini-organ with Mega-impact](https://academic.oup.com/edrv/advance-article/doi/10.1210/endrev/bnab010/6223906#new_tab) - Publication Categories: REVIEW Walker JT, Saunders DC, Brissova M, Powers AC. The Human Islet: Mini-organ with Mega-impact. Endocr Rev. 2021. Epub 2021 Apr 12. PMID: 33844836.
- [In Vitro Generation of Peri-Islet Basement Membrane-Like Structures](https://www.sciencedirect.com/science/article/pii/S0142961221001642?via=ihub#new_tab) - Publication Categories: ORIGINAL Santini-González J, Simonovich JA, Castro-Gutiérrez R, González-Vargas Y, Abuid NJ, Stabler CL, Russ HA, Phelps EA. In vitro generation of peri-islet basement membrane-like structures. Biomaterials. 2021 Jun;273:120808. Epub 2021 Apr 13. PMID: 33895491; PMCID: PMC8131001.
- [Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices](https://www.jove.com/t/62207/observing-islet-function-islet-immune-cell-interactions-live#new_tab) - Publication Categories: ORIGINAL Huber MK, Drotar DM, Hiller H, Beery ML, Joseph P, Kusmartseva I, Speier S, Atkinson MA, Mathews CE, Phelps EA. Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices. J Vis Exp. 2021 Apr 12;(170). PMID: 33900291.
- [Proinsulin-Reactive CD4 T Cells in the Islets of Type 1 Diabetes Organ Donors](https://www.frontiersin.org/articles/10.3389/fendo.2021.622647/full#new_tab) - Publication Categories: ORIGINAL Landry LG, Anderson AM, Russ HA, Yu L, Kent SC, Atkinson MA, Mathews CE, Michels AW, Nakayama M. Proinsulin-Reactive CD4 T Cells in the Islets of Type 1 Diabetes Organ Donors. Front Endocrinol (Lausanne). 2021 Mar 25;12:622647. PMID: 33841327; PMCID: PMC8027116.
- [Islet Sympathetic Innervation and Islet Neuropathology in Patients with Type 1 Diabetes](https://www.nature.com/articles/s41598-021-85659-8#new_tab) - Publication Categories: ORIGINAL Campbell-Thompson M, Butterworth EA, Boatwright JL, Nair MA, Nasif LH, Nasif K, Revell AY, Riva A, Mathews CE, Gerling IC, Schatz DA, Atkinson MA. Islet sympathetic innervation and islet neuropathology in patients with type 1 diabetes. Sci Rep. 2021 Mar 22;11(1):6562. PMID: 33753784; PMCID: PMC7985489.
- [Expression of Immunoreactive Inducible Nitric Oxide Dynthase in Pancreatic Islet Cells from Newly Diagnosed and Long-Term Type 1 Diabetic Donors is Heterogeneous and Not Disease-Associated](https://link.springer.com/article/10.1007/s00441-020-03340-4#new_tab) - Publication Categories: ORIGINAL Reddy S, Krogvold L, Martin C, Sun KX, Martin O, Al-Ani A, Dahl-Jørgensen K. Expression of immunoreactive inducible nitric oxide synthase in pancreatic islet cells from newly diagnosed and long-term type 1 diabetic donors is heterogeneous and not disease-associated. Cell Tissue Res. Epub 2021 Jan 11. PMID: 33427953.
- [Structural and Functional Polarisation of Human Pancreatic Beta Cells in Islets from Organ Donors with and without Type 2 Diabetes](https://link.springer.com/article/10.1007/s00125-020-05345-8#new_tab) - Publication Categories: ORIGINAL Cottle L, Gan WJ, Gilroy I, Samra JS, Gill AJ, Loudovaris T, Thomas HE, Hawthorne WJ, Kebede MA, Thorn P. Structural and functional polarisation of human pancreatic beta cells in islets from organ donors with and without type 2 diabetes. Diabetologia. 2021 Mar;64(3):618-629. Epub 2021 Jan 5. PMID: 33399909; PMCID: PMC7864831.
- [Immunomodulatory Dual-Sized Microparticle System Conditions Human Antigen Presenting Cells Into a Tolerogenic Phenotype In Vitro and Inhibits Type 1 Diabetes-Specific Autoreactive T Cell Responses](https://www.frontiersin.org/articles/10.3389/fimmu.2020.574447/full#new_tab) - Publication Categories: ORIGINAL Brusko MA, Stewart JM, Posgai AL, Wasserfall CH, Atkinson MA, Brusko TM, Keselowsky BG. Immunomodulatory Dual-Sized Microparticle System Conditions Human Antigen Presenting Cells Into a Tolerogenic Phenotype In Vitro and Inhibits Type 1 Diabetes-Specific Autoreactive T Cell Responses. Front Immunol. 2020 Oct 22;11:574447. PMID: 33193362; PMCID: PMC7649824.
- [Intracrine Testosterone Activation in Human Pancreatic β Cells Stimulates Insulin Secretion](https://diabetes.diabetesjournals.org/content/69/11/2392#new_tab) - Publication Categories: ORIGINAL Xu W, Schiffer L, Qadir MMF, Zhang Y, Hawley J, Mota De Sa P, Keevil BG, Wu H, Arlt W, Mauvais-Jarvis F. Intracrine Testosterone Activation in Human Pancreatic β-Cells Stimulates Insulin Secretion. Diabetes. 2020 Nov;69(11):2392-2399. Epub 2020 Aug 27. PMID: 32855171; PMCID: PMC7576567.
- [Interpreting Type 1 Diabetes Risk with Genetics and Single-Cell Epigenomics](https://npod.org/publication/interpreting-type-1-diabetes-risk-with-genetics-and-single-cell-epigenomics/) - Publication Categories: ORIGINAL Chiou J, Geusz RJ, Okino ML, Han JY, Miller M, Melton R, Beebe E, Benaglio P, Huang S, Korgaonkar K, Heller S, Kleger A, Preissl S, Gorkin DU, Sander M, Gaulton KJ. Interpreting type 1 diabetes risk with genetics and single-cell epigenomics. Nature. 2021 May. Epub 2021 May 19. PMID: 34012112.
- [Optical Imaging of Pancreatic Innervation](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112238/#new_tab) - Publication Categories: ORIGINAL Makhmutova M, Caicedo A. Optical Imaging of Pancreatic Innervation. Front Endocrinol (Lausanne). 2021 Apr 27;12:663022. PMID:33986728.
- [Insulitis Revisited](https://npod.org/publication/insulitis-revisited/) - Publication Categories: REVIEW Roep B (2016). Insulitis Revisited. Diabetes. 2016 Mar; 65(3):545-7.
- [P16Ink4a - induced senescence of pancreatic beta cells enhances insulin secretion](https://npod.org/publication/p16ink4a-induced-senescence-of-pancreatic-beta-cells-enhances-insulin-secretion/) - Publication Categories: ORIGINAL Helman A, Klochendler A, Azazmeh N, Gabai Y, Horwtiz E, Anzi S, Swisa A, Condiotti R, Granit R, Nevo Y, Fixler Y, Shreibmen D, Zamir A, Tornovsky-Babeay S, Dai C, Glaser B, Powers A, Shapiro AM, Magnuson M, Dor Y, Ben-Porath I (2016). P16Ink4a -induced senescence of pancreatic beta cells enhances insulin secretion.
- [Analysis of Self-antigen Specificity of Islet-infiltrating T Cells from Human Donors with Type 1 Diabetes](https://npod.org/publication/analysis-of-self-antigen-specificity-of-islet-infiltrating-t-cells-from-human-donors-with-type-1-diabetes/) - Publication Categories: ORIGINAL Babon JA, DeNicola ME, Blodgett DM, Crèvecoeur I, Buttrick TS, Maehr R, Bottino R, Naji A, Kaddis J, Elyaman W, James EA, Haliyur R, Brissova M, Overbergh L, Mathieu C, Delong T, Haskins K, Pugliese A, Campbell-Thompson M, Matthews C, Atkinson M, Powers AC, Harlan DM, Kent SC (2016). Analysis of self-antigen
- [Re-Addressing the 2013 Consensus Guidelines for the Diagnosis of Insulitis in Human Type 1 Diabetes: Is Change Necessary?](https://npod.org/publication/re-addressing-the-2013-consensus-guidelines-for-the-diagnosis-of-insulitis-in-human-type-1-diabetes-is-change-necessary/) - Publication Categories: REVIEW Campbell-Thompson M, Atkinson M, Butler A, Giepmans B, von Herrath M, Hyöty H, Kay T, Morgan N, Powers A, Pugliese A, Richardson S, In't Veld P (2017). Re-Addressing the 2013 Consensus Guidelines for the Diagnosis of Insulitis in Human Type 1 Diabetes: Is Change Necessary? Diabetologia. 2017 Jan 9.
- [Increase in Pancreatic Proinsulin and Preservation of Beta Cell Mass in Autoantibody Positive Donors prior to Type 1 Diabetes Onset](https://npod.org/publication/increase-in-pancreatic-proinsulin-and-preservation-of-beta-cell-mass-in-autoantibody-positive-donors-prior-to-type-1-diabetes-onset/) - Publication Categories: ORIGINAL Rodriguez-Calvo R, Zapardiel-Gonzalo J, Amirian N, Castillo E, Lajevardi Y, Krogvold L, Dahl-Jørgensen K, von Herrath M (2017). Increase in Pancreatic Proinsulin and Preservation of Beta Cell Mass in Autoantibody Positive Donors prior to Type 1 Diabetes Onset. Diabetes. 2017 Jan 30.
- [α Cell Function and Gene Expression Are Compromised in Type 1 Diabetes](https://npod.org/publication/α-cell-function-and-gene-expression-are-compromised-in-type-1-diabetes/) - Publication Categories: ORIGINAL Brissova M, Haliyur R, Saunders D, Shrestha S, Dai C, Blodgett DM, Bottino R, Campbell-Thompson M, Aramandla R, Poffenberger G, Lindner J, Pan FC, von Herrath MG, Greiner DL, Shultz LD, Sanyoura M, Philipson LH, Atkinson M, Harlan DM, Levy SE, Prasad N, Stein R, Powers AC (2018). α Cell Function and Gene
- [Distribution of IL-1β immunoreactive cells in pancreatic biopsies from living volunteers with new-onset type 1 diabetes: comparison with donors without diabetes and with longer duration of disease.](https://npod.org/publication/distribution-of-il-1β-immunoreactive-cells-in-pancreatic-biopsies-from-living-volunteers-with-new-onset-type-1-diabetes-comparison-with-donors-without-diabetes-and-with-longer-duration-of-diseas/) - Publication Categories: ORIGINAL Reddy S, Krogvold L, Martin C, Holland R, Choi J, Woo H, Wu F, Dahl-Jørgensen K. (2018) Distribution of IL-1β immunoreactive cells in pancreatic biopsies from living volunteers with new-onset type 1 diabetes: comparison with donors without diabetes and with longer duration of disease. Diabetologia. 2018 Jun;61(6):1362-1373.
- [Association of Type 2 Diabetes with Submicron Titanium Dioxide Crystals in the Pancreas](https://npod.org/publication/association-of-type-2-diabetes-with-submicron-titanium-dioxide-crystals-in-the-pancreas/) - Publication Categories: ORIGINAL Heller A, Jarvis K, Coffman SS (2018). Association of Type 2 Diabetes with Submicron Titanium Dioxide Crystals in the Pancreas. Chem Res Toxicol. 2018 Jun 18;31(6):506-509.
- [Enteroviral Infections as a Trigger for Type 1 Diabetes](https://npod.org/publication/enteroviral-infections-as-a-trigger-for-type-1-diabetes/) - Publication Categories: REVIEW Rodriguez-Calvo T. (2018) Enteroviral Infections as a Trigger for Type 1 Diabetes. Curr Diab Rep. 2018 Sep 19;18(11):106.
- [Abnormal Neutrophil Signature in the Blood and Pancreas of Presymptomatic and Symptomatic Type 1 Diabetes](https://pubmed.ncbi.nlm.nih.gov/30232284/#new_tab) - Publication Categories: ORIGINAL Vecchio F, Lo Buono N, Stabilini A, Nigi L, Dufort MJ, Geyer S, Rancoita PM, Cugnata F, Mandelli A, Valle A, Leete P, Mancarella F, Linsley PS, Krogvold L, Herold KC, Elding Larsson H, Richardson SJ, Morgan NG, Dahl-Jørgensen K, Sebastiani G, Dotta F, Bosi E; DRI_Biorepository Group; Type 1 Diabetes TrialNet
- [Heterogeneous Expression of Proinsulin Processing Enzymes in Beta Cells of Non-diabetic and Type 2 Diabetic Humans](https://journals.sagepub.com/doi/10.1369/0022155419831641?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub++0pubmed&#new_tab) - Publication Categories: ORIGINAL Teitelman G. Heterogeneous Expression of Proinsulin Processing Enzymes in Beta Cells of Non-diabetic and Type 2 Diabetic Humans. J Histochem Cytochem. 2019;67(6):385-400.
- [HLA Class II Antigen Processing and Presentation Pathway Components Demonstrated by Transcriptome and Protein Analyses of islet β-Cells from Donors with Type 1 Diabetes.](https://npod.org/publication/hla-class-ii-antigen-processing-and-presentation-pathway-components-demonstrated-by-transcriptome-and-proteinanalyses-of-islet-β-cells-from-donors-with-type-1-diabetes/) - Publication Categories: ORIGINAL Russell MA, Redick SD, Blodgett DM, Richardson SJ, Leete P, Krogvold L, Dahl-Jørgensen K, Bottino R, Brissova M, Spaeth JM, Babon JAB, Haliyur R, Powers AC, Yang C, Kent SC, Derr AG, Kucukural A, Garber MG, Morgan NG, Harlan DM. (2019) HLA Class II Antigen Processing and Presentation Pathway Components Demonstrated by Transcriptome and ProteinAnalyses of islet β-Cells from Donors with Type 1 Diabetes. Diabetes. 2019 Mar 4.
- [SARS-CoV-2 Receptor Angiotensin I-Converting Enzyme Type 2 (ACE2) Is Expressed in Human Pancreatic β-Cells and in the Human Pancreas Microvasculature](https://www.frontiersin.org/articles/10.3389/fendo.2020.596898/full#new_tab) - Publication Categories: ORIGINAL Fignani D, Licata G, Brusco N, Nigi L, Grieco GE, Marselli L, Overbergh L, Gysemans C, Colli ML, Marchetti P, Mathieu C, Eizirik DL, Sebastiani G, Dotta F. SARS-CoV-2 Receptor Angiotensin I-Converting Enzyme Type 2 (ACE2) Is Expressed in Human Pancreatic β-Cells and in the Human Pancreas Microvasculature. Front Endocrinol (Lausanne). 2020 Nov
- [Islet Expression of Type I Interferon Response Sensors is Associated with Immune Infiltration and Viral Infection in Type 1 Diabetes](https://advances.sciencemag.org/content/7/9/eabd6527#new_tab) - Publication Categories: ORIGINAL Apaolaza PS, Balcacean D, Zapardiel-Gonzalo J, Nelson G, Lenchik N, Akhbari P, Gerling I, Richardson SJ, Rodriguez-Calvo T; nPOD-Virus Group. Islet expression of type I interferon response sensors is associated with immune infiltration and viral infection in type 1 diabetes. Sci Adv. 2021 Feb 24;7(9):eabd6527. PMID: 33627420; PMCID: PMC7904254.
- [HLA Class I Hyper-Expression Unmasks Beta Cells but not Alpha Cells to the Immune System in Pre-Diabetes](https://www.sciencedirect.com/science/article/pii/S0896841121000366?via=ihub#new_tab) - Publication Categories: ORIGINAL Benkahla MA, Sabouri S, Kiosses WB, Rajendran S, Quesada-Masachs E, von Herrath MG. HLA class I hyper-expression unmasks beta cells but not alpha cells to the immune system in pre-diabetes. J Autoimmun. 2021 May;119:102628. Epub 2021 Mar 9. PMID: 33706238.
- [Diabetes, Obesity, Metabolism, and SARS-CoV-2 Infection: The End of the Beginning](https://www.sciencedirect.com/science/article/pii/S1550413121000164?via=ihub#new_tab) - Publication Categories: REVIEW Drucker DJ. Diabetes, obesity, metabolism, and SARS-CoV-2 infection: the end of the beginning. Cell Metab. 2021 Mar 2;33(3):479-498. Epub 2021 Jan 21.PMID: 33529600.
- [Expression of SARS-CoV-2 Entry Factors in the Pancreas of Normal Organ Donors and Individuals with COVID-19](https://www.sciencedirect.com/science/article/pii/S1550413120306008?via=ihub#new_tab) - Publication Categories: ORIGINAL Kusmartseva I, Wu W, Syed F, et al. Expression of SARS-CoV-2 Entry Factors in the Pancreas of Normal Organ Donors and Individuals with COVID-19. Cell Metab. 2020;32(6):1041-1051.e6.
- [SARS-CoV-2 Cell Entry Factors ACE2 and TMPRSS2 Are Expressed in the Microvasculature and Ducts of Human Pancreas but Are Not Enriched in β Cells](https://www.sciencedirect.com/science/article/pii/S155041312030601X?via=ihub#new_tab) - Publication Categories: ORIGINAL Coate KC, Cha J, Shrestha S, Wang W, Gonçalves LM, Almaça J, Kapp ME, Fasolino M, Morgan A, Dai C, Saunders DC, Bottino R, Aramandla R, Jenkins R, Stein R, Kaestner KH, Vahedi G; HPAP Consortium, Brissova M, Powers AC. SARS-CoV-2 Cell Entry Factors ACE2 and TMPRSS2 Are Expressed in the Microvasculature and
- [Human Pluripotent Stem Cell-Based Organoids and Cell Platforms for Modelling SARS-CoV-2 Infection and Drug Discovery](https://www.sciencedirect.com/science/article/pii/S1873506121000532?via=ihub#new_tab) - Publication Categories: PERSPECTIVE Giani AM, Chen S. Human pluripotent stem cell-based organoids and cell platforms for modelling SARS-CoV-2 infection and drug discovery. Stem Cell Res. 2021 May;53:102207. Epub 2021 Feb 20. PMID: 33677394.
- [Gene Expression Analysis of the Pre-Diabetic Pancreas to Identify Pathogenic Mechanisms and Biomarkers of Type 1 Diabetes](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793767/#new_tab) - Publication Categories: ORIGINAL Yip L, Fuhlbrigge R, Alkhataybeh R, Fathman CG. Gene Expression Analysis of the Pre-Diabetic Pancreas to Identify Pathogenic Mechanisms and Biomarkers of Type 1 Diabetes. Front Endocrinol (Lausanne). 2020 Dec 23;11:609271. eCollection 2020. PMID: 33424774.
- [New Insights Into the Role of Autoreactive CD8 T Cells and Cytokines in Human Type 1 Diabetes](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813992/#new_tab) - Publication Categories: REVIEW Bender C, Rajendran S, von Herrath MG. New Insights Into the Role of Autoreactive CD8 T Cells and Cytokines in Human Type 1 Diabetes. Front Endocrinol (Lausanne). 2021 Jan 5;11:606434. eCollection 2020. PMID: 33469446.
- [Pancreatic Beta Cell Autophagy is Impaired in Type 1 Diabetes](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940272/#new_tab) - Publication Categories: ORIGINAL Muralidharan C, Conteh AM, Marasco MR, Crowder JJ, Kuipers J, de Boer P, Linnemann AK. Pancreatic beta cell autophagy is impaired in type 1 diabetes. Diabetologia. 2021 Apr;64(4):865-877. Epub 2021 Jan 30. PMID: 33515072; PMCID: PMC7940272.
- [Genetic Predisposition in the 2'-5'A Pathway in the Development of Type 1 Diabetes: Potential Contribution to Dysregulation of Innate Antiviral Immunity](https://link.springer.com/article/10.1007/s00125-021-05469-5#new_tab) - Publication Categories: ORIGINAL Pedersen K, Haupt-Jorgensen M, Krogvold L, Kaur S, Gerling IC, Pociot F, Dahl-Jørgensen K, Buschard K. Genetic predisposition in the 2'-5'A pathway in the development of type 1 diabetes: potential contribution to dysregulation of innate antiviral immunity. [published online ahead of print, 2021 May 11]. Diabetologia. 2021;S00125-021-05469-5. PMID: 33973017.
- [Immune Transcriptome of Cells Infected with Enterovirus Strains Obtained from Cases of Autoimmune Thyroid Disease](https://www.mdpi.com/2076-2607/9/4/876#new_tab) - Publication Categories: ORIGINAL Poma AM, Hammerstad SS, Genoni A, Basolo A, Dahl-Jorgensen K, Toniolo A. Immune Transcriptome of Cells Infected with Enterovirus Strains Obtained from Cases of Autoimmune Thyroid Disease. Microorganisms. 2021 Apr 19;9(4):876. PMID: 33921891; PMCID: PMC8073039.
- [Altered β-Cell Prohormone Processing and Secretion in Type 1 Diabetes](https://diabetes.diabetesjournals.org/content/early/2021/05/04/dbi20-0034#new_tab) - Publication Categories: PERSPECTIVE Rodriguez-Calvo T, Chen YC, Verchere CB, Haataja L, Arvan P, Leete P, Richardson SJ, Morgan NG, Qian WJ, Pugliese A, Atkinson M, Evans-Molina C, Sims EK. Altered β-Cell Prohormone Processing and Secretion in Type 1 Diabetes. [published online ahead of print, 2021 May]. Diabetes. 2021;dbi200034. PMID: 33947721.
- [Neoepitopes in Type 1 Diabetes: Etiological Insights, Biomarkers and Therapeutic Targets](https://www.frontiersin.org/articles/10.3389/fimmu.2021.667989/full#new_tab) - Publication Categories: PERSPECTIVE Rodriguez-Calvo T, Johnson JD, Overbergh L, Dunne JL. Neoepitopes in Type 1 Diabetes: Etiological Insights, Biomarkers and Therapeutic Targets. Front Immunol. 2021 Apr 19;12:667989. PMID: 33953728; PMCID: PMC8089389.
- [Identification and Characterisation of Tertiary Lymphoid Organs in Human Type 1 Diabetes](https://doi.org/10.1007/s00125-021-05453-z#new_tab) - Publication Categories: ORIGINAL Korpos É, Kadri N, Loismann S, Findeisen CR, Arfuso F, Burke GW 3rd, Richardson SJ, Morgan NG, Bogdani M, Pugliese A, Sorokin L. Identification and characterisation of tertiary lymphoid organs in human type 1 diabetes. [published online ahead of print, 2021 Apr 29]. Diabetologia. 2021;S00125-021-05453-z.
- [Human Duct Cells Contribute to β Cell Compensation in Insulin Resistance](https://doi.org/10.1172/jci.insight.99576#new_tab) - Publication Categories: ORIGINAL Dirice E, De Jesus DF, Kahraman S, Basile G, Ng RW, El Ouaamari A, Teo AKK, Bhatt S, Hu J, Kulkarni RN. Human Duct Cells Contribute to β Cell Compensation in Insulin Resistance. JCI Insight. 2019 Apr 18;4(8):e99576.
- [Pancreatlas: Applying an Adaptable Framework to Map the Human Pancreas in Health and Disease](https://www.sciencedirect.com/science/article/pii/S2666389920301616?via=ihub#new_tab) - Publication Categories: ORIGINAL Saunders DC, Messmer J, Kusmartseva I, et al. Pancreatlas: Applying an Adaptable Framework to Map the Human Pancreas in Health and Disease. Patterns (N Y). 2020;1(8):100120. Published 2020 Oct 5.
- [Image-Based Machine Learning Algorithms for Disease Characterization in the Human Type 1 Diabetes Pancreas](https://doi.org/10.1016/j.ajpath.2020.11.010#new_tab) - Publication Categories: ORIGINAL Tang X, Kusmartseva I, Kulkarni S, et al. Image-based machine learning algorithms for disease characterization in the human type 1 diabetes pancreas [published online ahead of print, 2020 Dec 8]. Am J Pathol. 2020;S0002-9440(20)30551-4.
- [Pancreatic Tissue Proteomics Unveils Key Proteins, Pathways, and Networks Associated with Type 1 Diabetes](https://onlinelibrary.wiley.com/doi/10.1002/prca.202000053#new_tab) - Publication Categories: ORIGINAL
- [Presumption of guilt for T cells in type 1 diabetes: lead culprits or partners in crime depending on age of onset?](https://link.springer.com/article/10.1007/s00125-020-05298-y#new_tab) - Publication Categories: REVIEW
- [The healthy exocrine pancreas contains preproinsulin-specific CD8 T cells that attack islets in type 1 diabetes](https://advances.sciencemag.org/content/6/42/eabc5586.full#new_tab) - Publication Categories: ORIGINAL
- [Beta cell dysfunction in diabetes: the islet microenvironment as an unusual suspect](https://link.springer.com/article/10.1007/s00125-020-05186-5#new_tab) - Publication Categories: REVIEW
- [Human Islets Contain a Beta Cell Type That Expresses Proinsulin But Not the Enzyme That Converts the Precursor to Insulin](https://journals.sagepub.com/doi/10.1369/0022155420961361#new_tab) - Publication Categories: ORIGINAL
- [Islet-reactive CD8+ T-cell frequencies in the pancreas but not blood distinguish type 1 diabetes from healthy donors](https://immunology.sciencemag.org/content/3/20/eaao4013.long#new_tab) - Publication Categories: ORIGINAL Culina S, Lalanne AI, Afonso G, et al. Islet-reactive CD8+ T cell frequencies in the pancreas, but not in blood, distinguish type 1 diabetic patients from healthy donors. Sci Immunol. 2018;3(20):eaao4013.
- [Lipid Droplet Accumulation in Human Pancreatic Islets Is Dependent On Both Donor Age and Health](https://diabetes.diabetesjournals.org/content/69/3/342.long#new_tab) - Publication Categories: ORIGINAL
- [Human herpesvirus-6 is present at higher levels in the pancreatic tissues of donors with type 1 diabetes](https://www.sciencedirect.com/science/article/pii/S0896841119305165?via=ihub#new_tab) - Publication Categories: ORIGINAL
- [IL-4R is expressed on alpha and beta cells of human pancreata](https://www.sciencedirect.com/science/article/pii/S1521661620301881?via=ihub) - Publication Categories: Letter to the Editor Rajendran S, Graef M, Chu T, von Herrath M. IL-4R is expressed on alpha and beta cells of human pancreata. Clin Immunol. 2020 May;214:108394 . Epub 2020 Mar 26. PMID: 32224156.
- [Organisation of the human pancreas in health and in diabetes](https://doi.org/10.1007/s00125-020-05203-7#new_tab) - Publication Categories: REVIEW Atkinson MA, Campbell-Thompson M, Kusmartseva I, Kaestner KH. Organisation of the human pancreas in health and in diabetes. Diabetologia. 2020 Oct;63(10):1966-1973. Epub 2020 Sep 7. PMID: 32894306.
- [Type 1 Diabetes: Interferons and the Aftermath of Pancreatic Beta-Cell Enteroviral Infection](https://doi.org/10.3390/microorganisms8091419#new_tab) - Publication Categories: REVIEW
- [Histological validation of a type 1 diabetes clinical diagnostic model for classification of diabetes](https://onlinelibrary.wiley.com/doi/full/10.1111/dme.14361#new_tab) - Publication Categories: ORIGINAL Carr ALJ, Perry DJ, Lynam AL, et al. Histological validation of a type 1 diabetes clinical diagnostic model for classification of diabetes [published online ahead of print, 2020 Jul 7]. Diabet Med. 2020;10.1111/dme.14361.
- [Large-scale electron microscopy database for human type 1 diabetes](https://pubmed.ncbi.nlm.nih.gov/32424134/?from_single_result=Large-scale+electron+microscopy+database+for+human+type+1+diabetes#new_tab) - Publication Categories: ORIGINAL de Boer P, Pirozzi NM, Wolters AHG, et al. Large-scale electron microscopy database for human type 1 diabetes. Nat Commun. 2020;11(1):2475. Published 2020 May 18.
- [Long-term culture of human pancreatic slices as a model to study real-time islet regeneration](https://www.nature.com/articles/s41467-020-17040-8#new_tab) - Publication Categories: ORIGINAL Qadir MMF, Álvarez-Cubela S, Weitz J, et al. Long-term culture of human pancreatic slices as a model to study real-time islet regeneration. Nat Commun. 2020;11(1):3265. Published 2020 Jun 29.
- [Immune Transcriptome of Cells Infected with Enterovirus Strains Obtained from Cases of Type 1 Diabetes](https://www.mdpi.com/2076-2607/8/7/1031/htm#new_tab) - Publication Categories: ORIGINAL Poma AM, Genoni A, Broccolo F, et al. Immune Transcriptome of Cells Infected with Enterovirus Strains Obtained from Cases of Type 1 Diabetes. Microorganisms. 2020;8(7):E1031. Published 2020 Jul 12.
- [Removing Formaldehyde-Induced Peptidyl Crosslinks Enables Mass Spectrometry Imaging of Peptide Hormone Distributions from Formalin-Fixed Paraffin-Embedded Tissues](https://doi.org/10.1002/anie.202008847#new_tab) - Publication Categories: ORIGINAL Lee DK, Rubakhin SS, Kusmartseva I, Wasserfall C, Atkinson MA, Sweedler JV. Removing Formaldehyde-Induced Peptidyl Crosslinks Enables Mass Spectrometry Imaging of Peptide Hormone Distributions from Formalin-Fixed Paraffin-Embedded Tissues [published online ahead of print, 2020 Aug 6]. Angew Chem Int Ed Engl. 2020;10.1002/anie.202008847
- [Rationale for enteroviral vaccination and antiviral therapies in human type 1 diabetes](https://doi.org/10.1007/s00125-019-4811-7#new_tab) - Publication Categories: REVIEW
- [Activation of the HIF1α/PFKFB3 stress response pathway in beta cells in type 1 diabetes](https://link.springer.com/article/10.1007/s00125-019-05030-5#new_tab) - Publication Categories: ORIGINAL Nomoto H, Pei L, Montemurro C, et al. Activation of the HIF1α/PFKFB3 stress response pathway in beta cells in type 1 diabetes. Diabetologia. 2020;63(1):149-161. doi:10.1007/s00125-019-05030-5
- [Abnormalities in proinsulin processing in islets from individuals with longstanding T1D](https://npod.org/publication/abnormalities-in-proinsulin-processing-in-islets-from-individuals-with-longstanding-t1d/) - Publication Categories: ORIGINAL Sims EK, Syed F, Nyalwidhe J, et al. Abnormalities in proinsulin processing in islets from individuals with longstanding T1D. Transl Res. 2019;213:90-99. doi:10.1016/j.trsl.2019.08.001
- [Single-cell Resolution Analysis of the Human Pancreatic Ductal Progenitor Cell Niche](https://npod.org/publication/single-cell-resolution-analysis-of-the-human-pancreatic-ductal-progenitor-cell-niche/) - Publication Categories: ORIGINAL
- [Enteroviral Infections in the Pathogenesis of Type 1 Diabetes: new insights for therapeutic intervention](https://npod.org/publication/enteroviral-infections-in-the-pathogenesis-of-type-1-diabetes-new-insights-for-therapeutic-intervention/) - Publication Categories: REVIEW Richardson SJ, Morgan NG. (2018) Enteroviral infections in the pathogenesis of type 1 diabetes: new insights for therapeutic intervention. Curr Opin Pharmacol. 2018 Jul 28;43:11-19.
- [MicroRNAs: markers of β-cell stress and autoimmunity](https://npod.org/publication/micrornas-markers-of-β-cell-stress-and-autoimmunity/) - Publication Categories: REVIEW Dotta F, Ventriglia G, Snowhite IV, Pugliese A. (2018). MicroRNAs: markers of β-cell stress and autoimmunity Curr Opin Endocrinol Diabetes Obes. 2018 Aug;25(4):237-245.
- [Fifty Years of Pancreatic Islet Pathology in Human Type 1 Diabetes: insights gained and progress made](https://npod.org/publication/fifty-years-of-pancreatic-islet-pathology-in-human-type-1-diabetes-insights-gained-and-progress-made/) - Publication Categories: REVIEW Morgan NG, Richardson SJ. (2018) Fifty years of pancreatic islet pathology in human type 1 diabetes: insights gained and progress made. Diabetologia. 2018 Sep 25.
- [The Role of Accessory Cells in Islet Homeostasis](https://npod.org/publication/the-role-of-accessory-cells-in-islet-homeostasis/) - Publication Categories: REVIEW Tang SC, Jessup CF, Campbell-Thompson M. (2018) The Role of Accessory Cells in Islet Homeostasis. Curr Diab Rep. 2018 Sep 28;18(11):117.
- [Pancreas Pathology During the Natural History of Type 1 Diabetes](https://npod.org/publication/pancreas-pathology-during-the-natural-history-of-type-1-diabetes-2/) - Publication Categories: REVIEW Rodriguez-Calvo T, Richardson SJ, Pugliese A. (2018) Pancreas Pathology During the Natural History of Type 1 Diabetes. Curr Diab Rep. 2018 Oct 6;18(11):124.
- [Determining Antigen Specificity of Human Islet Infiltrating T Cells in Type 1 Diabetes](https://npod.org/publication/determining-antigen-specificity-of-human-islet-infiltrating-t-cells-in-type-1-diabetes/) - Publication Categories: REVIEW Nakayama M, Michels AW. (2019) Determining Antigen Specificity of Human Islet Infiltrating T Cells in Type 1 Diabetes. Front Immunol. 2019 Mar 8;10:365.
- [Why do Current Protocols Not Halt the Underlying Disease Process?](https://npod.org/publication/why-do-current-protocols-not-halt-the-underlying-disease-process/) - Publication Categories: REVIEW Kolb H, von Herrath M (2017). Immunotherapy for Type 1 Diabetes: Why do Current Protocols Not Halt the Underlying Disease Process? Cell Metabolism. 2017 Feb 7; 25(2):233-241.
- [The Elusive Role of B Lymphocytes and Islet Autoantibodies in (Human) Type 1 Diabetes](https://npod.org/publication/the-elusive-role-of-b-lymphocytes-and-islet-autoantibodies-in-human-type-1-diabetes/) - Publication Categories: REVIEW Bloem SJ, Roep BO (2017). The Elusive Role of B Lymphocytes and Islet Autoantibodies in (Human) Type 1 Diabetes. Diabetologia. 2017 Jul;60(7):1185-1189.
- [Narrowing in on the Anti-β Cell-specific T Cells: Looking 'Where the Action Is'](https://npod.org/publication/narrowing-in-on-the-anti-β-cell-specific-t-cells-looking-where-the-action-is/) - Publication Categories: REVIEW Kent S, Babon J (2017). Narrowing in on the Anti-β Cell-specific T Cells: Looking 'Where the Action Is'. Curr Opin Endocrinol Diabetes Obes. 2017 Apr; 24(2):98-102.
- [Hyaluronan Deposition in Islets May Precede and Direct the Location of Islet Immune-Cell Infiltrates](https://npod.org/publication/hyaluronan-deposition-in-islets-may-precede-and-direct-the-location-of-islet-immune-cell-infiltrates/) - Publication Categories: ORIGINAL
- [Obesity-Dependent Accumulation of Titanium in the Pancreas of Type 2 Diabetic Donors](https://npod.org/publication/obesity-dependent-accumulation-of-titanium-in-the-pancreas-of-type-2-diabetic-donors/) - Publication Categories: ORIGINAL Heller A, Coffman SS, Friedman KA. Obesity-Dependent Accumulation of Titanium in the Pancreas of Type 2 Diabetic Donors. Chem Res Toxicol. 2019;32(7):1351‐1356.
- [Pancreas tissue slices from organ donors enable in situ analysis of type 1 diabetes pathogenesis](https://npod.org/publication/pancreas-tissue-slices-from-organ-donors-enable-in-situ-analysis-of-type-1-diabetes-pathogenesis/) - Publication Categories: ORIGINAL Panzer JK, Hiller H, Cohrs CM, Almaça J, Enos SJ, Beery M, Cechin S, Drotar DM, Weitz JR, Santini J, Huber MK, Muhammad Fahd Qadir M, Pastori RL, Domínguez-Bendala J, Phelps EA, Atkinson MA, Pugliese A, Caicedo A, Kusmartseva I, Speier S. 2020. Pancreas tissue slices from organ donors enable in situ analysis of type 1 diabetes pathogenesis. JCI Insight. 2020 Apr 23;5(8).
- [Hypusinated eIF5A is Expressed in the Pancreas and Spleen of Individuals with Type 1 and Type 2 Diabetes](https://npod.org/publication/hypusinated-eif5a-is-expressed-in-the-pancreas-and-spleen-of-individuals-with-type-1-and-type-2-diabetes/) - Publication Categories: ORIGINAL Mastracci TL, Colvin SC, Padgett LR, Mirmira RG (2020) Hypusinated eIF5A is expressed in the pancreas and spleen of individuals with type 1 and type 2 diabetes. PLoS ONE 15(3): e0230627.
- [Temporal Analysis of Amylase Expression in Control, Autoantibody-Positive, and Type 1 Diabetes Pancreatic Tissues](https://npod.org/publication/temporal-analysis-of-amylase-expression-in-control-autoantibody-positive-and-type-1-diabetes-pancreatic-tissues/) - Publication Categories: ORIGINAL Kusmartseva I, Beery M, Hiller H, Padilla M, Selman S, Posgai A, Nick HS, Campbell-Thompson M, Schatz DA, Haller MJ, Wasserfall CH, Atkinson MA. (2019) Temporal Analysis of Amylase Expression in Control, Autoantibody-Positive, and Type 1 Diabetes Pancreatic Tissues. Diabetes. 2020 Jan;69(1):60-66.
- [A Map of Human Type 1 Diabetes Progression by Imaging Mass Cytometry](https://npod.org/publication/a-map-of-human-type-1-diabetes-progression-by-imaging-mass-cytometry-2/) - Publication Categories: ORIGINAL Damond N, Engler S, Zanotelli VRT, Schapiro D, Wasserfall CH, Kusmartseva I, Nick HS, Thorel F, Herrera PL, Atkinson MA, Bodenmiller B. (2020) A Map of Human Type 1 Diabetes Progression by Imaging Mass Cytometry. Cell Metab. 2019 Mar 5;29(3):755-768.
- [Islet Amyloidosis in a Child with Type 1 Diabetes](https://npod.org/publication/islet-amyloidosis-in-a-child-with-type-1-diabetes/) - Publication Categories: ORIGINAL Beery ML, Jacobsen LM, Atkinson MA, Butler AE, Campbell-Thompson M. (2019) Islet Amyloidosis in a Child with Type 1 Diabetes. Islets. 2019;11(2):44-49.
- [Comprehensive Proteomics Analysis of Stressed Human Islets Identifies GDF15 as a Target for Type 1 Diabetes Intervention](https://npod.org/publication/comprehensive-proteomics-analysis-of-stressed-human-islets-identifies-gdf15-as-a-target-for-type-1-diabetes-intervention/) - Publication Categories: ORIGINAL Ernesto S. Nakayasu, Farooq Syed, Sarah A. Tersey, Marina A. Gritsenko, Hugh D. Mitchell, Chi Yuet Chan, Ercument Dirice, Jean-Valery Turatsinze, Yi Cui, Rohit N. Kulkarni, Decio L. Eizirik, Wei-Jun Qian, Bobbie-Jo M. Webb-Robertson, Carmella Evans-Molina, Raghavendra G. Mirmira, Thomas O. Metz, (2020). Comprehensive Proteomics Analysis of Stressed Human Islets Identifies GDF15 as
- [IL-6 is present in beta and alpha cells in human pancreatic islets: Expression is reduced in subjects with type 1 diabetes.](https://npod.org/publication/il-6-is-present-in-beta-and-alpha-cells-in-human-pancreatic-islets-expression-is-reduced-in-subjects-with-type-1-diabetes/) - Publication Categories: ORIGINAL Rajendran S, Anquetil F, Quesada-Masachs E, Graef M, Gonzalez N, McArdle S, Chu T, Krogvold L, Dahl-Jørgensen K, von Herrath M. (2019). IL-6 is present in beta and alpha cells in human pancreatic islets: Expression is reduced in subjects with type 1 diabetes. Clin Immunol. 2019 Dec 3:108320.
- [Human Endogenous Retroviruses and Type 1 Diabetes](https://npod.org/publication/human-endogenous-retroviruses-and-type-1-diabetes/) - Publication Categories: REVIEW Levet S, Charvet B, Bertin A, Deschaumes A, Perron H, Hober D. (2019). Human Endogenous Retroviruses and Type 1 Diabetes. Curr Diab Rep. 2019 Nov 21;19(12):141.
- [Phenotypic alterations in pancreatic lymph node stromal cells from human donors with type 1 diabetes and NOD mice](https://npod.org/publication/phenotypic-alterations-in-pancreatic-lymph-node-stromal-cells-from-human-donors-with-type-1-diabetes-and-nod-mice/) - Publication Categories: ORIGINAL Postigo-Fernandez, J., Farber, D.L. & Creusot, R.J. Diabetologia (2019) 62: 2040. https://doi.org/10.1007/s00125-019-04984-w
- [GLP-1 Receptor in Pancreatic α-Cells Regulates Glucagon Secretion in a Glucose-Dependent Bidirectional Manner](https://npod.org/publication/glp-1-receptor-in-pancreatic-α-cells-regulates-glucagon-secretion-in-a-glucose-dependent-bidirectional-manner/) - Publication Categories: ORIGINAL Zhang Y, Parajuli KR, Fava GE, Gupta R, Xu W, Nguyen LU, Zakaria AF, Fonseca VA, Wang H, Mauvais-Jarvis F, Sloop KW, Wu H. (2019). GLP-1 Receptor in Pancreatic α-Cells Regulates Glucagon Secretion in a Glucose-Dependent Bidirectional Manner. Diabetes. 2019 Jan;68(1):34-44.
- [The Juvenile Diabetes Research Foundation Network for Pancreatic Organ Donors with Diabetes (nPOD) Program: goals, operational model and emerging findings](https://npod.org/publication/6314/) - Publication Categories: REVIEW Pugliese A, Yang M, Kusmarteva I, Heiple T, Vendrame F, Wasserfall C, Rowe P, Moraski JM, Ball S, Jebson L, Schatz DA, Gianani R, Burke GW, Nierras C, Staeva T, Kaddis JS, Campbell-Thompson M, Atkinson MA. (2014) The Juvenile Diabetes Research Foundation Network for Pancreatic Organ Donors with Diabetes (nPOD) Program: goals,
- [Islet Interleukin-1β Immunoreactivity Is an Early Feature of Cystic Fibrosis That May Contribute to β-Cell Failure](https://npod.org/publication/islet-interleukin-1β-immunoreactivity-is-an-early-feature-of-cystic-fibrosis-that-may-contribute-to-β-cell-failure/) - Publication Categories: ORIGINAL Hull RL, Gibson RL, McNamara S, Deutsch GH, Fligner CL, Frevert CW, Ramsey BW, Sanda S. (2018) Islet Interleukin-1β Immunoreactivity Is an Early Feature of Cystic Fibrosis That May Contribute to β-Cell Failure. Diabetes Care. 2018 Apr;41(4):823-830.
- [Abnormal Islet Sphingolipid Metabolism in Type 1 Diabetes](https://npod.org/publication/abnormal-islet-sphingolipid-metabolism-in-type-1-diabetes/) - Publication Categories: ORIGINAL Holm LJ, Krogvold L, Hasselby JP, Kaur S, Claessens LA, Russell MA, Mathews CE, Hanssen KF, Morgan NG, Koeleman BPC, Roep BO, Gerling IC, Pociot F, Dahl-Jørgensen K, Buschard K. (2018) Abnormal Islet Sphingolipid Metabolism in Type 1 Diabetes. Diabetologia. 2018 Jul;61(7)
- [A Type 1 Diabetes Genetic Risk Score Predicts Progression of Islet Autoimmunity and Development of Type 1 Diabetes in Individuals at Risk](https://npod.org/publication/a-type-1-diabetes-genetic-risk-score-predicts-progression-of-islet-autoimmunity-and-development-of-type-1-diabetes-in-individuals-at-risk/) - Publication Categories: ORIGINAL
- [Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes](https://npod.org/publication/targeted-elimination-of-senescent-beta-cells-prevents-type-1-diabetes/) - Publication Categories: ORIGINAL
- [Low-Level Insulin Content Within Abundant Non-β Islet Endocrine Cells in Long-standing Type 1 Diabetes](https://npod.org/publication/low-level-insulin-content-within-abundant-non-β-islet-endocrine-cells-in-long-standing-type-1-diabetes/) - Publication Categories: ORIGINAL Lam CJ, Chatterjee A, Shen E, Cox AR, Kushner JA. (2019) Low-Level Insulin Content Within Abundant Non-β Islet Endocrine Cells in Long-standing Type 1 Diabetes. Diabetes. 2019 Mar;68(3):598-608.
- [Multiplexed In Situ Imaging Mass Cytometry Analysis of the Human Endocrine Pancreas and Immune System in Type 1 Diabetes](https://npod.org/publication/multiplexed-in-situ-imaging-mass-cytometry-analysis-of-the-human-endocrine-pancreas-and-immune-system-in-type-1-diabetes/) - Publication Categories: ORIGINAL Wang YJ, Traum D, Schug J, Gao L, Liu C; HPAP Consortium, Atkinson MA, Powers AC, Feldman MD, Naji A, Chang KM, Kaestner KH. (2019) Multiplexed In Situ Imaging Mass Cytometry Analysis of the Human Endocrine Pancreas and Immune System in Type 1 Diabetes. Cell Metab. 2019 Jan 28.
- [Proinsulin Secretion Is a Persistent Feature of Type 1 Diabetes](https://npod.org/publication/proinsulin-secretion-is-a-persistent-feature-of-type-1-diabetes/) - Publication Categories: ORIGINAL Sims EK, Bahnson HT, Nyalwidhe J, Haataja L, Davis AK, Speake C, DiMeglio LA, Blum J, Morris MA, Mirmira RG, Nadler J, Mastracci TL, Marcovina S, Qian WJ, Yi L, Swensen AC, Yip-Schneider M, Schmidt CM, Considine RV, Arvan P, Greenbaum CJ, Evans-Molina C; T1D Exchange Residual C-peptide Study Group (2019). Proinsulin Secretion
- [Nanowell-Mediated Two-Dimensional Liquid Chromatography Enables Deep Proteome Profiling of <1000 Mammalian Cells](https://npod.org/publication/nanowell-mediated-two-dimensional-liquid-chromatography-enables-deep-proteome-profiling-of/) - Publication Categories: ORIGINAL Dou M, Zhu Y, Liyu A, Liang Y, Chen J, Piehowski PD, Xu K, Zhao R, Moore RJ, Atkinson MA, Mathews CE, Qian WJ, Kelly RT. (2018) Nanowell-Mediated Two-Dimensional Liquid Chromatography Enables Deep Proteome Profiling of
- [Characterization of Non-hormone Expressing Endocrine Cells in Fetal and Infant Human Pancreas](https://npod.org/publication/characterization-of-non-hormone-expressing-endocrine-cells-in-fetal-and-infant-human-pancreas/) - Publication Categories: ORIGINAL Moin ASM, Montemurro C, Zeng K, Cory M, Nguyen M, Kulkarni S, Fritsch H, Meier JJ, Dhawan S, Rizza RA, Atkinson MA, Butler AE. (2019) Characterization of Non-hormone Expressing Endocrine Cells in Fetal and Infant Human Pancreas. Front Endocrinol (Lausanne). 2019 Jan 9;9:791.
- [Study of 2009 H1N1 Pandemic Influenza Virus as a Possible Causative Agent of Diabetes](https://npod.org/publication/study-of-2009-h1n1-pandemic-influenza-virus-as-a-possible-causative-agent-of-diabetes-2/) - Publication Categories: ORIGINAL Capua I, Mercalli A, Romero-Tejeda A, Pizzuto MS, Kasloff S, Sordi V, Marzinotto I, Lampasona V, Vicenzi E, De Battisti C, Bonfanti R, Rigamonti A, Terregino C, Doglioni C, Cattoli G, Piemonti L. (2018) Study of 2009 H1N1 Pandemic Influenza Virus as a Possible Causative Agent of Diabetes. J Clin Endocrinol Metab.
- [Beta Cells in Type 1 Diabetes: mass and function; sleeping or dead?](https://npod.org/publication/beta-cells-in-type-1-diabetes-mass-and-function-sleeping-or-dead/) - Publication Categories: ORIGINAL Oram RA, Sims EK, Evans-Molina C. (2019) Beta cells in type 1 diabetes: mass and function; sleeping or dead? Diabetologia. 2019 Feb 14.
- [Type I Interferon Is a Catastrophic Feature of the Diabetic Islet Microenvironment](https://npod.org/publication/type-i-interferon-is-a-catastrophic-feature-of-the-diabetic-islet-microenvironment/) - Publication Categories: REVIEW Newby BN, Mathews CE (2017). Type I Interferon Is a Catastrophic Feature of the Diabetic Islet Microenvironment. Front Endocrinol (Lausanne). 2017 Sep 14; 8:232.
- [Decreased α-cell mass and early structural alterations of the exocrine pancreas in patients with type 1 diabetes: an analysis based on the nPOD repository](https://npod.org/publication/decreased-α-cell-mass-and-early-structural-alterations-of-the-exocrine-pancreas-in-patients-with-type-1-diabetes-an-analysis-based-on-the-npod-repository-2/) - Publication Categories: ORIGINAL Bonnet-Serrano F, Diedisheim M, Mallone R, Larger E. (2018). Decreased α-cell mass and early structural alterations of the exocrine pancreas in patients with type 1 diabetes: An analysis based on the nPOD repository. PLoS One. 2018 Jan 19;13(1)
- [Intestinal Epithelial Cell Regulation of Adaptive Immune Dysfunction in Human Type 1 Diabetes](https://npod.org/publication/intestinal-epithelial-cell-regulation-of-adaptive-immune-dysfunction-in-human-type-1-diabetes/) - Publication Categories: ORIGINAL
- [Expansion of FasL-Expressing CD5+ B Cells in Type 1 Diabetes Patients](https://npod.org/publication/expansion-of-fasl-expressing-cd5-b-cells-in-type-1-diabetes-patients/) - Publication Categories: ORIGINAL Saxena A, Yagita H, Donner TW, Hamad ARA. (2018) Expansion of FasL-Expressing CD5+ B Cells in Type 1 Diabetes Patients. Front Immunol. 2017 Apr 7;8:402.
- [Loss of IDO1 Expression From Human Pancreatic β-Cells Precedes Their Destruction During the Development of Type 1 Diabetes](https://npod.org/publication/loss-of-ido1-expression-from-human-pancreatic-β-cells-precedes-their-destruction-during-the-development-of-type-1-diabetes/) - Publication Categories: ORIGINAL
- [Interferon-gamma drives programmed death-ligand 1 expression on islet β cells to limit T cell function during autoimmune diabetes](https://npod.org/publication/interferon-gamma-drives-programmed-death-ligand-1-expression-on-islet-β-cells-to-limit-t-cell-function-during-autoimmune-diabetes/) - Publication Categories: ORIGINAL
- [Organ Donor Pancreases for the Study of Human Islet Cell Histology and Pathophysiology: a precious and valuable resource](https://npod.org/publication/6460/) - Publication Categories: REVIEW Marchetti P, Suleiman M, Marselli L. (2018) Organ donor pancreases for the study of human islet cell histology and pathophysiology: a precious and valuable resource. Diabetologia. 2018 Apr;61(4):770-774.
- [Deciphering the Pathogenesis of Human Type 1 Diabetes (T1D) by Interrogating T Cells from the "Scene of the Crime"](https://npod.org/publication/deciphering-the-pathogenesis-of-human-type-1-diabetes-t1d-by-interrogating-t-cells-from-the-scene-of-the-crime/) - Publication Categories: REVIEW Kent SC, Mannering SI, Michels AW, Babon JAB. (2017). Deciphering the Pathogenesis of Human Type 1 Diabetes (T1D) by Interrogating T Cells from the "Scene of the Crime". Current Diabetes Reports. 2017 Sep 2; 17(10):95.
- [T Cell Receptor Profiling in Type 1 Diabetes](https://npod.org/publication/t-cell-receptor-profiling-in-type-1-diabetes/) - Publication Categories: REVIEW Jacobsen LM, Posgai A, Seay HR, Haller MJ, Brusko TM. (2017) T Cell Receptor Profiling in Type 1 Diabetes. Curr Diab Rep. 2017 Oct 11;17(11):118.
- [PDL1 is expressed in the islets of people with type 1 diabetes and is up-regulated by interferons-α and-γ via IRF1 induction](https://npod.org/publication/pdl1-is-expressed-in-the-islets-of-people-with-type-1-diabetes-and-is-up-regulated-by-interferons-α-and-γ-via-irf1-induction/) - Publication Categories: ORIGINAL Colli ML, Hill JLE, Marroquí L, Chaffey J, Dos Santos RS, Leete P, Coomans de Brachène A, Paula FMM, Op de Beeck A, Castela A, Marselli L, Krogvold L, Dahl-Jorgensen K, Marchetti P, Morgan NG, Richardson SJ, Eizirik DL. (2018). PDL1 is expressed in the islets of people with type 1 diabetes
- [Beta Cell DNA Damage Response Promotes Islet Inflammation in Type 1 Diabetes](https://npod.org/publication/beta-cell-dna-damage-response-promotes-islet-inflammation-in-type-1-diabetes/) - Publication Categories: ORIGINAL Horwitz E, Krogvold L, Zhitomirsky S, Swisa A, Fischman M, Lax T, Dahan T, Hurvitz N, Weinberg-Corem N, Klochendler A, Powers AC, Brissova M, Jörns A, Lenzen S, Glaser B, Dahl-Jørgensen K, Dor Y. (2018)Beta Cell DNA Damage Response Promotes Islet Inflammation in Type 1 Diabetes. Diabetes. 2018 Aug 27.
- [Cystic Fibrosis-related Diabetes is Caused by Islet Loss and Inflammation](https://npod.org/publication/cystic-fibrosis-related-diabetes-is-caused-by-islet-loss-and-inflammation/) - Publication Categories: ORIGINAL
- [Conventional and Neo-Antigenic Peptides Presented by β Cells Are Targeted by Circulating Naïve CD8+ T Cells in Type 1 Diabetic and Healthy Donors](https://npod.org/publication/conventional-and-neo-antigenic-peptides-presented-by-β-cells-are-targeted-by-circulating-naive-cd8-t-cells-in-type-1-diabetic-and-healthy-donors/) - Publication Categories: ORIGINAL
- [Unexpected Subcellular Distribution of a Specific Isoform of the Coxsackie and Adenovirus Receptor, CAR-SIV, in Human Pancreatic Beta Cells](https://npod.org/publication/unexpected-subcellular-distribution-of-a-specific-isoform-of-the-coxsackie-and-adenovirus-receptor-car-siv-in-human-pancreatic-beta-cells/) - Publication Categories: ORIGINAL Ifie E, Russell MA, Dhayal S, Leete P, Sebastiani G, Nigi L, Dotta F, Marjomäki V, Eizirik DL, Morgan NG, Richardson SJ. (2018) Unexpected subcellular distribution of a specific isoform of the Coxsackie and adenovirus receptor, CAR-SIV, in human pancreatic beta cells. Diabetologia. 2018 Aug 3.
- [Aberrant Menin Expression is an Early event in Pancreatic Neuroendocrine Tumorigenesis](https://npod.org/publication/aberrant-menin-expression-is-an-early-event-in-pancreatic-neuroendocrine-tumorigenesis/) - Publication Categories: ORIGINAL
- [Comparative Quantitative Proteomic Analysis of Disease Stratified Laser Captured Microdissected Human Islets Identifies Proteins and Pathways Potentially Related to Type 1 Diabetes](https://npod.org/publication/comparative-quantitative-proteomic-analysis-of-disease-stratified-laser-captured-microdissected-human-islets-identifies-proteins-and-pathways-potentially-related-to-type-1-diabetes/) - Publication Categories: ORIGINAL Nyalwidhe JO, Grzesik WJ, Burch TC, Semeraro ML, Waseem T, Gerling IC, Mirmira RD, Morris MA, Nadler JL. (2017) Comparative Quantitative Proteomic Analysis of Disease Stratified Laser Captured Microdissected Human Islets Identifies Proteins and Pathways Potentially Related to Type 1 Diabetes. PLoS One. 2017 Sep 6; 12(9):e0183908.
- [Virgin Beta Cells Persist throughout Life at a Neogenic Niche within Pancreatic Islets](https://npod.org/publication/virgin-beta-cells-persist-throughout-life-at-a-neogenic-niche-within-pancreatic-islets/) - Publication Categories: ORIGINAL Van der Meulen T, Mawla A, DiGruccio M, Adams M, Nies V, Dólleman S, Liu S, Ackermann A, Cácerres E, Hunter A, Kaestner K, Donaldson C, Huising M (2017). Virgin Beta Cells Persist throughout Life at a Neogenic Niche within Pancreatic Islets. Cell Metabolism. 2017 Apr 4; 25(4):911-926.
- [Highly Proliferative Alpha-Cell Related Islet Endocrine Cells in Human Pancreata](https://npod.org/publication/highly-proliferative-alpha-cell-related-islet-endocrine-cells-in-human-pancreata/) - Publication Categories: ORIGINAL
- [High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network](https://npod.org/publication/high-resolution-3d-imaging-of-the-human-pancreas-neuro-insular-network/) - Publication Categories: ORIGINAL Butterworth E, Dickerson W, Vijay V, Weitzel K, Cooper J, Atkinson EW, Coleman JE, Otto KJ, Campbell-Thompson M. (2018) High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network. J Vis Exp. 2018 Jan 29;(131).
- [Loss of Intra-Islet Heparan Sulfate is a Highly Sensitive Marker of Type 1 Diabetes Progression in Humans](https://npod.org/publication/loss-of-intra-islet-heparan-sulfate-is-a-highly-sensitive-marker-of-type-1-diabetes-progression-in-humans/) - Publication Categories: ORIGINAL Simeonovic CJ, Popp SK, Starrs LM, Brown DJ, Ziolkowski AF, Ludwig B, Bornstein SR, Wilson JD, Pugliese A, Kay TWH, Thomas HE, Loudovaris T, Choong FJ,Freeman C, Parish CR. (2018) Loss of intra-islet heparan sulfate is a highly sensitive marker of type 1 diabetes progression in humans. PLoS One. 2018 Feb 7;13(2):e0191360.
- [Islet Microvasculature Alterations With Loss of Beta-cells in Patients With Type 1 Diabetes](https://npod.org/publication/islet-microvasculature-alterations-with-loss-of-beta-cells-in-patients-with-type-1-diabetes/) - Publication Categories: ORIGINAL
- [Proteomic Analysis of Disease Stratified Human Pancreas Tissue Indicates Unique Signature of Type 1 Diabetes](https://npod.org/publication/proteomic-analysis-of-disease-stratified-human-pancreas-tissue-indicates-unique-signature-of-type-1-diabetes/) - Publication Categories: ORIGINAL Burch TC, Morris MA, Campbell-Thompson M, Pugliese A, Nadler JL, Nyalwidhe JO. (2015) Proteomic Analysis of Disease Stratified Human Pancreas Tissue Indicates Unique Signature of Type 1 Diabetes. PLOS ONE. 2015 Aug 24;10(8):e0135663.
- [Revealing Enterovirus Infection in Chronic Human Disorders: an integrated diagnostic approach](https://npod.org/publication/revealing-enterovirus-infection-in-chronic-human-disorders-an-integrated-diagnostic-approach/) - Publication Categories: ORIGINAL Genoni A, Canducci F, Rossi A, Broccolo F, Chumakov K, Bono G, Salerno-Uriarte J, Salvatoni A, Pugliese A, Toniolo A. (2017) Revealing enterovirus infection in chronic human disorders: An integrated diagnostic approach. Sci Rep. 2017 Jul 10;7(1):5013.
- [Immunohistochemical Assessment of Glucagon-Like Peptide 1 Receptor (GLP-1R) Expression in the Pancreas of Patients with Type 2 Diabetes](https://npod.org/publication/immunohistochemical-assessment-of-glucagon-like-peptide-1-receptor-glp-1r-expression-in-the-pancreas-of-patients-with-type-2-diabetes/) - Publication Categories: ORIGINAL Kirk R, Pyke C, von Herrath M, Hasselby J, Pedersen L, Mortensen P, Bjerre Knudsen L, Coppieters K (2017). Immunohistochemical Assessment of Glucagon-Like Peptide 1 Receptor (GLP-1R) Expression in the Pancreas of Patients with Type 2 Diabetes. Diabetes Obesity Metabolism. 2017 Jan 17.
- [Factors That Influence the Quality of RNA from the Pancreas of Organ Donors](https://npod.org/publication/factors-that-influence-the-quality-of-rna-from-the-pancreas-of-organ-donors/) - Publication Categories: ORIGINAL Philips T, Kusmartseva I, Gerling I, Campbell-Thompson M, Wasserfall C, Pugliese A, Longmate J, Schatz D, Atkinson M, Kaddis J (2017). Factors That Influence the Quality of RNA from the Pancreas of Organ Donors. Pancreas. 2017 Feb ;46(2):252-259.
- [An Immunohistochemical Study of Nitrotyrosine Expression in Pancreatic Islets of Cases with Increasing Duration of Type 1 Diabetes and without Diabetes](https://npod.org/publication/an-immunohistochemical-study-of-nitrotyrosine-expression-in-pancreatic-islets-of-cases-with-increasing-duration-of-type-1-diabetes-and-without-diabetes/) - Publication Categories: ORIGINAL
- [Germinal Centre Frequency is Decreased in Pancreatic Lymph Nodes from Individuals with Recent Onset Type 1 Diabetes](https://npod.org/publication/germinal-centre-frequency-is-decreased-in-pancreatic-lymph-nodes-from-individuals-with-recent-onset-type-1-diabetes/) - Publication Categories: ORIGINAL
- [β-Cells Persist in T1D Pancreata without Evidence for Ongoing β-Cell Turnover or Neogenesis](https://npod.org/publication/β-cells-persist-in-t1d-pancreata-without-evidence-for-ongoing-β-cell-turnover-or-neogenesis/) - Publication Categories: ORIGINAL Lam C, Jacobson D, Rankin M, Cox A, Kushner J (2017). β-Cells Persist in T1D Pancreata without Evidence for Ongoing β-Cell Turnover or Neogenesis. Journal of Clinical Endocrinology & Metabolism. 2017 Feb 27.
- [Alpha Cells, the Main Source of IL-1β in Human Pancreas](https://npod.org/publication/alpha-cells-the-main-source-of-il-1β-in-human-pancreas/) - Publication Categories: ORIGINAL Anquetil F, Sabouri S, Thivolet C, Rodriguez-Calvo T, Zapardiel-Gonzalo J, Amirian N, Schneider D, Castillo E, Lajevardi Y, von Herrath M (2017). Alpha Cells, the Main Source of IL-1β in Human Pancreas. Journal of Autoimmunity. 2017 Mar 18.
- [Pancreatic Nonhormone Expressing Endocrine Cells in Children with Type 1 Diabetes](https://npod.org/publication/pancreatic-nonhormone-expressing-endocrine-cells-in-children-with-type-1-diabetes/) - Publication Categories: ORIGINAL
- [β Cell Aging Markers Have Heterogeneous Distribution and Are Induced by Insulin Resistance](https://npod.org/publication/β-cell-aging-markers-have-heterogeneous-distribution-and-are-induced-by-insulin-resistance/) - Publication Categories: ORIGINAL Aguayo-Mazzucato C, van Haaren M, Mruk M, Lee T, Crawford C, Hollister-Lock J, Sullivan B, Johnson J, Ebrahimi A, Dreyfuss J, Van Deursen J, Weir G, Bonner-Weir S (2017). β Cell Aging Markers Have Heterogeneous Distribution and Are Induced by Insulin Resistance. Cell Metabolism. 2017 Apr 4;25(4):898-910.e5.
- [Advances in Pancreatic Islet Monolayer Culture on Glass Surfaces Enable Super-Resolution Microscopy and Insights into Beta Cell Ciliogenesis and Prolifertation](https://npod.org/publication/advances-in-pancreatic-islet-monolayer-culture-on-glass-surfaces-enable-super-resolution-microscopy-and-insights-into-beta-cell-ciliogenesis-and-prolifertation/) - Publication Categories: ORIGINAL
- [High Illicit Drug Abuse and Suicide in Organ Donors with Type 1 Diabetes](https://npod.org/publication/high-illicit-drug-abuse-and-suicide-in-organ-donors-with-type-1-diabetes/) - Publication Categories: ORIGINAL Jacobsen LM, Haller MJ, Parish A, Gurka MJ, Levine SR, Wasserfall C, Campbell-Thompson M, Kaddis J, Pugliese A, Atkinson MA, Schatz DA. (2017) High Illicit Drug Abuse and Suicide in Organ Donors with Type 1 Diabetes. Diabetes Care. 2017 Jul 5.
- [β-Cell mass versus function in type 1 diabetes mellitus: truth or dare?](https://npod.org/publication/β-cell-mass-versus-function-in-type-1-diabetes-mellitus-truth-or-dare/) - Publication Categories: REVIEW Rodriguez-Calvo T, Atkinson M, von Herrath M. (2017) β-Cell mass versus function in type 1 diabetes mellitus: truth or dare? Nat Rev Endocrinol. 2017 Jul 7.
- [Islet Inflammation and Ductal Proliferation May be Linked to Increased Pancreatitis Risk in Type 2 Diabetes](https://npod.org/publication/islet-inflammation-and-ductal-proliferation-may-be-linked-to-increased-pancreatitis-risk-in-type-2-diabetes/) - Publication Categories: ORIGINAL
- [Reduction of Endoplasmic Reticulum-Mitochondria Interactions in Beta Cells from Patients with Type 2 Diabetes](https://npod.org/publication/reduction-of-endoplasmic-reticulum-mitochondria-interactions-in-beta-cells-from-patients-with-type-2-diabetes/) - Publication Categories: ORIGINAL
- [Neuropeptide Y Expression Marks Partially Differentiated β Cells in Mice and Humans](https://npod.org/publication/neuropeptide-y-expression-marks-partially-differentiated-β-cells-in-mice-and-humans/) - Publication Categories: ORIGINAL Rodnoi P, Rajkumar M, Moin ASM, Georgia SK, Butler, AE, Dhawan S. (2017). Neuropeptide Y Expression Marks Partially Differentiated β Cells in Mice and Humans. JCI Insight. 2017 Jun 15; 2(12).
- [Regulatory T-cells from Pancreatic Lymphnodes of Patients with Type-1 Diabetes Express Increased Levels of MicroRNA miR-125a-5p that Limits CCR2 Expression](https://npod.org/publication/regulatory-t-cells-from-pancreatic-lymphnodes-of-patients-with-type-1-diabetes-express-increased-levels-of-microrna-mir-125a-5p-that-limits-ccr2-expression/) - Publication Categories: ORIGINAL Sebastiani G, Ventriglia G, Stabilini A, Socci C, Morsiani C, Laurenzi A, Nigi L, Formichi C, Mfarrej B, Petrelli A, Fousteri G, Brusko TM, Dotta F, Battaglia M. (2017) Regulatory T-cells from pancreatic lymphnodes of patients with type-1 diabetes express increased levels of microRNA miR-125a-5p that limits CCR2 expression. Sci Rep. 2017
- [Autoreactive T Cells in Type 1 Diabetes](https://npod.org/publication/autoreactive-t-cells-in-type-1-diabetes/) - Publication Categories: REVIEW Pugliese, A. (2017). Autoreactive T Cells in Type 1 Diabetes. J Clin Invest. 2017 Aug 1;127(8):2881-2891.
- [Persistence of Pancreatic Insulin mRNA Expression and Proinsulin Protein in Type 1 Diabetes Pancreata](https://npod.org/publication/persistence-of-pancreatic-insulin-mrna-expression-and-proinsulin-protein-in-type-1-diabetes-pancreata/) - Publication Categories: ORIGINAL Wasserfall C, Nick HS, Campbell-Thompson M, Beachy D, Haataja L, Kusmartseva I, Posgai A, Beery M, Rhodes C, Bonifacio E, Arvan P, Atkinson M. (2007) Persistence of Pancreatic Insulin mRNA Expression and Proinsulin Protein in Type 1 Diabetes Pancreata. Cell Metab. 2017 Sep 5;26(3):568-575.e3.
- [Neuropeptide FF Increases M2 Activation and Self-Renewal of Adipose Tissue Macrophages](https://npod.org/publication/neuropeptide-ff-increases-m2-activation-and-self-renewal-of-adipose-tissue-macrophages/) - Publication Categories: ORIGINAL Syed F. Hassnain Waqas, Anh Cuong Hoang, Ya-Tin Lin, Grace Ampem, Hind Azegrouz, Lajos Balogh, Julianna Thuróczy, Jin-Chung Chen, Ivan C. Gerling, Sorim Nam, Jong-Seok Lim, Juncal Martinez-Ibañez, José T. Real, Stephan Paschke, Raphaëlle Quillet, Safia Ayachi, Frédéric Simonin, E. Marion Schneider, Jacqueline A. Brinkman, Dudley W. Lamming, Christine M. Seroogy, and
- [The Four-Way Stop Sign: Viruses, 12-Lipoxygenase, Islets, and Natural Killer Cells in Type 1 Diabetes Progression](https://npod.org/publication/the-four-way-stop-sign-viruses-12-lipoxygenase-islets-and-natural-killer-cells-in-type-1-diabetes-progression/) - Publication Categories: ORIGINAL
- [Hospital Time Prior to Death and Pancreas Histopathology: implications for future studies](https://npod.org/publication/hospital-time-prior-to-death-and-pancreas-histopathology-implications-for-future-studies/) - Publication Categories: ORIGINAL
- [Structural Abnormalities in Islets from Very Young Children with Cystic Fibrosis May Contribute to Cystic Fibrosis Related Diabetes](https://npod.org/publication/structural-abnormalities-in-islets-from-very-young-children-with-cystic-fibrosis-may-contribute-to-cystic-fibrosis-related-diabetes/) - Publication Categories: ORIGINAL
- [Response to Comment on Jackson et al. Insulitis in Autoantibody-Positive Pancreatic Donor With History of Gestational Diabetes Mellitus](https://npod.org/publication/response-to-comment-on-jackson-et-al-insulitis-in-autoantibody-positive-pancreatic-donor-with-history-of-gestational-diabetes-mellitus/) - Publication Categories: REVIEW Jackson J, Posgai A, Campbell-Thompson M, Kusmartseva I. (2017) Response to Comment on Jackson et al. Insulitis in Autoantibody-Positive Pancreatic Donor With History of Gestational Diabetes Mellitus. Diabetes Care. 2017 Oct;40(10):e156.
- [Insulitis in Autoantibody-Positive Pancreatic Donor With History of Gestational Diabetes Mellitus](https://npod.org/publication/insulitis-in-autoantibody-positive-pancreatic-donor-with-history-of-gestational-diabetes-mellitus/) - Publication Categories: ORIGINAL
- [Beta-Cell Mass in Non-Diabetic Autoantibody-Positive Subjects: an analysis based on the nPOD database](https://npod.org/publication/beta-cell-mass-in-non-diabetic-autoantibody-positive-subjects-an-analysis-based-on-the-npod-database/) - Publication Categories: ORIGINAL Diedisheim M, Mallone R, Boitard C, Larger E. (2016) Beta-cell mass in non-diabetic autoantibody-positive subjects: an analysis based on the nPOD database. The Journal of Clinical Endocrinology and Metabolism. 2016 Feb 1.
- [Differential Insulitic Profiles Determine the Extent of Beta Cell Destruction and the Age at Onset of Type 1 Diabetes](https://npod.org/publication/differential-insulitic-profiles-determine-the-extent-of-beta-cell-destruction-and-the-age-at-onset-of-type-1-diabetes/) - Publication Categories: ORIGINAL Leete P, Willcox A, Krogvold L, Dahl-Jørgensen K, Foulis AK, Richardson SJ, Morgan NG (2016). Differential insulitic profiles determine the extent of beta cell destruction and the age at onset of type 1 diabetes. Diabetes. 2016 Feb 8.
- [Pancreatic Duct Hyperplasia/dysplasia in Type 1 Diabetes and Pancreatic Weight in Individuals with and without Diabetes](https://npod.org/publication/pancreatic-duct-hyperplasiadysplasia-in-type-1-diabetes-and-pancreatic-weight-in-individuals-with-and-without-diabetes/) - Publication Categories: REVIEW Campbell-Thompson ML, Schatz DA, Kaddis JS, Atkinson MA (2016). Pancreatic duct hyperplasia/dysplasia in type 1 diabetes and pancreatic weight in individuals with and without diabetes. Reply to Kobayashi T, Aida K, Fukui T et al [letter] and Saisho Y [letter]. Diabetologia. 2016 Apr;59(4):870-2.
- [Lipids and Immunoinflammatory Pathways of Beta Cell Destruction](https://npod.org/publication/lipids-and-immunoinflammatory-pathways-of-beta-cell-destruction/) - Publication Categories: ORIGINAL Imai Y, Dobrian A, Morris A, Taylor-Fishwick D, Nadler J (2016). Lipids and immunoinflammatory pathways of beta cell destruction. Diabetologia. 2016 Apr;59(4):673-8.
- [Viral Infections in Type 1 Diabetes Mellitus - Why the β Cells?](https://npod.org/publication/viral-infections-in-type-1-diabetes-mellitus-why-the-β-cells/) - Publication Categories: REVIEW Op de beeck A, Eizirik D (2016). Viral Infections in Type 1 Diabetes Mellitus - Why the β Cells? Nature Reviews Endocrinology. 2016 Mar.
- [Increased Hormone-Negative Endocrine Cells in the Pancreas in Type 1 Diabetes](https://npod.org/publication/increased-hormone-negative-endocrine-cells-in-the-pancreas-in-type-1-diabetes/) - Publication Categories: ORIGINAL Md Moin AS, Dhawan S, Shieh C, Butler PC, Cory M, Butler AE (2016). Increased hormone-negative endocrine cells in the pancreas in type 1 diabetes. J Clin Endocrinol Metab. 2016 Jun; 14:jc20161350.
- [Presumptive Type 1 Diabetes with Comorbidities and Rapid Progression Despite Numerous Insulin-Positive Islets](https://npod.org/publication/presumptive-type-1-diabetes-with-comorbidities-and-rapid-progression-despite-numerous-insulin-positive-islets/) - Publication Categories: ORIGINAL Jacobsen L, Atkinson M, Campbell-Thompson M, Schatz D (2016). Presumptive Type 1 Diabetes With Comorbidities and Rapid Progression Despite Numerous Insulin-Positive Islets. Diabetes Care. 2016 July; 39(7): 1292-4.
- [Aberrant Accumulation of the Diabetes Autoantigen GAD65 in Golgi Membranes in Conditions of ER Stress and Autoimmunity](https://npod.org/publication/6404/) - Publication Categories: ORIGINAL Phelps EA, Cianciaruso C, Michael IP, Pasquier M, Kanaani J, Nano R, Lavallard V, Billestrup N, Hubbell JA, Baekkeskov S (2016). Aberrant accumulation of the diabetes autoantigen GAD65 in Golgi membranes in conditions of ER stress and autoimmunity. Diabetes. 2016 Jun 9.
- [Evaluation of Immunohistochemical Staining for Glucagon in Human Pancreatic Tissue](https://npod.org/publication/evaluation-of-immunohistochemical-staining-for-glucagon-in-human-pancreatic-tissue/) - Publication Categories: ORIGINAL Gurlo T, Butler PC, Butler AE (2016). Evaluation of immunohistochemical staining for glucagon in human pancreatic tissue. J Histotechnology. 2016 May 11; 39(1):8-16.
- [Thinking Outside the Cell: a key role for hyaluronan in the pathogenesis of human type 1 diabetes](https://npod.org/publication/thinking-outside-the-cell-a-key-role-for-hyaluronan-in-the-pathogenesis-of-human-type-1-diabetes/) - Publication Categories: ORIGINAL Bogdani M (2016). Thinking Outside the Cell: A Key Role for Hyaluronan in the Pathogenesis of Human Type 1 Diabetes. Diabetes. 2016 August; 65(8): 2105-2114.
- [Validation of a Rapid Type 1 Diabetes Autoantibody Screening Assay for Community-based Screening of Organ Donors to Identify Subjects at Increased Risk for the Disease](https://npod.org/publication/validation-of-a-rapid-type-1-diabetes-autoantibody-screening-assay-for-community-based-screening-of-organ-donors-to-identify-subjects-at-increased-risk-for-the-disease/) - Publication Categories: ORIGINAL Wasserfall C, Montgomery E, Yu L, Michels A, Gianani R, Pugliese A, Nierras C, Kaddis J, Schatz DA, Bonifacio E, Atkinson M (2016). Validation of a rapid type 1 diabetes autoantibody screening assay for community-based screening of organ donors to identify subjects at increased risk for the disease. Clinical & Experimental Immunology.
- [Raising Awareness: the need to promote allocation of pancreata from rare non-diabetic donors with pancreatic islet autoimmunity to type 1 diabetes research](https://npod.org/publication/raising-awareness-the-need-to-promote-allocation-of-pancreata-from-rare-non-diabetic-donors-with-pancreatic-islet-autoimmunity-to-type-1-diabetes-research/) - Publication Categories: ORIGINAL Burke GW 3rd, Posgai A, Wasserfall C, Atkinson M, Pugliese A (2016). Raising Awareness: The Need to Promote Allocation of Pancreata from Rare Non-Diabetic Donors with Pancreatic Islet Autoimmunity to Type 1 Diabetes Research. American Journal of Transplantation. 2016 Jul 26.
- [Insulitis in the Pathogenesis of Type 1 Diabetes](https://npod.org/publication/insulitis-in-the-pathogenesis-of-type-1-diabetes/) - Publication Categories: ORIGINAL Pugliese A (2016). Insulitis in the pathogenesis of type 1 diabetes. Pediatric Diabetes. 2016 Jul; 17 Suppl 22:31-6.
- [Type 1 Diabetes Cadaveric Human Pancreata Exhibit a Unique Exocrine Tissue Proteomic Profile](https://npod.org/publication/type-1-diabetes-cadaveric-human-pancreata-exhibit-a-unique-exocrine-tissue-proteomic-profile/) - Publication Categories: ORIGINAL Liu CW, Atkinson M, Zhang Q (2016). Type 1 diabetes cadaveric human pancreata exhibit a unique exocrine tissue proteomic profile. Proteomics. 2016 May; 16(9): 1432-46.
- [Relative Sensitivity of Immunohistochemistry, Multiple Reaction Monitoring Mass Spectrometry, in situ Hybridization and PCR to Detect Coxsackievirus B1 in A549 Cells](https://npod.org/publication/relative-sensitivity-of-immunohistochemistry-multiple-reaction-monitoring-mass-spectrometry-in-situ-hybridization-and-pcr-to-detect-coxsackievirus-b1-in-a549-cells/) - Publication Categories: ORIGINAL Laiho JE, Oikarinen M, Richardson SJ, Frisk G, Nyalwidhe J, Burch TC, Morris MA, Oikarinen S, Pugliese A, Dotta F, Campbell-Thompson M, Nadler J, Morgan NG, Hyoty H, nPOD-Virus Group (2016). Relative sensitivity of immunohistochemistry, multiple reaction monitoring mass spectrometry, in situ hybridization and PCR to detect Coxsackievirus B1 in A549 cells.
- [The Immune Pathogenesis of Type 1 Diabetes: not only thinking outside the cell but also outside the islet and out of the box](https://npod.org/publication/the-immune-pathogenesis-of-type-1-diabetes-not-only-thinking-outside-the-cell-but-also-outside-the-islet-and-out-of-the-box/) - Publication Categories: ORIGINAL Drexhage H, Dik W, Leenen P, Versnel M (2016). The Immune Pathogenesis of Type 1 Diabetes: Not Only Thinking Outside the Cell but Also Outside the Islet and Out of the Box. Diabetes. 2016 Aug; 65(8):2103-3.
- [Islet Cell Hyperexpression of HLA Class I Antigens: a defining feature in type 1 diabetes](https://npod.org/publication/islet-cell-hyperexpression-of-hla-class-i-antigens-a-defining-feature-in-type-1-diabetes/) - Publication Categories: ORIGINAL Richardson SJ, Rodriguez-Calvo T, Gerling IC, Mathews CE, Kaddis JS, Russell MA, Zeissler M, Leete P, Dahl-Jorgensen K, von Herrath M, Pugliese A, Atkinson M, Morgan NG (2016). Islet cell hyperexpression of HLA class I antigens: a defining feature in type 1 diabetes. Diabetologia. 2016 Aug 9.
- [Diagnostic Dilemma: clinical and histological abnormalities in a Hispanic patient with diabetes](https://npod.org/publication/diagnostic-dilemma-clinical-and-histological-abnormalities-in-a-hispanic-patient-with-diabetes/) - Publication Categories: ORIGINAL Jacobsen L, Posgai A, Campbell-Thompson M, Schatz D (2016). Diagnostic Dilemma: Clinical and Histological Abnormalities in a Hispanic Patient With Diabetes. Diabetes Care. 2016 Sep; 39(9): 1650-2.
- [Team Science in Type 1 Diabetes: new insights from the Network for Pancreatic Organ Donors with Diabetes](https://npod.org/publication/team-science-in-type-1-diabetes-new-insights-from-the-network-for-pancreatic-organ-donors-with-diabetes/) - Publication Categories: ORIGINAL Lanzoni G, Pokrywczynska M, Inverardi L (2016). Team Science in Type 1 Diabetes: New Insights from the Network for Pancreatic Organ Donors with Diabetes (nPOD). CellR4. 2016; 4 (5): e2128.
- [Tracking the Antibody Immunome in Type 1 Diabetes Using Protein Arrays](https://npod.org/publication/tracking-the-antibody-immunome-in-type-1-diabetes-using-protein-arrays/) - Publication Categories: ORIGINAL Bian X, Wasserfall C, Wallstrom G, Wang J, Wang H, Barker K, Schatz D, Atkinson M, Qiu J, LaBaer J (2016). Tracking the Antibody Immunome in Type 1 Diabetes Using Protein Arrays. J Proteome Res. 2016 Oct 3.
- [PAR2 Regulates Regeneration, Trasndifferentiation, and Death](https://npod.org/publication/par2-regulates-regeneration-trasndifferentiation-and-death/) - Publication Categories: ORIGINAL Piran R, Lee S, Kuss P, Hao E, Newlin E, Millán J, Levine F (2016). PAR2 regulates regeneration, trasndifferentiation, and death. Cell Death & Disease. 2016 Nov 3.
- [Tissue Distribution and Clonal Diversity of the T and B Cell Repertoire in Type 1 Diabetes](https://npod.org/publication/tissue-distribution-and-clonal-diversity-of-the-t-and-b-cell-repertoire-in-type-1-diabetes-2/) - Publication Categories: ORIGINAL
- [The Plasma Membrane Metal-ion Transporter ZIP14 Contributes to non-transferrin-bound Iron Uptake by Human β Cells](https://npod.org/publication/the-plasma-membrane-metal-ion-transporter-zip14-contributes-to-non-transferrin-bound-iron-uptake-by-human-β-cells/) - Publication Categories: ORIGINAL Coffey R, Knutson MD (2016). The plasma membrane metal-ion transporter ZIP14 contributes to non-transferrin-bound iron uptake by human β cells. Am J Physiol Cell Physiol. 2016 Nov 30:ajpcell.00116.2016.
- [Properties of Two Enterovirus Antibodies that are Utilized in Diabetes Research](https://npod.org/publication/properties-of-two-enterovirus-antibodies-that-are-utilized-in-diabetes-research/) - Publication Categories: ORIGINAL Maccari G, Genoni AP, Sansonno S, Toniolo A (2016). Properties of two enterovirus antibodies that are utilized in diabetes research. Sci. Rep. 2016; 6, 24757.
- [Stereological Analyses of the Whole Human Pancreas](https://npod.org/publication/stereological-analyses-of-the-whole-human-pancreas/) - Publication Categories: ORIGINAL Poudel A, Fowler JL, Zielinski MC, Kilimnik G, Hara M. (2016) Stereological analyses of the whole human pancreas. Sci Rep. 2016 Sep 23;6:34049.
- [Analysis of Peri-Islet CD45-Positive Leucocytic Infiltrates in Long-Standing Type 1 Diabetic Patients](https://npod.org/publication/analysis-of-peri-islet-cd45-positive-leucocytic-infiltrates-in-long-standing-type-1-diabetic-patients/) - Publication Categories: ORIGINAL
- [The Streetlight Effect in Type 1 Diabetes](https://npod.org/publication/the-streetlight-effect-in-type-1-diabetes/) - Publication Categories: ORIGINAL Battaglia M, Atkinson MA. (2015) The Streetlight Effect in Type 1 Diabetes. Diabetes. 2015 Apr;64(4):1081-90.
- [Urocortin3 Mediates Somatostatin-dependent Negative Feedback Control of Insulin Secretion](https://npod.org/publication/urocortin3-mediates-somatostatin-dependent-negative-feedback-control-of-insulin-secretion/) - Publication Categories: ORIGINAL Talitha van der Meulen, Cynthia J Donaldson, Elena Cáceres, Anna E Hunter, Christopher Cowing-Zitron, Lynley D Pound, Michael W Adams, Andreas Zembrzycki, Kevin L Grove, Mark O Huising. (2015) Urocortin3 mediates somatostatin-dependent negative feedback control of insulin secretion. Nature Medicine. 2015 Jun 15.
- [The Detection of Glycosaminoglycans in Pancreatic Islets and Lymphoid Tissues](https://npod.org/publication/the-detection-of-glycosaminoglycans-in-pancreatic-islets-and-lymphoid-tissues/) - Publication Categories: ORIGINAL Bogdani M, Simeonovic C, Nagy N, Johnson PY, Chan CK, Wight TN. (2015) The detection of glycosaminoglycans in pancreatic islets and lymphoid tissues. Methods in Molecular Biology. 2015;1229:413-30.
- [Application of Bioinformatics in Probe Design Enables Detection of Enteroviruses on Different Taxonomic Levels by Advanced in situ Hybridization Technology](https://npod.org/publication/application-of-bioinformatics-in-probe-design-enables-detection-of-enteroviruses-on-different-taxonomic-levels-by-advanced-in-situ-hybridization-technology/) - Publication Categories: ORIGINAL Jutta E. Laiho, Sami Oikarinenemail, Maarit Oikarinenemail, Pär Larssonemail, Virginia M. Stoneemail, Didier Hoberemail, Steven Obersteemail, Malin Flodström-Tullbergemail, Jorma Isolaemail, Heikki Hyötyemail. (2015) Application of bioinformatics in probe design enables detection of enteroviruses on different taxonomic levels by advanced in situ hybridization technology. Journal of Clinical Virology. 2015 Jun 23.
- [Increased Expression of IL-18 in the Serum and Islets of Type 1 Diabetics](https://npod.org/publication/increased-expression-of-il-18-in-the-serum-and-islets-of-type-1-diabetics/) - Publication Categories: ORIGINAL Robert Z. Harmsa, Danielle N. Yardea, Zachary Guinna, Kristina M. Lorenzo-Arteagaa, Kevin P. Corleyb, Monina S. Cabrerab, Nora E. Sarvetnick. (2015) Increased expression of IL-18 in the serum and islets of type 1 diabetics. Molecular Immunology. 2015 April.
- [Organ Donor Specimens: what can they tell us about type 1 diabetes?](https://npod.org/publication/organ-donor-specimens-what-can-they-tell-us-about-type-1-diabetes/) - Publication Categories: ORIGINAL Campbell-Thompson M. (2015) Organ donor specimens: What can they tell us about type 1 diabetes? Pediatric Diabetes. 2015 Aug;16(5):320-30.
- [The Subcellular Distribution of Cyclin-D1 and Cyclin-D3 Within Human Islet Cells Varies According to the Status of the Pancreas Donor](https://npod.org/publication/the-subcellular-distribution-of-cyclin-d1-and-cyclin-d3-within-human-islet-cells-varies-according-to-the-status-of-the-pancreas-donor/) - Publication Categories: ORIGINAL Taniguchi K, Russell MA, Richardson SJ, Morgan NG. (2015) The subcellular distribution of cyclin-D1 and cyclin-D3 within human islet cells varies according to the status of the pancreas donor. Diabetologia. 2015 Sep;58(9):2056-63.
- [Abnormalities of the Exocrine Pancreas in Type 1 Diabetes](https://npod.org/publication/abnormalities-of-the-exocrine-pancreas-in-type-1-diabetes/) - Publication Categories: ORIGINAL
- [The Influence of Type 1 Diabetes on Pancreatic Weight](https://npod.org/publication/the-influence-of-type-1-diabetes-on-pancreatic-weight/) - Publication Categories: ORIGINAL Campbell-Thompson ML, Kaddis JS, Wasserfall C, Haller MJ, Pugliese A, Schatz DA, Shuster JJ, Atkinson MA. (2015) The influence of type 1 diabetes on pancreatic weight. Diabetologia. 2015 Sep 10. (Featured on the January 2016 cover of Diabetologia)
- [Inhibition of Hyaluronan Synthesis Restores Immune Tolerance During Autoimmune Insulitis](https://npod.org/publication/inhibition-of-hyaluronan-synthesis-restores-immune-tolerance-during-autoimmune-insulitis/) - Publication Categories: ORIGINAL Nadine Nagy, Gernot Kaber, Pamela Y. Johnson, John A. Gebe, Anton Preisinger, Ben A. Falk, Vivekananda G. Sunkari, Michel D. Gooden, Robert B. Vernon, Marika Bogdani, Hedwich F. Kuipers, Anthony J. Day, Daniel J. Campbell, Thomas N. Wight, and Paul L. Bollyky. (2015) Inhibition of hyaluronan synthesis restores immune tolerance during autoimmune
- [Beyond the Brain: disrupted in schizophrenia 1 regulates pancreatic β-cell function via glycogen synthase kinase-3β](https://npod.org/publication/beyond-the-brain-disrupted-in-schizophrenia-1-regulates-pancreatic-β-cell-function-via-glycogen-synthase-kinase-3β/) - Publication Categories: ORIGINAL
- [Insulitis and β-Cell Mass in the Natural History of Type 1 Diabetes](https://npod.org/publication/insulitis-and-β-cell-mass-in-the-natural-history-of-type-1-diabetes/) - Publication Categories: ORIGINAL Campbell-Thompson M, Fu A, Kaddis JS, Wasserfall C, Schatz DA, Pugliese A, Atkinson MA. (2015) Insulitis and β-Cell Mass in the Natural History of Type 1 Diabetes. Diabetes. 2015 Nov 18. pii: db150779. [Epub ahead of print]. (Featured on the March 2016 cover of Diabetes)
- [A Run on the Biobank: what have we learned about type 1 diabetes from the nPOD tissue repository?](https://npod.org/publication/a-run-on-the-biobank-what-have-we-learned-about-type-1-diabetes-from-the-npod-tissue-repository/) - Publication Categories: ORIGINAL Kaddis JS, Pugliese A, Atkinson MA (2015). A run on the biobank: what have we learned about type 1 diabetes from the nPOD tissue repository? Current Opinion in Endocrinology & Diabetes and Obesity. 2015 Aug; 22(4):290-5.
- [Viral Diabetes: virus diabetogenicity and host susceptibility](https://npod.org/publication/viral-diabetes-virus-diabetogenicity-and-host-susceptibility/) - Publication Categories: ORIGINAL Nagafuchi S, Toniolo A (2015). Viral diabetes: virus diabetogenicity and host susceptibility. Immunoendocrinology 2015; 2: e1026.
- [From Non-obese Diabetic to Network for the Pancreatic Organ Donors with Diabetes: new heights in type 1 diabetes research](https://npod.org/publication/from-non-obese-diabetic-to-network-for-the-pancreatic-organ-donors-with-diabetes-new-heights-in-type-1-diabetes-research/) - Publication Categories: ORIGINAL Ramirez L, Hamad AR (2015). From non-obese diabetic to Network for the Pancreatic Organ Donors with Diabetes: new heights in type 1 diabetes research. World Journal of Diabetes. 2015 Nov 25;6(16):1309-11.
- [Extracellular Matrix Components in the Pathogenesis of Type 1 Diabetes](https://npod.org/publication/extracellular-matrix-components-in-the-pathogenesis-of-type-1-diabetes/) - Publication Categories: ORIGINAL Bogdani M, Korpos E, Simeonovic CJ, Parish CR, Sorokin L, Wight TN. (2014) Extracellular matrix components in the pathogenesis of type 1 diabetes. Current Diabetes Reports.
- [What is the Role of Autoimmunity in Type 1 Diabetes? A Clinical Perspective](https://npod.org/publication/what-is-the-role-of-autoimmunity-in-type-1-diabetes-a-clinical-perspective/) - Publication Categories: REVIEW Donath MY, Hess C, Palmer E. (2015) What is the role of autoimmunity in type 1 diabetes? A clinical perspective. Diabetologia.
- [Islet Inflammation in Human Type 1 Diabetes Mellitus](https://npod.org/publication/islet-inflammation-in-human-type-1-diabetes-mellitus/) - Publication Categories: ORIGINAL Morgan NG, Leete P, Foulis AK, Richardson SJ. (2014) Islet inflammation in human type 1 diabetes mellitus. IUBMB Life.
- [Self-antigen Expression in the Peripheral Immune System: roles in self-tolerance and type 1 diabetes pathogenesis](https://npod.org/publication/self-antigen-expression-in-the-peripheral-immune-system-roles-in-self-tolerance-and-type-1-diabetes-pathogenesis/) - Publication Categories: ORIGINAL Fuhlbrigge R, and Yip L (2014) Self-antigen expression in the peripheral immune system: roles in self-tolerance and type 1 diabetes pathogenesis. Current Diabetes Report.
- [Type 1 Diabetes in Mice and Men: gene expression profiling to investigate disease pathogenesis](https://npod.org/publication/type-1-diabetes-in-mice-and-men-gene-expression-profiling-to-investigate-disease-pathogenesis/) - Publication Categories: ORIGINAL Yip L, and Fathman CG (2014) Type 1 diabetes in mice and men: gene expression profiling to investigate disease pathogenesis. Immunologic Research.
- [Expression Pattern of 12-lipoxygenases in Human Islets with Type 1 Diabetes and Type 2 Diabetes](https://npod.org/publication/expression-pattern-of-12-lipoxygenases-in-human-islets-with-type-1-diabetes-and-type-2-diabetes/) - Publication Categories: ORIGINAL Grzesik WJ, Nadler Joseph L., Machida Y, Jerry L. Nadler, Imai Y, and Morris MA. (2014) Expression pattern of 12-lipoxygenases in human islets with type 1 diabetes and type 2 diabetes. The Journal of Clinical Endocrinology & Metabolism.
- [Detection of a Low-grade Enteroviral Infection in the Islets of Langerhans of Living Patients Newly Diagnosed with Type 1 Diabetes](https://npod.org/publication/detection-of-a-low-grade-enteroviral-infection-in-the-islets-of-langerhans-of-living-patients-newly-diagnosed-with-type-1-diabetes/) - Publication Categories: ORIGINAL Krogvold L, Edwin B, Buanes T, Frisk G, Skog O, Anagandula M, Korsgren O, Undlien D, Eike M, Richardson SJ, Leete P, Morgan NG, Oikarinen S, Oikarinen M, Laiho JE, Hyöty H, Ludvigsson J, Hanssen KF, Dahl-Jørgensen K. (2014) Detection of a low-grade enteroviral infection in the islets of Langerhans of living
- [Summary of the Keystone Islet Workshop: the increasing demand for human islet availability in diabetes research](https://npod.org/publication/summary-of-the-keystone-islet-workshop-the-increasing-demand-for-human-islet-availability-in-diabetes-research/) - Publication Categories: REVIEW Kulkarni RN, Stewart AF (2014) Summary of the keystone islet workshop (april 2014): the increasing demand for human islet availability in diabetes research. Diabetes.
- [Losing a Grip on the Notion of β-Cell Specificity for Immune Responses in Type 1 Diabetes: can we handle the truth?](https://npod.org/publication/losing-a-grip-on-the-notion-of-β-cell-specificity-for-immune-responses-in-type-1-diabetes-can-we-handle-the-truth/) - Publication Categories: REVIEW Atkinson, MA (2014) Losing a Grip on the Notion of β-Cell Specificity for Immune Responses in Type 1 Diabetes: Can We Handle the Truth? Diabetes.
- [Characterizing Subgroups of Type 1 Diabetes](https://npod.org/publication/6326/) - Publication Categories: REVIEW Skyler, JS (2014) Characterizing subgroups of type 1 diabetes. Diabetes.
- [Islet Autoimmunity Identifies a Unique Pattern of Impaired Pancreatic Beta-Cell Function, Markedly Reduced Pancreatic Beta Cell Mass and Insulin Resistance in Clinically Diagnosed Type 2 Diabetes](https://npod.org/publication/islet-autoimmunity-identifies-a-unique-pattern-of-impaired-pancreatic-beta-cell-function-markedly-reduced-pancreatic-beta-cell-mass-and-insulin-resistance-in-clinically-diagnosed-type-2-diabetes/) - Publication Categories: ORIGINAL Subauste A, Gianani R, Chang AM, Plunkett C, Pietropaolo S, Zhang YJ, Barinas-Mitchell E, Kuller LH, Galecki A, Halter JB, Pietropaolo M. (2014) Islet Autoimmunity Identifies a Unique Pattern of Impaired Pancreatic Beta-Cell Function, Markedly Reduced Pancreatic Beta Cell Mass and Insulin Resistance in Clinically Diagnosed Type 2 Diabetes. PLoS One.
- [Potential Viral Pathogenic Mechanism in Human Type 1 Diabetes](https://npod.org/publication/potential-viral-pathogenic-mechanism-in-human-type-1-diabetes/) - Publication Categories: ORIGINAL Schneider DA, von Herrath, MG. (2014) Potential viral pathogenic mechanism in human type 1 diabetes. Diabetologia. Oct;57(10):2009-18.
- [Coxsackievirus Can Persist in Murine Pancreas by Deletion of 5' Terminal Genomic Sequences](https://npod.org/publication/coxsackievirus-can-persist-in-murine-pancreas-by-deletion-of-5-terminal-genomic-sequences/) - Publication Categories: ORIGINAL Tracy S, Smithee S, Alhazmi A, Chapman N. (2014) Coxsackievirus can persist in murine pancreas by deletion of 5' terminal genomic sequences. J Med Virol. Aug 11.
- [New Insight on Human Type 1 Diabetes Biology: nPOD and nPOD-Transplantation](https://npod.org/publication/6322/) - Publication Categories: ORIGINAL Pugliese A, Vendrame F, Reijonen H, Atkinson MA, Campbell-Thompson M, Burke GW (2014) New Insight on Human Type 1 Diabetes Biology: nPOD and nPOD-Transplantation. Curr Diab Rep. Oct;14(10):530.
- [Repurposing cAMP-Modulating Medications to Promote β-Cell Replication](https://npod.org/publication/repurposing-camp-modulating-medications-to-promote-β-cell-replication/) - Publication Categories: ORIGINAL Zhao Z, Low YS, Armstrong NA, Hyoje Ryu J, Sun S, Arvanites AC, Hollister-Lock J, Shah NH, Weir GC, Annes JP. (2014) Repurposing cAMP-Modulating Medications to Promote β-Cell Replication. Molecular Endocrinology.
- [Pharmacological Induction of Pancreatic Islet Cell Transdifferentiation: relevance to type I diabetes](https://npod.org/publication/pharmacological-induction-of-pancreatic-islet-cell-transdifferentiation-relevance-to-type-i-diabetes/) - Publication Categories: ORIGINAL
- [Increased Immune Cell Infiltration of the Exocrine Pancreas: a possible contribution to the pathogenesis of type 1 diabetes](https://npod.org/publication/increased-immune-cell-infiltration-of-the-exocrine-pancreas-a-possible-contribution-to-the-pathogenesis-of-type-1-diabetes/) - Publication Categories: ORIGINAL Rodriguez-Calvo T, Ekwall O, Amirian N, Zapardial-Gonzalo J, and von Herrath MG (2014) Increased Immune Cell Infiltration of the Exocrine Pancreas: A Possible Contribution to the Pathogenesis of Type 1 Diabetes. Diabetes. Published online before print June 19, 104. Diabetes.
- [Blood and Islet Phenotypes Indicate Immunological Heterogeneity in Type 1 Diabetes](https://npod.org/publication/blood-and-islet-phenotypes-indicate-immunological-heterogeneity-in-type-1-diabetes/) - Publication Categories: ORIGINAL Arif S, Leete P, Nguyen V, Marks K, Nor NM, Estorninho M, Kronenberg-Versteeg D, Bingley PJ, Todd JA, Guy C, Dunger DB, Powrie J, Willcox A, Foulis AK, Richardson SJ, de Rinaldis E, Morgan NG, Lorenc A, Peakman M. (2014) Blood and islet phenotypes indicate immunological heterogeneity in type 1 diabetes. Diabetes. Jun
- [Hyaluronan and Hyaluronan Binding Proteins Accumulate in both Human Type 1 Diabetic Islets and Lymphoid Tissues and Associate with Inflammatory Cells in Insulitis](https://npod.org/publication/hyaluronan-and-hyaluronan-binding-proteins-accumulate-in-both-human-type-1-diabetic-islets-and-lymphoid-tissues-and-associate-with-inflammatory-cells-in-insulitis/) - Publication Categories: ORIGINAL Marika Bogdani, Pamela Y. Johnson, Susan Potter-Perigo, Nadine Nagy, Anthony J. Day, Paul L. Bollyky, and Thomas N. Wight (2014) Hyaluronan and Hyaluronan Binding Proteins Accumulate in both Human Type 1 Diabetic Islets and Lymphoid Tissues and Associate with Inflammatory Cells in Insulitis. Diabetes, March 27
- [The Brothers Karamazov within the CVBs](https://npod.org/publication/the-brothers-karamazov-within-the-cvbs/) - Publication Categories: REVIEW Dotta F, Sebastiani G. (2014). Enteroviral infections and development of type 1 diabetes: The Brothers Karamazov within the CVBs. Diabetes. Feb;63(2):384-6. doi: 10.2337/db13-1441.
- [Maternal Microchimerism: increased in the insulin positive compartment of type 1 diabetes pancreas but not in infiltrating immune cells or replicating islet cells](https://npod.org/publication/maternal-microchimerism-increased-in-the-insulin-positive-compartment-of-type-1-diabetes-pancreas-but-not-in-infiltrating-immune-cells-or-replicating-islet-cells/) - Publication Categories: ORIGINAL Ye J, Vives-Pi M, Gillespie KM (2014) Maternal microchimerism: increased in the insulin positive compartment of type 1 diabetes pancreas but not in infiltrating immune cells or replicating islet cells. PLoS One. Jan 31;9(1):e86985.
- [Pancreatic Pathology in Type 1 Diabetes Mellitus](https://npod.org/publication/pancreatic-pathology-in-type-1-diabetes-mellitus/) - Publication Categories: ORIGINAL
- [Immune Modulation in Humans: implications for type 1 diabetes mellitus](https://npod.org/publication/immune-modulation-in-humans-implications-for-type-1-diabetes-mellitus/) - Publication Categories: ORIGINAL Roep BO, Tree TI. (2014) Immune modulation in humans: implications for type 1 diabetes mellitus. Nat Rev Endocrinol. doi: 10.1038/nrendo.2014.2.
- [Aberrant Islet Unfolded Protein Response in Type 2 Diabetes](https://npod.org/publication/aberrant-islet-unfolded-protein-response-in-type-2-diabetes/) - Publication Categories: ORIGINAL Engin F, Nguyen T, Yermalovich A, Hotamisligil GS. (2014) Aberrant islet unfolded protein response in type 2 diabetes. Sci Rep. doi: 10.1038/srep04054.
- [Pancreatic Biopsies in Type 1 Diabetes: revisiting the myth of Pandora’s box](https://npod.org/publication/pancreatic-biopsies-in-type-1-diabetes-revisiting-the-myth-of-pandoras-box/) - Publication Categories: REVIEW Atkinson MA. (2014). Pancreatic biopsies in type 1 diabetes: revisiting the myth of Pandora’s box. Diabetologia, DOI 10.1007/s00125-013-3159-7
- [Type 1 Diabetes](https://npod.org/publication/6305/) - Publication Categories: ORIGINAL Atkinson MA, Eisenbarth GS, Michels AW. (2014). Type 1 diabetes. Lancet 383(9911):69-82.
- [B7-H4 Expression in Normal and Diseased Human Islet β Cells](https://npod.org/publication/b7-h4-expression-in-normal-and-diseased-human-islet-β-cells/) - Publication Categories: ORIGINAL Cheung SS, Ou D, Metzger DL, Meloche M, Ao Z, Ng SS, Owen D, Warnock GL. (2014) B7-H4 Expression in Normal and Diseased Human Islet β Cells. Pancreas 43(1):128-34.
- [Evaluation of the Fidelity of Immunolabelling Obtained with Clone 5D8/1, a Monoclonal Antibody Directed Against the Enteroviral Capsid Protein, VP1, in Human Pancreas](https://npod.org/publication/evaluation-of-the-fidelity-of-immunolabelling-obtained-with-clone-5d81-a-monoclonal-antibody-directed-against-the-enteroviral-capsid-protein-vp1-in-human-pancreas/) - Publication Categories: ORIGINAL
- [Enteroviruses in blood. In: “Diabetes and Viruses”](https://npod.org/publication/enteroviruses-in-blood-in-diabetes-and-viruses/) - Publication Categories: REVIEW Toniolo A , Salvatoni A, Federico G, Diaz-Horta O, Maccari G, Baj A (2013). Enteroviruses in blood. In: “Diabetes and Viruses”. K Taylor, H Hyoty, A Toniolo, A Zuckerman (Eds.), Springer, New York, 2013; pp. 143-156.
- [Human Pancreatic β-Cell G1/S Molecule Cell Cycle Atlas](https://npod.org/publication/human-pancreatic-β-cell-g1s-molecule-cell-cycle-atlas/) - Publication Categories: ORIGINAL Fiaschi-Taesch NM, Kleinberger JW, Salim FG, Troxell R, Wills R, Tanwir M, Casinelli G, Cox AE, Takane KK, Scott DK, Stewart AF. (2013) Human Pancreatic β-Cell G1/S Molecule Cell Cycle Atlas. Diabetes. 2013 Jul;62(7):2450-9.
- [Antigen-specific Immunomodulation for Type 1 Diabetes by Novel Recombinant Antibodies Directed Against Diabetes-associates Auto-reactive T Cell Epitope](https://npod.org/publication/antigen-specific-immunomodulation-for-type-1-diabetes-by-novel-recombinant-antibodies-directed-against-diabetes-associates-auto-reactive-t-cell-epitope/) - Publication Categories: ORIGINAL Dahan R, Gebe JA, Preisinger A, James EA, Tendler M, Nepom GT, Reiter Y. (2013) Antigen-specific immunomodulation for type 1 diabetes by novel recombinant antibodies directed against diabetes-associates auto-reactive T cell epitope. J Autoimmun. 2013 Dec;47:83-93.
- [Persistent C-peptide: what does it mean?](https://npod.org/publication/persistent-c-peptide-what-does-it-mean/) - Publication Categories: ORIGINAL Pietropaolo M. (2013) Persistent C-peptide: what does it mean? Curr Opin Endocrinol Diabetes Obes. Aug;20(4):279-84.
- [Expression of the Enteroviral Capsid Protein VP1 in the Islet Cells of Patients with Type 1 Diabetes is Associated with Induction of Protein Kinase R and Downregulation of Mcl-1](https://npod.org/publication/expression-of-the-enteroviral-capsid-protein-vp1-in-the-islet-cells-of-patients-with-type-1-diabetes-is-associated-with-induction-of-protein-kinase-r-and-downregulation-of-mcl-1-2/) - Publication Categories: ORIGINAL Richardson, S.J., Leete, P., Bone, A.J., Foulis, A.K., Morgan, N.G. (2013). Expression of the enteroviral capsid protein VP1 in the islet cells of patients with type 1 diabetes is associated with induction of protein kinase R and downregulation of Mcl-1. Diabetologia, 56(1): 185-193.
- [Infection of Human Islets of Langerhans with Two Strains of Coxsackie B Virus Serotype 1: assessment of virus replication, degree of cell death and induction of genes involved in the innate immunity pathway](https://npod.org/publication/infection-of-human-islets-of-langerhans-with-two-strains-of-coxsackie-b-virus-serotype-1-assessment-of-virus-replication-degree-of-cell-death-and-induction-of-genes-involved-in-the-innate-immunity-p/) - Publication Categories: ORIGINAL Anagandula M, Richardson SJ, Oberste MS, Sioofy-Khojine AB, Hyöty H, Morgan NG, Korsgren O, Frisk G. (2013). Infection of human islets of langerhans with two strains of coxsackie B virus serotype 1: Assessment of virus replication, degree of cell death and induction of genes involved in the innate immunity pathway. J Med
- [VHL-mediated Disruption of Sox9 Activity Compromises β-cell Identity and Results in Diabetes Mellitus](https://npod.org/publication/6294/) - Publication Categories: ORIGINAL Puri S, Akiyama H, Hebrok M. (2013) VHL-mediated disruption of Sox9 activity compromises β-cell identity and results in diabetes mellitus. Genes Dev. 27(23):2563-75.
- [Interaction Between Cytokines and Inflammatory Cells in Islet Dysfunction, Insulin Resistance and Vascular Disease](https://npod.org/publication/interaction-between-cytokines-and-inflammatory-cells-in-islet-dysfunction-insulin-resistance-and-vascular-disease/) - Publication Categories: ORIGINAL
- [Sphingomyelin Patches on Pancreatic Beta-cells are Indicative of Insulin Secretory Capacity](https://npod.org/publication/sphingomyelin-patches-on-pancreatic-beta-cells-are-indicative-of-insulin-secretory-capacity/) - Publication Categories: ORIGINAL Kavishwar A, Moore A. (2013) Sphingomyelin patches on pancreatic beta-cells are indicative of insulin secretory capacity. J Histochem Cytochem. 61(12):910-9.
- [Biomarkers for Immune Intervention Trials in Type 1 Diabetes](https://npod.org/publication/biomarkers-for-immune-intervention-trials-in-type-1-diabetes/) - Publication Categories: REVIEW Mallone R, Roep BO. (2013) Biomarkers for immune intervention trials in type 1 diabetes. Clin Immunol. 149(3):286-96.
- [Diminished Adenosine A1 Receptor Expression in Pancreatic α-cells May Contribute to the Pathology of Type 1 Diabetes](https://npod.org/publication/diminished-adenosine-a1-receptor-expression-in-pancreatic-α-cells-may-contribute-to-the-pathology-of-type-1-diabetes/) - Publication Categories: ORIGINAL Yip L, Taylor C, Whiting CC, Fathman CG. (2013) Diminished adenosine A1 receptor expression in pancreatic α-cells may contribute to the pathology of type 1 diabetes. Diabetes., 62(12):4208-19.
- [Response to comments on: Butler et al. Marked expansion of exocrine and endocrine pancreas with incretin therapy in humans with increased exocrine pancreas dysplasia and the potential for glucagon-producing neuroendocrine tumors](https://npod.org/publication/response-to-comments-on-butler-et-al-marked-expansion-of-exocrine-and-endocrine-pancreas-with-incretin-therapy-in-humans-with-increased-exocrine-pancreas-dysplasia-and-the-potential-for-glucagon-pro/) - Publication Categories: REVIEW Butler AE, Campbell-Thompson M, Gurlo T, Dawson DW, Atkinson M, Butler PC. (2013) Response to comments on: Butler et al. Marked expansion of exocrine and endocrine pancreas with incretin therapy in humans with increased exocrine pancreas dysplasia and the potential for glucagon-producing neuroendocrine tumors. Diabetes 2013;62:2595-2604.
- [Increased Complement Activation in Human Type 1 Diabetes Pancreata](https://npod.org/publication/increased-complement-activation-in-human-type-1-diabetes-pancreata/) - Publication Categories: ORIGINAL Rowe P, Wasserfall C, Croker B, Campbell-Thompson M, Pugliese A, Atkinson M, Schatz D. (2013) Increased complement activation in human type 1 diabetes pancreata. Diabetes Care. doi: 10.2337/dc13-0203.
- [An Analysis of Characteristics of Subjects Examined for Incretin Effects on Pancreatic Pathology](https://npod.org/publication/6286/) - Publication Categories: ORIGINAL Harja E, Lord J, Skyler JS. (2013) An analysis of characteristics of subjects examined for incretin effects on pancreatic pathology. Diabetes Technol Ther. 15(8):609-18. doi: 10.1089/dia.2013.0177.
- [Extrathymic Aire-Expressing Cells are a Distinct Bone Marrow-Derived Population that Induce Functional Inactivation of CD4+ T Cells](https://npod.org/publication/extrathymic-aire-expressing-cells-are-a-distinct-bone-marrow-derived-population-that-induce-functional-inactivation-of-cd4-t-cells/) - Publication Categories: ORIGINAL Gardner JM, Metzger TC, McMahon EJ, Au-Yeung BB, Krawisz AK, Lu W, Price JD, Johannes KP, Satpathy AT, Murphy KM, Tarbell KV, Weiss A, Anderson MS. (2013) Extrathymic Aire-Expressing Cells are a Distinct Bone Marrow-Derived Population that Induce Functional Inactivation of CD4+ T Cells Immunity. 19;39(3):560-72.
- [Insulin-dependent Diabetes Induced by Pancreatic Beta Cell Expression of IL-15 and IL-15Rα](https://npod.org/publication/insulin-dependent-diabetes-induced-by-pancreatic-beta-cell-expression-of-il-15-and-il-15rα/) - Publication Categories: ORIGINAL Chen J, Feigenbaum L, Awasthi P, Butcher DO, Anver MR, Golubeva YG, Bamford R, Zhang X, St Claire MB, Thomas CJ, Discepolo V, Jabri B, Waldmann TA. (2013) Insulin-dependent diabetes induced by pancreatic beta cell expression of IL-15 and IL-15Rα. Proc Natl Acad Sci U S A.110(33):13534-9.
- [The Case for Virus-induced Type 1 Diabetes](https://npod.org/publication/the-case-for-virus-induced-type-1-diabetes/) - Publication Categories: ORIGINAL
- [Ancillary Study Management Systems: a review of needs](https://npod.org/publication/ancillary-study-management-systems-a-review-of-needs/) - Publication Categories: REVIEW Nelson EK, Piehler B, Rauch A, Ramsay S, Holman D, Asare S, Asare A, Igra M. (2013). Ancillary study management systems: a review of needs BMC Med Inform Decis Mak., 13(5).
- [The Diagnosis of Insulitis in human Type 1 Diabetes](https://npod.org/publication/the-diagnosis-of-insulitis-in-human-type-1-diabetes/) - Publication Categories: ORIGINAL Campbell-Thompson ML, Atkinson MA, Butler AE, Chapman NM, Frisk G, Gianani R, Giepmans BN, von Herrath MG, Hyöty H, Kay TW, Korsgren O, Morgan NG, Powers AC, Pugliese A, Richardson SJ, Rowe PA, Tracy S, In't Veld PA. (2013) The diagnosis of insulitis in human type 1 diabetes. Diabetologia. 56(11):2541-3.
- [Tropism Analysis of Two Coxsackie B5 Strains Reveals Virus Growth in Human Primary Pancreatic Islets but not in Exocrine Cell Clusters In Vitro](https://npod.org/publication/tropism-analysis-of-two-coxsackie-b5-strains-reveals-virus-growth-in-human-primary-pancreatic-islets-but-not-in-exocrine-cell-clusters-in-vitro/) - Publication Categories: ORIGINAL Hodik M, Lukinius A, Korsgren O, Frisk G. (2013) Tropism Analysis of Two Coxsackie B5 Strains Reveals Virus Growth in Human Primary Pancreatic Islets but not in Exocrine Cell Clusters In Vitro. Open Virol J. 2013 Apr 5;7:49-56. doi: 10.2174/1874357901307010049. Print 2013.
- [Intrafamilial Spread of Enterovirus Infections at the Clinical Onset of Type 1 Diabetes](https://npod.org/publication/6279/) - Publication Categories: ORIGINAL Salvatoni A, Baj A, Bianchi G, Federico G, Colombo M, Toniolo A. (2013). Intrafamilial spread of enterovirus infections at the clinical onset of type 1 diabetes. Pediatr Diabetes.
- [Type 1 diabetes: translating mechanistic observations into effective clinical outcomes](https://npod.org/publication/type-1-diabetes-translating-mechanistic-observations-into-effective-clinical-outcomes/) - Publication Categories: ORIGINAL Herold KC, Vignali DA, Cooke A, Bluestone JA. (2013). Type 1 diabetes: translating mechanistic observations into effective clinical outcomes. Nat Rev Immunol., 13(4):243-56.
- [Antibody Cross-reactivity and the Viral Aetiology of Type 1 Diabetes](https://npod.org/publication/antibody-cross-reactivity-and-the-viral-aetiology-of-type-1-diabetes/) - Publication Categories: ORIGINAL Coppieters KT, von Herrath M. (2013). Antibody cross-reactivity and the viral aetiology of type 1 diabetes. J Pathol., 230(1):1-3.
- [Production and Function of IL-12 in Islets and Beta Cells](https://npod.org/publication/production-and-function-of-il-12-in-islets-and-beta-cells/) - Publication Categories: ORIGINAL Taylor-Fishwick DA, Weaver JR, Grzesik W, Chakrabarti S, Green-Mitchell S, Imai Y, Kuhn N, Nadler JL. (2013). Production and function of IL-12 in islets and beta cells. Diabetologia., 56(1):126-35.
- [Viruses and Type 1 Diabetes: a new look at an old story](https://npod.org/publication/viruses-and-type-1-diabetes-a-new-look-at-an-old-story/) - Publication Categories: ORIGINAL Craig ME, Nair S, Stein H, Rawlinson WD. (2013). Viruses and type 1 diabetes: a new look at an old story. Pediatr Diabetes., 14(3):149-58.
- [Immunological Biomarkers: catalysts for translational advances in autoimmune diabetes](https://npod.org/publication/6273/) - Publication Categories: ORIGINAL Ahmed ST, Akirav E, Bradshaw E, Buckner J, McKinney E, Quintana FJ, Waldron-Lynch F, Nepom J. (2013). Immunological biomarkers: Catalysts for translational advances in autoimmune diabetes. Clin Exp Immunol., 172(2):178-85.
- [Marked Expansion of Exocrine and Endocrine Pancreas with Incretin Therapy in Humans with Increased Exocrine Pancreas Dysplasia and the Potential for Glucagon-producing Neuroendocrine Tumors](https://npod.org/publication/6272/) - Publication Categories: ORIGINAL Butler AE, Campbell-Thompson M, Gurlo T, Dawson DW, Atkinson M, Butler PC. (2013). Marked Expansion of Exocrine and Endocrine Pancreas with Incretin Therapy in Humans with increased Exocrine Pancreas Dysplasia and the potential for Glucagon-producing Neuroendocrine Tumors. Diabetes, Mar 22.
- [Reduction of Circulating Neutrophils Precedes and Accompanies Type 1 Diabetes](https://npod.org/publication/reduction-of-circulating-neutrophils-precedes-and-accompanies-type-1-diabetes/) - Publication Categories: ORIGINAL Valle A, Giamporcaro GM, Scavini M, Stabilini A, Grogan P, Bianconi E, Sebastiani G, Masini M, Maugeri N, Porretti L, Bonfanti R, Meschi F, De Pellegrin M, Lesma A, Rossini S, Piemonti L, Marchetti P, Dotta F, Bosi E, Battaglia M. (2013). Reduction of circulating neutrophils precedes and accompanies type 1 diabetes.
- [Expression and Regulation of Chemokines in Murine and Human Type 1 Diabetes](https://npod.org/publication/expression-and-regulation-of-chemokines-in-murine-and-human-type-1-diabetes-2/) - Publication Categories: ORIGINAL
- [Hyaluronan and Versican in the Control of Human T-lymphocyte Adhesion and Migration](https://npod.org/publication/hyaluronan-and-versican-in-the-control-of-human-t-lymphocyte-adhesion-and-migration/) - Publication Categories: ORIGINAL
- [Vesicular Monoamine Transporter, Type 2 (vmat2) Expression as it Compares to Insulin and Pancreatic Polypeptide in the Head, Body and Tail of the Human Pancreas](https://npod.org/publication/vesicular-monoamine-transporter-type-2-vmat2-expression-as-it-compares-to-insulin-and-pancreatic-polypeptide-in-the-head-body-and-tail-of-the-human-pancreas/) - Publication Categories: ORIGINAL Freeby M, Ichise M, Harris PE. (2012). Vesicular monoamine transporter, type 2 (vmat2) expression as it compares to insulin and pancreatic polypeptide in the head, body and tail of the human pancreas. Islets., 4(6):393-397.
- [The Role of Hyaluronan and the Extracellular Matrix in Islet Inflammation and Immune Regulation](https://npod.org/publication/the-role-of-hyaluronan-and-the-extracellular-matrix-in-islet-inflammation-and-immune-regulation/) - Publication Categories: ORIGINAL Bollyky, P.L., Bogdani, M., Bollyky, J.B., Hull, R.L., Wight, T.N. (2012). The role of hyaluronan and the extracellular matrix in islet inflammation and immune regulation. Curr Diab Rep., 12(5):471-80
- [Pancreas Organ Weight in Individuals With Disease-Associated Autoantibodies at Risk for Type 1 Diabetes](https://npod.org/publication/pancreas-organ-weight-in-individuals-with-disease-associated-autoantibodies-at-risk-for-type-1-diabetes/) - Publication Categories: ORIGINAL Campbell-Thompson, M., Wasserfall, C., Montgomery, E.L.,Atkinson, M.A., Kaddis, J.S. (2012). Pancreas Organ Weight in Individuals With Disease-Associated Autoantibodies at Risk for Type 1 Diabetes. JAMA, 308(22):2337-2339.
- [The Peri-islet Basement Membrane, a Barrier to Infiltrating Leukocytes in Type 1 Diabetes in Mouse and Human](https://npod.org/publication/the-peri-islet-basement-membrane-a-barrier-to-infiltrating-leukocytes-in-type-1-diabetes-in-mouse-and-human/) - Publication Categories: ORIGINAL Korpos E, Kadri N, Kappelhoff R, Wegner J, Overall CM, Weber E, Holmberg D, Cardell S, Sorokin L. (2012) The Peri-islet Basement Membrane, a Barrier to Infiltrating Leukocytes in Type 1 Diabetes in Mouse and Human. Diabetes, Nov 8. Epub ahead of print.
- [Reduced DEAF1 Function During Type 1 Diabetes Inhibits Translation in Lymph Node Stromal Cells by Suppressing Eif4g3](https://npod.org/publication/reduced-deaf1-function-during-type-1-diabetes-inhibits-translation-in-lymph-node-stromal-cells-by-suppressing-eif4g3/) - Publication Categories: ORIGINAL
- [Viruses, Diabetes, and Autoimmunity: studies of subjects at genetic risk for type 1 diabetes](https://npod.org/publication/viruses-diabetes-and-autoimmunity-studies-of-subjects-at-genetic-risk-for-type-1-diabetes/) - Publication Categories: ORIGINAL Taylor, K., Hyöty, H., Toniolo, A., & Zuckerman, A. J. (2012). Viruses, diabetes, and autoimmunity: Studies of subjects at genetic risk for type 1 diabetes. Diabetes and Viruses. Springer
- [Advancing Animal Models of Human Type 1 Diabetes by Engraftment of Functional Human Tissues in Immunodeficient Mice](https://npod.org/publication/advancing-animal-models-of-human-type-1-diabetes-by-engraftment-of-functional-human-tissues-in-immunodeficient-mice/) - Publication Categories: ORIGINAL Brehm MA, Powers AC, Shultz LD, Greiner DL. (2012). Advancing animal models of human type 1 diabetes by engraftment of functional human tissues in immunodeficient mice. Cold Spring Harb Perspect Med. 2012 May;2(5):a007757. doi: 10.1101/cshperspect.a007757.
- [Formation of a Human ß-cell Population within Pancreatic Islets is Set Early in Life](https://npod.org/publication/formation-of-a-human-s-cell-population-within-pancreatic-islets-is-set-early-in-life/) - Publication Categories: ORIGINAL Gregg, B. E., Moore, P. C., Demozay, D., Hall, B. A., Li, M., Husain, A., Wright, A. J., Atkinson, M. A., Rhodes, C. J. (2012). Formation of a human ß-cell population within pancreatic islets is set early in life. Journal of Clinical Endocrinology & Metabolism, 97(9), 3197-3206.
- [Expression of Endoplasmic Reticulum Stress Markers in the Islets of Patients with Type 1 Diabetes](https://npod.org/publication/expression-of-endoplasmic-reticulum-stress-markers-in-the-islets-of-patients-with-type-1-diabetes/) - Publication Categories: ORIGINAL
- [Collection Protocol for Human Pancreas](https://npod.org/publication/collection-protocol-for-human-pancreas/) - Publication Categories: ORIGINAL Campbell-Thompson, M., Montgomery, E., Foss, R., Kolheffer, K., Phipps, G., Schneider, L., Atkinson, M. (2012). Collection protocol for human pancreas. Journal of Visual Experiments, 63, video article.
- [Staining Protocols for Human Pancreatic Islets](https://npod.org/publication/staining-protocols-for-human-pancreatic-islets/) - Publication Categories: ORIGINAL Campbell-Thompson, M., Heiple, T., Montgomery, E., Zhang, L., Schneider, L. (2012). Staining protocols for human pancreatic islets. Journal of Visual Experiments, 63, video article.
- [Network for Pancreatic Organ Donors with Diabetes (nPOD): Developing a tissue biobank for type 1 diabetes](https://npod.org/publication/network-for-pancreatic-organ-donors-with-diabetes-npod-developing-a-tissue-biobank-for-type-1-diabetes/) - Publication Categories: ORIGINAL Campbell-Thompson, M., Wasserfall, C., Kaddis, J., Albanese-O'Neill, A., Staeva, T., Nierras, C., Moraski, J., Rowe, P., Gianani, R., Eisenbarth, G., Crawford, J., Schatz, D., Pugliese, A., Atkinson, M. (2012). Network for pancreatic organ donors with diabetes (nPOD): Developing a tissue biobank for type 1 diabetes. Diabetes/Metabolism Research and Reviews, 28.
- [Islet-enriched Gene Expression and Glucose-induced Insulin Secretion in Human and Mouse Islets](https://npod.org/publication/islet-enriched-gene-expression-and-glucose-induced-insulin-secretion-in-human-and-mouse-islets/) - Publication Categories: ORIGINAL
- [Demonstration of Islet-autoreactive CD8 T Cells in Insulitic Lesions from Recent Onset and Long-term Type 1 Diabetes Patients](https://npod.org/publication/demonstration-of-islet-autoreactive-cd8-t-cells-in-insulitic-lesions-from-recent-onset-and-long-term-type-1-diabetes-patients/) - Publication Categories: ORIGINAL Coppieters, K. T., Dotta, F., Amirian, N., Campbell, P. D., Kay, T. W., Atkinson, M. A., Roep, B. O., & von Herrath, M. G. (2012). Demonstration of islet-autoreactive CD8 T cells in insulitic lesions from recent onset and long-term type 1 diabetes patients. J Exp Med., 209 (1):51-60.
- [Beta Cells Under Attack: toward a better understanding of type 1 diabetes immunopathology](https://npod.org/publication/beta-cells-under-attack-toward-a-better-understanding-of-type-1-diabetes-immunopathology/) - Publication Categories: ORIGINAL Coppieters KT, Roep BO, von Herrath MG. (2011). Beta cells under attack: toward a better understanding of type 1 diabetes immunopathology. Semin Immunopathol., 33 (1):1-7.
- [Humanized Mice for the Study of Type 1 and Type 2 Diabetes](https://npod.org/publication/humanized-mice-for-the-study-of-type-1-and-type-2-diabetes/) - Publication Categories: ORIGINAL Greiner, D. L., Brehm, M. A., Hosur, V., Harlan, D. M., Powers, A. C., & Shultz L. D. (2011). Humanized mice for the study of type 1 and type 2 diabetes. Ann NY Acad Sci., 1245 (1):55-58.
- [Persistent Glucose Transporter Expression on Pancreatic Beta Cells from Longstanding Type 1 Diabetic Individuals](https://npod.org/publication/persistent-glucose-transporter-expression-on-pancreatic-beta-cells-from-longstanding-type-1-diabetic-individuals/) - Publication Categories: ORIGINAL Coppieters, K. T., Wiber, A., Amirian, N., Kay, T. W., & von Herrath, M. G. (2011). Persistent glucose transporter expression on pancreatic beta cells from longstanding type 1 diabetic individuals. Diabetes/Metabolism Research and Reviews, 27, 746-754.
- [On-tissue Identification of Insulin: In situ reduction coupled with mass spectrometry imaging](https://npod.org/publication/on-tissue-identification-of-insulin-in-situ-reduction-coupled-with-mass-spectrometry-imaging/) - Publication Categories: ORIGINAL
- [The Pancreas in Human Type 1 Diabetes](https://npod.org/publication/the-pancreas-in-human-type-1-diabetes/) - Publication Categories: ORIGINAL Rowe, P. A., Campbell-Thompson, M. L., Schatz, D. A., & Atkinson, M. A. (2011). The pancreas in human type 1 diabetes. Seminars in Immunopathology.
- [Dimorphic Histopathology of Long Standing Childhood-onset Diabetes](https://npod.org/publication/dimorphic-histopathology-of-long-standing-childhood-onset-diabetes/) - Publication Categories: ORIGINAL
- [Residual Insulin Production and Pancreatic ß-cell Turnover after 50 years of Diabetes: Joslin Medalist Study](https://npod.org/publication/residual-insulin-production-and-pancreatic-s-cell-turnover-after-50-years-of-diabetes-joslin-medalist-study/) - Publication Categories: ORIGINAL Keenan, H.A., Sun, J.K., Levine, J., Doria, A., Aiello, L.P., Eisenbarth, G., Bonner-Weir, S., & King, G.L. (2010). Residual insulin production and pancreatic ß-cell turnover after 50 years of diabetes: Joslin Medalist Study. Diabetes, 59(11), 2846-53.
- [Prevention of Diabetes by FTY720-Mediated Stabilization of Peri-islet Tertiary Lymphoid Organs](https://npod.org/publication/prevention-of-diabetes-by-fty720-mediated-stabilization-of-peri-islet-tertiary-lymphoid-organs/) - Publication Categories: ORIGINAL
- [Genetic Control of ß-cell Mass Homeostasis](https://npod.org/publication/genetic-control-of-s-cell-mass-homeostasis/) - Publication Categories: ORIGINAL
- [Deaf1 Isoforms Control the Expression of Genes Encoding Peripheral Tissue Antigens in the Pancreatic Lymph Nodes During Type 1 Diabetes](https://npod.org/publication/deaf1-isoforms-control-the-expression-of-genes-encoding-peripheral-tissue-antigens-in-the-pancreatic-lymph-nodes-during-type-1-diabetes/) - Publication Categories: ORIGINAL
- [The Pancreas in Human Type 1 Diabetes: providing new answers to age-old questions](https://npod.org/publication/the-pancreas-in-human-type-1-diabetes-providing-new-answers-to-age-old-questions/) - Publication Categories: ORIGINAL Atkinson, M.A. & Gianani, R. (2009) The pancreas in human type 1 diabetes: providing new answers to age-old questions. Current Opinion in Endocrinology, Diabetes & Obesity, 16(4), 279-85.
- [Post-mortem Analysis of Islet Pathology in Type 1 Diabetes Illuminates the Life and Death of the ß Cell](https://npod.org/publication/post-mortem-analysis-of-islet-pathology-in-type-1-diabetes-illuminates-the-life-and-death-of-the-s-cell/) - Publication Categories: ORIGINAL Spencer, J. & Peakman, M. (2008). Post-mortem analysis of islet pathology in type 1 diabetes illuminates the life and death of the ß cell. Clinical and Experimental Immunology, 155, 125-127.
- [Diabetes: A virus-gene collaboration](https://npod.org/publication/diabetes-a-virus-gene-collaboration/) - Publication Categories: ORIGINAL Von Herrath, Matthias. (2009). Diabetes: A virus-gene collaboration. Nature, 459, 518-19.

## Categories

- [Uncategorized](https://npod.org/category/uncategorized/)
- [News & Events](https://npod.org/category/news-events/)
- [Publications](https://npod.org/category/publications/)
- [Home Slideshow](https://npod.org/category/home-slideshow/)
- [Current nPOD Projects](https://npod.org/category/current-npod-projects/)
- [Beta Cell Development, Differentiation & Regeneration](https://npod.org/category/current-npod-projects/beta-cell-development-differentiation-regeneration/)
- [Beta Cell Physiology and Dysfunction](https://npod.org/category/current-npod-projects/beta-cell-physiology-and-dysfunction/)
- [Core Lab](https://npod.org/category/current-npod-projects/core-lab/)
- [Immunology](https://npod.org/category/current-npod-projects/immunology/)
- [Novel Biomarkers](https://npod.org/category/current-npod-projects/novel-biomarkers/)
- [Pathology](https://npod.org/category/current-npod-projects/pathology/)
- [Type 1 Diabetes Etiology & Environment](https://npod.org/category/current-npod-projects/type-1-diabetes-etiology-environment/)
- [Archived Projects](https://npod.org/category/current-npod-projects/archived-projects/)
- [Novel Technologies](https://npod.org/category/current-npod-projects/novel-technologies/)
- [nPOD Webinars](https://npod.org/category/npod-webinars/)
- [Past Webinars](https://npod.org/category/npod-webinars/past-webinars/)
- [Exocrine Pancreas](https://npod.org/category/current-npod-projects/exocrine-pancreas/)
- [Alpha Cell Physiology & Dysfunction](https://npod.org/category/current-npod-projects/alpha-cell-physiology-dysfunction/)
- [Featured](https://npod.org/category/news-events/featured/)

## Tags

- [Publications](https://npod.org/tag/publications/)
- [Martha Campbell-Thompson](https://npod.org/tag/martha-campbell-thompson/)

## Event Abstract Categories

- [Beta Cell Physiology and Dysfunction](https://npod.org/event_abstract_category/beta-cell-physiology-and-dysfunction/)
- [Beta Cell Development, Differentiation & Regeneration](https://npod.org/event_abstract_category/beta-cell-development-differentiation-regeneration/)
- [Immunology](https://npod.org/event_abstract_category/immunology/)
- [Novel Biomarkers](https://npod.org/event_abstract_category/novel-biomarkers/)
- [Novel Technologies](https://npod.org/event_abstract_category/novel-technologies/)
- [Pathology](https://npod.org/event_abstract_category/pathology/)

## Publication Categories

- [ORIGINAL](https://npod.org/publication_category/original/)
- [REVIEW](https://npod.org/publication_category/review/)
- [Letter to the Editor](https://npod.org/publication_category/letter-to-the-editor/)
- [COMMENT](https://npod.org/publication_category/comment/)
- [PERSPECTIVE](https://npod.org/publication_category/perspective/)